Cell Biology

Noncanonical roles of ATG5 and membrane atg8ylation in retromer assembly and function

1. Masroor Ahmad Paddar
2. Fulong Wang
3. Einar S Trosdal
4. Emily Hendrix
5. Yi He
6. Michelle R Salemi
7. Michal Mudd
8. Jingyue Jia
9. Thabata Duque
10. Ruheena Javed
11. Brett S Phinney
12. Vojo Deretic

• Curated by eLife

  eLife Assessment

  Masroor Ahmad Paddar and colleagues reveal noncanonical roles of ATG5 and membrane ATG8ylation in regulating retromer assembly and function. They identify ATG5's unique non-autophagic role and show that CASM partially contributes to these phenotypes. Although the mechanism by which ATG8ylation regulates the retromer remains unclear, the findings provide important insights with solid supporting evidence.

Reviewed by eLife

Rejuvenating aged osteoprogenitors for bone repair

1. Joshua Reeves
2. Pierre Tournier
3. Pierre Becquart
4. Robert Carton
5. Yin Tang
6. Alessandra Vigilante
7. Dong Fang
8. Shukry J Habib

• Curated by eLife

  eLife Assessment

  Aging reduces tissue regeneration capacity, posing challenges for an aging population. In this fundamental study, Reeves et al. show that by combining Wnt-mediated osteoprogenitor expansion (using a special bandage) with intermittent fasting, calvarial bone healing can be restored in aged animals. Intermitted fasting improves osteoprogenitor function by rescuing aging-related mitochondrial dysfunction, which can also be achieved by nicotinamide mononucleotide (NMN) supplementation or by modulating the gut microbiome. By employing rigorous histological, transcriptomic, and imaging analyses in a clinically relevant model, the authors provide compelling evidence supporting the conclusions. The therapeutic approach presented in this study shows promise for rejuvenating tissue repair, not only in bones but potentially across other tissues.

Reviewed by eLife

Leukocytes use endothelial membrane tunnels to extravasate the vasculature

1. Werner J. van der Meer
2. Abraham C.I. van Steen
3. Eike Mahlandt
4. Loïc Rolas
5. Haitao Wang
6. Janine J.G. Arts
7. Lanette Kempers
8. Max L.B. Grönloh
9. Rianne M. Schoon
10. Amber Driessen
11. Jos van Rijssel
12. Ingeborg Klaassen
13. Reinier O. Schlingemann
14. Yosif Manavski
15. Mark Hoogenboezem
16. Reinier A. Boon
17. Satya Khuon
18. Eric Wait
19. John Heddleston
20. Teng-Leong Chew
21. Martijn A. Nolte
22. Sussan Nourshargh
23. Joachim Goedhart
24. Jaap D. van Buul

Reviewed by preLights

SpotMAX: a generalist framework for multi-dimensional automatic spot detection and quantification

1. Francesco Padovani
2. Ivana Čavka
3. Ana Rita Rodrigues Neves
4. Cristina Piñeiro López
5. Nada Al-Refaie
6. Leonardo Bolcato
7. Dimitra Chatzitheodoridou
8. Yagya Chadha
9. Xiaofeng A. Su
10. Jette Lengefeld
11. Daphne S. Cabianca
12. Simone Köhler
13. Kurt M. Schmoller

Reviewed by Arcadia Science

Genome-wide analysis of anterior-posterior mRNA regionalization in Stentor coeruleus reveals a role for the microtubule cytoskeleton

1. Ashley R. Albright
2. Connie Yan
3. David Angeles-Albores
4. Tatyana Makushok
5. Jamarc Allen-Henderson
6. Wallace F. Marshall

Reviewed by Arcadia Science

Calcium tunneling through the ER and transfer to other organelles for optimal signaling in Toxoplasma gondii

1. Zhu-Hong Li
2. Beejan Asady
3. Le Chang
4. Miryam Andrea Hortua Triana
5. Catherine Li
6. Isabelle Coppens
7. Silvia NJ Moreno

• Curated by eLife

  eLife Assessment

  This study provides valuable new information on the role of the endoplasmic reticulum calcium pump, TgSERCA, in the human pathogen Toxoplasma gondii. It is proposed that the endoplasmic reticulum is the major calcium store in these protists and that calcium is directly transported to other organelles via membrane contact sites. While the experimental work is solid and supported by complementary approaches, direct evidence for intra-organellar calcium transport via membrane domains and specific calcium efflux transporters is lacking.

Reviewed by eLife

Constitutively active receptor ADGRA3 signaling induces adipose thermogenesis

1. Zewei Zhao
2. Longyun Hu
3. Bigui Song
4. Tao Jiang
5. Qian Wu
6. Jiejing Lin
7. Xiaoxiao Li
8. Yi Cai
9. Jin Li
10. Bingxiu Qian
11. Siqi Liu
12. Jilu Lang
13. Zhonghan Yang

• Curated by eLife

  eLife Assessment

  The study highlights adhesion G-protein-coupled receptor A3 (ADGRA3) as a potential target for activating adaptive thermogenesis in both white and brown adipose tissue. This finding offers valuable insights for researchers in the field of adipose tissue biology and metabolism. The authors have presented additional evidence to address the reviewers' comments, including experiments conducted on primary stromal vascular fractions from adipose tissues. However, the revised manuscript fails to address several reviewer concerns, such as the measurement of whole-body energy expenditure through indirect calorimetry and the assessment of food intake. Furthermore, the nanoparticle-mediated knockdown of Adgra3 did not adequately address the tissue selectivity of ADGRA in mice. As a result, the primary claims of the study are only partially supported by the available data, leading to the conclusion that the research is deemed incomplete.

Reviewed by eLife

Impairment of lipid homeostasis causes lysosomal accumulation of endogenous protein aggregates through ESCRT disruption

1. John Yong
2. Jacqueline E Villalta
3. Ngoc Vu
4. Matthew A Kukurugya
5. Niclas Olsson
6. Magdalena Preciado López
7. Julia R Lazzari-Dean
8. Kayley Hake
9. Fiona E McAllister
10. Bryson D Bennett
11. Calvin H Jan

• Curated by eLife

  eLife Assessment

  Protein and lipid homeostasis is essential for maintaining cellular functions but their crosstalk remains largely unknown. This important manuscript deals with this interesting topic and applies the powerful unbiased tools of somatic cell genetics to discover evidence suggesting a link between sphingolipids/cholesterol ester metabolism and lysosomal protein aggregation. The authors provide compelling orthogonal evidence to support their conclusions.

Reviewed by eLife

PEBP1 amplifies mitochondrial dysfunction-induced integrated stress response

1. Ling Cheng
2. Ian Meliala
3. Yidi Kong
4. Jingyuan Chen
5. Christopher G Proud
6. Mikael Björklund

• Curated by eLife

  eLife Assessment

  In this article, Cheng et al present an important finding that advances the understanding of mitochondrial stress response(s). The authors employed mass spectrometry-based methods in conjunction with standard molecular and cellular biology techniques to provide compelling evidence that phosphatidylethanolamine-binding protein 1 (PEBP1) acts as a pivotal regulator of the mitochondrial component of integrated stress response. Notwithstanding that this discovery is likely to be of significant interest to researchers across a broad spectrum of disciplines ranging from cell biology to neuroscience, it was thought that further mechanistic dissection of the role of PEBP1 in modulating integrated stress response may further strengthen this study.

Reviewed by eLife

Single-molecule lipid biosensors mitigate inhibition of endogenous effector proteins

1. Victoria L. Holmes
2. Morgan M.C. Ricci
3. Claire C. Weckerly
4. Michael Worcester
5. Gerald R.V. Hammond

Reviewed by Review Commons