Cell Biology

MARK2 regulates Golgi apparatus reorientation by phosphorylation of CAMSAP2 in directional cell migratio

1. Peipei Xu
2. Rui Zhang
3. Zhengrong Zhou
4. Honglin Xu
5. Yuejia Li
6. Mengge Yang
7. Ruifan Lin
8. Yingchun Wang
9. Xiahe Huang
10. Qi Xie
11. Wenxiang Meng

• Curated by eLife

  eLife Assessment

  The authors propose that the kinase MARK2 regulates the Golgi's reorientation towards the cell's leading edge through the regulation of microtubule binding protein CAMSAP2 and its binding to USO1. While the model is interesting and the study is useful, the quantification of an insufficient number of cells and insufficient description of the methods and biological replicates mean the results are inadequate to support the model.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife

Periosteal skeletal stem cells can migrate into the bone marrow and support hematopoiesis after injury

1. Tony Marchand
2. Kemi E Akinnola
3. Shoichiro Takeishi
4. Maria Maryanovich
5. Sandra Pinho
6. Julien Saint-Vanne
7. Alexander Birbrair
8. Thierry Lamy
9. Karin Tarte
10. Paul Frenette
11. Kira Gritsman

• Curated by eLife

  eLife Assessment

  The study presents valuable insights into the role of periosteal stem cells in bone marrow regeneration. The evidence is convincing. The data broadly support their claims and in line with state-of-art methodology. Future study on their model will help to strengthen their discovery further.

Reviewed by eLife

Rudhira-mediated microtubule stability controls TGFβ signaling during mouse vascular development

1. Divyesh Joshi
2. Preeti Jindal
3. Ronak K Shetty
4. Maneesha S Inamdar

• Curated by eLife

  eLife Assessment

  This important work provides another layer of regulatory mechanism for TGF-beta signaling activity. The evidence convincingly supports the involvement of microtubules as a reservoir of Smad2/3, and association of Rudhira with microtubules is critical for this process. The work will be of board interest to developmental biologists in general and molecular biologists in the field of growth factor signaling.

Reviewed by eLife

Probing metazoan polyphosphate biology using Drosophila reveals novel and conserved polyP functions

1. Sunayana Sarkar
2. Harsha Sharma
3. SK Yasir Hosen
4. Jayashree S Ladke
5. Deepa Balasubramanian
6. Sreejith Raran-Kurussi
7. Rashna Bhandari
8. Manish Jaiswal

• Curated by eLife

  eLife Assessment

  Studying the biological roles of polyphosphates in metazoans has been a longstanding challenge to the field given that the polyP synthase has yet to be discovered in metazoans. This important study capitalizes on the sophisticated genetics available in the Drosophila system and uses a combination of methodologies to start to tease apart how polyphosphate participates in Drosophila development and in the clotting of Drosophila hemolymph. The data validating the tools are solid and well-documented and they will open up a field of research into the functional roles of polyP in a metazoan model.

Reviewed by eLife

Macromolecular condensation is unlikely to buffer intracellular osmolality

1. Alan R. Kay
2. Zahra Aminzare

• Curated by Biophysics Colab

  Evaluation Statement (30 January 2025)

  Kay and Aminzare discuss a claim made in a prior publication that macromolecular condensation acts as a water buffering mechanism in cells to compensate for the effects of osmotic shock. The authors argue that, although such a buffer could temporarily maintain a transmembrane osmolality differential, this differential would drive water across the membrane to reach a steady-state in which osmolality within the cell equals osmolality outside the cell. Using the well-established pump-leak model for osmotic water transport, they further show that the timescale at which a water buffer could maintain a modest 10% osmolality differential across the membrane is at most one minute for a typical animal cell.

  Biophysics Colab recommends this study to researchers working on membrane transport, intracellular water buffering, and condensate biology.

  Biophysics Colab has evaluated this study as one that meets the following criteria:

  • Rigorous methodology
  • Transparent reporting
  • Appropriate interpretation

Reviewed by Biophysics Colab

The full-length BEND2 protein is dispensable for spermatogenesis but required for setting the ovarian reserve in mice

1. Yan Huang
2. Nina Bucevic
3. Carmen Coves
4. Natalia Felipe-Medina
5. Marina Marcet-Ortega
6. Nikoleta Nikou
7. Cristina Madrid-Sandín
8. Maria López-Panadés
9. Carolina Buza
10. Neus Ferrer Miralles
11. Antoni Iborra
12. Anna Pujol
13. Alberto M Pendás
14. Ignasi Roig

• Curated by eLife

  eLife Assessment

  This study provides valuable information on a novel gene that regulates meiotic progression in both male and female meiosis. The evidence supporting the conclusions of the authors is solid. This study will be of interest to developmental and reproductive biologists.

Reviewed by eLife

TXNIP mediates LAT1/SLC7A5 endocytosis to reduce amino acid uptake in cells entering quiescence

1. Jennifer Kahlhofer
2. Nikolas Marchet
3. Brigitta Seifert
4. Kristian Zubak
5. Madlen Hotze
6. Anna-Sophia Egger
7. Claudia Manzl
8. Yannick Weyer
9. Sabine Weys
10. Martin Offterdinger
11. Sebastian Herzog
12. Veronika Reiterer
13. Marcel Kwiatkowski
14. Saskia B. Wortmann
15. Siamak Nemati
16. Johannes A. Mayr
17. Johannes Zschocke
18. Bernhard Radlinger
19. Kathrin Thedieck
20. Lukas A. Huber
21. Hesso Farhan
22. Mariana E.G. de Araujo
23. Susanne Kaser
24. Sabine Scholl-Bürgi
25. Daniela Karall
26. David Teis

Reviewed by Review Commons

Albendazole specifically disrupts microtubules and protein turnover in the tegument of the cestode Mesocestoides corti

1. Inés Guarnaschelli
2. Uriel Koziol

Reviewed by Arcadia Science

Evaluation of Prostaglandin Receptor Agonists and Eupatilin in the Context of Nephronophthisis

1. Alice Tata
2. Guillaume Rocha
3. Marguerite Hureaux
4. Alice Serafin
5. Esther Porée
6. Lucie Menguy
7. Nicolas Goudin
8. Nicolas Cagnard
9. Lilian Gréau
10. Marc Fila
11. Luis Briseno-Roa
12. Jean-Philippe Annereau
13. Sophie Saunier
14. Alexandre Benmerah

Reviewed by Arcadia Science

Targeting IRE1α improves insulin sensitivity and thermogenesis and suppresses metabolically active adipose tissue macrophages in male obese mice

1. Dan Wu
2. Venkateswararao Eeda
3. Zahra Maria
4. Komal Rawal
5. Audrey Wang
6. Oana Herlea-Pana
7. Ram Babu Undi
8. Hui-Ying Lim
9. Weidong Wang

• Curated by eLife

  eLife Assessment

  The study presents important findings on inositol-requiring enzyme (IRE1α) inhibition on diet-induced obesity (overnutrition) and insulin resistance where IRE1α inhibition enhances thermogenesis and reduces the metabolically active and M1-like macrophages in adipose tissue. The evidence supporting the conclusions is convincing. The work will be of interest to cell biologists and biochemists working in metabolism, insulin resistance and inflammation with a broad eLife readership.

Reviewed by eLife