Cell Biology

Global coordination of protrusive forces in migrating immune cells

1. Patricia Reis-Rodrigues
2. Nikola Canigova
3. Mario J. Avellaneda
4. Florian Gaertner
5. Kari Vaahtomeri
6. Michael Riedl
7. Jack Merrin
8. Robert Hauschild
9. Yoshinori Fukui
10. Alba Juanes Garcia
11. Michael Sixt

Reviewed by preLights

Pharmacologically inducing regenerative cardiac cells by small molecule drugs

1. Wei Zhou
2. Kezhang He
3. Chiyin Wang
4. Pengqi Wang
5. Dan Wang
6. Bowen Wang
7. Han Geng
8. Hong Lian
9. Tianhua Ma
10. Yu Nie
11. Sheng Ding

• Curated by eLife

  eLife Assessment

  This manuscript offers valuable information on the combinatory effect of small molecules, CHIR99021 and A-485 (2C), during the reprogramming of mature cardiomyocytes into regenerative cardiac cells on stimulating cardiac cell regeneration. Although the study used several hESC lines and an in vivo model of myocardial injury to demonstrate the regenerative potential of cardiac cells, the manuscript is still incomplete as several concerns remain unanswered, including the lack of validation of the conclusions from scRNA-seq. It is still unclear how a small fraction of dedifferentiating cardiac cells can offer such broad effects on regeneration both in vitro and in vivo. If validated, this study might unlock potential therapeutic strategies for cardiac regeneration.

Reviewed by eLife

Centrosome Migration and Apical Membrane Formation in Polarized Epithelial Cells: Insights from the MDCK Cyst Model

1. Po-Kai Wang
2. Keng-Hui Lin
3. Tang K Tang

• Curated by eLife

  eLife Assessment

  Wang et al's study addresses an important critical gap in our understanding of de novo epithelial polarization using MDCK cell doublets surrounded by ECM, providing convincing evidence through imaging and depletion studies on the role of conserved polarity proteins and the centrosome during this process. While the authors propose a clear hierarchical model, there is a need for further exploration of how microtubule organization contributes to this process. Specifically, live cell imaging of microtubules under mutants and their included ECM conditions, along with a more precise temporal mapping of microtubule dynamics in relation to proteins like Gp135, would strengthen the study's conclusions.

Reviewed by eLife

Ciliary length regulation by intraflagellar transport in zebrafish

1. Yi Sun
2. Zhe Chen
3. Minjun Jin
4. Haibo Xie
5. Chengtian Zhao

• Curated by eLife

  eLife Assessment

  The manuscript represents a valuable conceptual and technical contribution to our understanding of ciliogenesis and intraflagellar transport in vertebrates. Through a series of solid and technically superb live imaging experiments to directly visualize intraflagellar transport in various zebrafish ciliated tissues, the authors unveil the surprising breadth of intraflagellar transport speed among differing organs and link this to cell type-specific differences in cilia length and intraflagellar transport train size. This work will be of broad interest to researchers in numerous fields, including development, cell biology, and imaging.

Reviewed by eLife

Epigenetic deprogramming driven by disruption of CIZ1-RNA nuclear assemblies in early-stage breast cancers

1. Dawn Coverley

Reviewed by PREreview

Mechanical Centrosome Fracturing during Cell Navigation

1. Madeleine T. Schmitt
2. Mauricio J.A. Ruiz-Fernandez
3. Kasia Stefanowski
4. Janina Kroll
5. Robert Hauschild
6. Jack Merrin
7. Thomas Penz
8. Eva Kiermaier
9. Christoph Bock
10. Tobias Straub
11. Jörg Renkawitz

Reviewed by Arcadia Science

Quantitative Comparison of Monomeric StayGold Variants Using Protein Nanocages in Living Cells

1. Giulia Viola
2. Kyle A. Jacobs
3. Joël Lemière
4. Matthew L. Kutys
5. Torsten Wittmann

Reviewed by Arcadia Science

Visualizing sarcomere and cellular dynamics in skeletal muscle to improve cell therapies

1. Judith Hüttemeister
2. Franziska Rudolph
3. Michael H Radke
4. Claudia Fink
5. Dhana Friedrich
6. Stephan Preibisch
7. Martin Falcke
8. Eva Wagner
9. Stephan E Lehnart
10. Michael Gotthardt

• Curated by eLife

  eLife Assessment

  This study offers valuable information on how titin derived from different nuclei within the syncytium is organized and integrated during skeletal muscle development and remodeling. The authors developed a novel mCherry titin knock-in mice with the fluorophore mCherry inserted into titin's Z-disk region to track the titin during cell fusion. The approach using mcherry adds to understanding of the role and localization of titin in controlling stiffness of striated muscles and fine tuning contraction. The results demonstrate that the integration of titin into the sarcomere is tightly regulated, with its unexpected mobility aiding in the uniform distribution of titin post-cell fusion. Although the experimental approach is convincing, the work is very qualitative in its approaches, and the data needs rigorous statistical analysis. There is a need for some clarification concerning numbers of animals and control groups. Future studies will need more rigorous data analysis and interpretation.

Reviewed by eLife

Endogenous oligomer formation underlies DVL2 condensates and promotes Wnt/β-catenin signaling

1. Senem Ntourmas
2. Martin Sachs
3. Petra Paclíková
4. Martina Brückner
5. Vítězslav Bryja
6. Jürgen Behrens
7. Dominic B Bernkopf

• Curated by eLife

  eLife Assessment

  This valuable study contributes to the understanding of phase separation in Dishevelled (DVL) proteins, by investigating the endogenous complexes of DVL2 using ultracentrifugation and contrasting them with DVL1 and DVL3 behaviour and the functional validation of the DVL2 intrinsically disordered regions mediating the protein condensate. The study includes a solid characterisation of several overexpression constructs, including in KO cells. However, investigations of the roles of the described DVL2 regions at the endogenous level remain to be carried out.

Reviewed by eLife

GDF2 and BMP10 coordinate liver cellular crosstalk to maintain liver health

1. Dianyuan Zhao
2. Ziwei Huang
3. Xiaoyu Li
4. Huan Wang
5. Qingwei Hou
6. Yuyao Wang
7. Fang Yan
8. Wenting Yang
9. Di Liu
10. Shaoqiong Yi
11. Chunguang Han
12. Yanan Hao
13. Li Tang

• Curated by eLife

  eLife Assessment

  This valuable study delineates the cellular contributions of BMP signaling in liver development and function. The findings are convincing, and the study employs state-of-the-art molecular, genetic, and cellular approaches to demonstrate that hepatic stellate cells play a central role in liver health by mediating cell-to-cell crosstalk via the production of specific BMP proteins. This study will be of interest to scientists interested in developmental biology and organ physiology.

Reviewed by eLife