Cell Biology

Membrane potential modulates ERK activity and cell proliferation

1. Mari Sasaki
2. Masanobu Nakahara
3. Takuya Hashiguchi
4. Fumihito Ono

• Curated by eLife

  eLife Assessment

  This important paper employs multiple experimental approaches and presents evidence that changes in membrane voltage directly affect ERK signaling to regulate cell division. This result is relevant because it supports an ion channel-independent pathway by which changes in membrane voltage can affect cell growth. The reviewers point out that some experimental results and interpretations are compelling, but the strength of evidence is incomplete and additional experiments are needed to rule out other possible interpretations of the data.

Reviewed by eLife

Intracellular diffusion in the cytoplasm increases with cell size in fission yeast

1. Catherine Tan
2. Michael C. Lanz
3. Matthew Swaffer
4. Jan Skotheim
5. Fred Chang

Reviewed by preLights

PKA regulation of neuronal function requires the dissociation of catalytic subunits from regulatory subunits

1. Weihong Xiong
2. Maozhen Qin
3. Haining Zhong

• Curated by eLife

  eLife Assessment

  This important paper demonstrates that different PKA subtypes exhibit distinct subcellular localization at rest in CA1 neurons. The authors provide compelling evidence that when all tested PKA subtypes are activated by norepinephrine, catalytic subunits translocate to dendritic spines but regulatory subunits remain unmoved. Furthermore, PKA-dependent regulation of synaptic plasticity and transmission can be supported only by wildtype, dissociable PKA, but not by inseparable PKA.

Reviewed by eLife

Spatiotemporal recruitment of the ubiquitin-specific protease USP8 directs endosome maturation

1. Yue Miao
2. Yongtao Du
3. Baolei Wang
4. Jingjing Liang
5. Yu Liang
6. Song Dang
7. Jiahao Liu
8. Dong Li
9. Kangmin He
10. Mei Ding

• Curated by eLife

  eLife Assessment

  The manuscript presents an important model for the field of endosome maturation, providing perspective on the role of the deubiquitinating enzyme UPS-50/USP8 in the process. The evidence presented in the paper is clear, incorporating well-designed experiments that suggest the dual actions of UPS-50 and USP8 in the conversion of early endosomes into late endosomes. Overall, the work is convincing and centers on an intriguing subject.

Reviewed by eLife

Non-disruptive inducible labeling of ER-membrane contact sites using the Lamin B Receptor

1. Laura Downie
2. Nuria Ferrandiz
3. Megan Jones
4. Stephen J. Royle

Reviewed by preLights

Golgi compaction facilitates microtubule nucleation to drive adult vertebrate peripheral neuron regeneration

1. Alice E Mortimer
2. Adam J Reid
3. Raman M Das

Reviewed by preLights

LAPTM4B Alleviates Pulmonary Fibrosis by Enhancing NEDD4L-Mediated TGF-β Signaling Suppression

1. Kai Xu
2. Xiaoyue Pan
3. Hui Lian
4. Yaxuan Wang
5. Ruyan Wan
6. Zhongzheng Li
7. Xin Pan
8. Yajun Li
9. Juntang Yang
10. Ivan Rosas
11. Lan Wang
12. Guoying Yu

• Curated by eLife

  eLife Assessment

  In this valuable manuscript, the authors propose that the lysosomal protein LAPTM4B plays a role in suppressing the TGF-β/SMAD signaling pathway and suggest that enhancing LAPTM4B function could be a potential therapeutic strategy for alleviating bleomycin-induced lung fibrosis. The findings will be of interest to the lung disease field, and the data presented to support the authors' conclusions is solid. However, it remains unclear whether the suppressive effect of LAPTM4b on idiopathic pulmonary fibrosis is mediated by Nedd4l.

Reviewed by eLife

Cigarette smoke impairs the endocytotic process in Saccharomyces cerevisiae

1. Aditya Shukla
2. Srimonti Sarkar
3. Alok Kumar Sil

Reviewed by Arcadia Science

Global coordination of protrusive forces in migrating immune cells

1. Patricia Reis-Rodrigues
2. Nikola Canigova
3. Mario J. Avellaneda
4. Florian Gaertner
5. Kari Vaahtomeri
6. Michael Riedl
7. Jack Merrin
8. Robert Hauschild
9. Yoshinori Fukui
10. Alba Juanes Garcia
11. Michael Sixt

Reviewed by preLights

Pharmacologically inducing regenerative cardiac cells by small molecule drugs

1. Wei Zhou
2. Kezhang He
3. Chiyin Wang
4. Pengqi Wang
5. Dan Wang
6. Bowen Wang
7. Han Geng
8. Hong Lian
9. Tianhua Ma
10. Yu Nie
11. Sheng Ding

• Curated by eLife

  eLife Assessment

  This manuscript offers valuable information on the combinatory effect of small molecules, CHIR99021 and A-485 (2C), during the reprogramming of mature cardiomyocytes into regenerative cardiac cells on stimulating cardiac cell regeneration. Although the study used several hESC lines and an in vivo model of myocardial injury to demonstrate the regenerative potential of cardiac cells, the manuscript is still incomplete as several concerns remain unanswered, including the lack of validation of the conclusions from scRNA-seq. It is still unclear how a small fraction of dedifferentiating cardiac cells can offer such broad effects on regeneration both in vitro and in vivo. If validated, this study might unlock potential therapeutic strategies for cardiac regeneration.

Reviewed by eLife