Cell Biology

A green lifetime biosensor for calcium that remains bright over its full dynamic range

1. Franka H van der Linden
2. Stephen C Thornquist
3. Rick M ter Beek
4. Jelle Y Huijts
5. Mark A Hink
6. Theodorus WJ Gadella
7. Gaby Maimon
8. Joachim Goedhart

• Curated by eLife

  eLife Assessment

  This manuscript reports on an FLIM-based calcium biosensor, G-CaFLITS. It represents an important contribution to the field of genetically-encoded fluorescent biosensors, and will serve as a practical tool for the FLIM imaging community. The paper provides convincing evidence of G-CaFLITS's photophysical properties and its advantages over previous biosensors such as Tq-Ca-FLITS. Although the benefits of G-Ca-FLITS over Tq-Ca-FLITS are limited by the relatively small wavelength shift, it presents some advantages in terms of compatibility with available instrumentation and brightness consistency.

Reviewed by eLife

p53 engagement is a hallmark of an unfolded protein response in the nucleus of mammalian cells

1. Joseph H Park
2. Thomas J Wandless

• Curated by eLife

  eLife Assessment

  This paper presents useful findings that misfolded proteins in the nucleus can impair proteasomal degradation and activate p53. The results supporting the findings are largely solid, but incomplete. The manuscript could be strengthened by including more quantitative data analyses and additional experimentation/discussions on the mechanism of p53 activation by misfolded nuclear proteins. The work will be interesting primarily to scientists studying protein homeostasis.

Reviewed by eLife

Adenomatous polyposis coli (APC) regulates internalization and signaling of the chemorepellent receptor, Roundabout (ROBO) 1

1. Yi-Wei Huang
2. Jonathan St-Germain
3. Bo Wen Pang
4. Richard F Collins
5. Etienne Coyaud
6. Wenjuan Li
7. Amir Mohamed
8. Brian Raught
9. Sergio Grinstein
10. Lisa A Robinson

Reviewed by Review Commons

Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization

1. William D. Leineweber
2. Gabriela Acevedo Munares
3. Christian Leycam
4. Raul Michael
5. Juliette Noyer
6. Patrick Jurney

Reviewed by Arcadia Science

Restoring mechanophenotype reverts malignant properties of ECM-enriched vocal fold cancer

1. Jasmin Kaivola
2. Karolina Punovuori
3. Megan R. Chastney
4. Yekaterina A. Miroshnikova
5. Hind Abdo
6. Fabien Bertillot
7. Fabian Krautgasser
8. Jasmin Di Franco
9. James R.W. Conway
10. Gautier Follain
11. Jaana Hagström
12. Antti Mäkitie
13. Heikki Irjala
14. Sami Ventelä
15. Hellyeh Hamidi
16. Giorgio Scita
17. Roberto Cerbino
18. Sara A. Wickström
19. Johanna Ivaska

Reviewed by preLights

A FUCCI sensor reveals complex cell cycle organization of Toxoplasma endodyogeny

1. Mrinalini Batra
2. Clem Marsilia
3. Danya Awshah
4. Lauren M. Hawkins
5. Chengqi Wang
6. Dale Chaput
7. Daria A. Naumova
8. Elena S. Suvorova

Reviewed by Review Commons

Defective Neurogenesis in Lowe Syndrome is Caused by Mitochondria Loss and Cilia-related Sonic Hedgehog Defects

1. Chien-Hui Lo
2. Siyu Chen
3. Jingyu Zhao
4. Zhiquan Liu
5. Biao Wang
6. Qing Wang
7. Tia J Kowal
8. Yang Sun

• Curated by eLife

  eLife Assessment

  This study investigated mitochondrial dysfunction and the impairment of the ciliary Sonic Hedgehog signaling in Lowe syndrome (LS), a timely topic given the limited research in this area. The data from patient iPSC-derived neurons and a mouse model were collected using solid methods, but the evidence supporting key claims is incomplete, and some technical aspects fall short of expectations. Despite these limitations, the study provides a useful foundation for exploring the relationship between mitochondrial defects and primary cilia in neural development.

Reviewed by eLife

Vesiculation pathways in clathrin-mediated endocytosis

1. Xinran Wang
2. Julien Berro
3. Rui Ma

• Curated by eLife

  eLife Assessment

  This valuable study proposes a theoretical model of clathrin coat formation based on membrane elasticity that seeks to determine whether this process occurs by increasing the area of a protein-coated patch with constant curvature, or by increasing the curvature of a protein-coated patch that forms in an initially flat conformation (so called constant curvature or constant area models). Identifying energetically favorable pathways and comparing the obtained shapes with experiments provides solid support to the constant-area pathway. This work will be of interest for biologists and biophysicists interested in membrane remodelling and endocytosis. It provides an innovative approach to tackle the question of constant curvature vs. constant area coat protein formation, although some of the model's assumption are only partially supported by experimental evidence.

Reviewed by eLife

Chemotherapy resistance due to epithelial-to-mesenchymal transition is caused by abnormal lipid metabolic balance

1. Atsushi Matsumoto
2. Akihito Inoko
3. Waki Hosoda
4. Takahiro Kojima
5. Koji Ohnishi
6. Junichi Ikenouchi

• Curated by eLife

  eLife Assessment

  This paper presents the important discovery that lipid metabolic imbalance caused by Snail, an EMT-related transcription factor, contributes to the acquisition of chemoresistance in cancer cells. However, the incomplete support for the authors' claims is due to concerns about the causal relationship and lack of sufficient quantitative analysis. With strengthened evidence, this work would be of broad interest to researchers in the fields of cancer biology, lipid metabolism, and cell biology.

Reviewed by eLife

Endocytic recycling is central to circadian collagen fibrillogenesis and disrupted in fibrosis

1. Joan Chang
2. Adam Pickard
3. Jeremy A Herrera
4. Sarah O'Keefe
5. Richa Garva
6. Matthew Hartshorn
7. Anna Hoyle
8. Lewis Dingle
9. John Knox
10. Thomas A Jowitt
11. Madeleine Coy
12. Jason Wong
13. Adam Reid
14. Yinhui Lu
15. Cédric Zeltz
16. Rajamiyer V Venkateswaran
17. Patrick T Caswell
18. Stephen High
19. Donald Gullberg
20. Karl E Kadler

• Curated by eLife

  eLife Assessment

  This study describes a novel mechanism for how collagen fibrils are formed. The authors present compelling evidence that collagen-I fibrillogenesis relies on a functional endocytic system for recycling collagen-I, with circadian-regulated VPS33b and integrin-α11 being critical for fibril assembly. This is an important study for the understanding of the pathophysiology of collagen fibrillogenesis.

Reviewed by eLife