Cell Biology

Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii

1. Ron Kelley
2. Sagar Khavnekar
3. Ricardo D. Righetto
4. Jessica Heebner
5. Martin Obr
6. Xianjun Zhang
7. Saikat Chakraborty
8. Grigory Tagiltsev
9. Alicia K. Michael
10. Sofie van Dorst
11. Florent Waltz
12. Caitlyn L. McCafferty
13. Lorenz Lamm
14. Simon Zufferey
15. Philippe Van der Stappen
16. Hugo van den Hoek
17. Wojciech Wietrzynski
18. Pavol Harar
19. William Wan
20. John A.G. Briggs
21. Jürgen M. Plitzko
22. Benjamin D. Engel
23. Abhay Kotecha

Reviewed by Arcadia Science

The eduWOSM: a benchtop advanced microscope for education and research

1. Nicholas J Carter
2. Douglas S Martin
3. Justin E Molloy
4. Robert A Cross

• Curated by eLife

  eLife Assessment

  This is a valuable study and a promising development for the field of open-source microscopy for educational purposes. The strengths include the low cost of constructing the microscope, impressive performance and detailed resources including a dedicated website and YouTube channel. The claims are generally supported by solid evidence, however, the manuscript would be strengthened by inclusion of further details on standard performance metrics (e.g. signal to noise ratio etc.) compared to existing systems and further details and clarification on the microscope, construction and operation.

Reviewed by eLife

A green lifetime biosensor for calcium that remains bright over its full dynamic range

1. Franka H van der Linden
2. Stephen C Thornquist
3. Rick M ter Beek
4. Jelle Y Huijts
5. Mark A Hink
6. Theodorus WJ Gadella
7. Gaby Maimon
8. Joachim Goedhart

• Curated by eLife

  eLife Assessment

  This manuscript reports on an FLIM-based calcium biosensor, G-CaFLITS. It represents an important contribution to the field of genetically-encoded fluorescent biosensors, and will serve as a practical tool for the FLIM imaging community. The paper provides convincing evidence of G-CaFLITS's photophysical properties and its advantages over previous biosensors such as Tq-Ca-FLITS. Although the benefits of G-Ca-FLITS over Tq-Ca-FLITS are limited by the relatively small wavelength shift, it presents some advantages in terms of compatibility with available instrumentation and brightness consistency.

Reviewed by eLife

p53 engagement is a hallmark of an unfolded protein response in the nucleus of mammalian cells

1. Joseph H Park
2. Thomas J Wandless

• Curated by eLife

  eLife Assessment

  This paper presents useful findings that misfolded proteins in the nucleus can impair proteasomal degradation and activate p53. The results supporting the findings are largely solid, but incomplete. The manuscript could be strengthened by including more quantitative data analyses and additional experimentation/discussions on the mechanism of p53 activation by misfolded nuclear proteins. The work will be interesting primarily to scientists studying protein homeostasis.

Reviewed by eLife

Adenomatous polyposis coli (APC) regulates internalization and signaling of the chemorepellent receptor, Roundabout (ROBO) 1

1. Yi-Wei Huang
2. Jonathan St-Germain
3. Bo Wen Pang
4. Richard F Collins
5. Etienne Coyaud
6. Wenjuan Li
7. Amir Mohamed
8. Brian Raught
9. Sergio Grinstein
10. Lisa A Robinson

Reviewed by Review Commons

Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization

1. William D. Leineweber
2. Gabriela Acevedo Munares
3. Christian Leycam
4. Raul Michael
5. Juliette Noyer
6. Patrick Jurney

Reviewed by Arcadia Science

Restoring mechanophenotype reverts malignant properties of ECM-enriched vocal fold cancer

1. Jasmin Kaivola
2. Karolina Punovuori
3. Megan R. Chastney
4. Yekaterina A. Miroshnikova
5. Hind Abdo
6. Fabien Bertillot
7. Fabian Krautgasser
8. Jasmin Di Franco
9. James R.W. Conway
10. Gautier Follain
11. Jaana Hagström
12. Antti Mäkitie
13. Heikki Irjala
14. Sami Ventelä
15. Hellyeh Hamidi
16. Giorgio Scita
17. Roberto Cerbino
18. Sara A. Wickström
19. Johanna Ivaska

Reviewed by preLights

A FUCCI sensor reveals complex cell cycle organization of Toxoplasma endodyogeny

1. Mrinalini Batra
2. Clem Marsilia
3. Danya Awshah
4. Lauren M. Hawkins
5. Chengqi Wang
6. Dale Chaput
7. Daria A. Naumova
8. Elena S. Suvorova

Reviewed by Review Commons

Defective Neurogenesis in Lowe Syndrome is Caused by Mitochondria Loss and Cilia-related Sonic Hedgehog Defects

1. Chien-Hui Lo
2. Siyu Chen
3. Jingyu Zhao
4. Zhiquan Liu
5. Biao Wang
6. Qing Wang
7. Tia J Kowal
8. Yang Sun

• Curated by eLife

  eLife Assessment

  This study investigated mitochondrial dysfunction and the impairment of the ciliary Sonic Hedgehog signaling in Lowe syndrome (LS), a timely topic given the limited research in this area. The data from patient iPSC-derived neurons and a mouse model were collected using solid methods, but the evidence supporting key claims is incomplete, and some technical aspects fall short of expectations. Despite these limitations, the study provides a useful foundation for exploring the relationship between mitochondrial defects and primary cilia in neural development.

Reviewed by eLife

Vesiculation pathways in clathrin-mediated endocytosis

1. Xinran Wang
2. Julien Berro
3. Rui Ma

• Curated by eLife

  eLife Assessment

  This valuable study proposes a theoretical model of clathrin coat formation based on membrane elasticity that seeks to determine whether this process occurs by increasing the area of a protein-coated patch with constant curvature, or by increasing the curvature of a protein-coated patch that forms in an initially flat conformation (so called constant curvature or constant area models). Identifying energetically favorable pathways and comparing the obtained shapes with experiments provides solid support to the constant-area pathway. This work will be of interest for biologists and biophysicists interested in membrane remodelling and endocytosis. It provides an innovative approach to tackle the question of constant curvature vs. constant area coat protein formation, although some of the model's assumption are only partially supported by experimental evidence.

Reviewed by eLife