Cell Biology

Structural basis of respiratory complexes adaptation to cold temperatures

1. Young-Cheul Shin
2. Pedro Latorre-Muro
3. Amina Djurabekova
4. Oleksii Zdorevskyi
5. Christopher F. Bennett
6. Nils Burger
7. Kangkang Song
8. Chen Xu
9. Vivek Sharma
10. Maofu Liao
11. Pere Puigserver

Reviewed by preLights

Nitrogen availability and TOR signalling are important for preventing catastrophic mitosis in fission yeast

1. Viacheslav Zemlianski
2. Anna Marešová
3. Jarmila Princová
4. Roman Holič
5. Robert Häsler
6. Manuel José Ramos del Río
7. Laurane Lhoste
8. Maryia Zarechyntsava
9. Martin Převorovský

Reviewed by Review Commons

Neuropilin-1 controls vascular permeability through juxtacrine regulation of endothelial adherens junctions

1. Sagnik Pal
2. Yangyang Su
3. Lena Claesson-Welsh
4. Mark Richards

• Curated by eLife

  eLife assessment

  This study is focused on the question of how Nrp1 contributes to the regulation of vascular permeability and whether or why there are differences between different vascular beds. The scientific concept of this paper suggests a possible role of Nrp1 on perivascular cells as a participant in the regulation of vascular permeability. This concept is interesting and potentially useful. However, the methodology and quantitative analysis are currently inadequate to fully support the claims.

Reviewed by eLife

Senescence of endplate osteoclasts induces sensory innervation and spinal pain

1. Dayu Pan
2. Kheiria Gamal Benkato
3. Xuequan Han
4. Jinjian Zheng
5. Vijay Kumar
6. Mei Wan
7. Junying Zheng
8. Xu Cao

• Curated by eLife

  eLife assessment

  This fundamental study advances our understanding of the role of senescent osteoclasts (SnOCs) in the pathogenesis of spine instability. The authors provide compelling evidence for the SnOCs to induce sensory nerve innervation. Accordingly, reduction of SnOCs by the senolytic drug Navitoclax markedly reduces spinal pain sensitivity. This work will be of broad interest to regenerative biologists working on spinal pain.

Reviewed by eLife

Syntaxin 17 recruitment to mature autophagosomes is temporally regulated by PI4P accumulation

1. Saori Shinoda
2. Yuji Sakai
3. Takahide Matsui
4. Masaaki Uematsu
5. Ikuko Koyama-Honda
6. Jun-ichi Sakamaki
7. Hayashi Yamamoto
8. Noboru Mizushima

• Curated by eLife

  eLife assessment

  This paper addresses a fundamental issue in the field of autophagy: how is a protein responsible for autophagosome-lysosome fusion recruited to mature autophagosomes but not immature ones? The work succeeds in its ambition to provide a new conceptual advance. The evidence supporting the conclusions is convincing, with fluorescence microscopy, biochemical assays, and molecular dynamics simulations. This work will be of broad interest to cell biologists and biochemists studying autophagy, and also those focusing on lipid/membrane biology.

Reviewed by eLife

Retinal metabolism displays evidence for uncoupling of glycolysis and oxidative phosphorylation via Cori-, Cahill-, and mini-Krebs-cycle

1. Yiyi Chen
2. Laimdota Zizmare
3. Victor Calbiague
4. Lan Wang
5. Shirley Yu
6. Fritz W Herberg
7. Oliver Schmachtenberg
8. Francois Paquet-Durand
9. Christoph Trautwein

• Curated by eLife

  eLife assessment

  Chen and colleagues utilize an in situ explant model of the neural retina and retinal pigment epithelium (RPE), along with small molecule inhibition of key metabolic enzymes and targeted metabolomic analysis, to decipher key differences in metabolic pathways used by rods, cones, Muller glia, and the RPE. They conclude that rods are heavily reliant on oxidative metabolism, cones are heavily reliant on glycolysis, and multiple mechanisms exist to decouple glycolysis from oxidative metabolism in the retina. This study provides valuable metabolomic data and insights into the metabolic flexibility of different retinal cells. However, current evidence is still incomplete as several of the conclusions from the paper stand in contradiction to other published findings and the authors naturally suggests experiments that will be needed in the future to validate the hypothesized pathways and refute existing published data. Such future validation includes animal models with tissue specific knockout of the key enzymes probed in the study; inhibiting the targets of this study with more than 1 small molecule that is structurally different, and at different doses and timings; using retinal explants from matured animals; performing labeled metabolite tracing experiments; and direct assessment of mitochondrial function (via OCR) under various manipulations.

Reviewed by eLife

Plasmodium RON11 triggers biogenesis of the merozoite rhoptry pair and is essential for erythrocyte invasion

1. David Anaguano
2. Opeoluwa Adewale-Fasoro
3. Grace S. Vick
4. Sean Yanik
5. James Blauwkamp
6. Manuel A. Fierro
7. Sabrina Absalon
8. Prakash Srinivasan
9. Vasant Muralidharan

Reviewed by preLights

Tgif1-deficiency impairs cytoskeletal architecture in osteoblasts by activating PAK3 signaling

1. Simona Bolamperti
2. Hiroaki Saito
3. Sarah Heerdmann
4. Eric Hesse
5. Hanna Taipaleenmäki

• Curated by eLife

  eLife assessment

  This important work substantially advances our understanding of osteoblast migration to the sites of bone formation and regeneration. The evidence supporting the conclusion is compelling, with rigorous in vitro assays for cellular and biochemical aspects and with appropriate in vivo models. The work will be of broad interest to developmental biologists and bone biologists.

Reviewed by eLife

Pigmentation level of human iPSC-derived RPE does not indicate a specific gene expression profile

1. Yoko Nakai-Futatsugi
2. Jianshi Jin
3. Taisaku Ogawa
4. Noriko Sakai
5. Akiko Maeda
6. Ken-ichi Hironaka
7. Masakazu Fukuda
8. Hiroki Danno
9. Yuji Tanaka
10. Seiji Hori
11. Katsuyuki Shiroguchi
12. Masayo Takahashi

• Curated by eLife

  eLife assessment

  This useful work describes a novel microscopy-based method to correlate the degree of pigmentation with the gene expression profile of human-induced pluripotent stem cell-derived Retinal Pigmented Epithelial (iPSC-RPE) cells at the single cell level. The presented evidence is solid in showing that there is heterogeneous gene expression in iPSC-derived RPE cells, and there is no significant correlation with the pigmentation. By analyzing the expression of some genes related to function, lysosomal- and complement-related pathways were partially enriched in darker cells. This methodology can be used by other researchers interested in analyzing gene expression related to microscopic images.

Reviewed by eLife

The doublecortin-family kinase ZYG-8 ^DCLK1 regulates motor activity to achieve proper force balance in C. elegans acentrosomal spindles

1. Emily R. Czajkowski
2. Nikita S. Divekar
3. Sarah M. Wignall

Reviewed by Review Commons