Cell Biology

CILP from Cartilage Intermediate Zone Inhibits Hyaline Cartilage Fibrosis and Chondrocyte Ferroptosis via Keap1-Nrf2 Axis in Early Osteoarthritis Exercise Therapy

1. Shuangshuo Jia
2. Zhehan Hu
3. Zihan Li
4. Weiming Zhang
5. Liang Chen
6. Changping Niu
7. Ziqi Zhao
8. Yuhan Sun
9. Gang Yao
10. Yang Wang
11. Yue Yang

• Curated by eLife

  eLife Assessment

  This valuable manuscript by Jia et al. investigates the role of cartilage intermediate layer protein (CILP) and moderate exercise in maintaining hyaline cartilage integrity following anterior cruciate ligament transection (ACLt) in rats. Solid data support the downregulation of CILP in human OA cartilage and its potential role in regulating Keap1/Nrf2 interaction and chondrocyte ferroptosis. However, the data supporting a role for CILP in exercise-mediated inhibition of hyaline cartilage fibrosis in early OA are incomplete.

Reviewed by eLife

SARS-CoV-2 remodels the Golgi apparatus to facilitate viral assembly and secretion

1. Jianchao Zhang
2. Andrew Kennedy
3. Daniel Macedo de Melo Jorge
4. Lijuan Xing
5. Whitney Reid
6. Sarah Bui
7. Joseph Joppich
8. Molly Rose
9. Sevval Ercan
10. Qiyi Tang
11. Andrew W. Tai
12. Yanzhuang Wang

Reviewed by Review Commons, ScreenIT

Functional Impact of CYFIP2 RNA Editing on Actin Regulation, Axon Growth, and Spinogenesis

1. Luca La Via
2. Elona Ndoj
3. Matteo Bertoli
4. Veronica Mutti
5. Giulia Carini
6. Alice Filippini
7. Federica Bono
8. Chiara Fiorentini
9. Giovanni Ribaudo
10. Alessandra Gianoncelli
11. Giuseppe Borsani
12. Isabella Russo
13. Alessandro Barbon

Reviewed by Arcadia Science

SAM transmethylation pathway and adenosine recycling to ATP are essential for systemic regulation and immune response

1. Pavla Nedbalová
2. Nikola Kaislerova
3. Lenka Chodakova
4. Martin Moos
5. Tomáš Doležal

• Curated by eLife

  eLife Assessment

  This paper provides a valuable contribution to our understanding of how adenosine acts as a signal of nutrient insufficiency and extends this idea to suggest that adenosine is released by metabolically active cells in proportion to the activity of methylation events. Convincing data supports this idea. The authors use metabolic tracing approaches to identify the biochemical pathways that contribute to the regulation of adenosine levels and the S-adenosylmethionine cycle in Drosophila larval hemocytes in response to wasp egg infection.

Reviewed by eLife

Human CD29+/CD56+ myogenic progenitors display tenogenic differentiation potential and facilitate tendon regeneration

1. Xiexiang Shao
2. Xingzuan Lin
3. Hao Zhou
4. Minghui Wang
5. Lili Han
6. Xin Fu
7. Sheng Li
8. Siyuan Zhu
9. Shenao Zhou
10. Wenjun Yang
11. Jianhua Wang
12. Zhanghua Li
13. Ping Hu

• Curated by eLife

  eLife Assessment

  The authors demonstrate the valuable discovery that human CD29+/CD56+ myogenic progenitors can differentiate into tendon through the TGFβ pathway, addressing mouse and human interspecies differences in regard to the potential of muscle stem cells. The in vivo transplantation experiments provide convincing evidence for the conclusion, as human CD29+/CD56+ myogenic progenitors contribute to tendon regeneration, resulting in functional recovery in mouse model. The authors' approach can be used for the development of cell therapy for tendon-injured patients.

Reviewed by eLife

Local nuclear to cytoplasmic ratio regulates H3.3 incorporation via cell cycle state during zygotic genome activation

1. Anusha D. Bhatt
2. Madeleine G. Brown
3. Aurora B. Wackford
4. Yuki Shindo
5. Amanda A. Amodeo

Reviewed by Review Commons

Hydroxyurea induces ER stress and cytoplasmic protein aggregation

1. Ana Sánchez-Molina
2. Manuel Bernal
3. Joel D. Posligua-García
4. Antonio J. Pérez-Pulido
5. Laura de Cubas
6. Elena Hidalgo
7. Silvia Salas-Pino
8. Rafael R. Daga

Reviewed by Review Commons

Cell crowding activates pro-invasive mechanotransduction pathway in high-grade DCIS via TRPV4 inhibition and cell volume reduction

1. Xiangning Bu
2. Nathanael Ashby
3. Teresa Vitali
4. Sulgi Lee
5. Ananya Gottumukkala
6. Kangsun Yun
7. Sana Tabbara
8. Patricia Latham
9. Christine Teal
10. Inhee Chung

• Curated by eLife

  eLife Assessment

  This fundamental study provides compelling evidence that TRPV4 plays a crucial role in mechanical sensing during cancer cell transition from non-invasive to invasive states, and offers novel insights into metastasis. By employing multiple experimental approaches, including pharmacological and genetic manipulation, as well as advanced imaging techniques, the authors demonstrate a strong correlation between TRPV4 dynamics, calcium homeostasis, and cell volume plasticity. The findings significantly enhance our understanding of mechanotransduction in cancer and present TRPV4 as a promising therapeutic target for inhibiting metastasis.

Reviewed by eLife

Subcellular Region Morphology Reflects Cellular Identity

1. Ángel-Carlos Román
2. Alba Diaz-Pizarro
3. Nuria Del Valle-Del Pino
4. Marcos Olivera-Gómez
5. Guadalupe Cumplido-Laso
6. Dixan Agustín Benítez
7. Jose María Carvajal-González
8. Sonia Mulero-Navarro

Reviewed by Review Commons

Stratification of enterochromaffin cells by single-cell expression analysis

1. Yan Song
2. Linda J Fothergill
3. Kari S Lee
4. Brandon Y Liu
5. Ada Koo
6. Mark Perelis
7. Shanti Diwakarla
8. Brid Callaghan
9. Jie Huang
10. Jill Wykosky
11. John B Furness
12. Gene W Yeo

• Curated by eLife

  eLife Assessment

  This important study presents a transcriptomic analysis of enterochromaffin cells in the intestine. The evidence supporting the authors' claims is solid, although the functional analysis is focused on the Piezo2-expressing subset in the colon. The work will be of interest to biologists working on intestinal mucosal biology.

Reviewed by eLife