Cell Biology

Profiling the physiological impact of aberrant folded-state protein filamentation in cells

1. Tal Levin
2. Hector Garcia-Seisdedos
3. Arseniy Lobov
4. Matthias Wojtynek
5. Alexander Alexandrov
6. Ghil Jona
7. Dikla Levi
8. Ohad Medalia
9. D. Emmanuel Levy

Reviewed by Review Commons

N-cadherin mechanosensing in ovarian follicles controls oocyte maturation and ovulation

1. Alaknanda Emery
2. Orest W Blaschuk
3. Doan T Dinh
4. Tim McPhee
5. Rouven Becker
6. Andrew D Abell
7. Krzysztof M Mrozik
8. Andrew CW Zannettino
9. Rebecca L Robker
10. Darryl L Russell

• Curated by eLife

  eLife assessment

  The manuscript describes important findings regarding the significance of CHD2 in ovarian folliculogenesis. Overall, the results lead to convincing conclusions, with minimal concerns raised by the reviewers. Both the results and conclusions are well discussed. This work will be of interest to ovarian biologists and physicians working on female fertility.

Reviewed by eLife

Kinesin-2 autoinhibition requires elbow phosphorylation

1. Guanghan Chen
2. Zhengyang Guo
3. Zhiwen Zhu
4. Shanshan Xie
5. Tianhua Zhou
6. Guangshuo Ou

• Curated by eLife

  eLife Assessment

  In their important manuscript, Chen et al. investigate the phospho-regulation of the C. elegans kinesin-2 motor protein OSM-3, revealing that the kinase, NEKL-3, phosphorylates a serine/threonine patch at the hinge region of the motor to mediate autoinhibition until it reaches the ciliary middle segment. The findings are supported by robust genetic data, in vivo imaging, and motility assays with wild-type and mutant motors. Overall, the study provides a compelling contribution to understanding the regulation of OSM-3 kinesin activity both on the molecular and cellular levels.

Reviewed by eLife

A novel approach to tagging tubulin reveals MT assembly dynamics of the axoneme in Trypanosoma brucei

1. Daniel Abbühl
2. Martina Pružincová
3. Luděk Štěpánek
4. Eleonore Bouscasse
5. Rita Azevedo
6. Mariette Matondo
7. Vladimir Varga
8. Serge Bonnefoy
9. Philippe Bastin

Reviewed by Review Commons

Image Correlation Spectroscopy is a Robust Tool to Quantify Cellular DNA Damage Response

1. Angelica A Gopal
2. Bianca Fernandez
3. Paul W Wiseman
4. J Matthew Dubach

• Curated by eLife

  eLife Assessment

  This valuable paper shows image correlation spectroscopy (ICS) as a new tool to analyze the clustering of proteins involved in DNA damage response (DDR). The convincing evidence presented demonstrates that ICS is more sensitive than traditional foci counting. This new method provides an alternative tool to quantify immunostained foci for researchers in the fields of DDR and cell biology.

Reviewed by eLife

p66Shc Mediates SUMO2-induced Endothelial Dysfunction

1. Jitendra Kumar
2. Shravan K Uppulapu
3. Sujata Kumari
4. Kanika Sharma
5. William Paradee
6. Ravi Prakash Yadav
7. Vikas Kumar
8. Santosh Kumar

• Curated by eLife

  eLife Assessment

  This study offers valuable insights into the role of post-translational modifiers, specifically SUMO2ylation at K81 in p66Shc, and its impact on endothelial function through reactive oxygen species. A series of compelling experiments demonstrated that lysine 81 of p66Shc is the site of SUMO2 conjugation, which is crucial for mitochondrial localization and essential for S36 phosphorylation, leading to specific pathological effects. The combination of cell overexpression and animal studies provides solid data supporting this mechanistic link.

Reviewed by eLife

Mutations that prevent phosphorylation of the BMP4 prodomain impair proteolytic maturation of homodimers leading to lethality in mice

1. Hyung-Seok Kim
2. Mary L Sanchez
3. Joshua Silva
4. Heidi L Schubert
5. Rebecca Dennis
6. Christopher P Hill
7. Jan L Christian

• Curated by eLife

  eLife Assessment

  This fundamental work presents two clinically relevant BMP4 mutations that contribute to vertebrate development. The compelling evidence, both from wet lab and AI generated predictions, supports that the site-specific cleavage at the BMP4 pro-domain precisely regulates its function and provides mechanistic insight how homodimers and heterodimers behave differently. The work will be of broad interest to researchers working on growth factor signaling mechanisms and vertebrate development.

Reviewed by eLife

Monocyte-derived macrophage recruitment mediated by TRPV1 is required for eardrum wound healing

1. Yunpei Zhang
2. Pingting Wang
3. Lingling Neng
4. Kushal Sharma
5. Allan Kachelmeier
6. Xiaorui Shi

• Curated by eLife

  eLife Assessment

  This study exploring the role of TRPV1 signaling in recruiting macrophages and promoting angiogenesis during tympanic membrane wound healing presents useful findings. However, the strength of evidence supporting the central claims is incomplete, as the mechanistic links between TRPV1 activation and immune cell recruitment remain largely correlative and rely heavily on previously published datasets without sufficient functional validation. The work will be of interest to researchers studying wound healing and sensory-immune interactions, though substantial revisions are needed to support its broader significance.

Reviewed by eLife

Dual regulation of the unfolded protein response by IGF2BP3 during ER stress

1. Aleksandra S Anisimova
2. Harald Hornegger
3. Irmgard Fischer
4. Gijs A Versteeg
5. Stefan L Ameres
6. G Elif Karagöz

Reviewed by PREreview

Random UV Mutagenesis for the production of Chlorella vulgaris mutants with low chlorophyll content for the food industry

1. Ivan N. Ivanov
2. Jiří Kopecký
3. Karolína Štěrbová
4. Pavel Hrouzek
5. Martin Lukeš
6. Kateřina Bišová
7. Setyo Budi Kurniawan

Reviewed by Arcadia Science