Cell Biology

Pharmacologic activation of integrated stress response kinases inhibits pathologic mitochondrial fragmentation

1. Kelsey R Baron
2. Samantha Oviedo
3. Sophia Krasny
4. Mashiat Zaman
5. Rama Aldakhlallah
6. Prerona Bora
7. Prakhyat Mathur
8. Gerald Pfeffer
9. Michael J Bollong
10. Timothy E Shutt
11. Danielle A Grotjahn
12. R Luke Wiseman

• Curated by eLife

  eLife Assessment

  This important study identifies a new class of small molecules that activate the integrated stress response (ISR) via the kinase HRI. Convincing evidence, including the image analysis pipeline, indicates that two of these compounds promote mitochondrial elongation and protect against mitochondrial fragmentation caused by chemical stress conditions or by genetic alterations. These findings open an avenue for new strategies for mitochondrial dysfunction targeting linked to ISR alterations.

Reviewed by eLife

Catalytic growth in a shared enzyme pool ensures robust control of centrosome size

1. Deb Sankar Banerjee
2. Shiladitya Banerjee

• Curated by eLife

  eLife Assessment

  This valuable work suggests a new physical model of centrosome maturation: a catalytic growth model with a shared enzyme pool. The authors provide compelling evidence to show that the model is able to reproduce various experimental results such as centrosome size scaling with cell size and centrosome growth curves in C. elegans, and that the final centrosome size is more robust to differences in initial centrosome size. While direct experimental support for this theory is currently lacking, the authors propose concrete experiments that could distinguish their shared-enzyme model from previously proposed alternatives.

Reviewed by eLife

Chromatin activity of IκBα mediates the exit from naïve pluripotency

1. Luis G. Palma
2. Daniel Álvarez-Villanueva
3. María Maqueda
4. Mercedes Barrero
5. Arnau Iglesias
6. Joan Bertran
7. Damiana Álvarez-Errico
8. Carlos A. García-Prieto
9. Cecilia Ballaré
10. Virginia Rodriguez-Cortez
11. Clara Bueno
12. August Vidal
13. Alberto Villanueva
14. Pablo Menéndez
15. Gregoire Stik
16. Luciano Di Croce
17. Bernhard Payer
18. Manel Esteller
19. Lluís Espinosa
20. Anna Bigas

• Curated by eLife

  eLife Assessment

  This important study reveals a role for IκBα in the regulation of embryonic stem cell pluripotency. The solid data in mouse embryonic stem cells include separation of function mutations in IκBα to dissect its non-canonical role as a chromatin regulator and its canonical function as NF-κB inhibitor. The conclusions could be strengthened by including better markers of differentiation status and additional controls or orthogonal approaches.

Reviewed by eLife

Lysine Demethylase 4A is a Centrosome Associated Protein Required for Centrosome Integrity and Genomic Stability

1. Pratim Chowdhury
2. Xiaoli Wang
3. Julia F. Love
4. Sofia Vargas-Hernandez
5. Yuya Nakatani
6. Sandra L. Grimm
7. Dereck Mezquita
8. Frank M. Mason
9. Elisabeth D. Martinez
10. Cristian Coarfa
11. Cheryl L. Walker
12. Anna-Karin Gustavsson
13. Ruhee Dere

Reviewed by Review Commons

A hierarchical pathway for assembly of the distal appendages that organize primary cilia

1. Tomoharu Kanie
2. Beibei Liu
3. Julia F Love
4. Saxton D Fisher
5. Anna-Karin Gustavsson
6. Peter K Jackson

• Curated by eLife

  eLife assessment

  This important and detailed study presents the most comprehensive view of the functional organization and requirements for a mother centriole's distal appendage in primary cilia assembly published to date. Crispr-knockouts and super-resolution microscopy analysis of the distal appendage proteins provides convincing evidence to support the claims of the authors. This work will be of high value to cell biologists and biophysicists working on the structure and function of the centrosome as well as human geneticists exploring ciliary pathology.

Reviewed by eLife

Necrosulfonamide causes oxidation of PCM1 and impairs ciliogenesis and autophagy

1. Clotilde C.N. Renaud
2. Carolina Alves Nicolau
3. Clément Maghe
4. Kilian Trillet
5. Jane Jardine
6. Sophie Escot
7. Nicolas David
8. Julie Gavard
9. Nicolas Bidère

Reviewed by preLights

AtVPS13M1 is involved in lipid remodeling in low phosphate and is located at the mitochondria surface in plants

1. Sébastien Leterme
2. Catherine Albrieux
3. Sabine Brugière
4. Yohann Couté
5. Julien Dellinger
6. Benjamin Gillet
7. Sandrine Hughes
8. Julie Castet
9. Amélie Bernard
10. David Scheuring
11. Marion Schilling
12. Juliette Jouhet
13. Morgane Michaud

Reviewed by Review Commons

Dissociation of the nuclear basket triggers chromosome loss in aging yeast

1. Mihailo Mirkovic
2. Jordan McCarthy
3. Anne Cornelis Meinema
4. Julie Parenteau
5. Sung Sik Lee
6. Sherif Abou Elela
7. Yves Barral

• Curated by eLife

  eLife Assessment

  This fundamental study reveals that aging in yeast leads to chromosome mis-segregation due to asymmetric partitioning of chromosomes, driven by disruption of the nuclear pore complex and pre-mRNA leakage. The findings are convincingly supported by carefully-designed experimental data with a combination of genetic, molecular biology and cell biology approaches.

Reviewed by eLife

Image-based profiling of healthy donor immune cells with Blood Cell Painting reveals novel genotype-phenotype associations

1. Caroline Högel-Starck
2. Veera A. Timonen
3. Gantugs Atarsaikhan
4. Isabel Mogollon
5. Minttu Polso
6. Antti Hassinen
7. Jarno Honkanen
8. Cindy David Sarmento
9. Jessica Koski
10. Julius Soini
11. Lea Urpa
12. Tanja Ruokoranta
13. Toveann Ahlnäs
14. Julianna Juvila
15. Juho J. Miettinen
16. Rodosthenis S. Rodosthenous
17. FinnGen
18. Outi Kilpivaara
19. Mikko Arvas
20. Caroline A. Heckman
21. Jukka Partanen
22. Mark Daly
23. Aarno Palotie
24. Hanna M. Ollila
25. Lassi Paavolainen
26. Vilja Pietiäinen
27. Esa Pitkänen

Reviewed by Arcadia Science

Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii

1. Ron Kelley
2. Sagar Khavnekar
3. Ricardo D. Righetto
4. Jessica Heebner
5. Martin Obr
6. Xianjun Zhang
7. Saikat Chakraborty
8. Grigory Tagiltsev
9. Alicia K. Michael
10. Sofie van Dorst
11. Florent Waltz
12. Caitlyn L. McCafferty
13. Lorenz Lamm
14. Simon Zufferey
15. Philippe Van der Stappen
16. Hugo van den Hoek
17. Wojciech Wietrzynski
18. Pavol Harar
19. William Wan
20. John A.G. Briggs
21. Jürgen M. Plitzko
22. Benjamin D. Engel
23. Abhay Kotecha

Reviewed by Arcadia Science