Cell Biology

SAM transmethylation pathway and adenosine recycling to ATP are essential for systemic regulation and immune response

1. Pavla Nedbalová
2. Nikola Kaislerova
3. Lenka Chodakova
4. Martin Moos
5. Tomáš Doležal

• Curated by eLife

  eLife Assessment

  This paper provides a valuable contribution to our understanding of how adenosine acts as a signal of nutrient insufficiency and extends this idea to suggest that adenosine is released by metabolically active cells in proportion to the activity of methylation events. Convincing data supports this idea. The authors use metabolic tracing approaches to identify the biochemical pathways that contribute to the regulation of adenosine levels and the S-adenosylmethionine cycle in Drosophila larval hemocytes in response to wasp egg infection.

Reviewed by eLife

Human CD29+/CD56+ myogenic progenitors display tenogenic differentiation potential and facilitate tendon regeneration

1. Xiexiang Shao
2. Xingzuan Lin
3. Hao Zhou
4. Minghui Wang
5. Lili Han
6. Xin Fu
7. Sheng Li
8. Siyuan Zhu
9. Shenao Zhou
10. Wenjun Yang
11. Jianhua Wang
12. Zhanghua Li
13. Ping Hu

• Curated by eLife

  eLife Assessment

  The authors demonstrate the valuable discovery that human CD29+/CD56+ myogenic progenitors can differentiate into tendon through the TGFβ pathway, addressing mouse and human interspecies differences in regard to the potential of muscle stem cells. The in vivo transplantation experiments provide convincing evidence for the conclusion, as human CD29+/CD56+ myogenic progenitors contribute to tendon regeneration, resulting in functional recovery in mouse model. The authors' approach can be used for the development of cell therapy for tendon-injured patients.

Reviewed by eLife

Local nuclear to cytoplasmic ratio regulates H3.3 incorporation via cell cycle state during zygotic genome activation

1. Anusha D. Bhatt
2. Madeleine G. Brown
3. Aurora B. Wackford
4. Yuki Shindo
5. Amanda A. Amodeo

Reviewed by Review Commons

Hydroxyurea induces ER stress and cytoplasmic protein aggregation

1. Ana Sánchez-Molina
2. Manuel Bernal
3. Joel D. Posligua-García
4. Antonio J. Pérez-Pulido
5. Laura de Cubas
6. Elena Hidalgo
7. Silvia Salas-Pino
8. Rafael R. Daga

Reviewed by Review Commons

Cell crowding activates pro-invasive mechanotransduction pathway in high-grade DCIS via TRPV4 inhibition and cell volume reduction

1. Xiangning Bu
2. Nathanael Ashby
3. Teresa Vitali
4. Sulgi Lee
5. Ananya Gottumukkala
6. Kangsun Yun
7. Sana Tabbara
8. Patricia Latham
9. Christine Teal
10. Inhee Chung

• Curated by eLife

  eLife Assessment

  This fundamental study provides compelling evidence that TRPV4 plays a crucial role in mechanical sensing during cancer cell transition from non-invasive to invasive states, and offers novel insights into metastasis. By employing multiple experimental approaches, including pharmacological and genetic manipulation, as well as advanced imaging techniques, the authors demonstrate a strong correlation between TRPV4 dynamics, calcium homeostasis, and cell volume plasticity. The findings significantly enhance our understanding of mechanotransduction in cancer and present TRPV4 as a promising therapeutic target for inhibiting metastasis.

Reviewed by eLife

Subcellular Region Morphology Reflects Cellular Identity

1. Ángel-Carlos Román
2. Alba Diaz-Pizarro
3. Nuria Del Valle-Del Pino
4. Marcos Olivera-Gómez
5. Guadalupe Cumplido-Laso
6. Dixan Agustín Benítez
7. Jose María Carvajal-González
8. Sonia Mulero-Navarro

Reviewed by Review Commons

Stratification of enterochromaffin cells by single-cell expression analysis

1. Yan Song
2. Linda J Fothergill
3. Kari S Lee
4. Brandon Y Liu
5. Ada Koo
6. Mark Perelis
7. Shanti Diwakarla
8. Brid Callaghan
9. Jie Huang
10. Jill Wykosky
11. John B Furness
12. Gene W Yeo

• Curated by eLife

  eLife Assessment

  This important study presents a transcriptomic analysis of enterochromaffin cells in the intestine. The evidence supporting the authors' claims is solid, although the functional analysis is focused on the Piezo2-expressing subset in the colon. The work will be of interest to biologists working on intestinal mucosal biology.

Reviewed by eLife

Capsaicin acts as a novel NRF2 agonist to suppress ethanol induced gastric mucosa oxidative damage by directly disrupting the KEAP1-NRF2 interaction

1. Xiaoning Gao
2. WuYan Guo
3. Peiyuan Liu
4. Mingyue Yuwen
5. Zixiang Liu
6. Ruyang Tan
7. Kairui Liu
8. Zhiru Yang
9. Junli Ba
10. Xue Bai
11. Shiti Shama
12. Cong Tang
13. Kai Miao
14. Haozhi Pei
15. Liren Liu
16. Cheng Zhu
17. Tao Wang
18. Bo Zhang
19. Jun Kang

• Curated by eLife

  eLife Assessment

  This valuable study suggests that capsaicin nanoparticle administration in rats activates the transcription factor Nrf2 by directly binding to its repressor, KEAP1, leading to the induction of cytoprotective genes and preventing alcohol-induced gastric damage, offering a potential avenue for treating alcoholism-related gastric disorders. Although improvements were made following the first revision, the evidence supporting capsaicin as an Nrf2 activator remains incomplete, as some methodological aspects still require revision and the interpretation of key data needs further clarification.

Reviewed by eLife

Cytosolic Carboxypeptidase 5 maintains mammalian ependymal multicilia to ensure proper homeostasis and functions of the brain

1. Rubina Dad
2. Yujuan Wang
3. Chuyu Fang
4. Yuncan Chen
5. Yuan Zhang
6. Xinwen Pan
7. Xinyue Zhang
8. Emily Swanekamp
9. Krish Patel
10. Matthias TF Wolf
11. Zhiguang Yuchi
12. Xueliang Zhu
13. Hui-Yuan Wu

• Curated by eLife

  eLife Assessment

  This is a valuable study that explores the function of CCP5 in mouse ependymal cells. The methods, data, and analyses broadly support the claims. However, the study is incomplete as it stands. Minor weaknesses remain and the authors may wish to address them.

Reviewed by eLife

Proteome dynamics reveal Leiomodin 1 as a key regulator of myogenic differentiation

1. Ellen Späth
2. Svenja C Schüler
3. Ivonne Heinze
4. Therese Dau
5. Alberto Minetti
6. Maleen Hofmann
7. Julia von Maltzahn
8. Alessandro Ori

• Curated by eLife

  eLife Assessment

  This valuable study offers insights into the role of Leiomodin-1 (LMOD1) in muscle stem cell biology, advancing our understanding of myogenic differentiation and indicating LMOD1 as a regulator of muscle regeneration, aging, and exercise adaptation. The integration of in vitro and in vivo approaches, complemented by proteomic and imaging methodologies, is solid. However, certain aspects require further attention to improve the clarity, impact, and overall significance of the work, particularly in substantiating the in vivo relevance. This work will provide a starting point that will be of value to medical biologists and biochemists working on LMOD and its variants in muscle biology.

Reviewed by eLife