Cell Biology

Spatially resolved rewiring of mitochondria-lipid droplet interactions in hepatic lipid homeostasis

1. Sun Woo Sophie Kang
2. Lauryn A Brown
3. Colin B Miller
4. Katherine M Barrows
5. Jihye L Golino
6. Constance M Cultraro
7. Daniel Feliciano
8. Mercedes B. Cornelius-Muwanuzi
9. Andy D Tran
10. Michael Kruhlak
11. Alexei Lobanov
12. Maggie Cam
13. Natalie Porat-Shliom

Reviewed by PREreview

Image-based identification and isolation of micronucleated cells to dissect cellular consequences

1. Lucian DiPeso
2. Sriram Pendyala
3. Heather Z Huang
4. Douglas M Fowler
5. Emily M Hatch

• Curated by eLife

  eLife Assessment

  This valuable paper reports machine learning-based image analysis pipelines for the automated segmentation of micronuclei and the detection and sorting of micronuclei-containing cells. These are powerful new tools for researchers who study micronuclei and their physiologic consequences. The analysis of the new tools and their benchmarking is rigorous and convincing; applications and remaining limitations are well explained in the paper.

Reviewed by eLife

Adventitial fibroblasts direct smooth muscle cell-state transition in pulmonary vascular disease

1. Slaven Crnkovic
2. Helene Thekkekara Puthenparampil
3. Shirin Mulch
4. Valentina Biasin
5. Nemanja Radic
6. Jochen Wilhelm
7. Marek Bartkuhn
8. Ehsan Bonyadi Rad
9. Alicja Wawrzen
10. Ingrid Matzer
11. Ankita Mitra
12. Ryan D Leib
13. Bence Miklos Nagy
14. Anita Sahu-Osen
15. Francesco Valzano
16. Natalie Bordag
17. Matthias Evermann
18. Konrad Hoetzenecker
19. Andrea Olschewski
20. Senka Ljubojevic-Holzer
21. Malgorzata Wygrecka
22. Kurt Stenmark
23. Leigh M Marsh
24. Vinicio de Jesus Perez
25. Grazyna Kwapiszewska

• Curated by eLife

  eLife Assessment

  This fundamental research conducted a molecular comparison between smooth muscle cells and adjacent fibroblast cells within lung blood vessels affected by pulmonary arterial hypertension. The study identified distinct disease-related states in each cell type and provided deeper insights into their interactions and communication. While certain conclusions should be interpreted with caution due to inherent methodological limitations, the study's findings remain convincing and robust. This is supported by the use of advanced and complementary techniques, as well as the rare isolation of diseased lung blood vessel cells from the same donor, enabling direct comparison.

Reviewed by eLife

Nuclear Argonaute protein NRDE-3 switches small RNA partners during embryogenesis to mediate temporal-specific gene regulatory activity

1. Shihui Chen
2. Carolyn Marie Phillips

• Curated by eLife

  eLife Assessment

  The study by Chen and Phillips provides evidence for a dynamic switch in the small RNA repertoire of the Argonaute protein NRDE-3 during embryogenesis in C. elegans. The work is supported by convincing experimental data, shedding light on RNA regulation during development. While the functional relevance of this process warrants further investigation, this study provides valuable insights into small RNA pathways with broader implications for developmental biology and gene regulation in other systems.

Reviewed by eLife

Single-nucleus transcriptomics reveal the cytological mechanism of conjugated linoleic acids in regulating intramuscular fat deposition

1. Liyi Wang
2. Shiqi Liu
3. Shu Zhang
4. Yizhen Wang
5. Yanbing Zhou
6. Tizhong Shan

• Curated by eLife

  eLife Assessment

  This study provides valuable information on the single nucleus RNA sequencing transcriptome, pathways, and cell types in pig skeletal muscle in response to conjugated linoleic acid (CLA) supplementation. Based on the comprehensive data analyses, the data are considered compelling and provide new insight into the mechanisms underlying intramuscular fat deposition and muscle fiber remodeling. The study contributes significantly to the understanding of nutritional strategies for fat infiltration in pig muscle.

Reviewed by eLife

Med12 cooperates with multiple differentiation signals to facilitate efficient lineage transitions in embryonic stem cells

1. Max Fernkorn
2. Christian Schröter

Reviewed by Review Commons

Piezo1 balances membrane tension and cortical contractility to stabilize intercellular junctions and maintain epithelial barrier integrity

1. Ahsan Javed
2. Aki Stubb
3. Clémentine Villeneuve
4. Franziska Peters
5. Matthias Rübsam
6. Carien M. Niessen
7. Leah C. Biggs
8. Sara A. Wickström

Reviewed by Review Commons

Clathrin-independent endocytosis and retrograde transport in cancer cells promote cytotoxic CD8 T cell activation

1. Shiqiang Xu
2. Alix Buridant
3. Thibault Hirsch
4. Massiullah Shafaq-Zadah
5. Estelle Dransart
6. Benjamin Ledoux
7. Ludger Johannes
8. Pierre van der Bruggen
9. Pierre Morsomme
10. Henri-François Renard

• Curated by eLife

  eLife Assessment

  This valuable study provides convincing evidence that specific proteins on the surface of cancer cells undergo a particular form of recycling and are redirected toward the cell-cell contact with T cells, a type of immune cell. However, the characterization of the consequences of T cell activation resulting from perturbing the recycling pathway is incomplete. Furthermore, relevant literature has not been sufficiently cited.

Reviewed by eLife

The RAB27A effector SYTL5 regulates mitophagy and mitochondrial metabolism

1. Ana Lapao
2. Lauren Sophie Johnson
3. Laura Trachsel-Moncho
4. Sakshi Singh
5. Matthew Yoke Wui Ng
6. Sigve Nakken
7. Eeva-Liisa Eskelinen
8. Anne Simonsen

• Curated by eLife

  eLife Assessment

  This study presents valuable insights into the role of two proteins, Rab27A and SYTL5, which control vesicle transport and delivery. While the data is clear, the overall evidence is somewhat incomplete. Strengthening the mechanistic aspect would enhance the study, making it of greater interest to cell biologists studying membrane trafficking and mitochondria.

Reviewed by eLife

Control of ciliary transcriptional programs during spermatogenesis by antagonistic transcription factors

1. Weihua Wang
2. Junqiao Xing
3. Xiqi Zhang
4. Hongni Liu
5. Xingyu Liu
6. Haochen Jiang
7. Cheng Xu
8. Xue Zhao
9. Zhangfeng Hu

• Curated by eLife

  eLife Assessment

  This valuable study presents data suggesting the critical roles of two ancient proteins, XAP5 and XAP5L, in regulating the transcriptional program of ciliogenesis during mouse spermatogenesis. The supporting data are solid, and this work will be of interest to biomedical researchers studying ciliogenesis and reproduction.

Reviewed by eLife