Cell Biology

A novel SUN1-ALLAN complex coordinates segregation of the bipartite MTOC across the nuclear envelope during rapid closed mitosis in Plasmodium berghei

1. Mohammad Zeeshan
2. Igor Blatov
3. Ryuji Yanase
4. David JP Ferguson
5. Sarah L Pashley
6. Zeinab Chahine
7. Yoshiki Yamaryo-Botté
8. Akancha Mishra
9. Baptiste Marche
10. Suhani Bhanvadia
11. Molly Hair
12. Sagar Batra
13. Robert Markus
14. Declan Brady
15. Andrew R Bottrill
16. Sue Vaughan
17. Cyrille Y Botté
18. Karine G Le Roch
19. Anthony A Holder
20. Eelco Tromer
21. Rita Tewari

• Curated by eLife

  eLife Assessment

  In Plasmodium male gametocytes, rapid nuclear division occurs with an intact nuclear envelope, requiring precise coordination between nuclear and cytoplasmic events to ensure proper packaging of each nucleus into a developing gamete. This valuable study characterizes two proteins involved in the formation of Plasmodium berghei male gametes. By integrating live-cell imaging, ultrastructural expansion microscopy, and proteomics, this study convincingly identifies SUN1 and its interaction partner ALLAN as crucial nuclear envelope components in male gametogenesis. A role for SUN1 in membrane dynamics and lipid metabolism is less well supported. The results are of interest for general cell biologists working on unusual mitosis pathways.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Heme’s relevance genuine? Re-visiting the roles of TANGO2 homologs including HRG-9 and HRG-10 in C. elegans

1. Sarah E Sandkuhler
2. Kayla S Youngs
3. Laura Owlett
4. Monica B Bandora
5. Aaliya Naaz
6. Euri S Kim
7. Lili Wang
8. Andrew P Wojtovich
9. Vandana A Gupta
10. Michael Sacher
11. Samuel J Mackenzie

• Curated by eLife

  eLife Assessment

  This useful study provides incomplete evidence that TANGO2 homologs, including HRG-9 and HRG-10, are not heme chaperones but play a role in cellular bioenergetics and oxidative stress homeostasis. While outstanding strengths include the use of different model systems, genetic tools, and behavioral assays, there are weaknesses in the data presented for the conclusions drawn. Due to the differences in experimental protocols between this study and the previous work reported by Sun et al., it is insufficient to rule out the role of TANGO2 as a heme chaperone, and furthermore, the authors provide only indirect evidence for the role of TANGO2 in bioenergetic and oxidative stress pathways. Nevertheless, this study paves the way for future mechanistic studies addressing the mechanisms of how TANGO2 regulates oxidative stress independent of its previously demonstrated role as a heme chaperone.

Reviewed by eLife

Chronic stress antagonizes formation of Stress Granules

1. Yuichiro Adachi
2. Allison M. Williams
3. Masashi Masuda
4. Yutaka Taketani
5. Paul J. Anderson
6. Pavel Ivanov

Reviewed by preLights

Functional redundancy and formin-independent localization of tropomyosin isoforms in Saccharomyces cerevisiae

1. Anubhav Dhar
2. VT Bagyashree
3. Sudipta Biswas
4. Jayanti Kumari
5. Amruta Sridhara
6. B Jeevan Subodh
7. Shashank Shekhar
8. Saravanan Palani

Reviewed by Review Commons

Reprograming gene expression in ‘hibernating’ C. elegans involves the IRE-1/XBP-1 pathway

1. Melanie Lianne Engelfriet
2. Yanwu Guo
3. Andreas Arnold
4. Eivind Valen
5. Rafal Ciosk

• Curated by eLife

  eLife Assessment

  This useful study reveals that as C. elegans, a poikilothermic ("cold-blooded") animal, adapt to cold (4ºC), they display a drastic reduction in translation (assessed by polysome profiling and SUNSET). The remaining translation (by ribo-seq) correlates with mRNA levels (by RNA-seq), and the changes in gene expression at least partially require IRE-1, an established endoplasmic reticulum stress sensor. The reviewers consider the data assessing global translation and RNA expression upon cold exposure and the data demonstrating the requirement of ire-1 to be solid, but the conclusion that "transcription" is the major regulatory step and "lipid changes" can be a signal for IRE-1 activation in cold adapted worms needs substantially more evidence. Overall, this study demonstrated a good correlation between translation and RNA levels and yielded an inventory of gene changes as C. elegans adapt to cold, and will be of general interest to researchers interested in stress response and cold adaptation.

Reviewed by eLife

Nucleoporin Nup153 docks the splicing machinery to the nuclear pore for efficient mRNA processing

1. IJ de Castro
2. L Schuster
3. C Patiño-Gomez
4. D Glavas
5. A Udupa
6. M Ríos Vázquez
7. T Symens
8. G Tulcan
9. J Heinze
10. Heras J de las
11. Robert Reinhardt
12. Jorge Trojanowski
13. H Harz
14. G Stumberger
15. H Leonhardt
16. E Schirmer
17. S Saka
18. V Laketa
19. M Lusic

Reviewed by Review Commons

Optogenetic and chemical genetic tools for rapid repositioning of vimentin intermediate filaments

1. Milena Pasolli
2. Joyce C. M. Meiring
3. James P. Conboy
4. Gijsje H. Koenderink
5. Anna Akhmanova

Reviewed by Review Commons

IFT20 regulates lymphatic endothelial cell-cell junctions via endocytic trafficking of VE-cadherin

1. Delayna Paulson
2. Ahana Majumder
3. Zachary Lehmann
4. Luke Knutson
5. Jacob Paulson
6. Shannon Lasey
7. Darci M. Fink

Reviewed by Review Commons

Treacle’s ability to form liquid-like phase condensates is essential for nucleolar fibrillar center assembly, efficient rRNA transcription and processing, and rRNA gene repair

1. Artem K Velichko
2. Nadezhda V Petrova
3. Dmitry A Deriglazov
4. Anastasia P Kovina
5. Artem V Luzhin
6. Eugene P Kazakov
7. Igor I Kireev
8. Sergey Razin
9. Omar L Kantidze

• Curated by eLife

  eLife Assessment

  This important study reveals that the nucleolar protein Treacle undergoes liquid-liquid phase separation in vitro and in vivo. It provides convincing evidence that the ability of Treacle to form phase-separated condensates is necessary for the proper formation of the fibrillar center of the nucleolus, rRNA transcription, and rDNA repair. These findings will be of interest to the communities studying biomolecular condensates, nucleolar organization, and ribosome biogenesis.

Reviewed by eLife

Teach your microscope how to print: Low-cost and rapid-iteration microfabrication for biology

1. Lucien Hinderling
2. Remo Hadorn
3. Moritz Kwasny
4. Joël Frei
5. Benjamin Grädel
6. Sacha Psalmon
7. Yannick Blum
8. Rémi Berthoz
9. Alex Landolt
10. Benjamin D. Towbin
11. Daniel Riveline
12. Olivier Pertz

Reviewed by Arcadia Science