Cell Biology

Microscopy Nodes: versatile 3D microscopy visualization with Blender

1. Oane Gros
2. Chandni Bhickta
3. Granita Lokaj
4. Yannick Schwab
5. Simone Köhler
6. Niccolò Banterle

Reviewed by Review Commons

Enalapril mitigates senescence and aging-related phenotypes in human cells and mice via pSmad1/5/9-driven antioxidative genes

1. Wencong Lyu
2. Haochen Wang
3. Zhehao Du
4. Ran Wei
5. Jianuo He
6. Fanju Meng
7. Jinlong Bi
8. Lijun Zhang
9. Chao Zhang
10. Yiting Guan
11. Wei Tao

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into the anti-senescence effects of enalapril, identifying pSmad1/5/9 signaling and associated antioxidant pathways as key mediators of its physiological benefits in aged mice. The authors present solid experimental evidence across both in vitro and in vivo systems, demonstrating improved organ function and reduced senescence markers following treatment. Overall, the work supports the repurposing potential of enalapril in aging research and expands understanding of its molecular targets.

Reviewed by eLife

Testosterone-induced metabolic changes in seminal vesicle epithelium modify seminal plasma components with potential to improve sperm motility

1. Takahiro Yamanaka
2. Zimo Xiao
3. Natsumi Tsujita
4. Mahmoud Awad
5. Takashi Umehara
6. Masayuki Shimada

• Curated by eLife

  eLife Assessment

  This important work elucidates the biological processes and detailed mechanisms by which testosterone influences seminal plasma metabolites in mice. The evidence supporting the upregulation of metabolic enzymes and the role of ACLY is solid, highlighting the potential contributions of fatty acids to sperm motility.

Reviewed by eLife

Organization of the Yeast Seipin Complex Reveals Differential Recruitment of Regulatory Proteins

1. Yoel A. Klug
2. Pedro Carvalho

Reviewed by Review Commons

The insulin / IGF axis is critically important controlling gene transcription in the podocyte

1. Jennifer A Hurcombe
2. Lusyan Dayalan
3. Fern Barrington
4. Frédéric Burdet
5. Lan Ni
6. Joseph T Coward
7. Paul T Brinkkoetter
8. Martin Holzenberger
9. Aaron Jeffries
10. Sebastian Oltean
11. Gavin I Welsh
12. Richard JM Coward

• Curated by eLife

  eLife Assessment

  This study investigated the role of insulin receptor (IR) and insulin-like growth factor 1 receptor (IGF1R) in the renal glomerular podocytes by characterizing the mice with dual deletion of both receptors in vivo as well as the cultured murine podocytes with induced deletion of both receptors in vitro. The solid data presented in this paper demonstrated the critical requirement of both IR and IGF1R signaling in normal podocyte physiology in mice, albeit a more detailed characterization of the mouse model is desired. Interestingly, long-range sequencing revealed significant retention of introns in mRNAs, due to an altered spliceosome level resulted from the loss of IR and IGF1 signaling in cultured podocytes. This new finding suggests an essential role of IR and IGF1R signaling in regulating RNA metabolism in podocyte, which provides useful information for the understanding of physiology and metabolism of podocytes. However, the underlying molecular mechanism for such a regulation is still unclear and awaits further studies.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife

A biochemical mechanism for Stu2/XMAP215-family microtubule polymerases

1. Binnu Gangadharan
2. Daniel L Kober
3. Luke M Rice

• Curated by eLife

  eLife Assessment

  In their important manuscript, Gangadharan, Kober and Rice focus on how Stu2/XMAP215-family microtubule polymerases use their TOG domains to catalytically promote microtubule growth, testing whether their mechanism follows an enzyme-like kinetic model similar to that of actin polymerases. The authors integrate measurements including microtubule polymerization rates and TOG-tubulin binding kinetics to convincingly show that Stu2 follows an enzyme-like model where tight tubulin binding enables efficient polymerization, revealing a shared mechanism with actin polymerases despite their evolutionary divergence. This work will be of general interest to the cell biology and biophysics communities.

Reviewed by eLife

Fertilization triggers cytosolic functions and P-body recruitment of the RNA-binding protein Mei2 to drive fission yeast zygotic development

1. Ayokunle Araoyinbo
2. Clàudia Salat-Canela
3. Aleksandar Vještica

Reviewed by Review Commons

Optogenetic control of mechanotransduction based on light-induced homodimerization of talin

1. Ryosuke Nishimura
2. Samuel F. H. Barnett
3. Kashish Jain
4. Zengxin Huang
5. Benjamin T. Goult
6. Pakorn Kanchanawong

Reviewed by preLights

Transcriptional dynamics uncover the role of BNIP3 in mitophagy during muscle remodeling in Drosophila

1. Hiroki Taoka
2. Tadayoshi Murakawa
3. Kohei Kawaguchi
4. Michiko Koizumi
5. Tatsuya Kaminishi
6. Yuriko Sakamaki
7. Kaori Tanaka
8. Akihito Harada
9. Keiichi Inoue
10. Tomotake Kanki
11. Yasuyuki Ohkawa
12. Naonobu Fujita

• Curated by eLife

  eLife Assessment

  This paper presents the important finding that BNIP3/NIX, a mitophagy receptor, and its binding to ATG18 are required for mitophagy during muscle cell reorganization in Drosophila. Although the involvement of the BNIP3-ATG18/WIPI axis in mitophagy induction has been reported in mammalian cell culture systems, this study provides the first compelling evidence for this pathway in vivo in animals. The physiological significance of this BNIP3-dependent mitophagy will require further investigation.

Reviewed by eLife

ATG2A engages RAB1A and ARFGAP1 positive membranes during autophagosome biogenesis

1. Devin M Fuller
2. Yumei Wu
3. Florian Schueder
4. Burha Rasool
5. Shanta Nag
6. Justin L Korfhage
7. Rolando Garcia-Milian
8. Katerina D Melnyk
9. Joerg Bewersdorf
10. Pietro De Camilli
11. Thomas J Melia

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into the crosstalk between ATG2A with components of the early secretory pathway, namely RAB1A and ARFGAP1. The evidence supporting the claims is convincing. However, the manuscript would benefit from a more in-depth exploration of the details of the role of RAB1A in autophagy and the functional implications of its interaction with ATG2A. In addition, the molecular details of the role of ARFGAP1 in this complex need further clarification

Reviewed by eLife