Cell Biology

Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)

1. Maria Llamazares Prada
2. Uwe Schwartz
3. Darius F. Pease
4. Stephanie T. Pohl
5. Deborah Ackesson
6. Renjiao Li
7. Annika Behrendt
8. Raluca Tamas
9. Mandy Richter
10. Thomas Muley
11. Joschka Hey
12. Elisa Espinet
13. Claus P. Heußel
14. Arne Warth
15. Mark Schneider
16. Hauke Winter
17. Felix Herth
18. Charles D Imbusch
19. Benedikt Brors
20. Vladimir Benes
21. David Wyatt
22. Tomasz P. Jurkowski
23. Heiko F. Stahl
24. Christoph Plass
25. Renata Z. Jurkowska

Reviewed by Review Commons

CCDC32 stabilizes clathrin-coated pits and drives their invagination

1. Ziyan Yang
2. Changsong Yang
3. Zheng Huang
4. Peiliu Xu
5. Yueping Li
6. Lu Han
7. Linyuan Peng
8. Xiangying Wei
9. John E Pak
10. Tatyana Svitkina
11. Sandra L Schmid
12. Zhiming Chen

• Curated by eLife

  eLife Assessment

  The manuscript presents a valuable finding that CCDC32, beyond its reported role in AP2 assembly, follows AP2 to the plasma membrane and regulates clathrin-coated pit assembly and dynamics. The authors further identify an alpha-helical region within CCDC32 that is essential for its interaction with AP2 and its cellular function. While live-cell and ultrastructural imaging data are solid, future biochemical studies will be needed to confirm the proposed CCDC32-AP2 interaction.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Wnt induces FZD5/8 endocytosis and degradation and the involvement of RSPO-ZNRF3/RNF43 and DVL

1. Dong Luo
2. Jing Zheng
3. Shuning Lv
4. Ren Sheng
5. Maorong Chen
6. Xi He
7. Xinjun Zhang

• Curated by eLife

  eLife Assessment

  This study presents important findings demonstrating that the internalization and degradation of FZD5 and FZD8, two of the ten Frizzled proteins, are WNT dependent and do not involve DVL. The evidence supporting the claims of the authors is convincing. This research will be of interest to biologists specializing in Wnt signaling, cancer, and regenerative medicine.

Reviewed by eLife

RNA triggers chronic stress during neuronal aging

1. Kevin Rhine
2. Elle Epstein
3. Natasha M. Carlson
4. Xuezhen Ge
5. Orel Mizrahi
6. Anika Kamat
7. Anita Hermann
8. William R. Brothers
9. John Ravits
10. Eric J. Bennett
11. Gülçin Pekkurnaz
12. Gene W. Yeo

Reviewed by PREreview

Microscopy Nodes: versatile 3D microscopy visualization with Blender

1. Oane Gros
2. Chandni Bhickta
3. Granita Lokaj
4. Yannick Schwab
5. Simone Köhler
6. Niccolò Banterle

Reviewed by Review Commons

Enalapril mitigates senescence and aging-related phenotypes in human cells and mice via pSmad1/5/9-driven antioxidative genes

1. Wencong Lyu
2. Haochen Wang
3. Zhehao Du
4. Ran Wei
5. Jianuo He
6. Fanju Meng
7. Jinlong Bi
8. Lijun Zhang
9. Chao Zhang
10. Yiting Guan
11. Wei Tao

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into the anti-senescence effects of enalapril, identifying pSmad1/5/9 signaling and associated antioxidant pathways as key mediators of its physiological benefits in aged mice. The authors present solid experimental evidence across both in vitro and in vivo systems, demonstrating improved organ function and reduced senescence markers following treatment. Overall, the work supports the repurposing potential of enalapril in aging research and expands understanding of its molecular targets.

Reviewed by eLife

Testosterone-induced metabolic changes in seminal vesicle epithelium modify seminal plasma components with potential to improve sperm motility

1. Takahiro Yamanaka
2. Zimo Xiao
3. Natsumi Tsujita
4. Mahmoud Awad
5. Takashi Umehara
6. Masayuki Shimada

• Curated by eLife

  eLife Assessment

  This important work elucidates the biological processes and detailed mechanisms by which testosterone influences seminal plasma metabolites in mice. The evidence supporting the upregulation of metabolic enzymes and the role of ACLY is solid, highlighting the potential contributions of fatty acids to sperm motility.

Reviewed by eLife

Organization of the Yeast Seipin Complex Reveals Differential Recruitment of Regulatory Proteins

1. Yoel A. Klug
2. Pedro Carvalho

Reviewed by Review Commons

The insulin / IGF axis is critically important controlling gene transcription in the podocyte

1. Jennifer A Hurcombe
2. Lusyan Dayalan
3. Fern Barrington
4. Frédéric Burdet
5. Lan Ni
6. Joseph T Coward
7. Paul T Brinkkoetter
8. Martin Holzenberger
9. Aaron Jeffries
10. Sebastian Oltean
11. Gavin I Welsh
12. Richard JM Coward

• Curated by eLife

  eLife Assessment

  This study investigated the role of insulin receptor (IR) and insulin-like growth factor 1 receptor (IGF1R) in the renal glomerular podocytes by characterizing the mice with dual deletion of both receptors in vivo as well as the cultured murine podocytes with induced deletion of both receptors in vitro. The solid data presented in this paper demonstrated the critical requirement of both IR and IGF1R signaling in normal podocyte physiology in mice, albeit a more detailed characterization of the mouse model is desired. Interestingly, long-range sequencing revealed significant retention of introns in mRNAs, due to an altered spliceosome level resulted from the loss of IR and IGF1 signaling in cultured podocytes. This new finding suggests an essential role of IR and IGF1R signaling in regulating RNA metabolism in podocyte, which provides useful information for the understanding of physiology and metabolism of podocytes. However, the underlying molecular mechanism for such a regulation is still unclear and awaits further studies.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife

A biochemical mechanism for Stu2/XMAP215-family microtubule polymerases

1. Binnu Gangadharan
2. Daniel L Kober
3. Luke M Rice

• Curated by eLife

  eLife Assessment

  In their important manuscript, Gangadharan, Kober and Rice focus on how Stu2/XMAP215-family microtubule polymerases use their TOG domains to catalytically promote microtubule growth, testing whether their mechanism follows an enzyme-like kinetic model similar to that of actin polymerases. The authors integrate measurements including microtubule polymerization rates and TOG-tubulin binding kinetics to convincingly show that Stu2 follows an enzyme-like model where tight tubulin binding enables efficient polymerization, revealing a shared mechanism with actin polymerases despite their evolutionary divergence. This work will be of general interest to the cell biology and biophysics communities.

Reviewed by eLife