Cell Biology

Neutrophils actively swell to potentiate rapid migration

1. Tamas L Nagy
2. Evelyn Strickland
3. Orion D Weiner

• Curated by eLife

  eLife assessment

  This fundamental study significantly advances our understanding of the role of water influx and swelling on neutrophil migration in response to chemoattractant. The evidence supporting the conclusions, based on a genome-wide CRISPR screen and high quality cellular observations, is compelling. This paper will be of interest to cell biologists and biophysicists working on cell migration.

Reviewed by eLife

Dietary bacteria control C. elegans fat content through pathways converging at phosphatidylcholine

1. Hsiao-Fen Han
2. Shao-Fu Nien
3. Hang-Shiang Jiang
4. Jui-Ching Wu
5. Chia-Yi Chiang
6. Man-Tzu Li
7. Leng-Jie Huang
8. Sufeng Chiang
9. Lien-Chieh Lin
10. Yi-Ting Chuang
11. Yu-Ho Lin
12. Chao-Wen Wang
13. Yi-Chun Wu

• Curated by eLife

  eLife assessment

  This a useful study that reports a genetic regulatory network that accounts for altered lipid metabolism in response to two different bacterial diets of C. elegans. The proposed mechanism, linking vitamin B12, S-adenosyl methionine (SAM), phosphatidylcholine (PC), and neutral lipid levels, is solid but has been previously demonstrated by other studies using similar assays. The evidence to support a new layer of regulation, via the production of phospho-choline by ASM-3/acid sphingomyelinase, requires further substantiation.

Reviewed by eLife

Coordination between cytoskeletal organization, cell contraction and extracellular matrix development, is depended on LOX for aneurysm prevention

1. Rohtem Aviram
2. Shelly Zaffryar-Eilot
3. Anna Kaganovsky
4. Anas Odeh
5. Shay Melamed
6. Ruslana Militsin
7. Cameron B. Pinnock
8. Ariel Shemesh
9. Raz Palty
10. Santhi K. Ganesh
11. Peleg Hasson

Reviewed by Review Commons

Identification of a third myosin-5a-melanophilin interaction that mediates the association of myosin-5a with melanosomes

1. Jiabin Pan
2. Rui Zhou
3. Lin-Lin Yao
4. Jie Zhang
5. Ning Zhang
6. Qing-Juan Cao
7. Shaopeng Sun
8. Xiang-dong Li

• Curated by eLife

  eLife assessment

  This study represents a useful description of a third interaction site between melanophilin and myosin-5a which has a role in regulating the distribution of pigment granules in melanocytes. While much of the data forms a solid case for this interaction, the inclusion of controls for the cellular studies and measurement of interaction affinities would have been helpful.

Reviewed by eLife

Engineered Migrasomes: A Robust, Thermally Stable Vaccination Platform

1. Dongju Wang
2. Haifang Wang
3. Wei Wan
4. Zihui Zhu
5. Takami Sho
6. Yi Zheng
7. Xing Zhang
8. Longyu Dou
9. Qiang Ding
10. Li Yu
11. Zhihua Liu

• Curated by eLife

  eLife assessment

  The study, from the group that pioneered migrasome, describes a novel vaccine platform derived from this newly discovered organelle. Using these cleverly engineered migrasomes – that behave like natural migrasomes – as a novel vaccine platform has the potential to overcome obstacles such as cold chain issues for vaccines like messenger RNA. Although the findings are important with practical implications for the vaccine technology, and the evidence, based on appropriate and validated methodology is convincing and is in line with current state-of-the-art, there are some critical issues that need to be addressed. These include a head-to-head comparison with proven vaccine platforms, for example, a SARS-CoV-2 mRNA vaccine or an adjuvanted recombinant spike protein.

Reviewed by eLife

Re-assessment of the subcellular localization of Bazooka/Par-3 in Drosophila : No evidence for localization to the nucleus and the neuromuscular junction

1. Soya Kim
2. Jaffer Shahab
3. Andreas Wodarz

Reviewed by Review Commons

The non-mitotic role of HMMR in regulating the localization of TPX2 and the dynamics of microtubules in neurons

1. Yi-Ju Chen
2. Shun-Cheng Tseng
3. Peng-Tzu Chen
4. Eric Hwang

• Curated by eLife

  eLife assessment

  In their valuable study, Chen et al. investigate the neuronal role of HMMR, a microtubule-associated protein typically associated with cell division. Their findings indicate that HMMR is necessary for proper neuronal morphology and the generation of polymerizing microtubules within neurites, potentially by promoting the function of TPX2. This solid body of work is the first step in deciphering the influence of a mitotic microtubule-associated protein in organizing microtubules in neurons and will be of interest to the neurobiology and cytoskeleton fields.

Reviewed by eLife

A high-throughput platform for single-molecule tracking identifies drug interaction and cellular mechanisms

1. David T. McSwiggen
2. Helen Liu
3. Ruensern Tan
4. Sebastia Agramunt Puig
5. Lakshmi B. Akella
6. Russell Berman
7. Mason Bretan
8. Hanzhe Chen
9. Xavier Darzacq
10. Kelsey Ford
11. Ruth Godbey
12. Eric Gonzalez
13. Adi Hanuka
14. Alec Heckert
15. Jaclyn J. Ho
16. Stephanie L. Johnson
17. Reed Kelso
18. Aaron Klammer
19. Ruchira Krishnamurthy
20. Jifu Li
21. Kevin Lin
22. Brian Margolin
23. Patrick McNamara
24. Laurence Meyer
25. Sarah E. Pierce
26. Akshay Sule
27. Connor Stashko
28. Yangzhong Tang
29. Daniel J. Anderson
30. Hilary P. Beck

• Curated by eLife

  eLife assessment

  This work presents an important technological advance, in the form of a high throughput platform for Single Particle Tracking allowing us to measure millions of cells and thousands of compounds per day. Analysis of the diffusional behaviour of fluorescently-tagged targets permits the identification of, and differentiation between, small molecules that bind directly or affect the target indirectly. The methodology and metrics employed are compelling, leading to the identification of multiple compounds that effectively change the diffusive state of the estrogen receptor, the POC target of the study.

Reviewed by eLife

Clearance of protein aggregates during cell division

1. Shoukang Du
2. Yuhan Wang
3. Bowen Chen
4. Shuangshuang Xie
5. Kuan Yoow Chan
6. David C. Hay
7. Ting Gang Chew

• Curated by eLife

  eLife assessment

  How misfolded proteins are segregated and cleared is a significant question in mechanistic cell biology, since clearance of these aggregates can protect against pathologies that may otherwise arise. The authors discover a cell cycle stage-dependent clearing mechanism that involves the ER chaperone BiP, the proteosome, and CDK inactivation, but is curiously independent of the APC. These are valuable and interesting new observations, but the evidence supporting these claims is incomplete, and needs to be strengthened and further validated.

Reviewed by eLife

Microtubule-dependent orchestration of centriole amplification in brain multiciliated cells

1. Amélie-Rose Boudjema
2. Rémi Balagué
3. Cayla E Jewett
4. Gina M LoMastro
5. Olivier Mercey
6. Adel Al Jord
7. Marion Faucourt
8. Alexandre Schaeffer
9. Camille Noûs
10. Nathalie Delgehyr
11. Andrew J Holland
12. Nathalie Spassky
13. Alice Meunier

• Curated by eLife

  eLife assessment

  In this important study, Boudjema et al. use cell culture models and advanced microscopic imaging to provide detailed analyses of the cellular events underlying centriole amplification, apical migration, and assembly of hundreds of motile cilia in multi-ciliated cells. This largely descriptive work provides a better understanding of this process that is of interest to cell biologists studying centrioles and cilia. Most of the claims are supported by the data, but the study would benefit from additional analyses regarding the roles of microtubules, which are currently incomplete, and from text editing to improve accessibility and readability, especially for a wider audience.

Reviewed by eLife