Cell Biology

Engineered migrasomes provide a robust and thermally stable vaccination platform

1. Dongju Wang
2. Haifang Wang
3. Wei Wan
4. Zihui Zhu
5. Takami Sho
6. Yi Zheng
7. Xing Zhang
8. Longyu Dou
9. Qiang Ding
10. Li Yu
11. Zhihua Liu

• Curated by eLife

  eLife Assessment

  This study, from the group that pioneered migrasome, describes a novel vaccine platform of engineered migrasomes that behave like natural migrasomes. Importantly, this platform has the potential to overcome obstacles associated with cold chain issues for vaccines such as mRNA. In the revised version, the authors have addressed previous concerns and the results from additional experiments provide compelling evidence that features methods, data, and analyses more rigorous than the current state-of-the-art. Although the findings are important with practical implications for the vaccine technology, results from additional experiments would make this an outstanding study.

Reviewed by eLife

Hexokinase regulates Mondo-mediated longevity via the PPP and organellar dynamics

1. Raymond Laboy
2. Marjana Ndoci
3. Shamsh Tabrez Syed
4. Maximilian Vonolfen
5. Eugen Ballhysa
6. Tim Droth
7. Klara Schilling
8. Anna Loehrke
9. Ilian Atanassov
10. Adam Antebi

• Curated by eLife

  eLife Assessment

  This important study utilizes the nematode C. elegans and mammalian cell culture to investigate the role of MML-1/Mondo in conserved regulation of metabolism and aging. The evidence supporting the conclusions is convincing and covers a range of areas including localization, upstream pathways, and conservation. The paper will be of interest to a broad range of biologists studying aging, metabolism, and transcriptional regulation.

Reviewed by eLife

Forward genetics in C. elegans reveals genetic adaptations to polyunsaturated fatty acid deficiency

1. Delaney Kaper
2. Uroš Radović
3. Per-Olof Bergh
4. August Qvist
5. Marcus Henricsson
6. Jan Borén
7. Marc Pilon

• Curated by eLife

  eLife Assessment

  This fundamental study investigates the role of polyunsaturated fatty acids (PUFAs) in physiology and membrane biology, using a unique model to perform a thorough genetic screen that demonstrates that PUFA synthesis defects cannot be compensated for by mutations in other pathways. These findings are supported by compelling evidence from a high quality genetic screen, functional validation of their hits, and lipid analyses. This study will appeal to researchers in membrane biology, lipid metabolism, and C. elegans genetics.

Reviewed by eLife

Rho-ROCK liberates sequestered claudin for rapid de novo tight junction formation

1. Yuma Cho
2. Akari Taniguchi
3. Akiharu Kubo
4. Junichi Ikenouchi

• Curated by eLife

  eLife Assessment

  This paper identifies a crucial step in the regulation of tight junction formation by identifying Rho-ROCK activity-dependent activation of the serine protease Matriptase, making Claudins available for tight junction formation. The reviewers were satisfied with the revisions and found the work important and the approach convincing.

Reviewed by eLife

Targeting the WSB2–NOXA axis in cancer cells for enhanced sensitivity to BCL-2 family protein inhibitors

1. Dongyue Jiao
2. Kun Chang
3. Jiamin Jin
4. Yingji Chen
5. Mo Ren
6. Yucong Zhang
7. Kun Gao
8. Yaoting Xu
9. Lixin Wang
10. Chenji Wang

• Curated by eLife

  eLife Assessment

  This study reports a fundamental observation concerning cell death regulation by the anti-apoptotic BCL2 family NOXA. The authors convincingly demonstrate that NOXA is destabilized through the interaction with WSB2, a substrate receptor in CRL5 ubiquitin ligase complex, sensitizing the cells to treatments. These are key findings for cell biologists and cancer researchers as they identified a new target impacting drug responsiveness in cancer therapies.

Reviewed by eLife

Human formin FHOD3-mediated actin elongation is required for sarcomere integrity in cardiomyocytes

1. Dylan A Valencia
2. Angela N Koeberlein
3. Haruko Nakano
4. Akos Rudas
5. Aanand A Patel
6. Airi Harui
7. Cassandra Spencer
8. Atsushi Nakano
9. Margot E Quinlan

• Curated by eLife

  eLife Assessment

  Valencia et al. combine elegant in vitro biochemical experiments with functional assays in cardiomyocytes to determine which properties of the FHOD3 formin are essential for sarcomere assembly. Using separation-of-function mutants, they show that FHOD3's elongation activity, rather than its nucleation, capping, or bundling activities, is key to its sarcomeric function. This is an important finding and the data presented in the manuscript are convincing; however, the presence of FHOD3 at filament barbed ends in the TIRF elongation assays should probably be verified directly in a future study.

Reviewed by eLife

Mitochondrial fission during mitophagy requires both inner and outer mitofissins

1. Kentaro Furukawa
2. Tatsuro Maruyama
3. Shun-ichi Yamashita
4. Keiichi Inoue
5. Tomoyuki Fukuda
6. Nobuo N. Noda
7. Tomotake Kanki

Reviewed by Review Commons

Cardiac neurons expressing a glucagon-like receptor mediate cardiac arrhythmia induced by high-fat diet in Drosophila

1. Yunpo Zhao
2. Jianli Duan
3. Joyce van de Leemput
4. Zhe Han

• Curated by eLife

  eLife Assessment

  This study reports useful information on the mechanisms by which a high-fat diet induces arrhythmias in the model organism Drosophila. Specifically, the authors propose that adipokinetic hormone (Akh) secretion is increased with this diet, and through binding of Akh to its receptor on cardiac neurons, arrhythmia is induced. The authors have revised their manuscript but the evidence remains incomplete. Nonetheless, the data presented will be helpful to those who wish to extend the research to a more complex model system, such as the mouse.

Reviewed by eLife

Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi

1. Hieng Chiong Tie
2. Haiyun Wang
3. Divyanshu Mahajan
4. Hilbert Yuen In Lam
5. Xiuping Sun
6. Bing Chen
7. Yuguang Mu
8. Lei Lu

• Curated by eLife

  eLife Assessment

  This important study offers convincing evidence that intra-Golgi transport slows from cis to trans and varies between cargos even within the same cisternae, supporting a more stable compartment model. Using nocodazole-induced ministacks, the authors show cargo-specific transport kinetics with distinct velocities and residence times. These findings refine the cisternal progression model and prompt further investigation into alternative mechanisms, such as rapid partitioning or rim progression. This study will be of interest to cell biologists studying membrane trafficking, Golgi organization, and protein secretion, as well as researchers investigating the mechanisms of organelle dynamics and the molecular basis of intracellular transport.

Reviewed by eLife

Optogenetic actin network assembly on lipid bilayer uncovers the network density-dependent functions of actin-binding proteins

1. Kei Yamamoto
2. Makito Miyazaki

Reviewed by Arcadia Science