Cell Biology

ATG2A engages RAB1A and ARFGAP1 positive membranes during autophagosome biogenesis

1. Devin M Fuller
2. Yumei Wu
3. Florian Schueder
4. Burha Rasool
5. Shanta Nag
6. Justin L Korfhage
7. Rolando Garcia-Milian
8. Katerina D Melnyk
9. Joerg Bewersdorf
10. Pietro De Camilli
11. Thomas J Melia

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into the crosstalk between ATG2A with components of the early secretory pathway, namely RAB1A and ARFGAP1. The evidence supporting the claims is convincing. However, the manuscript would benefit from a more in-depth exploration of the details of the role of RAB1A in autophagy and the functional implications of its interaction with ATG2A. In addition, the molecular details of the role of ARFGAP1 in this complex need further clarification

Reviewed by eLife

Live-cell imaging reveals nutrient-dependent dynamics of ER-mitochondria contact formation via PDZD8

1. Saeko Aoyama-Ishiwatari
2. Koki Nakamura
3. Takahiro Nagao
4. Yusuke Hirabayashi

Reviewed by Review Commons

G1 and G2 ApolipoproteinL1 modulate macrophage inflammation and lipid accumulation through the polyamine pathway

1. Esther Liu
2. Matthew Wright
3. Andrew O Kearney
4. Tiffany Caza
5. Johnson Y Yang
6. Valerie Garcia
7. Amal O Dadi
8. Shuta Ishibe
9. Navdeep S Chandel
10. Hanrui Zhang
11. Edward B Thorp
12. Jennie Lin

• Curated by eLife

  eLife Assessment

  The authors study how apolipoprotein L1 variants impact inflammation and lipid accumulation in macrophages. The findings will be useful for researchers investigating macrophage metabolism and inflammation. The discovery that the polyamine spermidine in part mediates such effects is interesting, but the supporting evidence for a physiologically relevant role is currently incomplete due to the lack of relevant in vivo studies.

Reviewed by eLife

Impact of maximal overexpression of a non-toxic protein on yeast cell physiology

1. Yuri Fujita
2. Shotaro Namba
3. Yoshiaki Kamada
4. Hisao Moriya

• Curated by eLife

  eLife assessment

  This convincing study advances our understanding of the physiological consequences of the strong overexpression of non-toxic proteins in baker's yeast. The findings suggest that a massive protein burden results in nitrogen starvation and a shift in metabolism likely regulated via the TORC1 pathway, as well as defects in ribosome biogenesis in the nucleolus. The study presents findings and tools that are important for the cell biology and protein homeostasis fields.

Reviewed by eLife

C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin

1. Jessica Mella
2. Regan F Volk
3. Balyn W Zaro
4. Abigail Buchwalter

• Curated by eLife

  eLife Assessment

  This study presents a valuable finding on the delivery of a nuclear envelop protein to lysosomes and the impact of C-terminal tagging on its traffic. The authors provide solid evidence for the potential artifacts introduced by large terminal tags, particularly in the context of membrane protein localization and stability.

Reviewed by eLife

Novel Fatigue Profiling Approach Highlights Temporal Dynamics of Human Sperm Motility

1. Athanasia Sergounioti
2. Efstathios Alonaris
3. Dimitrios Rigas

Reviewed by PREreview

Tmbim5 and Slc8b1 cooperate in tissue-specific mitochondrial calcium regulation in zebrafish

1. Iga Wasilewska
2. Łukasz Majewski
3. Dobrochna Adamek-Urbańska
4. Sofiia Baranykova
5. Matylda Macias
6. Aleksandra Szybińska
7. Axel Methner

Reviewed by Review Commons

Key roles of the zona pellucida and perivitelline space in promoting gamete fusion and fast block to polyspermy inferred from the choreography of spermatozoa in mice oocytes

1. Yaëlle Dubois
2. Sophie Favier
3. Nathan Martin-Fornier
4. Mohyeddine Omrane
5. David Stroebel
6. Eric Perez
7. Sandrine Barbaux
8. Ahmed Ziyyat
9. Nicolas Rodriguez
10. Christine Gourier

Reviewed by Review Commons

CellBinDB: a large-scale multimodal annotated dataset for cell segmentation with benchmarking of universal models

1. Can Shi
2. Jinghong Fan
3. Zhonghan Deng
4. Huanlin Liu
5. Qiang Kang
6. Yumei Li
7. Jing Guo
8. Jingwen Wang
9. Jinjiang Gong
10. Sha Liao
11. Ao Chen
12. Ying Zhang
13. Mei Li

Reviewed by GigaScience

TORC2-regulated sterol redistribution mediates recovery from membrane perturbation by small amphipathic molecules.

1. Maria G. Tettamanti
2. Paulina Nowak
3. Beata Kusmider
4. Jennifer M Kefauver
5. Vincent Mercier
6. Aurelien Roux
7. Robbie Loewith

Reviewed by Review Commons