Cell Biology

Bridging the Gap in Cancer Cell Behavior Against Matrix Stiffening: Insights from a Trizonal Model

1. Mohammad E Torki
2. Fan Liu
3. Rongguang Xu
4. Yunfeng Chen
5. Jeffery Fredberg
6. Zi Chen

• Curated by eLife

  eLife Assessment

  This study presents a valuable nonlinear mathematical model that addresses how cell shape transitions in response to ECM stiffness. The evidence supporting the claims of the authors is solid, although additional work is required to improve the manuscript. For instance, the authors should improve the overall readability of the text and amend the experimental validation section. The work will be of interest to scientists working on a spectrum of fields including cell mechanics, cell behaviors, and cancer research.

Reviewed by eLife

Fis1 is required for the development of the dendritic mitochondrial network in pyramidal cortical neurons

1. Klaudia Strucinska
2. Parker Kneis
3. Travis Pennington
4. Katarzyna Cizio
5. Patrycja Szybowska
6. Abigail Morgan
7. Joshua Weertman
8. Tommy L Lewis

Reviewed by preLights

Identification of a sub-population of synovial mesenchymal stem cells with enhanced treatment efficacy in a rat model of Osteoarthritis

1. Nedaa Al-Jezani
2. Asmaa Affan
3. Catherine Leonard
4. Nabangshu Das
5. Luiz Gustavo Almeida
6. Daniel Young
7. Anand O Masson
8. Antoine Dufour
9. Paul Salo
10. Pam Railton
11. James N Powell
12. Roman J Krawetz

• Curated by eLife

  eLife Assessment

  This important study focused on characterizing clonally derived MSC populations from the synovium of normal and osteoarthritis (OA) patients, demonstrating their potential to regenerate cartilage in vivo. Although the strength of evidence is solid, further work is needed to fill the gaps in the CD47Hi cell characterization and the in vivo response assessment. The study will be of interest to scientists advancing MSC based regenerative medicine approaches.

Reviewed by eLife

SRS microscopy identifies inhibition of vitellogenesis as a mediator of lifespan extension by caloric restriction in C. elegans

1. Bowen Yang
2. Bryce Manifold
3. Wuji Han
4. Catherin DeSousa
5. Wanyi Zhu
6. Aaron Streets
7. Denis V. Titov

Reviewed by Arcadia Science

MuSpAn: A Toolbox for Multiscale Spatial Analysis

1. Joshua A. Bull
2. Joshua W. Moore
3. Eoghan J. Mulholland
4. Simon J. Leedham
5. Helen M. Byrne

Reviewed by Arcadia Science

Protein absorption in the zebrafish gut is regulated by interactions between lysosome rich enterocytes and the microbiome

1. Laura Childers
2. Jieun Park
3. Siyao Wang
4. Richard Liu
5. Robert Barry
6. Stephen A Watts
7. John F Rawls
8. Michel Bagnat

• Curated by eLife

  eLife Assessment

  In this study, the authors use the zebrafish to investigate how the microbiome affects a specialized gut cell called the lysosome rich enterocyte. They use a combination of functional assays for protein absorption, gnotobiotic manipulations and single-cell RNA-seq. The findings in the paper are considered important and the results are convincing.

Reviewed by eLife

RAB18 Orchestrates Signaling Protein Ciliary Homeostasis by Facilitating BBSome Diffusion through the Transition Zone for Ciliary Entry

1. Wei-Yue Sun
2. Jingchao Zhang
3. Yan Wei
4. Linda S. Zhao
5. Zhen-Chuan Fan

Reviewed by Arcadia Science

Endosomal chemokine receptor signalosomes regulate central mechanisms underlying cell migration

1. Hyunggu Hahn
2. Carole Daly
3. John Little
4. Nicole A Perry-Hauser
5. Emmanuel Flores-Espinoza
6. Asuka Inoue
7. Bianca Plouffe
8. Alex RB Thomsen

• Curated by eLife

  eLife Assessment

  This is an important study that provides CCR7-APEX2 proximity labelling mass spectrometry data that is expected to provide new insights into CCR7 signaling partners and pathways. The study is technically easy to follow and the data is convincing. It will be interesting in the future to have complementary studies in lymphocytes/dendritic cells that endogenously express CCR7. This is of value to the community, and there are likely multiple opportunities to use the APEX2 data set to extend these findings, strengthen some claims, and even explore a new pathway identified in the APEX2 data set.

Reviewed by eLife

The conserved ATPase PCH-2 controls the number and distribution of crossovers by antagonizing their formation in Caenorhabditis elegans

1. Bhumil Patel
2. Maryke Grobler
3. Alberto Herrera
4. Elias Logari
5. Valery Ortiz
6. Needhi Bhalla

• Curated by eLife

  eLife Assessment

  This is an important study examining the role of conserved PCH-2 protein at different stages of C. elegans meiosis. The authors use elegant molecular genetic approaches to provide convincing evidence to support their claims. The work will be of interest to scientists studying meiosis, DNA recombination, and chromosome segregation.

Reviewed by eLife

Optogenetic control of a GEF of RhoA uncovers a signaling switch from retraction to protrusion

1. Jean de Seze
2. Maud Bongaerts
3. Benoit Boulevard
4. Mathieu Coppey

• Curated by eLife

  eLife Assessment

  This important study combines compelling experiments with optogenetic actuation and convincing theory to understand how signalling proteins control the switch between cell protrusion and retraction, two essential processes in single cell migration. The authors examine the importance of the basal concentration and recruitment dynamics of a guanine exchange factor (GEF) on the activity of the downstream effectors RhoA and Cdc42, which control retraction and protrusion. The experimental and theoretical evidence provides a model of RhoA's involvement in both protrusion and retraction and shows that these complex processes are highly dependent on the concentration and activity dynamics of the components.

Reviewed by eLife