Cell Biology

SARS-CoV-2 envelope protein induces LC3 lipidation via the V-ATPase-ATG16L1 axis

1. Carmen Figueras-Novoa
2. Lewis Timimi
3. Elena Marcassa
4. Raquel Taveira-Marques
5. Lorin Adams
6. Ming Jiang
7. Mary Wu
8. Beatriz Montaner
9. Kevin Ng
10. Giuditta De Lorenzo
11. Wilhelm Furnon
12. Vanessa M. Cowton
13. Nicole Upfold
14. George Kassiotis
15. Ruth Harvey
16. Arvind H Patel
17. Michael Howell
18. Rachel Ulferts
19. Rupert Beale

Reviewed by Review Commons

Cell Adhesion-Dependent Biphasic Axon Outgrowth Elucidated by Femtosecond Laser Impulse

1. Sohei Yamada
2. Kentarou Baba
3. Naoyuki Inagaki
4. Yoichiroh Hosokawa

• Curated by eLife

  eLife Assessment

  Axon growth is essential to formation of neural connections. This manuscript presents a useful presentation of a new method for assessing the adhesion strength of axons with the use of a laser-induced shock wave. However, the strength of the evidence is incomplete as critical controls for calibration and time course are lacking.

Reviewed by eLife

A single factor for safer cellular rejuvenation

1. Lucas Paulo de Lima Camillo
2. Rihab Gam
3. Katsiaryna Maskalenka
4. Francis J. A. LeBlanc
5. Gustavo Antonio Urrutia
6. Gabriel M. Mejia
7. Henry E. Miller
8. Christopher P. Wardlaw
9. Adam Pickles
10. Laura Everton
11. Ringaile Zaksauskaite
12. Rejina B. Khan
13. Andreas Welsh
14. Samira Gambo
15. Stephany Gallardo
16. Zoryana Oliynyk
17. Sagar S. Varankar
18. Alexander E. Epstein
19. Adam Bendall
20. Jonathan Mowatt
21. Daniel Ives
22. Brendan M. Swain

Reviewed by PREreview

PHD1-dependent hydroxylation of RepoMan (CDCA2) on P604 modulates the control of mitotic progression

1. Jimena Druker
2. Hao Jiang
3. Dilem Shakir
4. Fraser Child
5. Vanesa Alvarez
6. Melpomeni Platani
7. Andrea Corno
8. Constance Alabert
9. Adrian T Saurin
10. Jason R Swedlow
11. Sonia Rocha
12. Angus I Lamond

• Curated by eLife

  eLife Assessment

  This compelling work describes how the cell cycle-regulating phosphatase subunit, RepoMan, is regulated by the oxygen-dependent, metabolite-sensing hydroxylase PHD1. The characterisation of how proline hydroxylation alters signalling at the molecular and cellular level provides important evidence to enhance our understanding of how 2-oxoglutarate-dependent dioxygenases influence the cell cycle and mitosis.

Reviewed by eLife

SARS-CoV-2 NSP13 interacts with TEAD to suppress Hippo-YAP signaling

1. Fansen Meng
2. Jong Hwan Kim
3. Chang-Ru Tsai
4. Jeffrey D Steimle
5. Jun Wang
6. Yufeng Shi
7. Rich G Li
8. Bing Xie
9. Vaibhav Deshmukh
10. Shijie Liu
11. Xiao Li
12. James F Martin

• Curated by eLife

  eLife Assessment

  This important study elucidates the molecular function of the SARS-CoV-2 helicase NSP13, which inhibits the transcriptional activity of the YAP/TEAD complex in vitro and in vivo. The evidence supporting the authors' claims is compelling, based on cell biological assays and multi-omic studies. This work contributes to the understanding of the new regulatory mechanism of YAP/TEAD after SARS-CoV-2 infection and will be of interest to researchers investigating COVID-19 infection and the Hippo-YAP signaling pathway.

Reviewed by eLife

Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)

1. Maria Llamazares Prada
2. Uwe Schwartz
3. Darius F. Pease
4. Stephanie T. Pohl
5. Deborah Ackesson
6. Renjiao Li
7. Annika Behrendt
8. Raluca Tamas
9. Mandy Richter
10. Thomas Muley
11. Joschka Hey
12. Elisa Espinet
13. Claus P. Heußel
14. Arne Warth
15. Mark Schneider
16. Hauke Winter
17. Felix Herth
18. Charles D Imbusch
19. Benedikt Brors
20. Vladimir Benes
21. David Wyatt
22. Tomasz P. Jurkowski
23. Heiko F. Stahl
24. Christoph Plass
25. Renata Z. Jurkowska

Reviewed by Review Commons

CCDC32 stabilizes clathrin-coated pits and drives their invagination

1. Ziyan Yang
2. Changsong Yang
3. Zheng Huang
4. Peiliu Xu
5. Yueping Li
6. Lu Han
7. Linyuan Peng
8. Xiangying Wei
9. John E Pak
10. Tatyana Svitkina
11. Sandra L Schmid
12. Zhiming Chen

• Curated by eLife

  eLife Assessment

  The manuscript presents a valuable finding that CCDC32, beyond its reported role in AP2 assembly, follows AP2 to the plasma membrane and regulates clathrin-coated pit assembly and dynamics. The authors further identify an alpha-helical region within CCDC32 that is essential for its interaction with AP2 and its cellular function. While live-cell and ultrastructural imaging data are solid, future biochemical studies will be needed to confirm the proposed CCDC32-AP2 interaction.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Wnt induces FZD5/8 endocytosis and degradation and the involvement of RSPO-ZNRF3/RNF43 and DVL

1. Dong Luo
2. Jing Zheng
3. Shuning Lv
4. Ren Sheng
5. Maorong Chen
6. Xi He
7. Xinjun Zhang

• Curated by eLife

  eLife Assessment

  This study presents important findings demonstrating that the internalization and degradation of FZD5 and FZD8, two of the ten Frizzled proteins, are WNT dependent and do not involve DVL. The evidence supporting the claims of the authors is convincing. This research will be of interest to biologists specializing in Wnt signaling, cancer, and regenerative medicine.

Reviewed by eLife

RNA triggers chronic stress during neuronal aging

1. Kevin Rhine
2. Elle Epstein
3. Natasha M. Carlson
4. Xuezhen Ge
5. Orel Mizrahi
6. Anika Kamat
7. Anita Hermann
8. William R. Brothers
9. John Ravits
10. Eric J. Bennett
11. Gülçin Pekkurnaz
12. Gene W. Yeo

Reviewed by PREreview

Microscopy Nodes: versatile 3D microscopy visualization with Blender

1. Oane Gros
2. Chandni Bhickta
3. Granita Lokaj
4. Yannick Schwab
5. Simone Köhler
6. Niccolò Banterle

Reviewed by Review Commons