Cell Biology

Human airway lineages derived from pluripotent stem cells reveal the epithelial responses to SARS-CoV-2 infection

1. Ruobing Wang
2. Adam J. Hume
3. Mary Lou Beermann
4. Chantelle Simone-Roach
5. Jonathan Lindstrom-Vautrin
6. Jake Le Suer
7. Jessie Huang
8. Judith Olejnik
9. Carlos Villacorta-Martin
10. Esther Bullitt
11. Anne Hinds
12. Mahboobe Ghaedi
13. Stuart Rollins
14. Rhiannon B. Werder
15. Kristine M. Abo
16. Andrew A. Wilson
17. Elke Mühlberger
18. Darrell N. Kotton
19. Finn J. Hawkins

Reviewed by ScreenIT

ER-export and ARFRP1/AP-1–dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication

1. Guy J. Pearson
2. Harriet V. Mears
3. Malgorzata Broncel
4. Ambrosius P. Snijders
5. David L. V. Bauer
6. Jeremy G. Carlton

Reviewed by ScreenIT

Matriptase generates a tissue damage response via promoting Gq signalling, leading to RSK and DUOX activation

1. MA Jiajia
2. Claire A. Scott
3. HO Ying Na
4. Harsha Mahabaleshwar
5. Katherine S. Marsay
6. Changqing Zhang
7. Christopher K. J. Teow
8. NG Ser Sue
9. Weibin Zhang
10. Vinay Tergaonkar
11. Lynda J. Partridge
12. Sudipto Roy
13. Enrique Amaya
14. Tom J. Carney

• Curated by eLife

  Evaluation Summary:

  This manuscript unravels a detailed bipartite signaling mechanism, activation of which results in epithelial inflammation and cell motility. The paper is potentially of broad interest to cancer biologists and epithelial cell biologists. The data generated using the combination of genetic analyses, chemical inhibitors, and state-of-the-art confocal microscopy is of exceptionally high quality and supports the majority of the claims made in this paper.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development

1. Maryam Izadi
2. Eric Seemann
3. Dirk Schlobinski
4. Lukas Schwintzer
5. Britta Qualmann
6. Michael M Kessels

• Curated by eLife

  Evaluation Summary:

  This manuscript is of interest to scientists within the fields of actin cytoskeleton, cellular neurobiology and neurodevelopment. It explores how actin regulators are coordinated to trigger the formation of branches in neuronal dendritic arbor. Experiments are very well performed. Conclusions of the manuscript are convincingly supported by the results, although strict dependence of Cobl and Cobl-like in dendritic branch formation should perhaps be confirmed with additional experiments or tuned down. Results concerning the spatiotemporal relationship between the molecular players involved are more preliminary and few findings already published by the same group in previous articles should be expunged from this manuscript.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Structural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy

1. Miriam S Weber
2. Matthias Eibauer
3. Suganya Sivagurunathan
4. Thomas M Magin
5. Robert D Goldman
6. Ohad Medalia

• Curated by eLife

  Evaluation Summary:

  This work combines 2D and 3D cryo-electron microscopy to show that cellular keratin intermediate filaments have heterogenous diameter, protofilament number and protofilament arrangement. This demonstrates the challenge for future high resolution structure determination of these essential filaments as well as providing the basis for understanding how this heterogeneity facilitates their function.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

A mechanism with severing near barbed ends and annealing explains structure and dynamics of dendritic actin networks

1. Danielle Holz
2. Aaron R Hall
3. Eiji Usukura
4. Sawako Yamashiro
5. Naoki Watanabe
6. Dimitrios Vavylonis

• Curated by eLife

  Evaluation Summary:

  In this work, Holz and colleagues develop a computational stochastic model of lamellipodial actin network growth and turnover to address an unresolved important question: how do these networks remain wide enough, maintain angular order, and actually increase the filament length behind the leading edge? They compare the filament organization and rate of incorporation/detachment of actin subunits with experimental data published in the literature. A main result from this study is that frequent filament fragmentation and annealing are key events in the reorganization of branched actin networks. The paper is well written, contains very thorough and fair literature review, is accurate, well documented. The result is novel and significant.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Kinetochore-bound Mps1 regulates kinetochore–microtubule attachments via Ndc80 phosphorylation

1. Krishna K. Sarangapani
2. Lori B. Koch
3. Christian R. Nelson
4. Charles L. Asbury
5. Sue Biggins

Reviewed by Review Commons

Targeting an anchored phosphatase-deacetylase unit restores renal ciliary homeostasis

1. Janani Gopalan
2. Mitchell H Omar
3. Ankita Roy
4. Nelly M Cruz
5. Jerome Falcone
6. Kiana N Jones
7. Katherine A Forbush
8. Jonathan Himmelfarb
9. Benjamin S Freedman
10. John D Scott

• Curated by eLife

  Evaluation Summary:

  This paper will be of interest to cell biologists interested in understanding processes that regulate the biogenesis of the primary cilium, and to others interested in ciliopathies (ie disorders of the primary cilium). The authors have identified a novel pathway that controls the generation and length of primary cilia and show that its effects are mediated at least in part through its effects on regulating the cytoskeleton. They suggest that this pathway may also have clinical relevance for autosomal dominant polycystic disease, but the data and rationale underlying this part of the study wold need to be strengthened.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

An endosome-associated actin network involved in directed apical plasma membrane growth

1. Luis Daniel Ríos-Barrera
2. Maria Leptin

Reviewed by Review Commons

Glycolytic inhibitor 2-deoxy-d-glucose attenuates SARS-CoV-2 multiplication in host cells and weakens the infective potential of progeny virions

1. Anant Narayan Bhatt
2. Abhishek Kumar
3. Yogesh Rai
4. Neeraj Kumari
5. Dhiviya Vedagiri
6. Krishnan H. Harshan
7. Vijayakumar Chinnadurai
8. Sudhir Chandna

Reviewed by ScreenIT