Cell Biology

ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming

1. José Hélio Costa
2. Gunasekaran Mohanapriya
3. Revuru Bharadwaj
4. Carlos Noceda
5. Karine Leitão Lima Thiers
6. Shahid Aziz
7. Shivani Srivastava
8. Manuela Oliveira
9. Kapuganti Jagadis Gupta
10. Aprajita Kumari
11. Debabrata Sircar
12. Sarma Rajeev Kumar
13. Arvind Achra
14. Ramalingam Sathishkumar
15. Alok Adholeya
16. Birgit Arnholdt-Schmitt

Reviewed by ScreenIT

A PX-BAR protein Mvp1/SNX8 and a dynamin-like GTPase Vps1 drive endosomal recycling

1. Sho W Suzuki
2. Akihiko Oishi
3. Nadia Nikulin
4. Jeff R Jorgensen
5. Matthew G Baile
6. Scott D Emr

• Curated by eLife

  Evaluation Summary:

  This paper will be of broad interest to the vesicle trafficking field, as it defines how an evolutionarily conserved SNX-BAR protein (Mvp1) sorts cargo proteins into membrane tubules emanating from the endosome and recruits a dynamin-like "pinchase" to release the tubule so cargo can move to the Golgi complex.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

The cryptic gonadotropin-releasing hormone neuronal system of human basal ganglia

1. Katalin Skrapits
2. Miklós Sárvári
3. Imre Farkas
4. Balázs Göcz
5. Szabolcs Takács
6. Éva Rumpler
7. Viktória Váczi
8. Csaba Vastagh
9. Gergely Rácz
10. András Matolcsy
11. Norbert Solymosi
12. Szilárd Póliska
13. Blanka Tóth
14. Ferenc Erdélyi
15. Gábor Szabó
16. Michael D Culler
17. Cecile Allet
18. Ludovica Cotellessa
19. Vincent Prévot
20. Paolo Giacobini
21. Erik Hrabovszky

• Curated by eLife

  Evaluation Summary:

  This multifaceted study focuses on neurons in the brain that produce a small peptide molecule known as GnRH, which is central to reproduction in its role as the releasing hormone for gonadotropins from the anterior pituitary. The findings will be of interest to scientists interested in the role of neuropeptides in determining normal brain function and the onset of neurodegenerative disorders. The authors provide a detailed anatomical and molecular characterization of a large, previously unnoticed population of GnRH neurons, located in basal ganglia (mainly putamen) in humans, which diverge from the population of GnRH neurons regulating the pituitary, in number (much larger), morphology and, possibly, origin (not from olfactory placode). Moreover, the study rekindles the idea that GnRH producing neurons in other regions of the brain outside the hypothalamus may be involved in neural processes unrelated to reproduction such as locomotion and decision making.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Locating macromolecular assemblies in cells by 2D template matching with cisTEM

1. Bronwyn A Lucas
2. Benjamin A Himes
3. Liang Xue
4. Timothy Grant
5. Julia Mahamid
6. Nikolaus Grigorieff

• Curated by eLife

  Evaluation Summary:

  Lucas, Himes et al. present multiple practical improvements to the 2D high-resolution template-matching (2DTM) routine for cryo-EM images originally described by Rickgauer et al., eLife 2017. GPU-acceleration and integration into cisTEM make the approach substantially faster and easier to use than the previous CPU-based Matlab implementation. The strengths and weaknesses of the 2DTM are clearly presented and the comparison with 3DTM is thorough. At present the 2DTM approach is likely only suitable for analysis of large assemblies (e.g., ribosomes, proteasomes,etc.) in situ, but future improvements in microscope hardware and the 2DTM routine itself will likely allow application of this approach to smaller complexes.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

High-content image analysis to study phenotypic heterogeneity in endothelial cell monolayers

1. Francois Chesnais
2. Jonas Hue
3. Errin Roy
4. Marco Branco
5. Ruby Stokes
6. Aize Pellon
7. Juliette Le Caillec
8. Eyad Elbahtety
9. Matteo Battilocchi
10. Davide Danovi
11. Lorenzo Veschini

Reviewed by Review Commons

Fine-tuned repression of Drp1-driven mitochondrial fission primes a ‘stem/progenitor-like state’ to support neoplastic transformation

1. Brian Spurlock
2. Danitra Parker
3. Malay Kumar Basu
4. Anita Hjelmeland
5. Sajina GC
6. Shanrun Liu
7. Gene P Siegal
8. Alan Gunter
9. Aida Moran
10. Kasturi Mitra

• Curated by eLife

  Evaluation Summary:

  This paper is of interest for cell biologists studying mitochondrial fission as well as stem cell biologists studying neoplastic transformation. The work helps to clarify how variable levels of the master regulator of mitochondrial fission can have substantially different effects on gene regulation and mitochondrial network properties. A combination of complementary methods is used to support the key findings although aspects of data analysis and quantification could be improved.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Hybrid In Silico Approach Reveals Novel Inhibitors of Multiple SARS-CoV-2 Variants

1. Sankalp Jain
2. Daniel C. Talley
3. Bolormaa Baljinnyam
4. Jun Choe
5. Quinlin Hanson
6. Wei Zhu
7. Miao Xu
8. Catherine Z. Chen
9. Wei Zheng
10. Xin Hu
11. Min Shen
12. Ganesha Rai
13. Matthew D. Hall
14. Anton Simeonov
15. Alexey V. Zakharov

Reviewed by ScreenIT

Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy

1. Lissenya B. Argueta
2. Lauretta A. Lacko
3. Yaron Bram
4. Takuya Tada
5. Lucia Carrau
6. André Figueiredo Rendeiro
7. Tuo Zhang
8. Skyler Uhl
9. Brienne C. Lubor
10. Vasuretha Chandar
11. Cristianel Gil
12. Wei Zhang
13. Brittany J. Dodson
14. Jeroen Bastiaans
15. Malavika Prabhu
16. Sean Houghton
17. David Redmond
18. Christine M. Salvatore
19. Yawei J. Yang
20. Olivier Elemento
21. Rebecca N. Baergen
22. Benjamin R. tenOever
23. Nathaniel R. Landau
24. Shuibing Chen
25. Robert E. Schwartz
26. Heidi Stuhlmann

Reviewed by ScreenIT

A High-Content Screen for Mucin-1-Reducing Compounds Identifies Fostamatinib as a Candidate for Rapid Repurposing for Acute Lung Injury

1. Maria Kost-Alimova
2. Eriene-Heidi Sidhom
3. Abhigyan Satyam
4. Brian T. Chamberlain
5. Moran Dvela-Levitt
6. Michelle Melanson
7. Seth L. Alper
8. Jean Santos
9. Juan Gutierrez
10. Ayshwarya Subramanian
11. Patrick J. Byrne
12. Elizabeth Grinkevich
13. Estefanía Reyes-Bricio
14. Choah Kim
15. Abbe R. Clark
16. Andrew J.B. Watts
17. Rebecca Thompson
18. Jamie Marshall
19. Juan Lorenzo Pablo
20. Juliana Coraor
21. Julie Roignot
22. Katherine A. Vernon
23. Keith Keller
24. Alissa Campbell
25. Maheswarareddy Emani
26. Matthew Racette
27. Silvana Bazua-Valenti
28. Valeria Padovano
29. Astrid Weins
30. Stephen P. McAdoo
31. Frederick W.K. Tam
32. Luciene Ronco
33. Florence Wagner
34. George C. Tsokos
35. Jillian L. Shaw
36. Anna Greka

Reviewed by preLights, ScreenIT

Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx)

1. Hugo GJ Damstra
2. Boaz Mohar
3. Mark Eddison
4. Anna Akhmanova
5. Lukas C Kapitein
6. Paul W Tillberg

• Curated by eLife

  Evaluation Summary:

  This manuscript reports a robust and well-characterized expansion method that achieves 10X expansion with a single expansion step using a simple, easy-to-use protocol. The new protocol leads to an enabling methodology for super-resolution imaging of various sub-cellular structures and organelles and is likely to have a high impact.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife, preLights