Cell Biology

Long-range migration of centrioles to the apical surface of the olfactory epithelium

1. Kaitlin Ching
2. Jennifer T Wang
3. Tim Stearns

• Curated by eLife

  Evaluation Summary:

  Analyzing the long-distance migration of centrioles to the dendrite tip of multicilated olfactory neurons in mice, Ching et al. use expansion microscopy to show that centrioles migrate as clusters, which mature as they reach the apical surface. The super-resolution data are impressive and the claims are generally supported by the data. Although the manuscript is largely descriptive, it is an important addition to the field, and will be of broad interest to cell biologists.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife, preLights

Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes

1. Sai Srinivas Panapakkam Giridharan
2. Guangming Luo
3. Pilar Rivero-Rios
4. Noah Steinfeld
5. Helene Tronchere
6. Amika Singla
7. Ezra Burstein
8. Daniel D Billadeau
9. Michael A Sutton
10. Lois S Weisman

• Curated by eLife

  Evaluation Summary:

  The authors investigate the role of the PI3P 5-kinase protein (PIKfyve) in endosome to cell surface recycling. They report that PIKfyve function is necessary for cell migration and endsomal recycling of integrin proteins via the SNX17-Retriever pathway. The findings will be of interest to the endosomal research community.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

An altered metabolism in leukocytes showing in vitro igG memory from SARS-CoV-2-infected patients

1. G. Fanelli
2. F. Gevi
3. G. Zarletti
4. M. Tiberi
5. V. De Molfetta
6. G. Scapigliati
7. A.M. Timperio

Reviewed by ScreenIT

Upregulated flotillins and sphingosine kinase 2 derail AXL vesicular traffic to promote epithelial-mesenchymal transition

1. Mallory Genest
2. Franck Comunale
3. Damien Planchon
4. Pauline Govindin
5. Dune Noly
6. Sophie Vacher
7. Ivan Bièche
8. Bruno Robert
9. Himanshu Malhotra
10. Andreas Schoenit
11. Liubov A. Tashireva
12. Josefina Casas
13. Cécile Gauthier-Rouvière
14. Stéphane Bodin

Reviewed by Review Commons

Differential interferon-α subtype immune signatures suppress SARS-CoV-2 infection

1. J. Schuhenn
2. T.L. Meister
3. D. Todt
4. T. Bracht
5. K. Schork
6. J.-N. Billaud
7. C. Elsner
8. N Heinen
9. Z. Karakoese
10. S. Haid
11. S. Kumar
12. L. Brunotte
13. M. Eisenacher
14. J. Chen
15. Z Yuan
16. T. Pietschmann
17. B. Wiegmann
18. H. Beckert
19. C. Taube
20. VTK. Le-Trilling
21. M. Trilling
22. A. Krawczyk
23. S. Ludwig
24. B. Sitek
25. E. Steinmann
26. U. Dittmer
27. K. Sutter
28. S. Pfaender

Reviewed by ScreenIT

A  conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation

1. Weichao Zhang
2. Xi Yang
3. Liang Chen
4. Yun-Yu Liu
5. Varsha Venkatarangan
6. Lucas Reist
7. Phyllis Hanson
8. Haoxing Xu
9. Yanzhuang Wang
10. Ming Li

Reviewed by Review Commons

Protective mitochondrial fission induced by stress-responsive protein GJA1-20k

1. Daisuke Shimura
2. Esther Nuebel
3. Rachel Baum
4. Steven E Valdez
5. Shaohua Xiao
6. Junco S Warren
7. Joseph A Palatinus
8. TingTing Hong
9. Jared Rutter
10. Robin M Shaw

• Curated by eLife

  Evaluation Summary:

  This study identifies a cardioprotective factor, GJA1-20k (a truncated form of Cx43), which appears to confer protection against ischemia/reperfusion (I/R) injury via promotion of mitochondrial fission. This finding is particularly interesting given that hyperfission is generally thought of as an index of toxicity in I/R or hypoxic injury. I/R lesion size in a GJA1 heterozygous mutant mouse is strikingly exacerbated compared to control animals, providing strong in vivo evidence supporting a role for this factor in protection from I/R. However, while the findings are interesting and novel, key results require additional experimental support, including to address the lack of key control data, and a significant revision will be necessary to address these issues.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Immunolocalization studies of vimentin and ACE2 on the surface of cells exposed to SARS-CoV-2 Spike proteins

1. Vasiliki Lalioti
2. Silvia González-Sanz
3. Irene Lois-Bermejo
4. Patricia González-Jiménez
5. Álvaro Viedma-Poyatos
6. Andrea Merino
7. María A. Pajares
8. Dolores Pérez-Sala

Reviewed by ScreenIT

Design principles of the ESCRT-III Vps24-Vps2 module

1. Sudeep Banjade
2. Yousuf H Shah
3. Shaogeng Tang
4. Scott D Emr

• Curated by eLife

  Evaluation Summary:

  Different ESCRT-III subunits share sequence similarity but have been characterized in distinct conformations and perform distinct roles in the polymerization process that is central to ESCRT membrane fission pathways. Here it is shown that mutations in one ESCRT-III subunit can compensate for loss of a different subunit by encoding the specialized roles of both subunits within one polypeptide. These findings will be of interest to investigators studying ESCRT pathway mechanisms and those more generally interested in the adaptability of protein sequences and functions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion

1. Haining Zhong
2. Cesar C Ceballos
3. Crystian I Massengill
4. Michael A Muniak
5. Lei Ma
6. Maozhen Qin
7. Stefanie Kaech Petrie
8. Tianyi Mao

• Curated by eLife

  Evaluation Summary:

  The reviewers found your description of the new method interesting and potentially useful for the field. They raised concerns about fusion protein's functionality and reagent accessibility among other technical questions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife