Cell Biology

Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosis

1. Christopher J Brereton
2. Liudi Yao
3. Elizabeth R Davies
4. Yilu Zhou
5. Milica Vukmirovic
6. Joseph A Bell
7. Siyuan Wang
8. Robert A Ridley
9. Lareb SN Dean
10. Orestis G Andriotis
11. Franco Conforti
12. Lennart Brewitz
13. Soran Mohammed
14. Timothy Wallis
15. Ali Tavassoli
16. Rob M Ewing
17. Aiman Alzetani
18. Benjamin G Marshall
19. Sophie V Fletcher
20. Philipp J Thurner
21. Aurelie Fabre
22. Naftali Kaminski
23. Luca Richeldi
24. Atul Bhaskar
25. Christopher J Schofield
26. Matthew Loxham
27. Donna E Davies
28. Yihua Wang
29. Mark G Jones

• Curated by eLife

  Evaluation Summary:

  This study builds upon the previous original work of the authors by identifying a pathway that regulates collagen nanostructure and stiffness in lung fibrosis and demonstrating that this pathway it is independent of pathways regulating collagen synthesis. The analysis performed to validate the specificity of experimental findings and the demonstration that HIF activation is required for the increased tissue stiffness associated with fibrosis are elegant and convincing. With some additional clarifications and controls, this manuscript will be of broad interest to researchers interested in lung biology and especially to those focused on the pathogenesis and therapy of lung fibrosis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Ectocytosis prevents accumulation of ciliary cargo in C. elegans sensory neurons

1. Adria Razzauti
2. Patrick Laurent

• Curated by eLife

  Evaluation Summary:

  Razzauti and Laurent investigate the formation of extracellular vesicles (EVs) by the cilia of C. elegans sensory neurons and the potential functions of this process. Consistent with previous findings, they show that EVs can be released from two distinct sites of the cilium; further, they show that several different classes of sensory neurons can produce EVs, that these can be taken up by a neighboring glial cell, and that this process may be important for the morphology and function of ciliated sensory neurons. However, it remains unclear whether these phenomena may be a consequence of the experimental system (ciliary protein overexpression); additionally, the link between EV uptake by glia and maintenance of neuronal structure and function is not convincingly established.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Processing of the ribosomal ubiquitin-like fusion protein FUBI-eS30/FAU is required for 40S maturation and depends on USP36

1. Jasmin van den Heuvel
2. Caroline Ashiono
3. Ludovic C Gillet
4. Kerstin Dörner
5. Emanuel Wyler
6. Ivo Zemp
7. Ulrike Kutay

• Curated by eLife

  Evaluation Summary:

  This study has discovered deubiquitinase USP36 as the enzyme that processes FAU, a ribosomal protein precursor comprised of a fusion between ubiquitin-like protein FUBI and the ribosomal protein eS30. This is an important advance because correct processing is crucial for biogenesis of the 40S ribosomal subunit. Knowing the identity of the processing enzyme now opens this step in ribosome biogenesis to molecular and mechanistic analysis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

PLEKHA5, PLEKHA6, and PLEKHA7 bind to PDZD11 to target the Menkes ATPase ATP7A to the cell periphery and regulate copper homeostasis

1. Sophie Sluysmans
2. Isabelle Méan
3. Tong Xiao
4. Amina Boukhatemi
5. Flavio Ferreira
6. Lionel Jond
7. Annick Mutero
8. Christopher J. Chang
9. Sandra Citi

Reviewed by Review Commons

Cyclin F drives proliferation through SCF-dependent degradation of the retinoblastoma-like tumor suppressor p130/RBL2

1. Taylor P Enrico
2. Wayne Stallaert
3. Elizaveta T Wick
4. Peter Ngoi
5. Xianxi Wang
6. Seth M Rubin
7. Nicholas G Brown
8. Jeremy E Purvis
9. Michael J Emanuele

• Curated by eLife

  Evaluation Summary:

  The identification of p130 as a substrate of cyclin F adds a new level of understanding about the role of this ubiquitin ligase in cell cycle control. While much of the data are string and of interest, several concerns need to be addressed with the inclusion of new experimental data. This work will be of interest to researchers in the fields of cell cycle and cancer.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

GIV/Girdin, a non-receptor modulator for Gαi/s, regulates spatiotemporal signaling during sperm capacitation and is required for male fertility

1. Sequoyah Reynoso
2. Vanessa Castillo
3. Gajanan Dattatray Katkar
4. Inmaculada Lopez-Sanchez
5. Sahar Taheri
6. Celia Espinoza
7. Cristina Rohena
8. Debashis Sahoo
9. Pascal Gagneux
10. Pradipta Ghosh

• Curated by eLife

  Evaluation Summary:

  This work is of interest to the field of reproduction. Prior to fertilization, spermatozoa undergo a series of morphological and biochemical changes to become fertilization competent, driven by a rapid and poorly understood signaling cascade, culminating in the acrosome reaction. This latter reaction releases to the outside components from a vesicle, the acrosome, in the spermatozoan head and transforms the head plasma membrane so that sperm can fuse with the egg. The work shows that a G protein modulator GIV/Girdin, influences sperm motility and the acrosome reaction. In so doing it is important for fertilization and is one more strategy to control untimely acrosome reaction. The proposed mechanism is well supported by a variety of different experimental approaches.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Integrative Multi-Omics Landscape of Non-Structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses

1. Ruona Shi
2. Zhenhuan Feng
3. Xiaofei Zhang

Reviewed by ScreenIT

Pak1 kinase controls cell shape through ribonucleoprotein granules

1. Joseph O Magliozzi
2. James B Moseley

• Curated by eLife

  Evaluation Summary:

  This paper is of interest to cell biologists studying the signal transduction regulation of RNP granule assembly and its effects on cell growth. The work provides insight into the novel role of Pak1 kinase in the control of mRNA binding protein Sts5 and determines that Pak1 colocalizes with Processing (P) bodies during starvation and has function in P body dissolution after refeeding. Overall, the data are well presented, and support previous known findings.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Variable susceptibility of intestinal organoid–derived monolayers to SARS-CoV-2 infection

1. Kyung Ku Jang
2. Maria E. Kaczmarek
3. Simone Dallari
4. Ying-Han Chen
5. Takuya Tada
6. Jordan Axelrad
7. Nathaniel R. Landau
8. Kenneth A. Stapleford
9. Ken Cadwell

Reviewed by Rapid Reviews Infectious Diseases, ScreenIT

Ultrastructural analysis of nasopharyngeal epithelial cells from patients with SARS-CoV-2 infection

1. Karina Lidianne Alcântara Saraiva
2. Luydson Richardson Silva Vasconcelos
3. Matheus Filgueira Bezerra
4. Rodrigo Moraes Loyo Arcoverde
5. Sinval Pinto Brandão-Filho
6. Constância Flávia Junqueira Ayres
7. Regina Célia Bressan Queiroz de Figueiredo
8. Antonio Pereira-Neves

Reviewed by ScreenIT