Cell Biology

  1. Visualizing molecules of functional human profilin

    1. Morgan L Pimm
    2. Xinbei Liu
    3. Farzana Tuli
    4. Jennifer Heritz
    5. Ashley Lojko
    6. Jessica L Henty-Ridilla

    • Curated by eLife

      Evaluation Summary:

      This paper will be of interest to a broad audience of cell biologists and biochemists who study the cytoskeleton. It reports the development and rigorous characterization of a fully functional, fluorescently labeled version of profilin that can be used to visualize profilin's dynamic interactions in live cells. Owing to profilin's dual functions in regulating actin and microtubule assembly, this technological development will be a useful tool for a wide range of studies aimed at understanding the role of the cytoskeleton in driving fundamental cellular processes.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife, ASAPbio crowd review

    4 evaluationsAppears in 4 listsLatest version Latest activity

  2. Phagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis

    1. Daan Vorselen
    2. Sarah R Barger
    3. Yifan Wang
    4. Wei Cai
    5. Julie A Theriot
    6. Nils C Gauthier
    7. Mira Krendel

    • Curated by eLife

      Evaluation Summary:

      This study is of great interest to cell biologists studying phagocytosis. The work describes a new method for studying phagocytosis; the engulfment of large cargos such as pathogens by the immune system. With this new method, they describe a mechanical force at the rim of the phagocytic cup that function like teeth. This work will advance the field in a new direction.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity

  3. Fluidics system for resolving concentration-dependent effects of dissolved gases on tissue metabolism

    1. Varun Kamat
    2. Brian M Robbings
    3. Seung-Ryoung Jung
    4. John Kelly
    5. James B Hurley
    6. Kenneth P Bube
    7. Ian R Sweet

    • Curated by eLife

      Evaluation Summary:

      This paper presents a flow method for measuring the effects of dissolved gases on tissues while having control over tissue concentration. Working with gases can be challenging. The improvements reported here incorporate technology that allows for metabolic characterization of mammalian tissues while precisely controlling the concentration of abundant gases (e.g., oxygen), as well as trace gases (e.g., hydrogen sulfide). The authors utilize their technology to investigate the metabolic impacts of dissolved hydrogen sulfide, at physiological concentrations. This method should be a powerful tool for the field and enable further experimentation.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  4. Arginine-vasopressin mediates counter-regulatory glucagon release and is diminished in type 1 diabetes

    1. Angela Kim
    2. Jakob G Knudsen
    3. Joseph C Madara
    4. Anna Benrick
    5. Thomas G Hill
    6. Lina Abdul Kadir
    7. Joely A Kellard
    8. Lisa Mellander
    9. Caroline Miranda
    10. Haopeng Lin
    11. Timothy James
    12. Kinga Suba
    13. Aliya F Spigelman
    14. Yanling Wu
    15. Patrick E MacDonald
    16. Ingrid Wernstedt Asterholm
    17. Tore Magnussen
    18. Mikkel Christensen
    19. Tina Vilsbøll
    20. Victoria Salem
    21. Filip K Knop
    22. Patrik Rorsman
    23. Bradford B Lowell
    24. Linford JB Briant

    • Curated by eLife

      Evaluation Summary:

      Although the cellular and molecular mechanisms for insulin secretion regulation are relatively well defined, the precise control of glucagon secretion remains poorly understood. This paper is an elegant and thorough investigation into the role of Arginine-vasopressin (AVP) in glucagon secretion. It is known that AVP is a robust activator of calcium response in pancreatic alpha cells leading to glucagon release. The physiological relevance and regulation of this AVP-induced glucagon secretion is unclear. This manuscript goes a long way in closing this gap.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  5. Local regulation of extracellular vesicle traffic by the synaptic endocytic machinery

    1. Cassandra R. Blanchette
    2. Amy L. Scalera
    3. Kathryn P. Harris
    4. Zechuan Zhao
    5. Erica C. Dresselhaus
    6. Kate Koles
    7. Anna Yeh
    8. Julia K. Apiki
    9. Bryan A. Stewart
    10. Avital A. Rodal

    Reviewed by ASAPbio crowd review

    2 evaluationsAppears in 2 listsLatest version Latest activity

  6. Determinants shaping the nanoscale architecture of the mouse rod outer segment

    1. Matthias Pöge
    2. Julia Mahamid
    3. Sanae S Imanishi
    4. Jürgen M Plitzko
    5. Krzysztof Palczewski
    6. Wolfgang Baumeister

    • Curated by eLife

      Evaluation Summary:

      Pöge at al. present a study of the rod outer segment (ROS). These are specialised cilia of rod photoreceptor cells, essential for sensing light cues and initiating the vision process. The authors apply cryo-FIB milling to generate highly preserved rod samples and report high-quality cryo-tomographic data providing new insights into the ultrastructure of the ROS. The work reveals potential molecular scaffolds both in the lumen of the membrane stacks and on the surface of the stack providing the structural basis for ROS crucial ordered ultrastructure. The data presented here will be highly valuable for the field of phototransduction.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife

    5 evaluationsAppears in 2 listsLatest version Latest activity

  7. Broad ultra-potent neutralization of SARS-CoV-2 variants by monoclonal antibodies specific to the tip of RBD

    1. Hang Ma
    2. Yingying Guo
    3. Haoneng Tang
    4. Chien-Te K. Tseng
    5. Lei Wang
    6. Huifang Zong
    7. Zhenyu Wang
    8. Yang He
    9. Yunsong Chang
    10. Shusheng Wang
    11. Haiqiu Huang
    12. Yong Ke
    13. Yunsheng Yuan
    14. Mingyuan Wu
    15. Yuanyuan Zhang
    16. Aleksandra Drelich
    17. Kempaiah Rayavara Kempaiah
    18. Bi-Hung Peng
    19. Ailin Wang
    20. Kaiyong Yang
    21. Haiyang Yin
    22. Junjun Liu
    23. Yali Yue
    24. Wenbo Xu
    25. Shuangli Zhu
    26. Tianjiao Ji
    27. Xiaoju Zhang
    28. Ziqi Wang
    29. Gang Li
    30. Guangchun Liu
    31. Jingjing Song
    32. Lingling Mu
    33. ZongShang Xiang
    34. Zhangyi Song
    35. Hua Chen
    36. Yanlin Bian
    37. Baohong Zhang
    38. Hui Chen
    39. Jiawei Zhang
    40. Yunji Liao
    41. Li Zhang
    42. Li Yang
    43. Yi Chen
    44. John Gilly
    45. Xiaodong Xiao
    46. Lei Han
    47. Hua Jiang
    48. Yueqing Xie
    49. Qiang Zhou
    50. Jianwei Zhu

    Reviewed by ScreenIT

    1 evaluationAppears in 1 listLatest version Latest activity

  8. Growth-dependent signals drive an increase in early G1 cyclin concentration to link cell cycle entry with cell growth

    1. Robert A Sommer
    2. Jerry T DeWitt
    3. Raymond Tan
    4. Douglas R Kellogg

    • Curated by eLife

      Evaluation Summary:

      This paper examines how cells control their size and will be interesting to scientists studying sizing mechanisms throughout biology. Using yeast cells as a model system, the authors show that an activator of the cell division cycle accumulates as cells grow until a threshold level of activator is achieved. The experiments are performed well, and the high-quality data will be useful for others in the field studying this signaling pathway.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity

  9. Quantitative analysis of nuclear pore complex organization in Schizosaccharomyces pombe

    1. Joseph M Varberg
    2. Jay R Unruh
    3. Andrew J Bestul
    4. Azqa A Khan
    5. Sue L Jaspersen

    Reviewed by ASAPbio crowd review

    1 evaluationAppears in 2 listsLatest version Latest activity

  10. Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection

    1. Martin Scherzer
    2. Fosco Giordano
    3. Maria Solé Ferran
    4. Lena Ström

    Reviewed by Review Commons

    4 evaluationsAppears in 1 listLatest version Latest activity
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