Cell Biology

Mitoxantrone modulates a glycosaminoglycan-spike complex to inhibit SARS-CoV-2 infection

1. Qi Zhang
2. Peter Radvak
3. Juhyung Lee
4. Yue Xu
5. Vivian Cao-Dao
6. Miao Xu
7. Wei Zheng
8. Catherine Z. Chen
9. Hang Xie
10. Yihong Ye

Reviewed by ScreenIT

Increased mTOR signaling, impaired autophagic flux and cell-to-cell viral transmission are hallmarks of SARS-CoV-2 infection

1. Grazielle Celeste Maktura
2. Thomaz Luscher Dias
3. Érika Pereira Zambalde
4. Bianca Brenha
5. Mariene R. Amorim
6. Luana Nunes Santos
7. Lucas Buscaratti
8. João Gabriel de Angeli Elston
9. Cynthia Mara
10. Mariana Camargo Silva Mancini
11. Isadora Carolina Betim Pavan
12. Daniel A. Toledo-Teixeira
13. Karina Bispo-dos-Santos
14. Pierina L. Parise
15. Stefanie Primon Muraro
16. Gabriela Fabiano de Souza
17. Ana Paula Morelli
18. Luiz Guilherme Salvino da Silva
19. Ícaro Maia Santos de Castro
20. Guilherme O. Barbosa
21. Raissa G. Ludwig
22. Thiago L. Knittel
23. Tatiana D. Saccon
24. Marcelo A. Mori
25. Fabiana Granja
26. Hernandes F. Carvalho
27. Luis Lamberti Pinto da Silva
28. Helder I. Nakaya
29. Jose Luiz Proenca-Modena
30. Fernando Moreira Simabuco
31. Henrique Marques-Souza

Reviewed by ScreenIT

Aging is associated with increased brain iron through cortex-derived hepcidin expression

1. Tatsuya Sato
2. Jason Solomon Shapiro
3. Hsiang-Chun Chang
4. Richard A Miller
5. Hossein Ardehali

• Curated by eLife

  Evaluation Summary:

  Accumulation of redox-active iron in the brain is a significant cause of neurotoxicity in neurodegenerative diseases of old age. Thus, this manuscript could be of interest to neuroscientists, iron biologists, and those studying mechanisms of aging as it provides some new mechanistic insight on the role of age-related increases in hepcidin in brain iron accumulation. The current study demonstrates increased cytosolic and mitochondrial non-heme iron only in the aging brain, increased local hepcidin expression, and decreased levels of FPN1, together supporting a hypothesis that local brain hepcidin sequesters iron in neuronal cells and is associated with aging.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Autophagy–mediated plasma membrane removal promotes the formation of epithelial syncytia

1. Parisa Kakanj
2. Sourabh Bhide
3. Bernard Moussian
4. Maria Leptin

Reviewed by Review Commons

Pathogenic mutations in the chromokinesin KIF22 disrupt anaphase chromosome segregation

1. Alex F Thompson
2. Patrick R Blackburn
3. Noah S Arons
4. Sarah N Stevens
5. Dusica Babovic-Vuksanovic
6. Jane B Lian
7. Eric W Klee
8. Jason Stumpff

Reviewed by ASAPbio crowd review

Regulation of the COPII secretory machinery via focal adhesions and extracellular matrix signaling

1. Juan Jung
2. Muzamil Majid Khan
3. Jonathan Landry
4. Aliaksandr Halavatyi
5. Pedro Machado
6. Miriam Reiss
7. Rainer Pepperkok

Reviewed by Review Commons

Transferrin receptor 1-mediated iron uptake regulates bone mass in mice via osteoclast mitochondria and cytoskeleton

1. Bhaba K Das
2. Lei Wang
3. Toshifumi Fujiwara
4. Jian Zhou
5. Nukhet Aykin-Burns
6. Kimberly J Krager
7. Renny Lan
8. Samuel G Mackintosh
9. Ricky Edmondson
10. Michael L Jennings
11. Xiaofang Wang
12. Jian Q Feng
13. Tomasa Barrientos
14. Jyoti Gogoi
15. Aarthi Kannan
16. Ling Gao
17. Weirong Xing
18. Subburaman Mohan
19. Haibo Zhao

• Curated by eLife

  Evaluation Summary:

  The authors present a very well written manuscript addressing an important unknown in bone homeostasis, aiming to understand the mechanism of iron mediated effects on bone, findings of novel significance that are of interest to basic iron biologists, bone biologists, experts in mitochondrial respiration, and endocrinologists. This is the first study to show that Tfr1 is important for iron uptake in vivo and for proper osteoclast function. Mechanistically, Tfr1-mediated iron uptake is important for mitochondrial function and cytoskeleton organization, which is important for bone resorption. Overall, this study adds important information regarding the role of Tfr1 and iron metabolism in osteoclasts.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

mTOR activation induces endolysosomal remodeling and nonclassical secretion of IL-32 via exosomes in inflammatory reactive astrocytes

1. Kun Leng
2. Brendan Rooney
3. Frank McCarthy
4. Wenlong Xia
5. Indigo V.L. Rose
6. Sophie Bax
7. Marcus Chin
8. Saeed Fathi
9. Kari A. Herrington
10. Manuel Leonetti
11. Aimee Kao
12. Stephen P.J. Fancy
13. Joshua E. Elias
14. Martin Kampmann

Reviewed by ASAPbio crowd review

Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans

1. Ian F Price
2. Hannah L Hertz
3. Benjamin Pastore
4. Jillian Wagner
5. Wen Tang

• Curated by eLife

  Evaluation Summary:

  The authors use proximity labeling and genetic experiments to identify and functionally characterize new components of C. elegans P granules. The conclusions of the paper are well-supported by the data. This work will be of broad interest to developmental biologists, particularly those interested in the formation and function of germ cells.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

The supramolecular landscape of growing human axons

1. Patrick C. Hoffmann
2. Stefano L. Giandomenico
3. Iva Ganeva
4. Michael R. Wozny
5. Magdalena Sutcliffe
6. Wanda Kukulski
7. Madeline A. Lancaster

• Curated by eLife

  Evaluation Summary:

  The authors have elegantly combined two techniques, air-liquid interface cerebral organoid (ALI-CO) with correlative light and electron cryo-microscopy (cryo-CLEM), to study the ultrastructure of developing human axons. The technique presented is useful and the data is of high quality and well presented. With a somewhat stronger demonstration of the molecular resolution achieved and a description of how this technique can be expanded to study other organoids or cellular structures in non-neuronal cells and tissues, this paper will be of broad interest to neuroscientists and those developing cryo-electron tomography methods.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife