Cell Biology

Near-infrared imaging in fission yeast using a genetically encoded phycocyanobilin biosynthesis system

1. Keiichiro Sakai
2. Yohei Kondo
3. Hiroyoshi Fujioka
4. Mako Kamiya
5. Kazuhiro Aoki
6. Yuhei Goto

Reviewed by Review Commons

TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis

1. Zhong-Jiao Jiang
2. Wenping Li
3. Li-Hua Yao
4. Badeia Saed
5. Yan Rao
6. Brian S Grewe
7. Andrea McGinley
8. Kelly Varga
9. Simon Alford
10. Ying S Hu
11. Liang-Wei Gong

• Curated by eLife

  Evaluation Summary:

  Jiang et al. present a study on a novel role of the ion channel TRPM7 in the regulated release of neurotransmitters and hormones. Their analysis focused on TRPM7-deficient chromaffin cells and neurons, which they studied using electrophysiological and imaging techniques. The data indicate that TRPM7 is activated during the compensatory endocytotis process that follows secretory vesicle fusion. This TRPM7 activation increases Ca2+ influx into the cytosol during endocytosis, which boosts endocytosis and reduces the tendency of synapses to progressively fatigue in phases of prolonged stimulation. Multiple aspects of the present findings are novel, interesting, and important - they provide important new insights into the mechanisms by which transmitter and hormone release is fine-tuned. The mechanism of how secretory vesicles, and in particular synaptic vesicles, recycle is important to understand, as impairment in vesicle cycling in turn impairs synaptic transmission, and therefore brain function. However, there are several issues that remain to be addressed before the present data fully justify the conclusions put forward by the authors.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

The spike protein of SARS-CoV-2 induces endothelial inflammation through integrin α5β1 and NF-κB signaling

1. Juan Pablo Robles
2. Magdalena Zamora
3. Elva Adan-Castro
4. Lourdes Siqueiros-Marquez
5. Gonzalo Martinez de la Escalera
6. Carmen Clapp

Reviewed by ScreenIT

3D cell neighbour dynamics in growing pseudostratified epithelia

1. Harold Fernando Gómez
2. Mathilde Sabine Dumond
3. Leonie Hodel
4. Roman Vetter
5. Dagmar Iber

• Curated by eLife

  Evaluation Summary:

  The authors measure the three-dimensional organization within an epithelial cell monolayer and find that cell neighbors change frequently along the apicobasal axis. State-of-the-art image analysis convincingly justifies correlation, though not causation, between epithelial cell packing and nuclear position. With some stronger theoretical arguments to back up the claims made, this paper will be of interest to scientists studying tissue mechanics and packing of cells in epithelial tissues.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast

1. Olivia X. Ma
2. Wen Guan Chong
3. Joy K. E. Lee
4. Shujun Cai
5. C. Alistair Siebert
6. Andrew Howe
7. Peijun Zhang
8. Jian Shi
9. Uttam Surana
10. Lu Gan

Reviewed by Review Commons

Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures

1. Shannon Rausser
2. Caroline Trumpff
3. Marlon A McGill
4. Alex Junker
5. Wei Wang
6. Siu-Hong Ho
7. Anika Mitchell
8. Kalpita R Karan
9. Catherine Monk
10. Suzanne C Segerstrom
11. Rebecca G Reed
12. Martin Picard

• Curated by eLife

  Evaluation Summary:

  This paper is of broad interest to those in the field of immunometabolism, a field which has largely used the mouse as an experimental system. The descriptive work is the first of its kind to demonstrate important aspects of biological variability and hidden aspects of mitochondrial function in human immune cells.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites

1. Caroline S Simon
2. Charlotta Funaya
3. Johanna Bauer
4. Yannik Voβ
5. Marta Machado
6. Alexander Penning
7. Darius Klaschka
8. Marek Cyrklaff
9. Juyeop Kim
10. Markus Ganter
11. Julien Guizetti

Reviewed by Review Commons

Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization

1. Pavan Choppakatla
2. Bastiaan Dekker
3. Erin E Cutts
4. Alessandro Vannini
5. Job Dekker
6. Hironori Funabiki

• Curated by eLife

  Evaluation Summary:

  This paper will be of interest to scientists within the field of chromosome biology. The authors take advantage of the Xenopus egg cell free system and combine classical morphological analyses by immunofluorescence with chromosome conformation (Hi-C) analyses to elucidate the contribution of linker histone H1 to mitotic chromosome organization. The authors find that linker histone H1 limits the association of condensin and topoisomerase II to control chromosome length.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration

1. Shuhao Lin
2. Daiki Hirayama
3. Gembu Maryu
4. Kimiya Matsuda
5. Naoya Hino
6. Eriko Deguchi
7. Kazuhiro Aoki
8. Ryo Iwamoto
9. Kenta Terai
10. Michiyuki Matsuda

Reviewed by Review Commons

An untargeted metabolomic approach to identify antiviral defense mechanisms in memory leukocytes secreting in vitro IgG anti-SARS-Cov-2

1. Gevi Federica
2. Fanelli Giuseppina
3. Lelli Veronica
4. Zarletti Gianpaolo
5. Tiberi Massimo
6. De Molfetta Veronica
7. Scapigliati Giuseppe
8. Timperio Anna Maria

Reviewed by ScreenIT