Cell Biology

Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro

1. Gang Wang
2. Jun Guan
3. Guojun Li
4. Fengtian Wu
5. Qin Yang
6. Chunhong Huang
7. Junwei Shao
8. Lichen Xu
9. Zixuan Guo
10. Qihui Zhou
11. Haihong Zhu
12. Zhi Chen

Reviewed by ScreenIT

Global cellular response to chemical perturbation of PLK4 activity and abnormal centrosome number

1. Johnny M Tkach
2. Reuben Philip
3. Amit Sharma
4. Jonathan Strecker
5. Daniel Durocher
6. Laurence Pelletier

Reviewed by Review Commons

Microtubule rescue at midzone edges promotes overlap stability and prevents spindle collapse during anaphase B

1. Manuel Lera-Ramirez
2. François J Nédélec
3. Phong T Tran

• Curated by eLife

  Evaluation Summary:

  This study carefully quantifies microtubule dynamics during anaphase in the fission yeast S. pombe. The high quality data revealed two new observations: that microtubule rescue occurs preferentially at the edge of the midzone and that microtubule growth speed decreases when the nuclear membrane wraps around the spindle midzone in late anaphase. This sheds new light on the interplay between the nuclear membrane and the midspindle in closed mitosis, and the study will be of interest to cell biologists studying spindle dynamics and mitosis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by ASAPbio crowd review, eLife

Entrectinib—A SARS-CoV-2 Inhibitor in Human Lung Tissue (HLT) Cells

1. Alejandro Peralta-Garcia
2. Mariona Torrens-Fontanals
3. Tomasz Maciej Stepniewski
4. Judith Grau-Expósito
5. David Perea
6. Vikram Ayinampudi
7. Maria Waldhoer
8. Mirjam Zimmermann
9. María J. Buzón
10. Meritxell Genescà
11. Jana Selent

Reviewed by ScreenIT

MTCL2 promotes asymmetric microtubule organization by crosslinking microtubules on the Golgi membrane

1. Risa Matsuoka
2. Masateru Miki
3. Sonoko Mizuno
4. Yurina Ito
5. Chihiro Yamada
6. Atsushi Suzuki

Reviewed by Review Commons

Structural basis for membrane recruitment of ATG16L1 by WIPI2 in autophagy

1. Lisa M Strong
2. Chunmei Chang
3. Julia F Riley
4. C Alexander Boecker
5. Thomas G Flower
6. Cosmo Z Buffalo
7. Xuefeng Ren
8. Andrea KH Stavoe
9. Erika LF Holzbaur
10. James H Hurley

• Curated by eLife

  Evaluation Summary:

  This paper describes the crystal structure of two key components of the autophagy system, the PI3P-binding protein WIPI2d in complex with its interaction region in the hATG8 E3 ligase scaffold component ATG16L1. The paper provides interesting new data and demonstrates the requirements for association of WIPI2d with membranes. Functional studies in cells provide evidence that mutation of residues at the interface for ATG16L1 binding affects function, although additional studies would support loss of function versus a dominant negative effect.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Selective sorting of microRNAs into exosomes by phase-separated YBX1 condensates

1. Xiao-Man Liu
2. Liang Ma
3. Randy Schekman

• Curated by eLife

  Evaluation Summary:

  The ribonucleoprotein YBX1 is required for sorting of miR-233 to exosomes. Here, the authors demonstrate that YBX1 undergoes liquid liquid phase separation (LLPS) both in vitro and in vivo and YBX1 droplets specifically partition miR-233 and mediate its packaging into exosomes. The authors also demonstrate a possible connection between YBX1 condensates and P-bodies during cargo sorting into EVs. Overall, this is an elegant study and with a few additional experiments to clarify the involvement of P bodies, the story will be of broad impact to those interested in LLPS and RNA packaging into exosomes.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Information transfer in mammalian glycan-based communication

1. Felix F Fuchsberger
2. Dongyoon Kim
3. Natalia Baranova
4. Hanka Vrban
5. Marten Kagelmacher
6. Robert Wawrzinek
7. Christoph Rademacher

• Curated by eLife

  Evaluation Summary:

  This manuscript applies the framework of information theory to lectin-glycan signaling modulating the NF-kappaB response. The paper suggests that the information transfer capacity and information flow through the signaling pathway may be affected by a combined action of two distinct receptors having different distributions across a cell population, with possible implications for the immune response. The paper can have an impact on our understanding of signaling through multiple receptors converging on the same output, and will be of interest to experts in cellular signaling, particularly those with interest in innate immune response.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

The extracellular matrix controls stem cell specification and crypt morphology in the developing and adult mouse gut

1. Rana Ramadan
2. Valérie M. Wouters
3. Sanne M. van Neerven
4. Nina E. de Groot
5. Tania Martins Garcia
6. Vanessa Muncan
7. Olivia D. Franklin
8. Michelle Battle
9. Karen Sue Carlson
10. Joshua Leach
11. Owen J. Sansom
12. Olivier Boulard
13. Mathias Chamaillard
14. Louis Vermeulen
15. Jan Paul Medema
16. David J. Huels

Reviewed by Review Commons

Neutrophilic inflammation promotes SARS-CoV-2 infectivity and augments the inflammatory responses in airway epithelial cells

1. BA Calvert
2. EJ Quiroz
3. Z Lorenzana
4. N Doan
5. S Kim
6. CN Senger
7. WD Wallace
8. MP Salomon
9. J Henley
10. AL Ryan

Reviewed by ScreenIT