1. Unveiling Cas8 Dynamics and Regulation within a transposon-encoded Cascade-TniQ Complex

    This article has 4 authors:
    1. Amun C. Patel
    2. Souvik Sinha
    3. Pablo R. Arantes
    4. Giulia Palermo

    Reviewed by PREreview

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  2. Dynamics and activation of membrane-bound B cell receptor assembly

    This article has 4 authors:
    1. Hung N. Do
    2. Mingfei Zhao
    3. S. Munir Alam
    4. S. Gnanakaran

    Reviewed by PREreview

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  3. Dynamic basis of lipopolysaccharide export by LptB2FGC

    This article has 4 authors:
    1. Marina Dajka
    2. Tobias Rath
    3. Nina Morgner
    4. Benesh Joseph
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study provides an important advance in the molecular understanding of the lipopolysaccharide export mechanism and machinery in bacteria. By using advanced spectroscopy approaches, the experiments provide convincing biophysical support for the dynamic behavior of the multisubunit Lpt transport system. This work has implications for understanding bacterial cell envelope biogenesis and developing drugs that target Gram negative pathogens.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 2 listsLatest version Latest activity
  4. Single turnover transient state kinetics reveals processive protein unfolding catalyzed by Escherichia coli ClpB

    This article has 3 authors:
    1. Jaskamaljot Kaur Banwait
    2. Liana Islam
    3. Aaron L Lucius
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study presents the development of a single turnover stopped-flow fluorescence experiment to study the kinetics of substrate unfolding and translocation by the bacterial ClpB disaggregase. Using non-physiological nucleotides to bypass the physiological regulation mechanism of ClpB, the authors convincingly show that the ClpB disaggregase is a processive motor with a slow unfolding step preceding rapid translocation. The results of this analysis are of value for future mechanistic studies on energy-dependent unfolding, degradation, and disaggregation molecular machines.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 1 listLatest version Latest activity
  5. Should I stay or should I go? Spatio-temporal dynamics of bacterial biofilms in confined flows

    This article has 6 authors:
    1. Massinissa Benbelkacem
    2. Gabriel Ramos
    3. Fatima El Garah
    4. Yara Abidine
    5. Christine Roques
    6. Yohan Davit
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study integrates microfluidic experiments and mathematical modeling to investigate how flow dynamics and biofilm growth and detachment influence each other. Using Pseudomonas aeruginosa as a model organism, the study identifies several key effects and stages in biofilm development, albeit with some weaknesses in clearly defining the setup and some of their interpretations. The comparison between experimental results and theoretical models is convincing, providing a robust analysis of the biofilm's behavior under varying flow conditions. The findings will be helpful for researchers working on biofilms and their applications.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  6. Robust optogenetic inhibition with red-light-sensitive anion-conducting channelrhodopsins

    This article has 8 authors:
    1. Johannes Oppermann
    2. Andrey Rozenberg
    3. Thomaz Fabrin
    4. Cristian González-Cabrera
    5. Rafael Parker
    6. Oded Béjà
    7. Matthias Prigge
    8. Peter Hegemann
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study describes the discovery and further engineering of a red light-activated, chloride-conducting Channelrhodopsin (ACR) that could be used to inhibit neuronal activity. The evidence for the spectral confirmation and biophysical characterization of MsACR and raACR, and ion selectivity are solid; however, the evidence supporting the use of the tools in vivo is incomplete and missing proper controls. In addition, benchmarking against other inhibitory tools is somewhat missing. With the in vivo part strengthened, this paper would interest neuroscientists seeking more efficient ways to inhibit neuronal activity.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 1 listLatest version Latest activity
  7. The exchange dynamics of biomolecular condensates

    This article has 7 authors:
    1. Yaojun Zhang
    2. Andrew GT Pyo
    3. Ross Kliegman
    4. Yoyo Jiang
    5. Clifford P Brangwynne
    6. Howard A Stone
    7. Ned S Wingreen
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable contribution studies factors that impact molecular exchange between dense and dilute phases of biomolecular condensates through continuum models and coarse-grained simulations. The authors provide convincing evidence that interfacial resistance can cause molecules to bounce off the interface and limit mixing. Results like these can inform how experimental results in the field of biological condensates are interpreted.

    Reviewed by eLife

    This article has 6 evaluationsAppears in 1 listLatest version Latest activity
  8. The actin cortex acts as a mechanical memory of morphology in confined migrating cells

    This article has 5 authors:
    1. Yohalie Kalukula
    2. Marine Luciano
    3. Guillaume Charras
    4. David B. Brückner
    5. Sylvain Gabriele

    Reviewed by preLights

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  9. Revealing a hidden conducting state by manipulating the intracellular domains in KV10.1 exposes the coupling between two gating mechanisms

    This article has 4 authors:
    1. Reham Abdelaziz
    2. Adam P Tomczak
    3. Andreas Neef
    4. Luis A Pardo
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study examines the role of the interaction between cytoplasmic N- and C-terminal domains in voltage-dependent gating of Kv10.1 channels. The authors suggest that they have identified a hidden open state in Kv10.1 mutant channels, thus providing a window for observing early conformational transitions associated with channel gating. The evidence supporting the major conclusions is solid, but additional work is required to determine the molecular mechanism underlying the observations in this study. Learning the molecular mechanisms could be significant in understanding the gating mechanisms of the KCNH family and will appeal to biophysicists interested in ion channels and physiologists interested in cancer biology.

    Reviewed by eLife

    This article has 12 evaluationsAppears in 2 listsLatest version Latest activity
  10. Stochastic tug-of-war among sarcomeres mediates cardiomyocyte response to environmental stiffness

    This article has 6 authors:
    1. Daniel Haertter
    2. Lara Hauke
    3. Til Driehorst
    4. Kengo Nishi
    5. Wolfram-Hubertus Zimmermann
    6. Christoph F Schmidt
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study provides a valuable characterization of individual sarcomere's contractility and synchrony in spontaneously beating cardiomyocytes as a function of substrate stiffness. The authors, however, provide an incomplete explanation for the observed heterogeneous and stochastic dynamics, so that the work remains mainly descriptive. The work will be of interest to scientists working on muscle biophysics, nonlinear dynamics, and synchronization phenomena in biological systems.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
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