Bioengineering

High-resolution imaging of the osteogenic and angiogenic interface at the site of murine cranial bone defect repair via multiphoton microscopy

1. Kevin Schilling
2. Yuankun Zhai
3. Zhuang Zhou
4. Bin Zhou
5. Edward Brown
6. Xinping Zhang

• Curated by eLife

  eLife assessment

  The authors present very exciting findings on the cranial bone defect repair using cutting-edge multiphoton imaging to study the role of different vessel subtypes and related oxygen and metabolic microenvironments. The study used microscopy to visualize the oxygen distribution and energy metabolism within the defects at different time points during the process of bone healing. This allows one to understand the pathophysiological progressions of bone diseases and regeneration. It will also provide critical information to optimize the therapeutic bone healing and regeneration approach for different clinical situations.

Reviewed by eLife

Translational rapid ultraviolet-excited sectioning tomography for whole-organ multicolor imaging with real-time molecular staining

1. Wentao Yu
2. Lei Kang
3. Victor TC Tsang
4. Yan Zhang
5. Ivy HM Wong
6. Terence TW Wong

• Curated by eLife

  eLife Assessment:

  This paper demonstrates an integrated labeling and block face fluorescence imaging method that enables the rapid evaluation of biological specimens as large as an E18 mouse embryo with single cell resolution. Such capabilities will likely be of great interest to developmental biologists and pathologists. While the approach can be considered a major step forward, additional experimental support is necessary to gauge how quantitative the method is.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

High spatial resolution analysis using automated indentation mapping differentiates biomechanical properties of normal vs. degenerated articular cartilage in mice

1. Anand O Masson
2. Bryce Besler
3. W Brent Edwards
4. Roman J Krawetz

• Curated by eLife

  Evaluation Summary:

  The work presented by Masson et al. highlights experimental approaches using spatial indentation and contrast-enhanced 3-D x-ray imaging to topographically map cartilage thickness in mouse knee joints. This methods described have the potential to impact the field of musculoskeletal biomechanics, especially for researchers using mouse models to study cartilage wear and disease, given the high resolution and sensitivity of the described approaches.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

The mini-IDLE 3D biomimetic culture assay enables interrogation of mechanisms governing muscle stem cell quiescence and niche repopulation

1. Erik Jacques
2. Yinni Kuang
3. Allison P Kann
4. Fabien Le Grand
5. Robert S Krauss
6. Penney M Gilbert

• Curated by eLife

  Evaluation Summary:

  This Methods paper seeks procedures evaluating the balance between muscle cell quiescence and activation. These could well permit investigations of long-standing questions in key areas of muscle function. The latter include the regulation of adult stem cell pool size and functional heterogeneities in this, as well as regulators of muscle quiescence.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Metrics of high cofluctuation and entropy to describe control of cardiac function in the stellate ganglion

1. Nil Z Gurel
2. Koustubh B Sudarshan
3. Joseph Hadaya
4. Alex Karavos
5. Taro Temma
6. Yuichi Hori
7. J Andrew Armour
8. Guy Kember
9. Olujimi A Ajijola

• Curated by eLife

  Evaluation Summary:

  This study will interest basic and clinical scientists and, potentially, device manufacturers interested in the regulation of heart rate in health and disease. A major control of the heart is from the nervous system originating in a neuronal cluster sitting outside the heart called the stellate ganglia. This study has identified the neural code associated with a healthy heart and describes how it changes in disease. Whether the change in code is cause or effect remains equivocal although normalising the code may have valued therapeutic benefit. The study opens the way for sophisticated mimicking of healthy neural code applied to a diseased heart as a potential electroceutical approach.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

SUMOylation of SARS-CoV-2 Nucleocapsid protein enhances its interaction affinity and plays a critical role for its nuclear translocation

1. Vipul Madahar
2. Victor G. J. Rodgers
3. Jiayu Liao

Reviewed by ScreenIT

Potent and pan-neutralization of SARS-CoV-2 variants of concern by DARPins

1. Vikas Chonira
2. Young Do Kwon
3. Jason Gorman
4. James Brett Case
5. Zhiqiang Ku
6. Rudo Simeon
7. Ryan G. Casner
8. Darcy R. Harris
9. Adam S. Olia
10. Tyler Stephens
11. Lawrence Shapiro
12. Hannah Boyd
13. Yaroslav Tsybovsky
14. Florian Krammer
15. Michael S. Diamond
16. Peter D. Kwong
17. Zhiqiang An
18. Zhilei Chen

Reviewed by ScreenIT

Subcutaneous delivery of an antibody against SARS-CoV-2 from a supramolecular hydrogel depot

1. Catherine M. Kasse
2. Anthony C. Yu
3. Abigail E. Powell
4. Gillie A. Roth
5. Celine S. Liong
6. Carolyn K. Jons
7. Awua Buahin
8. Caitlin L. Maikawa
9. Xueting Zhou
10. Sawsan Youssef
11. Jacob E. Glanville
12. Eric A. Appel

Reviewed by ScreenIT

Adaptation‐Proof SARS‐CoV‐2 Vaccine Design

1. Yashavantha L. Vishweshwaraiah
2. Brianna Hnath
3. Brendan Rackley
4. Jian Wang
5. Abhinay Gontu
6. Morgan Chandler
7. Kirill A. Afonin
8. Suresh V. Kuchipudi
9. Neil Christensen
10. Neela H. Yennawar
11. Nikolay V. Dokholyan

Reviewed by ScreenIT

Net-Shaped DNA Nanostructures Designed for Rapid/Sensitive Detection and Potential Inhibition of the SARS-CoV-2 Virus

1. Neha Chauhan
2. Yanyu Xiong
3. Shaokang Ren
4. Abhisek Dwivedy
5. Nicholas Magazine
6. Lifeng Zhou
7. Xiaohe Jin
8. Tianyi Zhang
9. Brian T. Cunningham
10. Sherwood Yao
11. Weishan Huang
12. Xing Wang

Reviewed by ScreenIT