Bioengineering

Directing Cholangiocyte Morphogenesis in Natural Biomaterial Scaffolds

1. Quinton Smith
2. Jennifer Bays
3. Linqing Li
4. Haniyah Shareef
5. Christopher S. Chen
6. Sangeeta N. Bhatia

• Curated by eLife

  Evaluation Summary:

  This paper is of interest for cell biologists, developmental biologists and tissue engineers. The authors identify a combination of natural extracellular matrix and growth factors that enables to grow cholangiocytes as branched three-dimensional ducts in culture. The work is physiologically relevant and represents an interesting step forward in the study of bile duct formation and disease, although the cultured ducts could be characterized more in depth.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Argon plasma-modified bacterial cellulose filters for protection against respiratory pathogens

1. Anna Żywicka
2. Daria Ciecholewska-Juśko
3. Magdalena Charęza
4. Radosław Drozd
5. Peter Sobolewski
6. Adam Junka
7. Selestina Gorgieva
8. Miroslawa El Fray
9. Karol Fijałkowski

Reviewed by ScreenIT

Non-invasive classification of macrophage polarisation by 2P-FLIM and machine learning

1. Nuno GB Neto
2. Sinead A O'Rourke
3. Mimi Zhang
4. Hannah K Fitzgerald
5. Aisling Dunne
6. Michael G Monaghan

• Curated by eLife

  Evaluation Summary:

  Neto et al set out to use Two-Photon FLIM and machine learning to classify macrophages that are polarised along the M1/M2 axis and then subjected to different metabolic stresses classically used to distinguish metabolic strategies of different cell states. Additional information is sought regarding the photophysics of the measurements and if there are an adequate number of photons to fairly compare the three conditions. The work will be of interest to immunologists, physiologists interested in metabolism and engineers looking to translate the findings.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells

1. Matias I Autio
2. Efthymios Motakis
3. Arnaud Perrin
4. Talal Bin Amin
5. Zenia Tiang
6. Dang Vinh Do
7. Jiaxu Wang
8. Joanna Tan
9. Shirley Suet Lee Ding
10. Wei Xuan Tan
11. Chang Jie Mick Lee
12. Adrian Kee Keong Teo
13. Roger SY Foo

Reviewed by Review Commons

Vascular inflammation exposes perivascular cells to SARS-CoV-2 infection

1. Cristiane Miranda Franca
2. Amin Mansoorifar
3. Avathamsa Athirasala
4. Ramesh Subbiah
5. Anthony Tahayeri
6. Luiz Bertassoni

Reviewed by ScreenIT

Genetically Engineered MRI-Trackable Extracellular Vesicles as SARS-CoV-2 Mimetics for Mapping ACE2 Binding In Vivo

1. Andrea Galisova
2. Jiri Zahradnik
3. Hyla Allouche-Arnon
4. Mattia I. Morandi
5. Paula Abou Karam
6. Michal Fisler
7. Ori Avinoam
8. Neta Regev-Rudzki
9. Gideon Schreiber
10. Amnon Bar-Shir

Reviewed by ScreenIT

Artifact-free whole-slide imaging with structured illumination microscopy and Bayesian image reconstruction

1. Karl A Johnson
2. Guy M Hagen

Reviewed by GigaScience

Potent and specific human monoclonal antibodies against SARS-CoV-2 Omicron variant by rapid mRNA immunization of humanized mice

1. Ping Ren
2. Lei Peng
3. Zhenhao Fang
4. Kazushi Suzuki
5. Paul Renauer
6. Qianqian Lin
7. Meizhu Bai
8. Luojia Yang
9. Tongqing Li
10. Paul Clark
11. Daryl Klein
12. Sidi Chen

Reviewed by ScreenIT

Stable nebulization and muco‐trapping properties of regdanvimab/ IN ‐006 support its development as a potent, dose‐saving inhaled therapy for COVID ‐19

1. Morgan D. McSweeney
2. Ian Stewart
3. Zach Richardson
4. Hyunah Kang
5. Yoona Park
6. Cheolmin Kim
7. Karthik Tiruthani
8. Whitney Wolf
9. Alison Schaefer
10. Priya Kumar
11. Harendra Aurora
12. Jeff Hutchins
13. Jong Moon Cho
14. Anthony J. Hickey
15. Soo Young Lee
16. Samuel K. Lai

Reviewed by ScreenIT

Modular capsid decoration boosts adenovirus vaccine-induced humoral immunity against SARS-CoV-2

1. Matthew D.J. Dicks
2. Louisa M. Rose
3. Rebecca A. Russell
4. Lesley A.H. Bowman
5. Carl Graham
6. Jose M. Jimenez-Guardeño
7. Katie J. Doores
8. Michael H. Malim
9. Simon J. Draper
10. Mark Howarth
11. Sumi Biswas

Reviewed by ScreenIT