1. The flexible stalk domain of sTREM2 modulates its interactions with brain-based phospholipids

    This article has 6 authors:
    1. David Saeb
    2. Emma E. Lietzke
    3. Daisy I. Fuchs
    4. Emma C. Aldrich
    5. Kimberley D. Bruce
    6. Kayla G. Sprenger
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This useful manuscript addresses some key molecular mechanisms on the neuroprotective roles of soluble TREM2 in neurodegenerative diseases. The study will advance our understanding of TREM2 mutations, particularly on the damaging effect of known TREM2 mutations, and also provides solid evidence why soluble TREM2 can antagonize Aβ aggregation.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 1 listLatest version Latest activity
  2. An automated microfluidic platform for toxicity testing based on Caenorhabditis elegans

    This article has 10 authors:
    1. Jiaying Wu
    2. Jing Xi
    3. Zhouhai Zhu
    4. Wei Zhao
    5. Qiyuan Peng
    6. Chunbo Liu
    7. Ziqian Wan
    8. Yiyi Cao
    9. Xiang Chen
    10. Yang Luan

    Reviewed by Arcadia Science

    This article has 11 evaluationsAppears in 1 listLatest version Latest activity
  3. Modular DNA Barcoding of Nanobodies Enables Multiplexed in situ Protein Imaging and High-throughput Biomolecule Detection

    This article has 6 authors:
    1. Shilin Zhong
    2. Ruiyu Wang
    3. Xinwei Gao
    4. Qingchun Guo
    5. Rui Lin
    6. Minmin Luo
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This fundamental manuscript presents a practical modification of the orthogonal hybridization chain reaction (HCR) technique, a promising yet underutilized method with broad potential for future applications across various fields. The authors advance this technique by integrating peptide ligation technology and nanobody-based antibody mimetics-cost-effective and scalable alternatives to conventional antibodies-into a DNA-immunoassay framework that merges oligonucleotide-based detection with immunoassay methodologies. Notably, they demonstrate with compelling evidence that this approach facilitates a modified ELISA platform capable of simultaneously quantifying multiple target protein expression levels within a single protein mixture sample.

    Reviewed by eLife

    This article has 6 evaluationsAppears in 1 listLatest version Latest activity
  4. Rapid Cell-Free Combinatorial Mutagenesis Workflow Using Small Oligos Suitable for High-Iteration, Active Learning-Guided Protein Engineering

    This article has 5 authors:
    1. Ryan Godin
    2. Sepehr Hejazi
    3. Bret Lange
    4. Basmala Aldamak
    5. Nigel F. Reuel

    Reviewed by Arcadia Science

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  5. Glycogen Engineering Improves the Starvation Resistance of Mesenchymal stem cells and their Therapeutic Efficacy in Pulmonary Fibrosis

    This article has 10 authors:
    1. Yongyue Xu
    2. Mamatali Rahman
    3. Zhaoyan Wang
    4. Bo Zhang
    5. Hanqi Xie
    6. Lei Wang
    7. Haowei Xu
    8. Xiaodan Sun
    9. Shan Cheng
    10. Qiong Wu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable study presents a novel approach to enhance the therapeutic potential of mesenchymal stromal cells (MSCs) by genetically modifying their glycogen synthesis pathway, resulting in increased glycogen accumulation and improved cell survival under starvation conditions, particularly in the context of experimental pulmonary fibrosis. The methods and findings are generally solid and could be strengthened by investigating the kinetics of persistence, the immunomodulatory effects, and the underlying improved mechanism of action of MSCs in this pulmonary fibrosis model. If confirmed, this approach could suggest potential methods to improve the therapeutic functionality of MSCs in cell therapy strategies.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  6. Open-source cell culture automation system with integrated cell counting for passaging microplate cultures

    This article has 10 authors:
    1. Greg Courville
    2. Shivanshi Vaid
    3. Alexis Toruño
    4. Paul Lebel
    5. Joana P. Cabrera
    6. Preethi Raghavan
    7. Axel Jacobsen
    8. George Bell
    9. Manuel D. Leonetti
    10. Rafael Gómez-Sjöberg

    Reviewed by Arcadia Science

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  7. De novo design of miniprotein agonists and antagonists targeting G protein-coupled receptors

    This article has 46 authors:
    1. Edin Muratspahić
    2. David Feldman
    3. David E. Kim
    4. Xiangli Qu
    5. Ana-Maria Bratovianu
    6. Paula Rivera-Sánchez
    7. Federica Dimitri
    8. Jason Cao
    9. Brian P. Cary
    10. Matthew J. Belousoff
    11. Peter Keov
    12. Qingchao Chen
    13. Yue Ren
    14. Justyn Fine
    15. Isaac Sappington
    16. Thomas Schlichthaerle
    17. Jason Z. Zhang
    18. Arvind Pillai
    19. Ljubica Mihaljević
    20. Magnus Bauer
    21. Susana Vázquez Torres
    22. Amir Motmaen
    23. Gyu Rie Lee
    24. Long Tran
    25. Xinru Wang
    26. Inna Goreshnik
    27. Dionne K. Vafeados
    28. Justin E. Svendsen
    29. Parisa Hosseinzadeh
    30. Nicolai Lindegaard
    31. Matthäus Brandt
    32. Yann Waltenspühl
    33. Kristine Deibler
    34. Luke Oostdyk
    35. William Cao
    36. Lakshmi Anantharaman
    37. Lance Stewart
    38. Lauren Halloran
    39. Jamie B. Spangler
    40. Patrick M. Sexton
    41. Bryan L. Roth
    42. Brian E. Krumm
    43. Denise Wootten
    44. Christopher G. Tate
    45. Christoffer Norn
    46. David Baker

    Reviewed by Arcadia Science

    This article has 2 evaluationsAppears in 2 listsLatest version Latest activity
  8. CRISPR-Edited DPSCs, Constitutively Expressing BDNF Enhance Dentin Regeneration in Injured Teeth

    This article has 5 authors:
    1. Ji Hyun Kim
    2. Muhammad Irfan
    3. Sreelekshmi Sreekumar
    4. Atsawasuwan Phimon
    5. Seung Chung
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study on the effect of the trophic factor BDNF upon dental cells is an understudied subject that is relevant to dental regeneration and repair. Given that the topic is new and has not been covered previously, the report is a useful foray into a new area of investigation, although several experimental results could be strengthened. The connection of BDNF and dental health is a solid attempt in potentially translating trophic factor signaling clinically, which has been stymied in past efforts.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  9. Calibration and validation strategy for electromechanical cardiac digital twins

    This article has 7 authors:
    1. Zhinuo Jenny Wang
    2. Maxx Holmes
    3. Ruben Doste
    4. Julia Camps
    5. Francesca Margara
    6. Mariano Vazquez
    7. Blanca Rodriguez
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This is a potentially important study that explores the relevant range of parameter values for calibration and validation of cardiac electromechanics in ventricular models. Although much of the work presented is solid, the evidence provided to support the authors' key scientific claims is incomplete, especially as it relates to the emphasis on standardized validation and verification approaches. Notably, the level of model personalization presented in this work falls short of the threshold for what could reasonably be called a "digital twin", even by the relatively relaxed standards that have emerged in computational physiology and related fields in recent years.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  10. Bacterial Wastewater-Based Epidemiology Using Surface-Enhanced Raman Spectroscopy and Machine Learning

    This article has 7 authors:
    1. Liam K. Herndon
    2. Yirui Zhang
    3. Fareeha Safir
    4. Babatunde Ogunlade
    5. Halleh B. Balch
    6. Alexandria B. Boehm
    7. Jennifer A. Dionne

    Reviewed by Arcadia Science

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
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