Bioengineering

A high-throughput assay for the measurement of Ca ²⁺ -oscillations and insulin release from uniformly sized β-cell spheroids

1. Stijn Robben
2. Patricia Davidson
3. Rita S. Rodrigues Ribeiro
4. Thomas Voets

• Curated by eLife

  eLife Assessment

  In this valuable study, Robben et al. describe a 3D beta-cell spheroid platform, a tool allowing high-throughput monitoring of cytoplasmic calcium concentrations and insulin secretion, with calcium signals comparable to those recorded in primary pancreatic islets. The authors demonstrate the method by culturing MIN6 cells in a 3D culture system, and show solid evidence of its utility by recording calcium signals in a high-throughput format and characterizing these calcium signals using pharmacological tools. This highlights the potential utility of the 3D beta-cell spheroids for screening new pharmacological modulators of pancreatic beta-cell function.

Reviewed by eLife

Autonomous Liquid-handling Robotics Scripting for Accessible and Responsible Protein Engineering

1. Yuan Gao
2. Yizhou Luo
3. Wenzhuo Li
4. Yunquan Lan
5. Han Jiang
6. Yongcan Chen
7. Xiao Yi
8. Boyang Li
9. Hamid Alinejad-Rokny
10. Teng Wang
11. Lihao Fu
12. Min Yang
13. Tong Si

Reviewed by Arcadia Science

Direct MRI of Collagen

1. Jason Daniel van Schoor
2. Markus Weiger
3. Emily Louise Baadsvik
4. Klaas Paul Pruessmann

• Curated by eLife

  eLife Assessment

  This fundamental work substantially advances our understanding of a major research question: whether collagen can be directly imaged with MRI. The evidence supporting the conclusion is compelling, with methods, data, and analyses that are more rigorous than those currently considered state-of-the-art. The work will be of high interest to MR physicists and clinicians, as collagen is the most abundant protein in the human body and plays an essential role in health.

Reviewed by eLife

Targeted computational design of an interleukin-7 superkine with enhanced folding efficiency and immunotherapeutic efficacy

1. See-Khai Lim
2. Wen-Ching Lin
3. Yi-Chung Pan
4. Sin-Wei Huang
5. Yao-An Yu
6. Cheng-Hung Chang
7. Che-Ming Jack Hu
8. Chung-Yuan Mou
9. Kurt Yun Mou

• Curated by eLife

  eLife Assessment

  This important study presents the rational redesign and engineering of interleukin-7. The data from the integrated approach of using computational, biophysical, and cellular experiments are convincing. This paper is broadly relevant to those studying immunomodulation using biologics.

Reviewed by eLife

Generation of Chlorella vulgaris starch mutants and their biomass and lipid productivities under different culture media

1. Ana C. Ramos
2. Anna Hamilton
3. Isabel Molina
4. Patrick J. McGinn
5. Sharon Regan

Reviewed by Arcadia Science

Reversing transgene silencing via targeted chromatin editing

1. Sebastian Palacios
2. Elia Salibi
3. Eric Lu
4. Ron Weiss
5. Thorsten M. Schlaeger
6. James J. Collins
7. Domitilla Del Vecchio

Reviewed by Arcadia Science

Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain

1. Qingqing Feng
2. Keman Cheng
3. Lizhuo Zhang
4. Xiaoyu Gao
5. Jie Liang
6. Guangna Liu
7. Nana Ma
8. Chen Xu
9. Ming Tang
10. Liting Chen
11. Xinwei Wang
12. Xuehui Ma
13. Jiajia Zou
14. Quanwei Shi
15. Pei Du
16. Qihui Wang
17. Guangjun Nie
18. Xiao Zhao

Reviewed by Rapid Reviews Infectious Diseases

Kinetic properties of optogenetic site-specific DNA recombination by LiCre- loxP

1. Alice Dufour
2. Hélène Duplus-Bottin
3. Thomas Boukéké-Lesplulier
4. Eliane Casassa
5. Gérard Triqueneaux
6. Léo Tarbouriech
7. Camille Darthenay-Kiennemann
8. Agnès Dumont
9. Catherine Moali
10. Franck Vittoz
11. Daniel Jost
12. Gaël Yvert

Reviewed by Review Commons

RamanBot: Versatile high throughput Raman system

1. Khaled Atia
2. Robert Hunter
3. Meshach Asare-Werehene
4. Benjamin K. Tsang
5. Hanan Anis

Reviewed by Arcadia Science

Matrix viscoelasticity regulates dendritic cell migration and immune priming

1. Wei-Hung Jung
2. Emie Humann
3. Joshua M Price
4. Yoav Binenbaum
5. Azra Haseki
6. Sanjana Iyer
7. Daivd J Mooney

Reviewed by preLights