RamanBot: Versatile high throughput Raman system

Raman spectroscopy is a powerful tool for qualitative and quantitative analysis in various scientific and industrial fields. However, the development of multisample automated screening remains relatively unexplored. In this paper, we develop RamanBot, a low-cost, easy-to-assemble, and automated Raman spectroscopy system designed for efficient signal collection from samples stored in different types of containers. For the first time, the proposed device introduces the Cartesian motion system, commonly used in 3D printers, to Raman spectroscopy automation. This is achieved by replacing the extrusion head of a commercially available 3D printer with a novel designed “Raman Head”. The Raman head integrates all the necessary optical components required for in-place sample excitation and signal collection. A multimode fiber is used to deliver the excitation laser to the Raman head, whereas the collected Raman signal is delivered to the spectrometer via a fiber bundle. The motion system is programmed to scan predefined sample arrangements using the standard programming language for computer numerical control (G-code). The effect of movement precision on the Raman signal is studied. The introduced device is used in the quantitative analysis of ethanol and methanol. In addition, RamanBot is used to screen six eggs in their commercial packaging with minimal human intervention. The results show that the system is highly stable and capable of delivering reliable Raman measurements, making it a promising solution for high-throughput Raman spectroscopy applications.