Biochemistry

Cryo‐EM structures of multiple‐peptide resistance factor ( MprF ) from Pseudomonas aeruginosa

1. Shaileshanand Jha
2. Kutti R. Vinothkumar

Reviewed by Review Commons

Mediator Subunit MED16 Collaborates with UBP1-TFCP2 to Control Transcriptional Activation or Repression via Promoter Positional Specificity

1. Yuanming Zheng
2. Xiaying Zhao
3. Ming Yang
4. Xinyi Yang
5. Huanzhang Zhu
6. Xiaofei Yu
7. Qiang Zhou
8. Gang Wang

• Curated by eLife

  eLife Assessment

  The characterization of a dissociable Mediator subunit implicated in cellular pathways, particularly lung alveolar function and HIV latency, would be conceptually interesting. The authors have preliminary evidence for a stable Med16 subcomplex that may regulate specific genes. This work is useful in that it points to interactions between Med16 and UBP1, but the evidence is preliminary and incomplete.

Reviewed by eLife

Isolation of functional lysosomes from skeletal muscle

1. Thulasi Mahendran
2. Anastasiya Kuznyetsova
3. Neushaw Moradi
4. David A. Hood

Reviewed by Arcadia Science

The TTLL10 polyglycylase is stimulated by tubulin glutamylation and inhibited by polyglycylation

1. Steven W Cummings
2. Yan Li
3. Jeffrey O Spector
4. Christopher Kim
5. Antonina Roll-Mecak

• Curated by eLife

  eLife Assessment

  In their study, Cummings et al. provide a valuable advance in understanding the hierarchical regulation of tubulin polyglycylation, demonstrating that TTLL8 initiates monoglycylation which is a prerequisite for TTLL10-mediated polyglycylation. The evidence supporting these mechanistic insights is solid, relying on a compelling combination of purified biochemical assays, mass spectrometry, and microscopy. The work is further valued for revealing an unexpected crosstalk between polyglycylation and polyglutamylation that ensures a balanced post-translational modification landscape for proper cilia function.

Reviewed by eLife

RamanMAE: Masked Autoencoders Enable Efficient Molecular Imaging by Learning Biologically Meaningful Spectral Representations

1. Santosh Kumar Paidi
2. Parul Maheshwari

Reviewed by Arcadia Science