Biochemistry

Allosteric modulation by the fatty acid site in the glycosylated SARS-CoV-2 spike

1. A Sofia F Oliveira
2. Fiona L Kearns
3. Mia A Rosenfeld
4. Lorenzo Casalino
5. Lorenzo Tulli
6. Imre Berger
7. Christiane Schaffitzel
8. Andrew D Davidson
9. Rommie E Amaro
10. Adrian J Mulholland

• Curated by eLife

  eLife Assessment

  This manuscript focuses on understanding if and how the glycosylation of SARS-CoV2 spike protein affects a putative allosteric network of interactions controlled by the binding of a fatty acid. The main conclusion is that glycans do not significantly affect the network of allosteric interactions. This valuable information - albeit mainly consisting of negative results - is based on convincing evidence. It will be of interest to scientists focusing on SARS CoV2 protein structure and dynamics.

Reviewed by eLife

AI-based discovery and cryoEM structural elucidation of a KATP channel pharmacochaperone

1. Assmaa Elsheikh
2. Camden M Driggers
3. Ha H Truong
4. Zhongying Yang
5. John Allen
6. Niel M Henriksen
7. Katarzyna Walczewska-Szewc
8. Show-Ling Shyng

• Curated by eLife

  eLife Assessment

  This important study demonstrates that screening by artificial intelligence can identify relevant novel compounds for interacting with KATP channels. The experimental work is compelling. The broader significance of this work relates to the possibility that KATP channel mutations linked to congenital hyperinsulinism may be effectively rescued to the cell surface with a drug, which could normalize insulin secretion or enhance the effectiveness of existing KATP channel activators such as diazoxide.

Reviewed by eLife

Structure of the Calvin-Benson-Bassham sedoheptulose-1,7-bisphosphatase from the model microalga Chlamydomonas reinhardtii

1. Théo Le Moigne
2. Martina Santoni
3. Lucile Jomat
4. Stéphane D Lemaire
5. Mirko Zaffagnini
6. Nicolas Chéron
7. Julien Henri

• Curated by eLife

  eLife Assessment

  This study provides a valuable structural analysis of the Sedoheptulose-1,7-Bisphosphatase (SBPase) from Chlamydomonas reinhardtii. The data presented are solid and based on X-ray structures of the CrSBPase in an oxidized and reduced state, the authors identify a disulfide bond in close proximity to the dimer interface. They show that the redox-state of the CrSBPase impacts its oligomeric state and might also influence the activity of the protein.

Reviewed by eLife

In vivo exchange of glucose and lactate between photoreceptors and the retinal pigment epithelium

1. Daniel T Hass
2. Elizabeth Giering
3. John YS Han
4. Celia M Bisbach
5. Kriti Pandey
6. Brian M Robbings
7. Thomas O Mundinger
8. Nicholas D Nolan
9. Stephen H Tsang
10. Neal S Peachey
11. Nancy J Philp
12. James B Hurley

• Curated by eLife

  eLife Assessment

  This research is valuable as it investigates metabolic shuttling between photoreceptors and retinal pigment epithelium (RPE) using in vivo infusion techniques and mouse models. The authors find that the retina significantly relies on circulating glucose, with photoreceptors being the primary consumers of glucose, which is convincing. However, the study has incomplete evidence to support the claims that photoreceptors can use lactate as a fuel source, that lactate exported from photoreceptors is utilized by the RPE, and that lactate contributes to the TCA cycle in the RPE. These claims need substantial revision to include potential alternative explanations or perform key experiments.

Reviewed by eLife

Unconventional structure and function of PHD domains from Additional Sex Combs-like proteins

1. CJ Reddington
2. AR Walsh
3. C Göbl
4. PD Mace

Reviewed by Review Commons

Butyrolactol A potentiates caspofungin efficacy against resistant fungi via phospholipid flippase inhibition

1. Xuefei Chen
2. H. Diessel Duan
3. Michael J. Hoy
4. Kalinka Koteva
5. Michaela Spitzer
6. Allison K. Guitor
7. Emily Puumala
8. Aline A. Fiebig
9. Guanggan Hu
10. Bonnie Yiu
11. Sommer Chou
12. Zhuyun Bian
13. Yeseul Choi
14. Amelia Bing Ya Guo
15. Wenliang Wang
16. Sheng Sun
17. Nicole Robbins
18. Anna Floyd Averette
19. Michael A. Cook
20. Ray Truant
21. Lesley T. MacNeil
22. Eric D. Brown
23. James W. Kronstad
24. Brian K. Coombes
25. Leah E. Cowen
26. Joseph Heitman
27. Huilin Li
28. Gerard D. Wright

Reviewed by PREreview

Enzymatic protein fusions with 100% product yield

1. Adrian CD Fuchs

• Curated by eLife

  eLife Assessment

  This revision of important work is a versatile addition to the chemical protein modifications and bioconjugation toolbox in synthetic biology. The technology developed cleverly uses Connectase to irreversibly fuse proteins of interest together so they can be studied in their native context, with compelling well-controlled data showing the technique works for various protein partners. This work will help multiple fields to explore multi-function constructs in basic synthetic biology. This work will also be of interest to those studying fusion oncoproteins commonly expressed in various human pathologies.

Reviewed by eLife

Hepatic HKDC1 deletion alleviates western diet-induced MASH in mice

1. Kai Xu
2. Irene Corona-Avila
3. María Dolores Frutos
4. María Ángeles Núñez-Sánchez
5. Dhruvi Makhanasa
6. Pratham Viral Shah
7. Grace Guzman
8. Bruno Ramos-Molina
9. Medha Priyadarshini
10. Md. Wasim Khan

Reviewed by PREreview, Arcadia Science

Protein codes promote selective subcellular compartmentalization

1. Henry R. Kilgore
2. Itamar Chinn
3. Peter G. Mikhael
4. Ilan Mitnikov
5. Catherine Van Dongen
6. Guy Zylberberg
7. Lena Afeyan
8. Salman F. Banani
9. Susana Wilson-Hawken
10. Tong Ihn Lee
11. Regina Barzilay
12. Richard A. Young

Reviewed by Arcadia Science

Allosteric inhibition of trypanosomatid pyruvate kinases by a camelid single-domain antibody

1. Joar Esteban Pinto Torres
2. Mathieu Claes
3. Rik Hendrickx
4. Meng Yuan
5. Natalia Smiejkowska
6. Pieter Van Wielendaele
7. Aysima Hacisuleyman
8. Hans De Winter
9. Serge Muyldermans
10. Paul AM Michels
11. Malcolm D Walkinshaw
12. Wim Versées
13. Guy Caljon
14. Stefan Magez
15. Yann G-J Sterckx

• Curated by eLife

  eLife Assessment

  This work presents valuable data demonstrating that a camelid single-domain antibody can selectively inhibit a key glycolytic enzyme in trypanosomes via an allosteric mechanism. The claim that this information can be exploited for the design of novel chemotherapeutics is solid but limited by the modest effects on parasite growth, as well as the lack of evidence for cellular target engagement in vivo.

Reviewed by eLife