Biochemistry

ATP-independent phosphate recycling on AGC kinase activation loops induced by alkali metal ions

1. Koji Kubouchi
2. Hideyuki Mukai

• Curated by eLife

  eLife Assessment

  AGC kinases, such as PKN1, are regulated by activation loop phosphorylation. This paper reports that exposing cells to high concentrations of monovalent cations induces rapid activation loop dephosphorylation, with rapid re-phosphorylation when physiological salt is restored. Re-phosphorylation is apparently independent of ATP or candidate kinases, and the paper presents an extraordinary and unconventional mechanism involving phosphate exchange between the activation loop and an unknown acceptor molecule. The findings are intriguing and the approach is logical, but the evidence is incomplete and the significance unclear until the biochemical mechanism is identified.

Reviewed by eLife

Viral proteins activate PARIS-mediated tRNA degradation and viral tRNAs rescue infection

1. Nathaniel Burman
2. Svetlana Belukhina
3. Florence Depardieu
4. Royce A. Wilkinson
5. Mikhail Skutel
6. Andrew Santiago-Frangos
7. Ava B. Graham
8. Alexei Livenskyi
9. Anna Chechenina
10. Natalia Morozova
11. Trevor Zahl
12. William S. Henriques
13. Murat Buyukyoruk
14. Christophe Rouillon
15. Lena Shyrokova
16. Tatsuaki Kurata
17. Vasili Hauryliuk
18. Konstantin Severinov
19. Justine Groseille
20. Agnès Thierry
21. Romain Koszul
22. Florian Tesson
23. Aude Bernheim
24. David Bikard
25. Blake Wiedenheft
26. Artem Isaev

Reviewed by Rapid Reviews Infectious Diseases

Inhibition of NF-κB Signaling by the Reactive Glycolytic Metabolite Methylglyoxal

1. Caroline Stanton
2. Woojin Choi
3. R. Luke Wiseman
4. Michael J. Bollong

Reviewed by preLights

The malate–aspartate shuttle supports thermogenic lipid mobilization in brown adipocytes

1. Michaela Veliova
2. Caroline M. Ferreira
3. Katrina P. Montales
4. Francisco Villalobos
5. Alexandra J. Brownstein
6. Rebeca Acín‐Pérez
7. Gabrielly S. Ferreira
8. Anthony E. Jones
9. Linsey Stiles
10. Ajit S. Divakaruni
11. Marc Liesa
12. Orian S. Shirihai
13. Marcus F. Oliveira

Reviewed by Review Commons

NanoBlocks: creating fluorescent biosensors from affinity binders using competitive binding

1. Silke Denis
2. Vincent Van Deuren
3. Desiree I. Frecot
4. Ulrich Rothbauer
5. Shehab Ismail
6. Alison G. Tebo
7. Peter Dedecker

Reviewed by Arcadia Science

Intrinsic specificity of a ‘core’ TIP60 acetyltransferase complex in Drosophila

1. Silke Krause
2. Marco Borsò
3. Marisa Müller
4. Axel Imhof
5. Zivkos Apostolou
6. Peter B. Becker

Reviewed by Review Commons

Computational design of pH-sensitive binders

1. Green Ahn
2. Brian Coventry
3. Ella Haefner
4. Shayan Sadre
5. Jenny Hu
6. Mimosa Van
7. Buwei Huang
8. Isaac Sappington
9. Adam J. Broerman
10. Mauriz A. Lichtenstein
11. Matthias Glögl
12. Inna Goreshnik
13. Dionne Vafeados
14. David Baker

Reviewed by preLights

Structure-based Design of Chimeric Influenza Hemagglutinins to Elicit Cross-group Immunity

1. Karla M Castro
2. Reyhaneh Ayardulabi
3. Sarah Wehrle
4. Hongrui Cui
5. Sandrine Georgeon
6. Joseph Schmidt
7. Shuhao Xiao
8. Nishat Seraj
9. Wayne Harshbarger
10. Corey P Mallett
11. Ventzislav Vassilev
12. Xavier Saelens
13. Bruno E Correia

• Curated by eLife

  eLife Assessment

  This important study presents a thoughtful design and characterization of chimeric influenza hemagglutinin (HA) head domains combining elements of distinct receptor-binding sites. The results provide convincing evidence that polyclonal cross-group responses to influenza A virus can be elicited by a single immunization. While the mechanistic basis of heterotrimer formation and immunodominance differences remains unclear, the authors provide new insights for protein design, vaccinology, and computational vaccine design.

Reviewed by eLife