Biochemistry

Tentative evidence that aging is caused by a small number of interacting processes

1. Axel Kowald
2. Thomas B L Kirkwood

Reviewed by PREreview

MCAK recognizes the nucleotide-dependent feature at growing microtubule ends

1. Wei Chen
2. Yin-Long Song
3. Jian-Feng He
4. Xin Liang

• Curated by eLife

  eLife Assessment

  This work presents valuable new information on the microtubule-binding mode of the microtubule kinesin-13, MCAK, the authors use quantitative single-molecule studies to propose that MCAK preferentially binds to a GDP-Pi-tubulin portion of the microtubule end. However, the evidence provided to support this claim remains incomplete and would benefit from more rigorous methodology particularly the diffraction limited experiments do not provide sufficient spatial resolution to support the authors' conclusions. In addition, a more through discussion of the existing literature would further strengthen the manuscript.

Reviewed by eLife

Coupled equilibria of dimerization and lipid binding modulate SARS Cov 2 Orf9b interactions and interferon response

1. CJ San Felipe
2. Jyoti Batra
3. Monita Muralidharan
4. Shivali Malpotra
5. Durga Anand
6. Rachel Bauer
7. Kliment A Verba
8. Danielle L Swaney
9. Nevan J Krogan
10. Michael Grabe
11. James S Fraser

• Curated by eLife

  eLife Assessment

  This fundamental study demonstrates that lipid binding can regulate the dimerization state of the SARS-CoV2 Orf9b protein. The data from biophysical and cellular experiments along with mathematical modeling are compelling. This paper is broadly relevant to those studying coupled equilibria across all aspects of biology.

Reviewed by eLife

Chronobot: Deep learning guided time-resolved cryo-EM captures molecular choreography of RecA in homology search

1. Märt-Erik Mäeots
2. Simon Tupin
3. Mohammad M. N. Esfahani
4. Juan B. Rodriguez Molina
5. Julie A. Clapperton
6. Aran Amin
7. Albane Imbert
8. Radoslav I. Enchev

Reviewed by PREreview

Crosslinking by ZapD drives the assembly of short FtsZ filaments into toroidal structures in solution

1. Adrián Merino-Salomón
2. Jonathan Schneider
3. Leon Babl
4. Jan-Hagen Krohn
5. Marta Sobrinos-Sanguino
6. Tillman Schäfer
7. Juan Ramon Luque-Ortega
8. Carlos Alfonso
9. Mercedes Jiménez
10. Marion Jasnin
11. Petra Schwille
12. Germán Rivas

• Curated by eLife

  eLife Assessment

  The formation of the Z-ring at the time of bacterial cell division interests researchers working towards understanding cell division across all domains of life. The manuscript by Jasnin et al reports the cryoET structure of toroid assembly formation of FtsZ filaments driven by ZapD as the cross linker. The findings are important and have the potential to open a new dimension in the field, and the evidence to support these exciting claims is solid.

Reviewed by eLife

A Miniaturized, High-Throughput Aqueous Solvent-Centric Method for Protein Solubility Screening

1. Adrian Svoboda
2. Marina Molineris
3. Theodora Tureckiova
4. Klára Hlouchová
5. Tomáš Pluskal
6. Teo Hebra

Reviewed by Arcadia Science

Allosteric modulation of dimeric GPR3 by ligands in the dimerization interface

1. Zeming Qiu
2. Wei Wang
3. Yingying Nie
4. Junxiang Lin
5. Beimeng Zhang
6. Haonan Xing
7. Sanduo Zheng

• Curated by eLife

  eLife Assessment

  Qiu et al. present multiple dimeric structures of GPR3, which reveal the binding mode of the inverse agonist AF64394. The findings provide important insights into the regulation of GPCR3 and potentially other related orphan GPCRs. The authors present convincing evidence of their claims through thoughtful analysis of their cryo-EM structures, mutagenesis, and cell-based assays. This work will be of interest to GPCR investigators, especially those studying the signaling of orphan receptors.

Reviewed by eLife

Inherited CDA-I disease: anemia-associated mutations disrupt CDIN1-Codanin1 complex

1. Martin Stojaspal
2. Tomáš Brom
3. Ivona Nečasová
4. Tomáš Janovič
5. Pavel Veverka
6. Naina Verma
7. Lukáš Uhrík
8. Lenka Hernychova
9. Ctirad Hofr

Reviewed by Review Commons

Amyloid-β fibrils accumulated in preeclamptic placentas suppress cytotrophoblast syncytialization

1. Kaho Nishioka
2. Midori Ikezaki
3. Naoyuki Iwahashi
4. Miyu Arakawa
5. Momo Fukushima
6. Noa Mori
7. Mika Mizoguchi
8. Yuko Horiuchi-Tanizaki
9. Megumi Fujino
10. Takami Tomiyama
11. Yoshito Ihara
12. Kenji Uchimura
13. Kazuhiko Ino
14. Kazuchika Nishitsuji

Reviewed by Review Commons

Occupancy of the HbYX hydrophobic pocket is sufficient to induce gate opening in the archaeal 20S proteasomes

1. Janelle JY Chuah
2. Madalena R Daugherty
3. David M Smith

• Curated by eLife

  eLife Assessment

  This valuable manuscript describes cryo-EM structures of archaeal proteasomes that reveal insights into how occupancy of binding pockets on the 20S proteasome regulates proteasome gating. The evidence supporting these claims is convincing, although the extrapolation of these findings to the more complex eukaryotic proteasome may prove challenging. This work will be of high interest to researchers interested in proteasome structure and regulation.

Reviewed by eLife