Biochemistry

A miniaturized, high-throughput aqueous solvent-centric method for protein solubility screening

1. Adrian Svoboda
2. Marina Molineris
3. Theodora Tureckiova
4. Klára Hlouchová
5. Tomáš Pluskal
6. Teo Hebra

Reviewed by Arcadia Science

Allosteric modulation of dimeric GPR3 by ligands in the dimerization interface

1. Zeming Qiu
2. Wei Wang
3. Yingying Nie
4. Junxiang Lin
5. Beimeng Zhang
6. Haonan Xing
7. Sanduo Zheng

• Curated by eLife

  eLife Assessment

  Qiu et al. present multiple dimeric structures of GPR3, which reveal the binding mode of the inverse agonist AF64394. The findings provide important insights into the regulation of GPCR3 and potentially other related orphan GPCRs. The authors present convincing evidence of their claims through thoughtful analysis of their cryo-EM structures, mutagenesis, and cell-based assays. This work will be of interest to GPCR investigators, especially those studying the signaling of orphan receptors.

Reviewed by eLife

Inherited CDA-I disease: anemia-associated mutations disrupt CDIN1-Codanin1 complex

1. Martin Stojaspal
2. Tomáš Brom
3. Ivona Nečasová
4. Tomáš Janovič
5. Pavel Veverka
6. Naina Verma
7. Lukáš Uhrík
8. Lenka Hernychova
9. Ctirad Hofr

Reviewed by Review Commons

Amyloid-β fibrils accumulated in preeclamptic placentas suppress syncytialization of cytotrophoblasts

1. Kaho Nishioka
2. Midori Ikezaki
3. Naoyuki Iwahashi
4. Miyu Arakawa
5. Momo Fukushima
6. Noa Mori
7. Mika Mizoguchi
8. Yuko Horiuchi-Tanizaki
9. Megumi Fujino
10. Takami Tomiyama
11. Yoshito Ihara
12. Kenji Uchimura
13. Kazuhiko Ino
14. Kazuchika Nishitsuji

Reviewed by Review Commons

Occupancy of the HbYX hydrophobic pocket is sufficient to induce gate opening in the archaeal 20S proteasomes

1. Janelle JY Chuah
2. Madalena R Daugherty
3. David M Smith

• Curated by eLife

  eLife Assessment

  This valuable manuscript describes cryo-EM structures of archaeal proteasomes that reveal insights into how occupancy of binding pockets on the 20S proteasome regulates proteasome gating. The evidence supporting these claims is convincing, although the extrapolation of these findings to the more complex eukaryotic proteasome may prove challenging. This work will be of high interest to researchers interested in proteasome structure and regulation.

Reviewed by eLife

Rac1 Selectively Binds a Specific Lamellipodin Isoform via a Noncanonical Helical Interface

1. Tong Gao
2. Pingfeng Zhang
3. Alison M. Kurimchak
4. James S. Duncan
5. Jinhua Wu

Reviewed by Arcadia Science

The temperature dependence of binding entropy is a selective pressure in protein evolution

1. Rosemary Georgelin
2. Hannah Bott
3. Joe A. Kaczmarski
4. Rebecca Frkic
5. Li Lynn Tan
6. Nobuhiko Tokuriki
7. Matthew A. Spence
8. Colin J. Jackson

Reviewed by PREreview

Categorizing prediction modes within low-pLDDT regions of AlphaFold2 structures

1. Christopher J Williams
2. Vincent B Chen
3. David C Richardson
4. Jane S Richardson

Reviewed by Arcadia Science

Phage-displayed synthetic library and screening platform for nanobody discovery

1. Baolong Xia
2. Ah-Ram Kim
3. Feimei Liu
4. Myeonghoon Han
5. Emily Stoneburner
6. Stephanie Makdissi
7. Francesca Di Cara
8. Stephanie E Mohr
9. Aaron Ring
10. Norbert Perrimon

• Curated by eLife

  eLife Assessment

  This important study presents an alternative platform for nanobody discovery using phage-displayed synthetic libraries. The evidence supporting the platform, which is used to isolate and validate nanobodies targeting Drosophila secreted proteins, is compelling. By making the library openly accessible, this provides an excellent resource to the wider scientific community. The paper presents a detailed protocol for nanobody screening; as this protocol is refined and optimized over time, this will increase the success rate for discovering nanobodies with improved properties using this alternative platform.

Reviewed by eLife

Cardiolipin deficiency disrupts electron transport chain to drive steatohepatitis

1. Marisa J Brothwell
2. Guoshen Cao
3. J Alan Maschek
4. Annelise M Poss
5. Alek D Peterlin
6. Liping Wang
7. Talia B Baker
8. Justin L Shahtout
9. Piyarat Siripoksup
10. Quentinn J Pearce
11. Jordan M Johnson
12. Fabian M Finger
13. Alexandre Prola
14. Sarah A Pellizzari
15. Gillian L Hale
16. Allison M Manuel
17. Shinya Watanabe
18. Edwin R Miranda
19. Kajsa E Affolter
20. Trevor S Tippetts
21. Linda S Nikolova
22. Ran Hee Choi
23. Stephen T Decker
24. Mallikarjun Patil
25. J Leon Catrow
26. William L Holland
27. Sara M Nowinski
28. Daniel S Lark
29. Kelsey H Fisher-Wellman
30. Patrice N Mimche
31. Kimberley J Evason
32. James E Cox
33. Scott A Summers
34. Zach Gerhart-Hines
35. Katsuhiko Funai

• Curated by eLife

  eLife Assessment

  This paper reports on a correlation between diminished cardiolipin content and the severity of steatohepatitis in human subjects. This is supported further by experimental evidence from mice in which the gene encoding a key enzyme in cardiolipin synthesis has been compromised in the liver. The correlations established between lipidology, mitochondrial function, and the induction of respiration and oxidative stress are notable and will be useful to researchers in the field. However, given that the causal relationship between lipid perturbation and the progression of steatohepatitis implied in the title has not been tested experimentally, the evidence supporting the paper's key conclusion is incomplete.

Reviewed by eLife