Biochemistry

A high-resolution analysis of arrestin2 interactions responsible for CCR5 endocytosis

1. Ivana Petrovic
2. Samit Desai
3. Polina Isaikina
4. Layara Akemi Abiko
5. Anne Spang
6. Stephan Grzesiek

• Curated by eLife

  eLife Assessment

  The authors investigate arrestin2-mediated CCR5 endocytosis in the context of clathrin and AP2 contributions. Using an extensive set of NMR experiments, and supported by microscopy and other biophysical assays, the authors provide compelling data on the roles of AP2 and clathrin in CCR5 endocytosis. This important work will appeal to an audience beyond those studying chemokine receptors, including those studying GPCR regulation and trafficking. The distinct role of AP2 and not clathrin will be of particular interest to those studying GPCR internalization mechanisms.

Reviewed by eLife

Mitochondrial fatty acid oxidation is stimulated by red light irradiation

1. Manuel Alejandro Herrera
2. Camille C. Caldeira da Silva
3. Maiza Von Dentz
4. Mauricio S. Baptista
5. Alicia J. Kowaltowski

Reviewed by preLights

Identification of the regulatory elements and protein substrates of lysine acetoacetylation

1. Qianyun Fu
2. Terry Nguyen
3. Bhoj Kumar
4. Parastoo Azadi
5. Y George Zheng

• Curated by eLife

  eLife Assessment

  This useful study reports a method to detect and analyze a novel post-translational modification, lysine acetoacetylation (Kacac), finding it regulates protein metabolism pathways. The study unveils epigenetic modifiers involved in placing this mark, including key histone acetyltransferases such as p300, and concomitant HDACs, which remove the mark. Proteomic and bioinformatics analysis identified many human proteins with Kacac sites, potentially suggesting broad effects on cellular processes and disease mechanisms. The data presented are solid and the study will be of interest to those studying protein and metabolic regulation.

Reviewed by eLife

Vitamin B12 Supports Skeletal Muscle Oxidative Phosphorylation Capacity in Male Mice

1. Luisa F Castillo
2. Katarina E Heyden
3. Abigail R Williamson
4. Wenxia Ma
5. Olga V Malysheva
6. Nathaniel M Vacanti
7. Anna E Thalacker-Mercer
8. Martha S Field

Reviewed by preLights

Engineered Transdermal Peptide-Recombinant Type III Collagen Hydrogel with Biological Efficacy in Cell Proliferation and Wound Healing

1. Wansen Tan
2. Yue Liu
3. Jingjun Hong

Reviewed by PREreview

RNF13 is a novel interactor of iduronate 2-sulfatase that modifies its glycosylation and maturation

1. Valérie C. Cabana
2. Antoine Y. Bouchard
3. Audrey M. Sénécal
4. Laurent Cappadocia
5. Marc P. Lussier

Reviewed by Arcadia Science

Aspartate transaminases are required for blood development

1. Narges Pourmandi
2. Greggory Myers
3. Arjun Jha
4. Kelsey Temprine
5. Amanda Sankar
6. Nupur K. Das
7. Ridwana Khan
8. Siva Kumar Natarajan
9. Cristina Castillo
10. Peter Sajjakulnukit
11. Noah S. Nelson
12. Matthew D. Perricone
13. Indrani Talukder
14. Aaron D. denDekker
15. Lin Lin
16. Dominik Awad
17. Wesley Huang
18. Lei Yu
19. Navdeep S. Chandel
20. Rami Khoriaty
21. Yatrik M. Shah
22. Costas A. Lyssiotis

Reviewed by preLights

Gut microbe-derived trimethylamine shapes circadian rhythms through the host receptor TAAR5

1. Kala K Mahen
2. William J Massey
3. Danny Orabi
4. Amanda L Brown
5. Thomas C Jaramillo
6. Amy Burrows
7. Anthony J Horak
8. Sumita Dutta
9. Marko Mrdjen
10. Nour Mouannes
11. Venkateshwari Varadharajan
12. Lucas J Osborn
13. Xiayan Ye
14. Dante M Yarbrough
15. Treg Grubb
16. Natalie Zajczenko
17. Rachel Hohe
18. Rakhee Banerjee
19. Pranavi Linga
20. Dev Laungani
21. Adeline M Hajjar
22. Naseer Sangwan
23. Mohammed Dwidar
24. Jennifer A Buffa
25. Garth R Swanson
26. Zeneng Wang
27. Jonathan Mark Brown

• Curated by eLife

  eLife Assessment

  This study presents an important finding linking the bacterial metabolite trimethylamine and its receptor to circadian rhythms and olfaction. The current evidence supporting the claims of the authors is compelling. This work will be of broad interest to researchers interested in nutrition, microbial metabolism, circadian rhythms, and host-microbiome interactions.

Reviewed by eLife

ATP-independent phosphate recycling on AGC kinase activation loops induced by alkali metal ions

1. Koji Kubouchi
2. Hideyuki Mukai

• Curated by eLife

  eLife Assessment

  AGC kinases, such as PKN1, are regulated by activation loop phosphorylation. This paper reports that exposing cells to high concentrations of monovalent cations induces rapid activation loop dephosphorylation, with rapid re-phosphorylation when physiological salt is restored. Re-phosphorylation is apparently independent of ATP or candidate kinases, and the paper presents an extraordinary and unconventional mechanism involving phosphate exchange between the activation loop and an unknown acceptor molecule. The findings are intriguing and the approach is logical, but the evidence is incomplete and the significance unclear until the biochemical mechanism is identified.

Reviewed by eLife