Biochemistry

Acetylation of RORβ by histone acetyltransferase p300 and deacetylation by SIRT1 modulates receptor stability, turnover and transcriptional activity

1. Timothy O’Leary
2. Denis Shutin
3. Dean P. Edwards
4. Patrick R. Griffin
5. Mi Ra Chang

Reviewed by Arcadia Science

Computational design of highly active de novo enzymes

1. Markus Braun
2. Adrian Tripp
3. Morakot Chakatok
4. Sigrid Kaltenbrunner
5. Massimo G. Totaro
6. David Stoll
7. Aleksandar Bijelic
8. Wael Elaily
9. Shlomo Yakir Yakir Hoch
10. Matteo Aleotti
11. Melanie Hall
12. Gustav Oberdorfer

Reviewed by PREreview

Allosteric modulation of the Lon protease by effector binding and local charges

1. Justyne L Ogdahl
2. Peter Chien

Reviewed by PREreview

Mechanistic Studies of Small Molecule Ligands Selective to RNA Single G Bulges

1. Shalakha Hegde
2. Sana Akhter
3. Zhichao Tang
4. Chang Qi
5. Chenguang Yu
6. Anna Lewicka
7. Yu Liu
8. Kushal Koirala
9. Mikhail Reibarkh
10. Kevin P. Battaile
11. Anne Cooper
12. Scott Lovell
13. Erik D. Holmstrom
14. Xiao Wang
15. Joseph A. Piccirilli
16. Qi Gao
17. Yinglong Miao
18. Jingxin Wang

Reviewed by PREreview

The co-receptor Tetraspanin12 directly captures Norrin to promote ligand-specific β-catenin signaling

1. Elise S Bruguera
2. Jacob P Mahoney
3. William I Weis

• Curated by eLife

  eLife Assessment

  This is a fundamental study that addresses the key question of how the tetraspanin Tspan12 functions biochemically as a co-receptor for Norrin to initiate β-catenin signaling. The strength of the work lies in the rigorous and compelling binding analyses involving various purified receptors, co-receptors, and ligands, as well as molecular modeling by AlphaFold that was subsequently validated by an extensive series of mutagenesis experiments. The study advances the field by providing a novel mechanism of co-receptor function and shedding new light on how signaling specificity is achieved in the complex Wnt/Norrin signaling system.

Reviewed by eLife

Structure of the human CTF18 clamp loader bound to PCNA

1. Giuseppina R Briola
2. Muhammad Tehseen
3. Amani Al-Amodi
4. Phong Quoc Nguyen
5. Christos G Savva
6. Samir M Hamdan
7. Alfredo De Biasio

• Curated by eLife

  eLife Assessment

  Briola and co-authors determined the structure of the human CTF18 clamp loader bound to PCNA to high resolution, analyzed the structure, and tested a new mechanism involving a human-specific Ctf18 beta-hairpin docking onto Rfc5, which represents a valuable contribution. The data are solid and complement data recently published by others.

Reviewed by eLife

Protonation/deprotonation-driven switch for the redox stability of low-potential [4Fe-4S] ferredoxin

1. Kei Wada
2. Kenji Kobayashi
3. Iori Era
4. Yusuke Isobe
5. Taigo Kamimura
6. Masaki Marukawa
7. Takayuki Nagae
8. Kazuki Honjo
9. Noriko Kaseda
10. Yumiko Motoyama
11. Kengo Inoue
12. Masakazu Sugishima
13. Katsuhiro Kusaka
14. Naomine Yano
15. Keiichi Fukuyama
16. Masaki Mishima
17. Yasutaka Kitagawa
18. Masaki Unno

• Curated by eLife

  eLife Assessment

  Ferredoxins are ubiquitous electron transfer proteins that drive essential metabolic processes across all domains of life. This fundamental contribution to the field provides the first description of how specific amino acids, though a series of hydrogen bonds, control the ability of iron-sulfur clusters in ferrodoxins to accept and donate electrons. The evidence supporting the conclusions is compelling as is the combined use of neutron crystallography with X-ray crystallography and classical spectral/redox studies.

Reviewed by eLife

Interactions with multiple inner kinetochore proteins determine mitotic localization of FACT

1. Julia Schweighofer
2. Bhagyashree Mulay
3. Ingrid Hoffmann
4. Doro Vogt
5. Marion E. Pesenti
6. Andrea Musacchio

Reviewed by Review Commons

Conformational dynamics and asymmetry in multimodal inhibition of membrane-bound pyrophosphatases

1. Jianing Liu
2. Anokhi Shah
3. Xinyu Liu
4. Joshua L. Wort
5. Yue Ma
6. Katie Hardman
7. Niklas G. Johansson
8. Orquidea Ribeiro
9. Adam Brookfield
10. Alice Bowen
11. Jari Yli-Kauhaluoma
12. Henri Xhaard
13. Lars J.C. Jeuken
14. Adrian Goldman
15. Christos Pliotas
16. Keni Vidilaseris

• Curated by eLife

  eLife Assessment

  The current study presents useful findings about the inhibition of a membrane pyrophosphatase by non-hydrolyzable phosphonate substrate analogs. The study proposes a model in which the two monomers in a functional dimer interact with the phosphonate molecules in an asymmetric fashion. While asymmetry has been previously demonstrated through other studies, the DEER spectroscopy data presented in the current study provide incomplete evidence of the proposed asymmetry near the binding site.

Reviewed by eLife

Daptomycin forms a stable complex with phosphatidylglycerol for selective uptake to bacterial membrane

1. Pragyansree Machhua
2. Vignesh Gopalakrishnan Unnithan
3. Yu Liu
4. Yiping Jiang
5. Lingfeng Zhang
6. Zhihong Guo

• Curated by eLife

  eLife Assessment

  This valuable study describes the molecular mechanism of daptomycin insertion into bacterial membranes. The authors provide solid in vitro evidence for the early events of daptomycin interaction with phospholipid headgroups and stronger, specific interaction with phosphatidylglycerol. This work will be of interest to bacterial membrane biologists and biochemists working in the antimicrobial resistance field.

Reviewed by eLife