Biochemistry

SARS-CoV-2 methyltransferase nsp10-16 in complex with natural and drug-like purine analogs for guiding structure-based drug discovery

1. Viviane Kremling
2. Sven Falke
3. Yaiza Fernández-García
4. Christiane Ehrt
5. Antonia Kiene
6. Bjarne Klopprogge
7. Emilie Scheer
8. Fabian Barthels
9. Philipp Middendorf
10. Sebastian Kühn
11. Stephan Günther
12. Matthias Rarey
13. Henry N Chapman
14. Dominik Oberthür
15. Janina Sprenger

• Curated by eLife

  eLife assessment

  This important study describes the crystallographic screening of a number of small molecules against a viral enzyme critical for the 5' capping of SARS-CoV-2 RNA and viral replication. While the high-quality crystal structures and complementary biophysical assays in this study provide solid evidence to support the major claims regarding how these small molecule compounds bind to the viral enzyme, the mismatch between the antiviral activity and binding to the viral enzyme of several small molecule compounds could have been more thoroughly investigated or discussed. This paper would be of interest to the fields of coronavirus biology, structural biology, and drug discovery.

Reviewed by eLife

The LC3-interacting region of NBR1 is a protein interaction hub enabling optimal flux

1. Brian J North
2. Amelia E Ohnstad
3. Michael J Ragusa
4. Christopher J Shoemaker

Reviewed by Review Commons

Proteome of human glioblastoma and meningioma tissue small extracellular vesicles

1. Huaqi Su
2. Adityas Purnianto
3. Andrew Kaye
4. Andrew Morokoff
5. Katherine j Drummond
6. Stanley Stylli
7. Laura J Vella

Reviewed by Arcadia Science

Structural Characterization of DNA Binding Domain of Essential Mammalian Protein TTF 1

1. Gajender Singh
2. Abhinetra Jagdish Bhopale
3. Saloni Khatri
4. Prashant Prakash
5. Rajnish Kumar
6. Sukh Mahendra Singh
7. Samarendra Kumar Singh

Reviewed by Arcadia Science

Effect of truffle extracts on growth of Chlorella vulgaris

1. Anfisa A. Vlasova
2. Olga E. Lipatova
3. Alexander Y. Belyshenko
4. Elena I. Martynova
5. Natalia A. Imidoeva
6. Tamara Y. Telnova
7. Ekaterina V. Malygina
8. Maria M. Morgunova
9. Maria E. Dmitrieva
10. Victoria N. Shelkovnikova
11. Sophia S. Shashkina
12. Tatiana N. Vavilina
13. Denis V. Axenov-Gribanov

Reviewed by Arcadia Science

Specific proteolysis mediated by a p97-directed proteolysis-targeting chimera (PROTAC)

1. Constanza Salinas-Rebolledo
2. Javier Blesa
3. Guillermo Valenzuela-Nieto
4. David Schwefel
5. Natalia López González del Rey
6. Maxs Méndez-Ruette
7. Janine Burkhalter
8. Luis Federico Bátiz
9. Ronald Jara
10. José A. Obeso
11. Pedro Chana-Cuevas
12. Gopal P. Sapkota
13. Alejandro Rojas-Fernandez

Reviewed by Review Commons

Plasticity of the proteasome-targeting signal Fat10 enhances substrate degradation

1. Hitendra Negi
2. Aravind Ravichandran
3. Pritha Dasgupta
4. Shridivya Reddy
5. Ranabir Das

• Curated by eLife

  eLife assessment

  This manuscript probes the ways in which a protein tag might influence the structure, dynamics and stability of a covalently-attached substrate protein. Such findings are of important significance to several fields, particularly in understanding how these influences control the abundance of proteins within a cell. The evidence provided to support the authors' conclusions are, however, incomplete and further control experiments are necessary to fully support the proposed model.

Reviewed by eLife

The TTLL10 polyglycylase is stimulated by tubulin glutamylation and inhibited by polyglycylation

1. Steven W Cummings
2. Yan Li
3. Jeffrey O Spector
4. Christopher Kim
5. Antonina Roll-Mecak

• Curated by eLife

  eLife assessment

  In their manuscript, Cummings et al. use in vitro reconstitution to examine the differential activities of tubulin polyglycylases, providing valuable insights into the enzymatic regulation of microtubule glycylation and its mechanistic role in maintaining cilia function and microtubule dynamics. The convincing evidence, supported by well-designed experiments and appropriate controls, significantly advances our understanding of the tubulin code and its biochemical mechanisms.

Reviewed by eLife

Senolytics enhance longevity in Caenorhabditis elegans by altering betaine metabolism

1. Wenning Lan
2. Xiaolian Xiao
3. Xiaojing Zhang
4. Jingjing Nian
5. Ziran Wang
6. Yajiao Wu
7. Dongcheng Zhang
8. Junkun Chen
9. Wenqiang Bao
10. Chutao Li
11. An Zhu
12. Yun Zhang
13. Fangrong Zhang

Reviewed by PREreview

Dock-and-lock binding of SxIP ligands is required for stable and selective EB1 interactions

1. Teresa Almeida
2. Eleanor Hargreaves
3. Tobias Zech
4. Igor Barsukov

• Curated by eLife

  eLife assessment

  This study provides valuable insights into how the EBH domain of EB1 interacts with SxIP peptides derived from MACF. A convincing description of the thermodynamic and kinetic modes of peptide binding is provided via a combination of solution NMR techniques and ITC. Although consistent with the data, the proposed "dock-and lock" model was not found to be directly supported by evidence. This work will be of interest to structural biologists and biophysicists interested in microtubule cytoskeleton.

Reviewed by eLife