Biochemistry

The method utilized to purify the SARS-CoV-2 N protein can affect its molecular properties

1. Aneta Tarczewska
2. Marta Kolonko-Adamska
3. Mirosław Zarębski
4. Jurek Dobrucki
5. Andrzej Ożyhar
6. Beata Greb-Markiewicz

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In-solution buffer-free digestion for the analysis of SARS-CoV-2 RBD proteins allows a full sequence coverage and detection of post-translational modifications in a single ESI-MS spectrum

1. Luis Ariel Espinosa
2. Yassel Ramos
3. Ivan Andújar
4. Enso Onill Torres
5. Gleysin Cabrera
6. Alejandro Martín
7. Diamilé González
8. Glay Chinea
9. Mónica Becquet
10. Isabel González
11. Camila Canaán-Haden
12. Elías Nelson
13. Gertrudis Rojas
14. Beatriz Pérez-Massón
15. Dayana Pérez-Martínez
16. Tamy Boggiano
17. Julio Palacio
18. Sum Lai Lozada-Chang
19. Lourdes Hernández
20. Kathya Rashida de la Luz Hernández
21. Saloheimo Markku
22. Vitikainen Marika
23. Yury Valdés-Balbín
24. Darielys Santana-Medero
25. Daniel G. Rivera
26. Vicente Vérez-Bencomo
27. Mark Emalfarb
28. Ronen Tchelet
29. Gerardo Guillén
30. Miladys Limonta
31. Eulogio Pimentel
32. Marta Ayala
33. Vladimir Besada
34. Luis Javier González

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Permeant fluorescent probes visualize the activation of SARM1 and uncover an anti-neurodegenerative drug candidate

1. Wan Hua Li
2. Ke Huang
3. Yang Cai
4. Qian Wen Wang
5. Wen Jie Zhu
6. Yun Nan Hou
7. Sujing Wang
8. Sheng Cao
9. Zhi Ying Zhao
10. Xu Jie Xie
11. Yang Du
12. Chi-Sing Lee
13. Hon Cheung Lee
14. Hongmin Zhang
15. Yong Juan Zhao

• Curated by eLife

  Evaluation Summary:

  SARM1, an enzyme that can convert NAD+ to ADP-ribose or cyclic ADP-ribose, is implicated in axon degeneration. This manuscript describes the development of small molecule probes that can detect the activity of SARM1 in live cells. In the course of the work, a small molecule derived from an hypertension drug was discovered as an effective SARM1 inhibitor. Although the activity probes are novel, the mechanism of SARM1 inactivation by dHNN has not been established. The probe and the inhibitor described in the manuscript could lead to future therapeutic development targeting SARM1 to treat axon degeneration.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite

1. Gabriele Cerutti
2. Yicheng Guo
3. Tongqing Zhou
4. Jason Gorman
5. Myungjin Lee
6. Micah Rapp
7. Eswar R. Reddem
8. Jian Yu
9. Fabiana Bahna
10. Jude Bimela
11. Yaoxing Huang
12. Phinikoula S. Katsamba
13. Lihong Liu
14. Manoj S. Nair
15. Reda Rawi
16. Adam S. Olia
17. Pengfei Wang
18. Baoshan Zhang
19. Gwo-Yu Chuang
20. David D. Ho
21. Zizhang Sheng
22. Peter D. Kwong
23. Lawrence Shapiro

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Development of a Simple In Vitro Assay To Identify and Evaluate Nucleotide Analogs against SARS-CoV-2 RNA-Dependent RNA Polymerase

1. Gaofei Lu
2. Xi Zhang
3. Weinan Zheng
4. Jialei Sun
5. Lan Hua
6. Lan Xu
7. Xin-jie Chu
8. Sheng Ding
9. Wen Xiong

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Ebselen derivatives are very potent dual inhibitors of SARS-CoV-2 proteases - PL ^pro and M ^pro in in vitro studies

1. Mikolaj Zmudzinski
2. Wioletta Rut
3. Kamila Olech
4. Jarosław Granda
5. Mirosław Giurg
6. Małgorzata Burda-Grabowska
7. Linlin Zhang
8. Xinyuanyuan Sun
9. Zongyang Lv
10. Digant Nayak
11. Malgorzata Kesik-Brodacka
12. Shaun K. Olsen
13. Rolf Hilgenfeld
14. Marcin Drag

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Identification of low micromolar SARS-CoV-2 M ^pro inhibitors from hits identified by in silico screens

1. Giacomo G. Rossetti
2. Marianna Ossorio
3. Samia Barriot
4. Laurence Tropia
5. Vasilis S. Dionellis
6. Christoph Gorgulla
7. Haribabu Arthanari
8. Peter Mohr
9. Remo Gamboni
10. Thanos D. Halazonetis

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A cysteine protease inhibitor blocks SARS-CoV-2 infection of human and monkey cells

1. Drake M. Mellott
2. Chien-Te Tseng
3. Aleksandra Drelich
4. Pavla Fajtová
5. Bala C. Chenna
6. Demetrios H. Kostomiris
7. Jason Hsu
8. Jiyun Zhu
9. Zane W. Taylor
10. Vivian Tat
11. Ardala Katzfuss
12. Linfeng Li
13. Miriam A. Giardini
14. Danielle Skinner
15. Ken Hirata
16. Sungjun Beck
17. Aaron F. Carlin
18. Alex E. Clark
19. Laura Beretta
20. Daniel Maneval
21. Felix Frueh
22. Brett L. Hurst
23. Hong Wang
24. Klaudia I. Kocurek
25. Frank M. Raushel
26. Anthony J. O’Donoghue
27. Jair Lage de Siqueira-Neto
28. Thomas D. Meek
29. James H. McKerrow

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Cold sensitivity of the SARS-CoV-2 spike ectodomain

1. Robert J. Edwards
2. Katayoun Mansouri
3. Victoria Stalls
4. Kartik Manne
5. Brian Watts
6. Rob Parks
7. Katarzyna Janowska
8. Sophie M. C. Gobeil
9. Megan Kopp
10. Dapeng Li
11. Xiaozhi Lu
12. Zekun Mu
13. Margaret Deyton
14. Thomas H. Oguin
15. Jordan Sprenz
16. Wilton Williams
17. Kevin O. Saunders
18. David Montefiori
19. Gregory D. Sempowski
20. Rory Henderson
21. S. Munir Alam
22. Barton F. Haynes
23. Priyamvada Acharya

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Cytoplasmic short linear motifs in ACE2 and integrin β ₃ link SARS-CoV-2 host cell receptors to mediators of endocytosis and autophagy

1. Johanna Kliche
2. Hanna Kuss
3. Muhammad Ali
4. Ylva Ivarsson

Reviewed by ScreenIT