Colloidal Aggregators in Biochemical SARS-CoV-2 Repurposing Screens
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SciScore for 10.1101/2021.08.31.458413: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Certain caveats should be mentioned. We do not pretend to have undertaken a comprehensive study of the increasingly large literature around drug repurposing for Covid-19. The molecules tested here represent only a subset of those investigated, drawn from an …
SciScore for 10.1101/2021.08.31.458413: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Certain caveats should be mentioned. We do not pretend to have undertaken a comprehensive study of the increasingly large literature around drug repurposing for Covid-19. The molecules tested here represent only a subset of those investigated, drawn from an analysis of some of the literature then available. Also, we have not demonstrated that aggregation is actually occurring in the ACE-2 assay itself, though the lack of inhibition of 3CL-Pro in the presence of detergent fortifies our conclusions for the 12 molecules that inhibited this enzyme. Finally, it is important to note that just because some repurposed drugs aggregate at micromolar concentrations, the repurposing enterprise is not sunk. There are, after all, examples of drugs successfully repurposed, even for Covid-19, and some have even begun from screening hits (though typically they are subsequently modified chemically 44). These caveats should not obscure the main observations from this study. Many drugs repurposed for Covid-19 in biochemical assays are aggregators—still others may be inhibiting through other artifactual mechanisms—and their promise as leads for treating the disease merits reconsideration. More broadly, drugs in repurposing libraries, though de-risked for whole body toxicity, pharmacokinetic exposure and metabolism, are not de-risked for artifactual activity at screening relevant concentrations. More encouragingly, what this study illuminates is a series of rapid assays that can rapidly distinguis...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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