Biochemistry

Prolific Induction of IL-6 in Human Cells by SARS-CoV-2-derived Peptide is Attenuated by Recombinant Human Anti-inflammatory Cytokines made in planta

1. Pieter H. Anborgh
2. Igor Kolotilin
3. Nisha Owens
4. Abdulla Azzam Mahboob

Reviewed by ScreenIT

Proton transfer pathway in anion channelrhodopsin-1

1. Masaki Tsujimura
2. Keiichi Kojima
3. Shiho Kawanishi
4. Yuki Sudo
5. Hiroshi Ishikita

• Curated by eLife

  Evaluation Summary:

  The authors report continuum electrostatics, molecular dynamics and QM/MM simulations to probe the protonation pattern of key residues in anion channelrhodopsin (GtACR1). The findings provide new mechanistic insights into the gating mechanism of GtACR1, and the study will be of potential interest for the community focused on biophysical chemistry, protein simulations and optogenetics. More generally, the study helps highlight that due to potential compensatory effects, care needs to be exercised when interpreting absorption spectra of mutant proteins.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Ultrafast, one-step, and microwave heating-based synthesis of DNA/RNA-AuNP conjugates

1. Mengqi Huang
2. Erhu Xiong
3. Menglu Hu
4. Huahua Yue
5. Tian Tian
6. Debin Zhu
7. Xiaoming Zhou

Reviewed by ScreenIT

pH-dependent polymorphism of the structure of SARS-CoV-2 nsp7

1. Yeongjoon Lee
2. Marco Tonelli
3. Mehdi Rahimi
4. Thomas K. Anderson
5. Robert N. Kirchdoerfer
6. Katherine Henzler-Wildman
7. Woonghee Lee

Reviewed by ScreenIT

Structure of mycobacterial CIII2CIV2 respiratory supercomplex bound to the tuberculosis drug candidate telacebec (Q203)

1. David J Yanofsky
2. Justin M Di Trani
3. Sylwia Król
4. Rana Abdelaziz
5. Stephanie A Bueler
6. Peter Imming
7. Peter Brzezinski
8. John L Rubinstein

• Curated by eLife

  Evaluation Summary:

  The manuscript by Yanofsky et al. describes the high-resolution structure of the CIII/CIV super-complex from Mycobacterium smegmatis bound to the anti-tuberculosis drug Q203 which is currently in clinical trials. The authors also provide biochemical data for inhibition of purified CIII/CIV and add important new information regarding the mechanism of Q203. This is an important contribution that will be of broad interest to the field of bioenergetic and tuberculosis, as telacebec represents one of few novel drug classes with potential for treatment of tuberculosis and other mycobacterial infections.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Structural basis of polyamine transport by human ATP13A2 (PARK9)

1. Sue Im Sim
2. Sören von Bülow
3. Gerhard Hummer
4. Eunyong Park

Reviewed by Biophysics Colab

An extended motif in the SARS-CoV-2 spike modulates binding and release of host coatomer in retrograde trafficking

1. Debajit Dey
2. Suruchi Singh
3. Saif Khan
4. Matthew Martin
5. Nicholas J. Schnicker
6. Lokesh Gakhar
7. Brian G. Pierce
8. S. Saif Hasan

Reviewed by ScreenIT

Structural and biochemical rationale for enhanced spike protein fitness in delta and kappa SARS-CoV-2 variants

1. James W. Saville
2. Dhiraj Mannar
3. Xing Zhu
4. Shanti S. Srivastava
5. Alison M. Berezuk
6. Jean-Philippe Demers
7. Steven Zhou
8. Katharine S. Tuttle
9. Inna Sekirov
10. Andrew Kim
11. Wei Li
12. Dimiter S. Dimitrov
13. Sriram Subramaniam

Reviewed by ScreenIT

Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptor

1. Tümay Capraz
2. Nikolaus F Kienzl
3. Elisabeth Laurent
4. Jan W Perthold
5. Esther Föderl-Höbenreich
6. Clemens Grünwald-Gruber
7. Daniel Maresch
8. Vanessa Monteil
9. Janine Niederhöfer
10. Gerald Wirnsberger
11. Ali Mirazimi
12. Kurt Zatloukal
13. Lukas Mach
14. Josef M Penninger
15. Chris Oostenbrink
16. Johannes Stadlmann

• Curated by eLife

  Evaluation Summary:

  This manuscript is of broad interest in light of the COVID-19 pandemic. Characterization of how glycosylation affects interactions between the viral Spike protein and ACE2 receptor can inform efforts to inhibit the SARS-CoV-2. The molecular modeling and functional analysis need to be improved.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife, ScreenIT

Enzymatic Beacons for Specific Sensing of Dilute Nucleic Acid**

1. Xiaoyu Zhang
2. Venubabu Kotikam
3. Eriks Rozners
4. Brian P. Callahan

Reviewed by ScreenIT