Biochemistry

DTX3L ubiquitin ligase ubiquitinates single-stranded nucleic acids

1. Emily L Dearlove
2. Chatrin Chatrin
3. Lori Buetow
4. Syed F Ahmed
5. Tobias Schmidt
6. Martin Bushell
7. Brian O Smith
8. Danny T Huang

• Curated by eLife

  eLife assessment

  This important study reports the discovery of a novel nucleotide ubiquitylation activity by the DTX3L E3 ligase. Solid evidence is presented for ubiquitin attachment to single-stranded oligonucleotides. This very interesting biochemical finding can be used as a starting point for studies to establish relevance in a physiological setting.

Reviewed by eLife

Evolutionarily divergent Mycobacterium tuberculosis CTP synthase filaments are under selective pressure

1. Eric M. Lynch
2. Yao Lu
3. Jin Ho Park
4. Lin Shao
5. Justin Kollman
6. E. Hesper Rego

Reviewed by PREreview

SIRT2-mediated ACSS2 K271 deacetylation suppresses lipogenesis under nutrient stress

1. Rezwana Karim
2. Wendi Teng
3. Cameron D Behram
4. Hening Lin

• Curated by eLife

  eLife assessment

  This useful study describes a role for acetylation in controlling the stability of acetyl-CoA synthetase 2, which converts acetate to acetyl-CoA for de novo lipid synthesis. While many aspects of the study are solid, some evidence supporting these findings is incomplete. Including direct demonstration of target deacetylation by sirtuin 2, revisiting statistical analyses, and confirming generalizability to adipocyte cell lines would further strengthen the study. This work will be of interest to researchers studying lipid metabolism and related diseases.

Reviewed by eLife

The deubiquitinase Ubp3/Usp10 constrains glucose-mediated mitochondrial repression via phosphate budgeting

1. Vineeth Vengayil
2. Shreyas Niphadkar
3. Swagata Adhikary
4. Sriram Varahan
5. Sunil Laxman

• Curated by eLife

  eLife assessment

  This study provides valuable insights into the regulation of metabolic flux between glycolysis and respiration in yeast, particularly focusing on the role of inorganic phosphate. The authors propose a novel mechanism involving Ubp3/Ubp10 that potentially mitigates the Crabtree effect, offering substantial, solid evidence through a variety of well-designed assays. This study could reshape our understanding of metabolic regulation with broad biological contexts.

Reviewed by eLife

Reassessing the substrate specificities of the major Staphylococcus aureus peptidoglycan hydrolases lysostaphin and LytM

1. Lina Antenucci
2. Salla Virtanen
3. Chandan Thapa
4. Minne Jartti
5. Ilona Pitkänen
6. Helena Tossavainen
7. Perttu Permi

• Curated by eLife

  eLife assessment

  This manuscript describes a valuable study aimed at identifying the substrate specificity of two cell wall hydrolases LSS and LytM in S. aureus. The authors show that LytM has a novel function of cleaving D-Ala-Gly instead of only Gly-Gly by using synthetic substrates and compelling NMR-based real-time kinetics measurements.

Reviewed by eLife

Intramolecular feedback regulation of the LRRK2 Roc G domain by a LRRK2 kinase-dependent mechanism

1. Bernd K Gilsbach
2. Franz Y Ho
3. Benjamin Riebenbauer
4. Xiaojuan Zhang
5. Giambattista Guaitoli
6. Arjan Kortholt
7. Christian Johannes Gloeckner

• Curated by eLife

  eLife assessment

  This valuable manuscript reports on the relationship between GTP hydrolysis parameters and kinase activity of LRRK2, which is associated with Parkinson's disease. The authors provide a detailed accounting of the catalytic efficiency of the ROC GTPase domain of pathogenic variants of LRRK2, in comparison with the wild-type enzyme. The authors propose that phosphorylation of T1343 inhibits kinase activity and influences monomer-dimer transitions, but the experimental evidence is currently incomplete.

Reviewed by eLife

Direct observation of fluorescent proteins in gels: A rapid, cost‐efficient, and quantitative alternative to immunoblotting

1. Matthieu Sanial
2. Ryan Miled
3. Marine Alves
4. Sandra Claret
5. Nicolas Joly
6. Véronique Proux‐Gillardeaux
7. Anne Plessis
8. Sébastien Léon

Reviewed by Review Commons

Mag-Net: Rapid enrichment of membrane-bound particles enables high coverage quantitative analysis of the plasma proteome

1. Christine C. Wu
2. Kristine A. Tsantilas
3. Jea Park
4. Deanna Plubell
5. Justin A. Sanders
6. Previn Naicker
7. Ireshyn Govender
8. Sindisiwe Buthelezi
9. Stoyan Stoychev
10. Justin Jordaan
11. Gennifer Merrihew
12. Eric Huang
13. Edward D. Parker
14. Michael Riffle
15. Andrew N. Hoofnagle
16. William S. Noble
17. Kathleen L. Poston
18. Thomas J. Montine
19. Michael J. MacCoss

Reviewed by PREreview

A unique cell division protein critical for the assembly of the bacterial divisome

1. Xiao Chu
2. Lidong Wang
3. Yiheng Zhu
4. Zhengshan Feng
5. Qingtian Guan
6. Lei Song
7. Zhaoqing Luo

• Curated by eLife

  eLife assessment:

  This useful study shows that the essential Acinetobacter baumannii gene Aeg1 likely plays an key role in cell division. The strength of the work is the discovery that the depletion of Aeg1 leads to cell filamentation and that gain-of-function mutations in cell division genes FtsB and FtsL rescue the lethality of Aeg1 depletion. However, Aeg1's localization pattern and its requirement for other division proteins' localizations require further characterization of the functionality of fluorescent fusion proteins, fluorescence images of higher quality, and improvements in statistic qualifications, leaving the study' evidence for Aeg1's exact role in cell division incomplete at this time. In conclusion, the critical role of Aeg1 in the assembly of the A. baumannii divisome has yet to be established unambiguously.

Reviewed by eLife, Arcadia Science

Activation of polycystin-1 signaling by binding of stalk-derived peptide agonists

1. Shristi Pawnikar
2. Brenda S Magenheimer
3. Keya Joshi
4. Ericka Nevarez-Munoz
5. Allan Haldane
6. Robin L Maser
7. Yinglong Miao

• Curated by eLife

  eLife assessment

  This joint computational/experimental study demonstrates the ability of synthetic peptides derived from the stalk-tethered agonist in Polycystin-1 (PC1) to re-activate signaling by a stalkless C-terminal fragment of PC1. The study is valuable as it discovered peptide agonists for PC1 and the integrated in vitro and in silico approach is potentially applicable to the analysis of related systems. Following the revision, the line of evidence presented in the current manuscript is considered convincing.

Reviewed by eLife