Biochemistry

A multi-hierarchical approach reveals d-serine as a hidden substrate of sodium-coupled monocarboxylate transporters

1. Pattama Wiriyasermkul
2. Satomi Moriyama
3. Masataka Suzuki
4. Pornparn Kongpracha
5. Nodoka Nakamae
6. Saki Takeshita
7. Yoko Tanaka
8. Akina Matsuda
9. Masaki Miyasaka
10. Kenji Hamase
11. Tomonori Kimura
12. Masashi Mita
13. Jumpei Sasabe
14. Shushi Nagamori

• Curated by eLife

  eLife assessment

  This study shows compelling evidence that the less common D-serine stereoisomer is transported in the kidney by the neutral amino acid transporter ASCT2 and that it is a non-canonical substrate for sodium-coupled monocarboxylate transporter SMCTs. With a multi-hierarchical approach, this important study further shows that Ischemia-Reperfusion Injury in the kidney causes a specific increment in renal reabsorption carried out, in part, by ASCT2.

Reviewed by eLife

Identification of an evolutionary conserved binding motif responsible for the recruitment of NMD factors to the UPF1 helicase

1. Irène Barbarin-Bocahu
2. Nathalie Ulryck
3. Amandine Rigobert
4. Nadia Ruiz Gutierrez
5. Laurence Decourty
6. Mouna Raji
7. Bhumika Garkhal
8. Hervé Le Hir
9. Cosmin Saveanu
10. Marc Graille

Reviewed by Review Commons

Caenorhabditis elegans Dicer acts with the RIG-I-like helicase DRH-1 and RDE-4 to cleave dsRNA

1. Claudia D Consalvo
2. Adedeji M Aderounmu
3. Helen M Donelick
4. P Joseph Aruscavage
5. Debra M Eckert
6. Peter S Shen
7. Brenda L Bass

• Curated by eLife

  eLife assessment

  To investigate the evolutionary relationship between the RNAi pathway and innate immunity, this valuable study uses biochemistry and structural biology to investigate the trimeric complex of Dicer-1, DRH-1 (a RIGI homologue), and RDE-4, which exists in C. elegans. The results described include rigorous kinetic analysis of the enzymatic activity of the complex and a moderate resolution cryo-EM structure. The results are convincing and add to the broader understanding of the evolution of antiviral defense.

Reviewed by eLife

Giardia intestinalis deoxyadenosine kinase has a unique tetrameric structure that enables high substrate affinity and makes the parasite sensitive to deoxyadenosine analogues

1. Farahnaz Ranjbarian
2. Karim Rafie
3. Kasturika Shankar
4. Sascha Krakovka
5. Staffan G. Svärd
6. Lars-Anders Carlson
7. Anders Hofer

Reviewed by Review Commons

Crosslinking by ZapD drives the assembly of short FtsZ filaments into toroidal structures in solution

1. Adrián Merino-Salomón
2. Jonathan Schneider
3. Leon Babl
4. Jan-Hagen Krohn
5. Marta Sobrinos-Sanguino
6. Tillman Schäfer
7. Juan R. Luque-Ortega
8. Carlos Alfonso
9. Mercedes Jiménez
10. Marion Jasnin
11. Petra Schwille
12. Germán Rivas

• Curated by eLife

  eLife assessment

  The formation of the Z-ring at the time of bacterial cell division interests researchers working towards understanding cell division across all domains of life. The manuscript by Jasnin et al reports the cryoET structure of toroid assembly formation of FtsZ filaments driven by ZapD as the cross linker. The findings are important and have the potential to open a new dimension in the field, but the evidence to support these exciting claims is currently inadequate.

Reviewed by eLife

CK-666 and CK-869 differentially inhibit Arp2/3 iso-complexes

1. LuYan Cao
2. Shaina Huang
3. Angika Basant
4. Miroslav Mladenov
5. Michael Way

Reviewed by Review Commons

Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro system

1. Fujun Zhou
2. Julie M Bocetti
3. Meizhen Hou
4. Daoming Qin
5. Alan G Hinnebusch
6. Jon R Lorsch

• Curated by eLife

  eLife assessment

  This is an important paper as it is the first to use a reconstituted translation system to study competition among mRNAs for the initiation machinery. Understanding the principles of the biochemistry of mRNA competition for initiation factors cannot be achieved without such a system. The authors provide compelling evidence that Ded1 is required for efficient initiation of highly structured mRNAs. The findings are significant and validate the in vitro reconstituted system by recapitulating the effects of in-vivo perturbations of translation initiation by Ded1 mutants.

Reviewed by eLife

Viral proteins activate PARIS-mediated tRNA degradation and viral tRNAs rescue infection

1. Nathaniel Burman
2. Svetlana Belukhina
3. Florence Depardieu
4. Royce A. Wilkinson
5. Mikhail Skutel
6. Andrew Santiago-Frangos
7. Ava B. Graham
8. Alexei Livenskyi
9. Anna Chechenina
10. Natalia Morozova
11. Trevor Zahl
12. William S. Henriques
13. Murat Buyukyoruk
14. Christophe Rouillon
15. Lena Shyrokova
16. Tatsuaki Kurata
17. Vasili Hauryliuk
18. Konstantin Severinov
19. Justine Groseille
20. Agnès Thierry
21. Romain Koszul
22. Florian Tesson
23. Aude Bernheim
24. David Bikard
25. Blake Wiedenheft
26. Artem Isaev

Reviewed by Rapid Reviews Infectious Diseases

Effects of phosphorylation on Drp1 activation by its receptors, actin, and cardiolipin

1. Ao Liu
2. Anna L. Hatch
3. Henry N. Higgs

Reviewed by Review Commons

Fascin-induced bundling protects actin filaments from disassembly by cofilin

1. Jahnavi Chikireddy
2. Léana Lengagne
3. Rémi Le Borgne
4. Catherine Durieu
5. Hugo Wioland
6. Guillaume Romet-Lemonne
7. Antoine Jégou

Reviewed by Review Commons