Biochemistry

  1. In vivo exchange of glucose and lactate between photoreceptors and the retinal pigment epithelium

    1. Daniel T Hass
    2. Elizabeth Giering
    3. John YS Han
    4. Celia M Bisbach
    5. Kriti Pandey
    6. Brian M Robbings
    7. Thomas O Mundinger
    8. Nicholas D Nolan
    9. Stephen H Tsang
    10. Neal S Peachey
    11. Nancy J Philp
    12. James B Hurley

    • Curated by eLife

      eLife Assessment

      This research is valuable as it investigates metabolic shuttling between photoreceptors and retinal pigment epithelium (RPE) using in vivo infusion techniques and mouse models. The authors find that the retina significantly relies on circulating glucose, with photoreceptors being the primary consumers of glucose, which is convincing. However, the study has incomplete evidence to support the claims that photoreceptors can use lactate as a fuel source, that lactate exported from photoreceptors is utilized by the RPE, and that lactate contributes to the TCA cycle in the RPE. These claims need substantial revision to include potential alternative explanations or perform key experiments.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity

  2. Unconventional structure and function of PHD domains from additional sex combs‐like proteins

    1. Cameron J. Reddington
    2. Annabel R. Walsh
    3. Torsten Kleffmann
    4. Christoph Göbl
    5. Peter D. Mace

    Reviewed by Review Commons

    3 evaluationsAppears in 1 listLatest version Latest activity

  3. Butyrolactol A potentiates caspofungin efficacy against resistant fungi via phospholipid flippase inhibition

    1. Xuefei Chen
    2. H. Diessel Duan
    3. Michael J. Hoy
    4. Kalinka Koteva
    5. Michaela Spitzer
    6. Allison K. Guitor
    7. Emily Puumala
    8. Aline A. Fiebig
    9. Guanggan Hu
    10. Bonnie Yiu
    11. Sommer Chou
    12. Zhuyun Bian
    13. Yeseul Choi
    14. Amelia Bing Ya Guo
    15. Wenliang Wang
    16. Sheng Sun
    17. Nicole Robbins
    18. Anna Floyd Averette
    19. Michael A. Cook
    20. Ray Truant
    21. Lesley T. MacNeil
    22. Eric D. Brown
    23. James W. Kronstad
    24. Brian K. Coombes
    25. Leah E. Cowen
    26. Joseph Heitman
    27. Huilin Li
    28. Gerard D. Wright

    Reviewed by PREreview

    1 evaluationAppears in 2 listsLatest version Latest activity

  4. Enzymatic protein fusions with 100% product yield

    1. Adrian CD Fuchs

    • Curated by eLife

      eLife Assessment

      This revision of important work is a versatile addition to the chemical protein modifications and bioconjugation toolbox in synthetic biology. The technology developed cleverly uses Connectase to irreversibly fuse proteins of interest together so they can be studied in their native context, with compelling well-controlled data showing the technique works for various protein partners. This work will help multiple fields to explore multi-function constructs in basic synthetic biology. This work will also be of interest to those studying fusion oncoproteins commonly expressed in various human pathologies.

    Reviewed by eLife

    8 evaluationsAppears in 1 listLatest version Latest activity

  5. Hepatic HKDC1 deletion alleviates western diet-induced MASH in mice

    1. Kai Xu
    2. Irene Corona-Avila
    3. María Dolores Frutos
    4. María Ángeles Núñez-Sánchez
    5. Dhruvi Makhanasa
    6. Pratham Viral Shah
    7. Grace Guzman
    8. Bruno Ramos-Molina
    9. Medha Priyadarshini
    10. Md. Wasim Khan

    Reviewed by PREreview, Arcadia Science

    6 evaluationsAppears in 2 listsLatest version Latest activity

  6. Protein codes promote selective subcellular compartmentalization

    1. Henry R. Kilgore
    2. Itamar Chinn
    3. Peter G. Mikhael
    4. Ilan Mitnikov
    5. Catherine Van Dongen
    6. Guy Zylberberg
    7. Lena Afeyan
    8. Salman F. Banani
    9. Susana Wilson-Hawken
    10. Tong Ihn Lee
    11. Regina Barzilay
    12. Richard A. Young

    Reviewed by Arcadia Science

    1 evaluationAppears in 1 listLatest version Latest activity

  7. Allosteric inhibition of trypanosomatid pyruvate kinases by a camelid single-domain antibody

    1. Joar Esteban Pinto Torres
    2. Mathieu Claes
    3. Rik Hendrickx
    4. Meng Yuan
    5. Natalia Smiejkowska
    6. Pieter Van Wielendaele
    7. Aysima Hacisuleyman
    8. Hans De Winter
    9. Serge Muyldermans
    10. Paul AM Michels
    11. Malcolm D Walkinshaw
    12. Wim Versées
    13. Guy Caljon
    14. Stefan Magez
    15. Yann G-J Sterckx

    • Curated by eLife

      eLife Assessment

      This work presents valuable data demonstrating that a camelid single-domain antibody can selectively inhibit a key glycolytic enzyme in trypanosomes via an allosteric mechanism. The claim that this information can be exploited for the design of novel chemotherapeutics is solid but limited by the modest effects on parasite growth, as well as the lack of evidence for cellular target engagement in vivo.

    Reviewed by eLife

    9 evaluationsAppears in 1 listLatest version Latest activity

  8. Proximity Interactome analyses unveil novel regulators of IRE1α canonical signaling

    1. Simon Le Goupil
    2. Céline Philippe
    3. Hadrien Laprade
    4. Mathéo Lode
    5. Rachel Boniface
    6. Diana Pelizzari-Raymundo
    7. Kurt Dejgaard
    8. Gregor Jansen
    9. Celia Limia
    10. Claudio Hetz
    11. S. Jalil Mahdizadeh
    12. Luc Negroni
    13. Marc Aubry
    14. Jean-Ehrland Ricci
    15. Leif A. Eriksson
    16. Eric Chevet

    Reviewed by Review Commons

    4 evaluationsAppears in 1 listLatest version Latest activity

  9. Molecular determinants of Neu5Ac binding to a tripartite ATP independent periplasmic (TRAP) transporter

    1. Parveen Goyal
    2. KanagaVijayan Dhanabalan
    3. Mariafrancesca Scalise
    4. Rosmarie Friemann
    5. Cesare Indiveri
    6. Renwick CJ Dobson
    7. Kutti R Vinothkumar
    8. Subramanian Ramaswamy

    • Curated by eLife

      eLife Assessment

      This valuable work provides novel insights into the substrate binding mechanism of a tripartite ATP-independent periplasmic (TRAP) transporter, which may be helpful for the development of specific inhibitors. The structural analysis is convincing, but additional work will be required to establish the transport mechanism as well as well as binding sites for all ligands. This study will be of interest to the membrane transport and bacterial biochemistry communities.

    Reviewed by eLife

    12 evaluationsAppears in 2 listsLatest version Latest activity

  10. Reduction of DNA Topoisomerase Top2 Reprograms the Epigenetic Landscape and Extends Health and Life Span Across Species

    1. Man Zhu
    2. Meng Ma
    3. Lunan Luo
    4. Feiyang Li
    5. Jiashun Zheng
    6. Yan Pan
    7. Lu Yang
    8. Ying Xiao
    9. Ziyan Wang
    10. Bo Xian
    11. Yi Zheng
    12. Hao Li
    13. Jing Yang

    • Curated by eLife

      eLife Assessment

      Building on earlier evidence that Topoisomerase 2 depletion can extend yeast lifespan, the authors present convincing data demonstrating that reduced Top2 levels confer significant longevity benefits in C. elegans and mice. They show that these benefits encompass not only extended survival but also improved healthspan and mitigation of aging phenotypes, supported by robust transcriptomic, epigenetic, and histological analyses. While the exact mechanistic underpinnings-such as the potential connection to classical aging pathways and the selective down-regulation of genes with active promoters-remain to be fully elucidated, the breadth of these important findings strongly indicates a conserved role for Top2 in aging regulation.

    Reviewed by eLife

    3 evaluationsAppears in 1 listLatest version Latest activity
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