Biochemistry

Engineering cardiolipin binding to an artificial membrane protein reveals determinants for lipid-mediated stabilization

1. Mia L Abramsson
2. Robin A Corey
3. Jan Škerle
4. Louise J Persson
5. Olivia Andén
6. Abraham O Oluwole
7. Rebecca J Howard
8. Erik Lindahl
9. Carol V Robinson
10. Kvido Strisovsky
11. Erik G Marklund
12. David Drew
13. Phillip J Stansfeld
14. Michael Landreh

• Curated by eLife

  eLife Assessment

  Cardiolipin is known to play an important role in modulating the assembly and function of membrane proteins in bacterial and mitochondrial membranes. In this study, authors convincingly define the molecular determinants of cardiolipin binding on de novo-designed and native membrane proteins combining the coarse-grained molecular dynamics simulation with the state-of-the-art experimental approaches such as native mass spectrometry and cryogenic electron microscopy. The major findings in this study, which are the identification of degenerate cardiolipin binding motifs and their role in membrane protein stability and activity, will provide much needed insight into the still poorly understood nature of protein-cardiolipin interactions.

Reviewed by eLife

Interaction hierarchy among Cdv proteins drives recruitment to membrane necks

1. Nicola De Franceschi
2. Alberto Blanch-Jover
3. Cees Dekker

• Curated by eLife

  eLife Assessment

  This valuable study investigates how the proteins of the Cdv division system in Metallosphaera sedula archaea sequentially interact with curved membranes in vitro, extending our understanding of this reduced ESCRT-like machinery. While the data support key aspects of protein recruitment and membrane remodeling, missing controls and statistical analysis information, unaddressed discrepancies, and limitations in recapitulating native geometry leave the data incomplete to fully support the proposed conclusions. The work will be of interest to evolutionary and synthetic biologists as membrane biophysicists but would benefit from additional experiments and a more cautious interpretation of results.

Reviewed by eLife

Simultaneous polyclonal antibody sequencing and epitope mapping by cryo electron microscopy and mass spectrometry – a perspective

1. Douwe Schulte
2. Marta Šiborová
3. Lukas Käll
4. Joost Snijder

• Curated by eLife

  eLife Assessment

  This manuscript describes a method using EM polyclonal epitope mapping to help elucidate endogenous antibodies. Overall the work described is interesting and the contribution will be of use to the field that is expected to only increase in impact and value over time. The significance of the work is considered valuable and the strength of evidence to support its findings is considered solid.

Reviewed by eLife

Membrane mimetic thermal proteome profiling (MM-TPP) towards mapping membrane protein-ligand dynamics

1. Rupinder Singh Jandu
2. Mohammed Al-Seragi
3. Hiroyuki Aoki
4. Mohan Babu
5. Franck Duong van Hoa

• Curated by eLife

  eLife Assessment

  This useful study introduces the peptidisc-TPP approach as a promising solution to challenges in membrane proteomics, enabling thermal proteome profiling in a detergent-free system. While the concept is innovative and holds significant potential, the demonstration of its utility and validation remains incomplete. The method presents a strong foundation for broader applications in identifying physiologically and pharmacologically relevant membrane protein-ligand interactions.

Reviewed by eLife

Elevated Ubiquitin Phosphorylation by PINK1 Contributes to Proteasomal Impairment and Promotes Neurodegeneration

1. Cong Chen
2. Tong-Yao Gao
3. Hua-Wei Yi
4. Yi Zhang
5. Tong Wang
6. Zhi-Lin Lou
7. Tao-Feng Wei
8. Yun-Bi Lu
9. Ting-Ting Li
10. Chun Tang
11. Wei-Ping Zhang

• Curated by eLife

  eLife Assessment

  The useful manuscript presents interesting findings in the field of neurodegenerative diseases by highlighting the dual role of phosphorylated ubiquitin (pUb) in cellular proteostasis and neurotoxicity. However, some claims for discovery are supported by unconvincing and incomplete evidence that requires further validation. The poor quality of key immunofluorescent images and questionable quantification analysis raise technical concerns.

Reviewed by eLife

Enzymatic access to the rare ΔUA (1→4) Glc 3, 6, N-sulfated heparin disaccharide, implications for heparin quality control

1. T. K. Kandola
2. C. J. Mycroft-West
3. M. L. Andrade De Lima
4. A. Turner
5. C. Gardini
6. E. Urso
7. G.J. Miller
8. A. Bisio
9. E. A. Yates
10. M. Guerrini
11. L. Wu
12. M. A. Skidmore

Reviewed by preLights

Curcuma longa Essential Oil Topically Mitigates Inflammatory Markers in Acetone-Induced Atopic Dermatitis in Wistar Albino Rats

1. Nathiim Namale
2. Deusdedit Tusubira
3. Kenneth Male
4. Angela Mumbua Musyoka
5. Patrick Maduabuchi Aja

Reviewed by Arcadia Science

Hepatic HKDC1 Deletion Alleviates Western Diet-Induced MASH in Mice

1. Kai Xu
2. Irene Covila-Corona
3. María Dolores Frutos
4. María Ángeles Núñez-Sánchez
5. Dhruvi Makhanasa
6. Pratham Viral Shah
7. Grace Guzman
8. Bruno Ramos-Molina
9. Medha Priyadarshini
10. Md. Wasim Khan

Reviewed by Arcadia Science

Paradoxical dominant negative activity of an immunodeficiency-associated activating PIK3R1 variant

1. Patsy R Tomlinson
2. Rachel Knox
3. Olga Perisic
4. Helen C Su
5. Gemma V Brierley
6. Roger L Williams
7. Robert K Semple

• Curated by eLife

  eLife Assessment

  This important study reports on PI3KR mutations and a paradoxical mechanism of PI3KR signaling. The strength of evidence for the study is mostly convincing, as conclusions are supported by a variety of mutational strategies and cellular systems to look at interactions among signaling pathways.

Reviewed by eLife

The conserved biochemical activity and function of an early metazoan phosphatidylinositol 5 phosphate 4-kinase regulates growth and development

1. Harini Krishnan
2. Suhail Muzaffar
3. Sanjeev Sharma
4. Visvanathan Ramya
5. Avishek Ghosh
6. Ramanathan Sowdhamini
7. Padinjat Raghu

Reviewed by Review Commons