1. Recognition of the Nucleotide-Dependent Feature Facilitates the Microtubule End-Binding of MCAK

    This article has 4 authors:
    1. Wei Chen
    2. Yin-Long Song
    3. Jian-Feng He
    4. Xin Liang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This work presents valuable new information on the microtubule-binding mode of the microtubule kinesin-13, MCAK, the authors use quantitative single-molecule studies to propose that MCAK preferentially binds to a GDP-Pi-tubulin portion of the microtubule end. However, the evidence provided to support this claim remains incomplete and would benefit from more rigorous methodology particularly the diffraction limited experiments do not provide sufficient spatial resolution to support the authors' conclusions. In addition, a more through discussion of the existing literature would further strengthen the manuscript.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 1 listLatest version Latest activity
  2. Coupled equilibria of dimerization and lipid binding modulate SARS Cov 2 Orf9b interactions and interferon response

    This article has 11 authors:
    1. CJ San Felipe
    2. Jyoti Batra
    3. Monita Muralidharan
    4. Shivali Malpotra
    5. Durga Anand
    6. Rachel Bauer
    7. Kliment A Verba
    8. Danielle L Swaney
    9. Nevan J Krogan
    10. Michael Grabe
    11. James S Fraser
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This fundamental study demonstrates that lipid binding can regulate the dimerization state of the SARS-CoV2 Orf9b protein. The data from biophysical and cellular experiments along with mathematical modeling are compelling. This paper is broadly relevant to those studying coupled equilibria across all aspects of biology.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  3. Chronobot: Deep learning guided time-resolved cryo-EM captures molecular choreography of RecA in homology search

    This article has 8 authors:
    1. Märt-Erik Mäeots
    2. Simon Tupin
    3. Mohammad M. N. Esfahani
    4. Juan B. Rodriguez Molina
    5. Julie A. Clapperton
    6. Aran Amin
    7. Albane Imbert
    8. Radoslav I. Enchev

    Reviewed by PREreview

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  4. Crosslinking by ZapD drives the assembly of short FtsZ filaments into toroidal structures in solution

    This article has 12 authors:
    1. Adrián Merino-Salomón
    2. Jonathan Schneider
    3. Leon Babl
    4. Jan-Hagen Krohn
    5. Marta Sobrinos-Sanguino
    6. Tillman Schäfer
    7. Juan R Luque-Ortega
    8. Carlos Alfonso
    9. Mercedes Jiménez
    10. Marion Jasnin
    11. Petra Schwille
    12. Germán Rivas
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The formation of the Z-ring at the time of bacterial cell division interests researchers working towards understanding cell division across all domains of life. The manuscript by Jasnin et al reports the cryoET structure of toroid assembly formation of FtsZ filaments driven by ZapD as the cross linker. The findings are important and have the potential to open a new dimension in the field, and the evidence to support these exciting claims is solid.

    Reviewed by eLife

    This article has 13 evaluationsAppears in 1 listLatest version Latest activity
  5. Concatenated Modular BK Channel Constructs Reveal Divergent Stoichiometry in Gating Control by LRRC26 (γ1), Pore, and Selectivity Filter

    This article has 3 authors:
    1. Guanxing Chen
    2. Qin Li
    3. Jiusheng Yan
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      In this important contribution, Yan and colleagues describe a powerful and compelling strategy to generate concatamers of the BK channel and their fusion constructs with the auxiliary gamma subunits, which allows exploring contributions of individual subunits of the tetrameric channel to its gating and the study of heteromeric channel complexes of defined composition. Distinct examples are presented, which illustrate great diversity in the stoichiometric control of BK channel gating, depending on the site and nature of molecular perturbations. The molecular approaches could be extended to other membrane proteins whose N and C termini face opposite sides of the membrane.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  6. General Trends in the Calnexin-Dependent Expression and Pharmacological Rescue of Clinical CFTR Variants

    This article has 13 authors:
    1. Austin Tedman
    2. John A Olson
    3. Minsoo Kim
    4. Catherine Foye
    5. JaNise J Jackson
    6. Eli F McDonald
    7. Andrew G McKee
    8. Karen Noguera
    9. Charles P Kuntz
    10. Jens Meiler
    11. Kathryn Oliver
    12. Lars Plate
    13. Jonathan P Schlebach
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study systematically investigates the effects of calnexin, an endoplasmic reticulum chaperone, on the drug response of approximately 230 disease-causing variants of the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Through deep mutational scanning, interactome profiling, and functional assays, the findings provide convincing evidence that calnexin significantly influences both CFTR expression and the efficacy of corrector drugs in a variant-specific manner. These insights advance our understanding of how cellular quality control machinery shapes the pharmacological responsiveness of CFTR variants, which are broadly relevant for researchers in protein folding and genetic disease therapeutics.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  7. A miniaturized, high-throughput buffer-centric method for protein solubility screening

    This article has 4 authors:
    1. Adrian Svoboda
    2. Marina Molineris
    3. Tomáš Pluskal
    4. Teo Hebra

    Reviewed by Arcadia Science

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  8. Allosteric modulation of dimeric GPR3 by ligands in the dimerization interface

    This article has 7 authors:
    1. Zeming Qiu
    2. Wei Wang
    3. Yingying Nie
    4. Junxiang Lin
    5. Beimeng Zhang
    6. Haonan Xing
    7. Sanduo Zheng
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      Qiu et al. present multiple dimeric structures of GPR3, which reveal the binding mode of the inverse agonist AF64394. The findings provide important insights into the regulation of GPCR3 and potentially other related orphan GPCRs. The authors present convincing evidence of their claims through thoughtful analysis of their cryo-EM structures, mutagenesis, and cell-based assays. This work will be of interest to GPCR investigators, especially those studying the signaling of orphan receptors.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  9. Inherited CDA-I disease: anemia-associated mutations disrupt CDIN1-Codanin1 complex

    This article has 9 authors:
    1. Martin Stojaspal
    2. Tomáš Brom
    3. Ivona Nečasová
    4. Tomáš Janovič
    5. Pavel Veverka
    6. Naina Verma
    7. Lukáš Uhrík
    8. Lenka Hernychova
    9. Ctirad Hofr

    Reviewed by Review Commons

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  10. Amyloid-β fibrils accumulated in preeclamptic placentas suppress syncytialization of cytotrophoblasts

    This article has 14 authors:
    1. Kaho Nishioka
    2. Midori Ikezaki
    3. Naoyuki Iwahashi
    4. Miyu Arakawa
    5. Momo Fukushima
    6. Noa Mori
    7. Mika Mizoguchi
    8. Yuko Horiuchi-Tanizaki
    9. Megumi Fujino
    10. Takami Tomiyama
    11. Yoshito Ihara
    12. Kenji Uchimura
    13. Kazuhiko Ino
    14. Kazuchika Nishitsuji

    Reviewed by Review Commons

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
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