Biochemistry

RNF13 is a novel interactor of iduronate 2-sulfatase that modifies its glycosylation and maturation

1. Valérie C. Cabana
2. Antoine Y. Bouchard
3. Audrey M. Sénécal
4. Laurent Cappadocia
5. Marc P. Lussier

Reviewed by Arcadia Science

Aspartate transaminases are required for blood development

1. Narges Pourmandi
2. Greggory Myers
3. Arjun Jha
4. Kelsey Temprine
5. Amanda Sankar
6. Nupur K. Das
7. Ridwana Khan
8. Siva Kumar Natarajan
9. Cristina Castillo
10. Peter Sajjakulnukit
11. Noah S. Nelson
12. Matthew D. Perricone
13. Indrani Talukder
14. Aaron D. denDekker
15. Lin Lin
16. Dominik Awad
17. Wesley Huang
18. Lei Yu
19. Navdeep S. Chandel
20. Rami Khoriaty
21. Yatrik M. Shah
22. Costas A. Lyssiotis

Reviewed by preLights

Gut Microbe-Derived Trimethylamine Shapes Circadian Rhythms Through the Host Receptor TAAR5

1. Kala K Mahen
2. William J Massey
3. Danny Orabi
4. Amanda L Brown
5. Thomas C Jaramillo
6. Amy C Burrows
7. Anthony J Horak
8. Sumita Dutta
9. Marko Mrdjen
10. Nour Mouannes
11. Venkateshwari Varadharajan
12. Lucas J Osborn
13. Xiayan Ye
14. Dante M Yarbrough
15. Treg Grubb
16. Natalie Zajczenko
17. Rachel Hohe
18. Rakhee Banerjee
19. Pranavi Linga
20. Dev Laungani
21. Adeline M Hajjar
22. Naseer Sangwan
23. Mohammed Dwidar
24. Jennifer A Buffa
25. Garth R Swanson
26. Zeneng Wang
27. J Mark Brown

• Curated by eLife

  eLife Assessment

  This study presents an important finding linking the bacterial metabolite trimethylamine and its receptor to circadian rhythms and olfaction. The current evidence supporting the claims of the authors is compelling. This work will be of broad interest to researchers interested in nutrition, microbial metabolism, circadian rhythms, and host-microbiome interactions.

Reviewed by eLife

ATP-independent phosphate recycling on AGC kinase activation loops induced by alkali metal ions

1. Koji Kubouchi
2. Hideyuki Mukai

• Curated by eLife

  eLife Assessment

  AGC kinases, such as PKN1, are regulated by activation loop phosphorylation. This paper reports that exposing cells to high concentrations of monovalent cations induces rapid activation loop dephosphorylation, with rapid re-phosphorylation when physiological salt is restored. Re-phosphorylation is apparently independent of ATP or candidate kinases, and the paper presents an extraordinary and unconventional mechanism involving phosphate exchange between the activation loop and an unknown acceptor molecule. The findings are intriguing and the approach is logical, but the evidence is incomplete and the significance unclear until the biochemical mechanism is identified.

Reviewed by eLife

Viral proteins activate PARIS-mediated tRNA degradation and viral tRNAs rescue infection

1. Nathaniel Burman
2. Svetlana Belukhina
3. Florence Depardieu
4. Royce A. Wilkinson
5. Mikhail Skutel
6. Andrew Santiago-Frangos
7. Ava B. Graham
8. Alexei Livenskyi
9. Anna Chechenina
10. Natalia Morozova
11. Trevor Zahl
12. William S. Henriques
13. Murat Buyukyoruk
14. Christophe Rouillon
15. Lena Shyrokova
16. Tatsuaki Kurata
17. Vasili Hauryliuk
18. Konstantin Severinov
19. Justine Groseille
20. Agnès Thierry
21. Romain Koszul
22. Florian Tesson
23. Aude Bernheim
24. David Bikard
25. Blake Wiedenheft
26. Artem Isaev

Reviewed by Rapid Reviews Infectious Diseases

Kinesin-1 conformational dynamics are controlled by a cargo-sensitive TPR switch

1. Shivam Shukla
2. Jessica A Cross
3. Monika Kish
4. Sathish KN Yadav
5. Johannes F Weijman
6. Laura O’Regan
7. Judith Mantell
8. Ufuk Borucu
9. Xiyue Leng
10. Christiane Schaffitzel
11. Jonathan J Phillips
12. Derek N Woolfson
13. Mark P Dodding

• Curated by eLife

  eLife Assessment

  The manuscript by Shukla et al. provides important mechanistic insights into kinesin-1 autoinhibition and cargo-mediated activation. Using a convincing combination of protein engineering, computational modeling, biophysical assays, HDX-MS, and electron microscopy, the authors reveal how cargo binding induces an allosteric transition that propagates to the motor domains and enhances MAP7 binding. Despite limitations arising from conformational heterogeneity and structural resolution, the study presents a unified mechanism for kinesin-1 activation that will be of broad interest to the motor protein, structural biology, and cell biology communities.

Reviewed by eLife

UV irradiation alters TFAM binding to mitochondrial DNA

1. Dillon E King
2. Emily E Beard
3. Matthew J Satusky
4. Ian Ryde
5. Alex George
6. Caitlin Johnson
7. Emma L Dolan
8. Yuning Zhang
9. Wei Zhu
10. Hunter Wilkins
11. Evan Corden
12. Susan K Murphy
13. Dorothy Erie
14. Raluca Gordân
15. Joel N Meyer

• Curated by eLife

  eLife Assessment

  Mitochondrial DNA (mtDNA) exhibits a degree of resistance to mutagenesis under genotoxic stress, and this study on the mitochondrial Transcription Factor A (TFAM) presents valuable data concerning the possible mechanisms involved. The presented data are solid, technically rigorous, and consistent with established literature findings. The experiments are well-executed, providing reliable evidence on the change of TFAM-DNA interactions following UVC irradiation. However, the evidence is inadequate to support the primary claims.

Reviewed by eLife

A high-resolution analysis of arrestin2 interactions responsible for CCR5 endocytosis

1. Ivana Petrovic
2. Samit Desai
3. Polina Isaikina
4. Layara Akemi Abiko
5. Anne Spang
6. Stephan Grzesiek

• Curated by eLife

  eLife Assessment

  The authors investigate arrestin2-mediated CCR5 endocytosis in the context of clathrin and AP2 contributions. Using an extensive set of NMR experiments, and supported by microscopy and other biophysical assays, the authors provide compelling data on the roles of AP2 and clathrin in CCR5 endocytosis. This important work will appeal to an audience beyond those studying chemokine receptors, including those studying GPCR regulation and trafficking. The distinct role of AP2 and not clathrin will be of particular interest to those studying GPCR internalization mechanisms.

Reviewed by eLife

Aggregation-Dependent Epitope Sequence and Modification Fingerprints of Anti-Aβ Antibodies

1. Ivan Talucci
2. Timon Leske
3. Hans-Wolfgang Klafki
4. Mohammed Mehedi Hassan
5. Annik Steiert
6. Barbara Morgado
7. Sebastian Bothe
8. Lars van Werven
9. Thomas Liepold
10. Jochen Walter
11. Hermann Schindelin
12. Jens Wiltfang
13. Oliver Wirths
14. Olaf Jahn
15. Hans Michael Maric

• Curated by eLife

  eLife Assessment

  Antibodies that selectively bind distinct amyloid-beta variants are vital tools for Alzheimer's disease research. This valuable manuscript aims to delineate the epitope specificity in a panel of anti-amyloid-beta antibodies, including some with clinical relevance. The experiments were rigorously conducted, employing an interesting combination of established and state-of-the-art methodologies, yielding convincing findings.

Reviewed by eLife

Investigation of All Disease-Relevant Lysine Acetylation Sites in α-Synuclein Enabled by Non-canonical Amino Acid Mutagenesis

1. Marie Shimogawa
2. Ming-Hao Li
3. Grace Shin Hye Park
4. Jennifer Ramirez
5. Hudson Lee
6. Paris R Watson
7. Swati Sharma
8. Zongtao Lin
9. Chao Peng
10. Virginia M.-Y Lee
11. Benjamin A Garcia
12. David W Christianson
13. Elizabeth Rhoades
14. David Eliezer
15. E James Petersson

• Curated by eLife

  eLife Assessment

  This valuable work provides new insights into the role of lysine acetylation of alpha-synuclein, the protein involved in Parkinson's Disease. The evidence is mostly solid, but the claims around the potential disease relevance based on seeding assays and structural work need to be toned down, or else supported by additional experimental evidence. Overall, the work will be of interest to researchers in the fields of protein biophysics and post-translational modifications, as well as Parkinson's Disease.

Reviewed by eLife