Biochemistry

Force transmission through the inner kinetochore is enhanced by centromeric DNA sequences

1. Elise Miedlar
2. Grace E Hamilton
3. Samuel R Witus
4. Sara Gonske
5. Michael Riffle
6. Alex Zelter
7. Rachel E Klevit
8. Charles L Asbury
9. Yoana N Dimitrova
10. Trisha N Davis

• Curated by eLife

  eLife Assessment

  Centromeres are specific sites on chromosomes that are essential for mitosis and genome fidelity. This valuable research advance builds upon previous studies to convincingly show that the centromere-histone core contributes to force transduction through the kinetochore. The centromere mainly strengthens one of the two paths of force transduction, influenced by the centromeric DNA sequence. The mechanism underlying this phenomenon will be an exciting future avenue of research, given that centromeric DNAs are not conserved. This work will be of interest to those studying cell division and chromosome segregation.

Reviewed by eLife

Trifluoroacetate reduces plasma lipid levels and the development of atherosclerosis in mice

1. Wei Tang
2. Audrey S. Black
3. Romana Moench
4. Katayoon Marzban
5. Juan Antonio Raygoza Garay
6. James J. Zheng
7. Louis Conway
8. Antonio F. M. Pinto
9. Christopher G. Parker
10. Alan Saghatelian
11. Luke J. Leman
12. M. Reza Ghadiri

Reviewed by PREreview

Heterooligomerization drives structural plasticity of eukaryotic peroxiredoxins

1. Jannik Zimmermann
2. Lukas Lang
3. Julia Malo Pueyo
4. Mareike Riedel
5. Khadija Wahni
6. Dylan Stobbe
7. Christopher Lux
8. Steven Janvier
9. Didier Vertommen
10. Svenja Lenhard
11. Frank Hannemann
12. Helena Castro
13. Ana Maria Tomas
14. Johannes M. Herrmann
15. Armindo Salvador
16. Timo Mühlhaus
17. Jan Riemer
18. Joris Messens
19. Marcel Deponte
20. Bruce Morgan

Reviewed by PREreview

Mycobacterial EsxG·EsxH (TB9.8·TB10.4) peptides as a subunit vaccine to booster BCG vaccination in an experimental model of pulmonary Tuberculosis

1. Constanza Estefania Martínez-Olivares
2. Vasti Lozano-Ordaz
3. Dulce Mata-Espinosa
4. Jorge Alberto Barrios-Payán
5. Ángel Elías Ortiz-Cabrera
6. Yadira Rocio Rodríguez-Miguez
7. Rogelio Hernández-Pando

Reviewed by PREreview

Stabilization of GTSE1 by cyclin D1-CDK4/6-mediated phosphorylation promotes cell proliferation: relevance in cancer prognosis

1. Nelson García-Vázquez
2. Tania J González-Robles
3. Ethan Lane
4. Daria Spasskaya
5. Qingyue Zhang
6. Marc Kerzhnerman
7. YeonTae Jeong
8. Marta Collu
9. Daniele Simoneschi
10. Kelly V Ruggles
11. Gergely Rona
12. Sharon Kaisari
13. Michele Pagano

• Curated by eLife

  eLife Assessment

  In this article, García-Vázquez et al. report valuable findings demonstrating that G2 and S phases expressed protein 1 (GTSE1), is a previously unappreciated non-pocket substrate of the cyclin D/cyclin-dependent kinase (CDK) 4/6 axis. The authors provide convincing evidence showing that this mechanism is triggered in pathological states in which cyclin D levels are elevated (e.g., cancer). Overall, this study holds a promise to improve understanding of the mechanisms underpinning cell cycle progression including its dysregulation in neoplasia and may thus be of broad interest to researchers belonging to diverse biomedical disciplines ranging from cancer research to cell biology.

Reviewed by eLife

Engineering cardiolipin binding to an artificial membrane protein reveals determinants for lipid-mediated stabilization

1. Mia L Abramsson
2. Robin A Corey
3. Jan Škerle
4. Louise J Persson
5. Olivia Andén
6. Abraham O Oluwole
7. Rebecca J Howard
8. Erik Lindahl
9. Carol V Robinson
10. Kvido Strisovsky
11. Erik G Marklund
12. David Drew
13. Phillip J Stansfeld
14. Michael Landreh

• Curated by eLife

  eLife Assessment

  Cardiolipin is known to play an important role in modulating the assembly and function of membrane proteins in bacterial and mitochondrial membranes. Here, authors convincingly define the molecular determinants of cardiolipin binding on de novo-designed and native membrane proteins combining the coarse-grained molecular dynamics simulation with the state-of-the-art experimental approaches such as native mass spectrometry and cryogenic electron microscopy. The major findings in this study, which are the identification of degenerate cardiolipin binding motifs, the characterization of their dynamic features, and the role in membrane protein stability and activity, will provide much needed insight into the still poorly understood nature of protein-cardiolipin interactions.

Reviewed by eLife

Crystal structure and catalytic mechanism of PL35 family glycosaminoglycan lyases with an ultrabroad substrate spectrum

1. Lin Wei
2. Hai-Yan Cao
3. Ruyi Zou
4. Min Du
5. Qingdong Zhang
6. Danrong Lu
7. Xiangyu Xu
8. Yingying Xu
9. Wenshuang Wang
10. Xiu-Lan Chen
11. Yu-Zhong Zhang
12. Fuchuan Li

• Curated by eLife

  eLife Assessment

  This useful manuscript reports on the crystal structures of two glycosaminoglycan (GAG) lyases from the PL35 family, along with in vitro enzyme activity assays and comprehensive structure-guided mutagenesis. The authors have addressed key concerns by incorporating additional docking analyses, validating the role of His188 in alginate degradation, and providing ICP-MS data to examine Mn²⁺ binding. While these improvements enhance the study, the study is incomplete due to the lack of enzyme-substrate complex structures and reliance on modeling which still limit mechanistic insight. Nonetheless, the revised manuscript presents a more complete analysis that will be of interest to specialists in carbohydrate-active enzymes.

Reviewed by eLife

A stress-responsive p38 signaling axis in choanoflagellates

1. Florentine U. Rutaganira
2. Maxwell C. Coyle
3. Maria H.T. Nguyen
4. Iliana Hernandez
5. Alex P. Scopton
6. Arvin C. Dar
7. Nicole King

Reviewed by Life Science Editors Foundation, Arcadia Science

Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing

1. Jiale Zhou
2. Ding Zhao
3. Jinze Li
4. Deqiang Kong
5. Xiangrui Li
6. Renquan Zhang
7. Yuru Liang
8. Xun Gao
9. Yuqiang Qian
10. Di Wang
11. Jiahui Chen
12. Liangxue Lai
13. Yang Han
14. Zhanjun Li

• Curated by eLife

  eLife Assessment

  This potentially useful study introduces an orthogonal approach for detecting RNA modification, without chemical modification of RNA, which often results in RNA degradation and therefore loss of information. Compared to previous versions, the most recent one is improved and sufficiently aligned with the standards of the field to merit consideration by the research community, making the evidence solid according to said standards. Nevertheless, uncertainty regarding false positive and false negative rates remains, as it does for some of the alternative approaches. With more rigorous validation, the approach might be of particular interest for sites in RNA molecules where modifications are rare.

Reviewed by eLife

Simultaneous polyclonal antibody sequencing and epitope mapping by cryo electron microscopy and mass spectrometry – a perspective

1. Douwe Schulte
2. Marta Šiborová
3. Lukas Käll
4. Joost Snijder

• Curated by eLife

  eLife Assessment

  The paper addresses the problem of optimising the mapping of serum antibody responses against a known antigen. The manuscript describes a method using EM polyclonal epitope mapping to help elucidate endogenous antibodies. The work is interesting and valuable to the fields of immunology and serology, and the strength of evidence to support its findings is considered solid.

Reviewed by eLife