Biochemistry

Stabilization of GTSE1 by cyclin D1-CDK4/6 promotes cell proliferation: relevance in cancer prognosis

1. Nelson García-Vázquez
2. Tania J González-Robles
3. Ethan Lane
4. Daria Spasskaya
5. Qingyue Zhang
6. Marc Kerzhnerman
7. YeonTae Jeong
8. Marta Collu
9. Daniele Simoneschi
10. Kelly V Ruggles
11. Gergely Rona
12. Michele Pagano
13. Sharon Kaisari

• Curated by eLife

  eLife assessment

  In this valuable study, García-Vázquez et al. provide solid evidence suggesting that G2 and S phases expressed protein 1 (GTSE1), is a previously unappreciated non-pocket substrate of cyclin D1-CDK4/6 kinases. To this end, this study holds a promise to significantly contribute to an improved understanding of the mechanisms underpinning cell cycle progression. Notwithstanding these clear strengths of the article, it was thought that the study may benefit from establishing the precise role of cyclin D1-CDK4/6 kinase-dependent GTSE1 phosphorylation in the context of cell cycle progression, obtaining more direct evidence that cyclin D1-CDK4/6 kinase phosphorylate indicated sites on GTSE1 (e.g., S454) and mapping a degron in GTSE1 whose function may be blocked by cyclin D1-CDK4/6 kinase-dependent phosphorylation.

Reviewed by eLife

Dimer-monomer transition defines a hyper-thermostable peptidoglycan hydrolase mined from bacterial proteome by lysin-derived antimicrobial peptide-primed screening

1. Li Zhang
2. Fen Hu
3. Zirong Zhao
4. Xinfeng Li
5. Mingyue Zhong
6. Jiajun He
7. Fangfang Yao
8. Xiaomei Zhang
9. Yuxuan Mao
10. Hongping Wei
11. Jin He
12. Hang Yang

• Curated by eLife

  eLife assessment

  This valuable study explores a new strategy of lysin-derived antimicrobial peptide-primed screening to find peptidoglycan hydrolases from bacterial proteomes. Using this strategy, the authors identified five peptidoglycan hydrolases from Acinetobacter baumannii, which they tested on various Gram-positive and Gram-negative pathogens for antimicrobial activity. The revised manuscript addressed most of the prior concerns, and the data presented are solid and will be of interest to microbiologists.

Reviewed by eLife

Disordered proteins interact with the chemical environment to tune their protective function during drying

1. Shraddha KC
2. Kenny Nguyen
3. Vincent Nicholson
4. Annie Walgren
5. Tony Trent
6. Edith Gollub
7. Sofia Romero
8. Alex S Holehouse
9. Shahar Sukenik
10. Thomas C Boothby

• Curated by eLife

  eLife assessment

  This important study investigates the sensitivity to endogenous cosolvents of three families of intrinsically disordered proteins involved with desiccation. The findings, drawn from well-designed experiments and calculations, suggest a functional synergy between sensitivity to small molecule solutes and convergent desiccation protection strategy. The evidence is found to be convincing, and the authors provide appropriate caveats since the study's conclusions are based on a small number of proteins. This work will be of interest to biochemists and biophysicists interested in the conformation-function relationship of intrinsically disordered proteins.

Reviewed by eLife

Early steps of protein disaggregation by Hsp70 chaperone and class B J-domain proteins are shaped by Hsp110

1. Wiktoria Sztangierska
2. Hubert Wyszkowski
3. Maria Pokornowska
4. Klaudia Kochanowicz
5. Michal Rychłowski
6. Krzysztof Liberek
7. Agnieszka Kłosowska

• Curated by eLife

  eLife assessment

  This study provides an important insight into the mechanisms of cooperation between Hsp70 and its cochaperones during reactivation of aggregated proteins. Based on convincing evidence, the authors demonstrate that the co-chaperone Hsp110 boosts disaggregation activity by enhancing Hsp70 recruitment to protein aggregates. This work is of broad interest to biochemists and cell biologists working in the protein homeostasis field.

Reviewed by eLife

Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses

1. Aya Matsui
2. Cathy J Spangler
3. Johannes Elferich
4. Momoko Shiozaki
5. Nikki Jean
6. Xiaowei Zhao
7. Maozhen Qin
8. Haining Zhong
9. Zhiheng Yu
10. Eric Gouaux

• Curated by eLife

  eLife assessment

  This fundamental study demonstrates a novel method for imaging glutamate receptors in situ via cryo-ET. The use of cutting-edge methods is well-described and is compelling. This paper is broadly relevant to biophysicists and neuroscientists.

Reviewed by eLife

DTX3L ubiquitin ligase ubiquitinates single-stranded nucleic acids

1. Emily L Dearlove
2. Chatrin Chatrin
3. Lori Buetow
4. Syed F Ahmed
5. Tobias Schmidt
6. Martin Bushell
7. Brian O Smith
8. Danny T Huang

• Curated by eLife

  eLife assessment

  This important study reports the discovery of a novel nucleotide ubiquitylation activity by the DTX3L E3 ligase. Solid evidence is presented for ubiquitin attachment to single-stranded oligonucleotides. This very interesting biochemical finding can be used as a starting point for studies to establish relevance in a physiological setting.

Reviewed by eLife

Evolutionarily divergent Mycobacterium tuberculosis CTP synthase filaments are under selective pressure

1. Eric M. Lynch
2. Yao Lu
3. Jin Ho Park
4. Lin Shao
5. Justin Kollman
6. E. Hesper Rego

Reviewed by PREreview

SIRT2-Mediated ACSS2 K271 Deacetylation Suppresses Lipogenesis Under Nutrient Stress

1. Rezwana Karim
2. Wendi Teng
3. Cameron Behram
4. Hening Lin

• Curated by eLife

  eLife assessment

  This useful study describes a role for acetylation in controlling the stability of acetyl-CoA synthetase 2, which converts acetate to acetyl-CoA for de novo lipid synthesis. While many aspects of the study are solid, some evidence supporting these findings is incomplete. Including direct demonstration of target deacetylation by sirtuin 2, revisiting statistical analyses, and confirming generalizability to adipocyte cell lines would further strengthen the study. This work will be of interest to researchers studying lipid metabolism and related diseases.

Reviewed by eLife

Structure of the Calvin-Benson-Bassham sedoheptulose-1,7-bisphosphatase from the model microalga Chlamydomonas reinhardtii

1. Théo Le Moigne
2. Martina Santoni
3. Lucile Jomat
4. Stéphane D Lemaire
5. Mirko Zaffagnini
6. Nicolas Chéron
7. Julien Henri

• Curated by eLife

  eLife assessment

  The manuscript reports valuable findings by resolving the crystal structure of Sedoheptulose-1,7-Bisphosphatase (SBPase) from the green algae Chlamydomonas reinhardtii, which is involved in the Calvin cycle. The data presented are solid, based on validated methodologies, and they help in understanding the structure and function of this enzyme.

Reviewed by eLife

Control of 3′ splice site selection by the yeast splicing factor Fyv6

1. Katherine Senn
2. Karli Lipinski
3. Natalie J. Zeps
4. Amory F. Griffin
5. Max E. Wilkinson
6. Aaron Hoskins

• Curated by eLife

  eLife assessment

  This important study addresses how 3' splice site choice is modulated by the conserved spliceosome-associated protein Fyv6. The authors provide compelling evidence Fyv6 functions to enable selection of 3' splice sites distal to a branch point and in doing so antagonizes more proximal, suboptimal 3' splice sites. The study would be improved through a more nuanced discussion of alternative possibilities and models, for instance in discussing the phenotypic impact of Fyv6 deletion.

Reviewed by eLife