Biochemistry

UV irradiation alters TFAM binding to mitochondrial DNA

1. Dillon E King
2. Emily E Beard
3. Matthew J Satusky
4. Ian Ryde
5. Alex George
6. Caitlin Johnson
7. Emma L Dolan
8. Yuning Zhang
9. Wei Zhu
10. Hunter Wilkins
11. Evan Corden
12. Susan K Murphy
13. Dorothy Erie
14. Raluca Gordân
15. Joel N Meyer

• Curated by eLife

  eLife Assessment

  Mitochondrial DNA (mtDNA) exhibits a degree of resistance to mutagenesis under genotoxic stress, and this study on the mitochondrial Transcription Factor A (TFAM) presents valuable data concerning the possible mechanisms involved. The presented data are solid, technically rigorous, and consistent with established literature findings. The experiments are well-executed, providing reliable evidence on the change of TFAM-DNA interactions following UVC irradiation. However, the evidence is inadequate to support the primary claims.

Reviewed by eLife

A high-resolution analysis of arrestin2 interactions responsible for CCR5 endocytosis

1. Ivana Petrovic
2. Samit Desai
3. Polina Isaikina
4. Layara Akemi Abiko
5. Anne Spang
6. Stephan Grzesiek

• Curated by eLife

  eLife Assessment

  The authors investigate arrestin2-mediated CCR5 endocytosis in the context of clathrin and AP2 contributions. Using an extensive set of NMR experiments, and supported by microscopy and other biophysical assays, the authors provide compelling data on the roles of AP2 and clathrin in CCR5 endocytosis. This important work will appeal to an audience beyond those studying chemokine receptors, including those studying GPCR regulation and trafficking. The distinct role of AP2 and not clathrin will be of particular interest to those studying GPCR internalization mechanisms.

Reviewed by eLife

Aggregation-Dependent Epitope Sequence and Modification Fingerprints of Anti-Aβ Antibodies

1. Ivan Talucci
2. Timon Leske
3. Hans-Wolfgang Klafki
4. Mohammed Mehedi Hassan
5. Annik Steiert
6. Barbara Morgado
7. Sebastian Bothe
8. Lars van Werven
9. Thomas Liepold
10. Jochen Walter
11. Hermann Schindelin
12. Jens Wiltfang
13. Oliver Wirths
14. Olaf Jahn
15. Hans Michael Maric

• Curated by eLife

  eLife Assessment

  Antibodies that selectively bind distinct amyloid-beta variants are vital tools for Alzheimer's disease research. This valuable manuscript aims to delineate the epitope specificity in a panel of anti-amyloid-beta antibodies, including some with clinical relevance. The experiments were rigorously conducted, employing an interesting combination of established and state-of-the-art methodologies, yielding convincing findings.

Reviewed by eLife

Investigation of All Disease-Relevant Lysine Acetylation Sites in α-Synuclein Enabled by Non-canonical Amino Acid Mutagenesis

1. Marie Shimogawa
2. Ming-Hao Li
3. Grace Shin Hye Park
4. Jennifer Ramirez
5. Hudson Lee
6. Paris R Watson
7. Swati Sharma
8. Zongtao Lin
9. Chao Peng
10. Virginia M.-Y Lee
11. Benjamin A Garcia
12. David W Christianson
13. Elizabeth Rhoades
14. David Eliezer
15. E James Petersson

• Curated by eLife

  eLife Assessment

  This valuable work provides new insights into the role of lysine acetylation of alpha-synuclein, the protein involved in Parkinson's Disease. The evidence is mostly solid, but the claims around the potential disease relevance based on seeding assays and structural work need to be toned down, or else supported by additional experimental evidence. Overall, the work will be of interest to researchers in the fields of protein biophysics and post-translational modifications, as well as Parkinson's Disease.

Reviewed by eLife

Profiling of terminating ribosomes reveals translational control at stop codons

1. Longfei Jia
2. Yuanhui Mao
3. Saori Uematsu
4. Xinyi Ashley Liu
5. Leiming Dong
6. Leonardo Henrique França de Lima
7. Shu-Bing Qian

• Curated by eLife

  eLife Assessment

  This manuscript reports on the application of ribosome profiling (EZRA-seq and eRF1-seq) combined with massively parallel reporter assays to identify and characterize a GA-rich element associated with ribosome pausing during translation termination. While the development of eRF1-seq is useful and the identification of GA-rich elements upstream of stop codons is convincing, the level of support for other claims is inadequate. Specifically, the evidence that GA-rich sequences upstream of stop codons can base-pair with the 3′ end of 18S rRNA to prolong ribosome dwell time, and the evidence that Rps26 interferes with this interaction to regulate translation termination, are not adequate.

Reviewed by eLife

Inhibition of NF-κB Signaling by the Reactive Glycolytic Metabolite Methylglyoxal

1. Caroline Stanton
2. Woojin Choi
3. R. Luke Wiseman
4. Michael J. Bollong

Reviewed by preLights

The Malate–Aspartate Shuttle supports thermogenic lipid mobilization in brown adipocytes

1. Michaela Veliova
2. Caroline M. Ferreira
3. Katrina Montales
4. Francisco Villalobos
5. Alexandra J. Brownstein
6. Rebeca Acín-Pérez
7. Gabrielly S. Ferreira
8. Linsey Stiles
9. Marc Liesa
10. Orian S. Shirihai
11. Marcus F. Oliveira

Reviewed by Review Commons

NanoBlocks: creating fluorescent biosensors from affinity binders using competitive binding

1. Silke Denis
2. Vincent Van Deuren
3. Desiree I. Frecot
4. Ulrich Rothbauer
5. Shehab Ismail
6. Alison G. Tebo
7. Peter Dedecker

Reviewed by Arcadia Science

Intrinsic specificity of a 'core' Tip60 acetyltransferase complex in Drosophila

1. Silke Krause
2. Marco Borsò
3. Marisa Müller
4. Axel Imhof
5. Zivkos Apostolou
6. Peter B. Becker

Reviewed by Review Commons

Computational design of pH-sensitive binders

1. Green Ahn
2. Brian Coventry
3. Ella Haefner
4. Shayan Sadre
5. Jenny Hu
6. Mimosa Van
7. Buwei Huang
8. Isaac Sappington
9. Adam J. Broerman
10. Mauriz A. Lichtenstein
11. Matthias Glögl
12. Inna Goreshnik
13. Dionne Vafeados
14. David Baker

Reviewed by preLights