Biochemistry

The Malate–Aspartate Shuttle supports thermogenic lipid mobilization in brown adipocytes

1. Michaela Veliova
2. Caroline M. Ferreira
3. Katrina Montales
4. Francisco Villalobos
5. Alexandra J. Brownstein
6. Rebeca Acín-Pérez
7. Gabrielly S. Ferreira
8. Linsey Stiles
9. Marc Liesa
10. Orian S. Shirihai
11. Marcus F. Oliveira

Reviewed by Review Commons

NanoBlocks: creating fluorescent biosensors from affinity binders using competitive binding

1. Silke Denis
2. Vincent Van Deuren
3. Desiree I. Frecot
4. Ulrich Rothbauer
5. Shehab Ismail
6. Alison G. Tebo
7. Peter Dedecker

Reviewed by Arcadia Science

Intrinsic specificity of a 'core' Tip60 acetyltransferase complex in Drosophila

1. Silke Krause
2. Marco Borsò
3. Marisa Müller
4. Axel Imhof
5. Zivkos Apostolou
6. Peter B. Becker

Reviewed by Review Commons

Computational design of pH-sensitive binders

1. Green Ahn
2. Brian Coventry
3. Ella Haefner
4. Shayan Sadre
5. Jenny Hu
6. Mimosa Van
7. Buwei Huang
8. Isaac Sappington
9. Adam J. Broerman
10. Mauriz A. Lichtenstein
11. Matthias Glögl
12. Inna Goreshnik
13. Dionne Vafeados
14. David Baker

Reviewed by preLights

Integrated Respirometry and Metabolomics Unveil Circadian Metabolic Dynamics in Drosophila

1. Farheen Akhtar
2. Dania Malik
3. Arjun Sengupta
4. Paula Haynes
5. Jaco Klok
6. Amita Sehgal
7. Aalim M Weljie

• Curated by eLife

  eLife Assessment

  This study investigates how sleep loss and circadian disruption affect whole-organ metabolism in flies (Drosophila melanogaster) and reports that wild-type flies align metabolism in anticipation of diurnal rhythm, while mutant flies with impaired sleep or circadian function shift to reactive or misaligned metabolism. The integration of chamber-based flow-through respirometry with LC-MS metabolomics is innovative, and the significance of the findings is valuable. However, the strength of evidence needed to support the conclusions is incomplete based on concerns regarding the inappropriate use of constant darkness to disrupt circadian rhythms and the lack of details justifying the methods used to correlate respirometry data with whole-body metabolomics.

Reviewed by eLife

Identification of the Regulatory Elements and Protein Substrates of Lysine Acetoacetylation

1. Qianyun Fu
2. Terry Nguyen
3. Bhoj Kumar
4. Parastoo Azadi
5. Y George Zheng

• Curated by eLife

  eLife Assessment

  This useful study reports a method to detect and analyze a novel post-translational modification, lysine acetoacetylation (Kacac), finding it regulates protein metabolism pathways. The study unveils epigenetic modifiers involved in placing this mark, including key histone acetyltransferases such as p300, and concomitant HDACs, which remove the mark. Proteomic and bioinformatics analysis identified many human proteins with Kacac sites, potentially suggesting broad effects on cellular processes and disease mechanisms. The data presented are solid, although some concerns persist regarding inconsistencies in molecular weight of the enzyme used. The study will be of interest to those studying protein and metabolic regulation.

Reviewed by eLife

Structure-based Design of Chimeric Influenza Hemagglutinins to Elicit Cross-group Immunity

1. Karla M Castro
2. Reyhaneh Ayardulabi
3. Sarah Wehrle
4. Hongrui Cui
5. Sandrine Georgeon
6. Joseph Schmidt
7. Shuhao Xiao
8. Nishat Seraj
9. Wayne Harshbarger
10. Corey P Mallett
11. Ventzislav Vassilev
12. Xavier Saelens
13. Bruno E Correia

• Curated by eLife

  eLife Assessment

  This important study presents a thoughtful design and characterization of chimeric influenza hemagglutinin (HA) head domains combining elements of distinct receptor-binding sites. The results provide convincing evidence that polyclonal cross-group responses to influenza A virus can be elicited by a single immunization. While the mechanistic basis of heterotrimer formation and immunodominance differences remains unclear, the authors provide new insights for protein design, vaccinology, and computational vaccine design.

Reviewed by eLife

Automated genome mining predicts structural diversity and taxonomic distribution of peptide metallophores across bacteria

1. Zachary L Reitz
2. Bita Pourmohsenin
3. Melanie Susman
4. Emil Thomsen
5. Daniel Roth
6. Alison Butler
7. Nadine Ziemert
8. Marnix H Medema

• Curated by eLife

  eLife Assessment

  This important and compelling study establishes a robust computational and experimental framework for the large-scale identification of metallophore biosynthetic clusters. The work advances beyond current standards, providing theoretical and practical value across microbiology, bioinformatics, and evolutionary biology.

Reviewed by eLife

Structures of Multiple Peptide Resistance Factor from Pseudomonas aeruginosa

1. Shaileshanand Jha
2. Kutti R. Vinothkumar

Reviewed by Review Commons

Membrane-mimetic thermal proteome profiling (MM-TPP) toward mapping membrane protein–ligand dynamic interactions

1. Rupinder Singh Jandu
2. Ashim Bhattacharya
3. Frank Antony
4. Mohammed Al-Seragi
5. Hiroyuki Aoki
6. Mohan Babu
7. Franck Duong van Hoa

• Curated by eLife

  eLife Assessment

  This valuable study introduces the peptidisc-TPP approach as a promising solution to challenges in membrane proteomics, enabling thermal proteome profiling in a detergent-free system. The concept is innovative and holds significant potential, and the demonstration of its utility and validation is solid. The method presents a strong foundation for broader applications in identifying physiologically and pharmacologically relevant membrane protein-ligand interactions.

Reviewed by eLife