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  • Diffusive lensing as a mechanism of intracellular transport and compartmentalization

    1. Achuthan Raja Venkatesh
    2. Kathy H. Le
    3. David M. Weld
    4. Onn Brandman
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    With diffusion being inversely correlated with viscosity, this super interesting modeling study got me thinking about intracellular transport based on viscosity gradients (viscophoresis) or non-homogeneous viscosity within a cell. They call this “diffusive lensing” and speculate it drives a lot of biological processes at the meso scale.

  • Functionally conserved Pkd2, mutated in autosomal dominant polycystic kidney disease, localizes to the endoplasmic reticulum and regulates cytoplasmic calcium homeostasis in fission yeast

    1. Takayuki Koyano
    2. Kazunori Kume
    3. Kaori Onishi
    4. Makoto Matsuyama
    5. Masaki Fukushima
    6. Takashi Toda
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    Pkd2 mutations result in autosomal dominant polycystic kidney disease (PKD), characterized as a ciliopathy due to the ciliary localization and impact of protein dysfunction, though the relationship with cilium structure and function for PKD remains murky given cilium disruption on a pkd2 mutant background can ameliorate cystic phenotypes. Studying functions of this protein OUTSIDE cilia via a model that lacks cilia entirely (yeast) is sure to be informative in teasing apart these complex interdependencies

  • Comparative analysis of actin visualization by genetically encoded probes in cultured neurons

    1. Attila Ignácz
    2. Domonkos Nagy-Herczeg
    3. Angelika Hausser
    4. Katalin Schlett
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    There are few things I enjoy more than a comparative analysis of actin probes. Another of my all time favorites is this:

  • A role for the centrosome in regulating the rate of neuronal efferocytosis by microglia in vivo

    1. Katrin Möller
    2. Max Brambach
    3. Ambra Villani
    4. Elisa Gallo
    5. Darren Gilmour
    6. Francesca Peri
    This article has 4 evaluationsAppears in 3 listsLatest version

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    This is an unbelievably interesting study on mictotubule and MTOC behavior during phagocytosis of apoptotic cells by microglia during zebrafish development. Check out the relative contributions of actin and mictotubules in branch mediated engulfment in figures 2E and F and the role of centrosome movement into these branches in figures 4 and 5 for successful phagocytosis, which can explain the one at a time behavior typically seen in these cells. This is tested further by artificially increasing centrosome number by centrin 4 over-expression and concurrent phagocytic events. They even look at vesicular trafficking and mechanisms of centrosome reorientation. Honestly this feels like several papers worth of work.

  • ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning

    1. Florian Fäßler
    2. Manjunath G Javoor
    3. Julia Datler
    4. Hermann Döring
    5. Florian W Hofer
    6. Georgi Dimchev
    7. Victor-Valentin Hodirnau
    8. Klemens Rottner
    9. Florian KM Schur
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    Understanding ARPC5 and its isoforms, a component of the branched actin nucleating Arp2/3 complex is a fascinating topic. They find here (in addition to effects on ARPC1 and ENA/VASP) that knocking out both isoforms eliminates lamellipodia (the branched actin sheets at the leading edge of migrating cells). Some fun facts: Chlamydomonas cells don’t seem to have an ARPC5 (though we are working to confirm this), nor lamallipodia AND ARPC5 is the target of a microRNA miR-133a that is up-regulated in many human cancers. There are lots of interesting migration/metastasis related implications for ARPC5 regulation.

  • Beyond sequence similarity: cross-phyla protein annotation by structural prediction and alignment

    1. Fabian Ruperti
    2. Nikolaos Papadopoulos
    3. Jacob Musser
    4. Milot Mirdita
    5. Martin Steinegger
    6. Detlev Arendt
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    If you’ve ever worked in a model or non-model system, finding putative orthologs can be difficult given low sequence homology. Structure is much more well conserved across taxa and given recent advancements in structural prediction made possible by AlphaFold2 and methods built upon it (here ColabFold), structural homology based functional annotation of proteins across phyla is easier than ever!

  • Condensate functionalization with motors directs their nucleation in space and allows manipulating RNA localization

    1. Audrey Cochard
    2. Adham Safieddine
    3. Pauline Combe
    4. Marie-Noëlle Benassy
    5. Dominique Weil
    6. Zoher Gueroui
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    A useful tool that can be used for testing functions of localized mRNAs and consequences of disrupting their targeting. Condensate scaffolds and chemically inducible condensates are engineered to test effects of motor proteins on mRNA/RNP localization. The scaffolds move based on typical motor direction (minus end microtubule motors were much faster for transport of non-functionalized condensate scaffolds, possibly due to availability of scaffolds and molecular crowding in different parts of the cell). Chemically inducible condensate formation on motors was achieved and mRNA recruitment to condensates successfully disrupted endogenous localization.

  • APC couples neuronal mRNAs to multiple kinesins, EB1 and shrinking microtubule ends for bidirectional mRNA motility

    1. Sebastian J. Baumann
    2. Julia Grawenhoff
    3. Elsa C. Rodrigues
    4. Silvia Speroni
    5. Maria Gili
    6. Artem Komissarov
    7. Sebastian P. Maurer
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    I somehow missed this the first time but this [revision] is unbelievably cool. APC can bind the 3’UTR of RNAs as an adaptor for kinesin-1 and -2 (the ciliary kinesin!) for trafficking on mictotubules. This is shown via in vitro reconstitution experiments and it appears that these complexes track plus ends in an EB1 dependent manner but can stay associated in shrinking MTs in an EB1-independent manner.

  • Multifunctional fluorophores for live-cell imaging and affinity capture of proteins

    1. Pratik Kumar
    2. Jason D. Vevea
    3. Edwin R. Chapman
    4. Luke D. Lavis
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    This is awesome. Multifunctional cell permeable ligands (fluorophore+biotin) that can be used with haloTag fusion proteins for both visualization and affinity capture!

  • Large-scale identification of phospho-modulated motif-based protein-protein interactions

    1. Johanna Kliche
    2. Dimitriya Hristoforova Garvanska
    3. Leandro Simonetti
    4. Dilip Badgujar
    5. Doreen Dobritzsch
    6. Jakob Nilsson
    7. Norman Davey
    8. Ylva Ivarsson
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    Omg this is so cool! A peptide phage display approach to screen for functionally relevant phospho-sites. They do some structural, evolutionary and other analyses to prioritize phosphorites within intrinsically disordered regions containing short linear motifs that often mediate phospho-dependent interactions. They then screen through expression/binding to uncover novel interactions mediated by phosphorylation that they are able to validate in many instances. Neat approach and a wealth of data to be mined. Check out the supplemental tables.

  • Granger-causal inference of the lamellipodial actin regulator hierarchy by live cell imaging without perturbation

    1. Jungsik Noh
    2. Tadamoto Isogai
    3. Joseph Chi
    4. Kushal Bhatt
    5. Gaudenz Danuser
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    Super interesting study on inferring causal relationships between molecular pathways and cell behavior, something I’m very interested in given molecular detail isn’t accessible for so many cells/species.

  • Targeted protein degradation using degradFP in Trypanosoma brucei

    1. Midori Ishii
    2. Bungo Akiyoshi
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    Nice! Targeted degradation of GFP tagged proteins in Trypanosomes. They applied degradFP, which fuses a GFP nanobody with a protein that recruits an E3 ubiquitin ligase, causing ubiquitination and degradation of the GFP-tagged target.

  • TTBK2 controls cilium stability through actin and the centrosomal compartment

    1. Abraham Nguyen
    2. Sarah C. Goetz
    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity

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    Some interesting interactions here consistent with actin’s role in restricting mammalian ciliogenesis: decreased ciliation and increased breakage caused by inducible disruption of Tau Tubulin Kinase 2 is partially rescued by inhibitors of actin polymerization and myosin VI.

  • Cell walls are dynamically O-acetylated in the green seaweed, Ulva compressa

    1. John H. Bothwell
    2. Alexander J. Goodridge
    3. Marie Rapin
    4. Patrick J. Brennan
    5. Alexandra Traslaviña López
    6. Akanksha Agrawal
    7. Stephen C. Fry
    8. Georgia Campbell
    9. Jonas Blomme
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    An interesting paper identifying stress-induced modification (O-acetylation) in the cell wall of the green seaweed, Ulva compressa. It contains a protocol for chlorophyte cell wall fractionation.

  • Signal Peptide Efficiency: from High-throughput Data to Prediction and Explanation

    1. Stefano Grasso
    2. Valentina Dabene
    3. Margriet M.W.B. Hendriks
    4. Priscilla Zwartjens
    5. René Pellaux
    6. Martin Held
    7. Sven Panke
    8. Jan Maarten van Dijl
    9. Andreas Meyer
    10. Tjeerd van Rij
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    Whoa nice, a systematic analyses of thousands of signal peptide sequences and effects on properties affecting secretion

  • ERK pathway activation inhibits ciliogenesis and causes defects in motor behavior, ciliary gating, and cytoskeletal rearrangement

    1. Larissa L Dougherty
    2. Soumita Dutta
    3. Prachee Avasthi
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    Again I get to highlight one of our new preprints! This one tackles the intricate interplay between cell division and ciliogenesis. Using genetics, biochemistry, four different imaging modalities, and two organisms, we identified cytoskeletal, motor, and trafficking defects causing impaired cilium formation when activating a major cell proliferation and cancer pathway.

  • Blind spots on western blots: a meta-research study highlighting opportunities to improve figures and methods reporting

    1. Cristina Kroon
    2. Larissa Breuer
    3. Lydia Jones
    4. Jeehye An
    5. Ayça Akan
    6. Elkhansa Ahmed Mohamed Ali
    7. Felix Busch
    8. Marinus Fislage
    9. Biswajit Ghosh
    10. Max Hellrigel-Holderbaum
    11. Vartan Kazezian
    12. Alina Koppold
    13. Cesar Alberto Moreira Restrepo
    14. Nico Riedel
    15. Lea Scherschinski
    16. Fernando Raúl Urrutia Gonzalez
    17. Tracey Weissgerber
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    There may not be a technique as over-interpreted relative to the information that it can provide as a western blot but this is made worse by ignoring best practices and my favorite, using a single protein as an unvalidated loading control. This analysis of westerns from the literature highlights some better display and reporting practices