@AvasthiReading's saved articles
A list by Prachee Avasthi Recommended Reading
See what researchers at Prachee Avasthi’s lab are reading to discover some interesting new work.
Showing page 2 of 10 pages of list content
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Blind spots on western blots: a meta-research study highlighting opportunities to improve figures and methods reporting
This article's authorsAnnotation by AvasthiReading
There may not be a technique as over-interpreted relative to the information that it can provide as a western blot but this is made worse by ignoring best practices and my favorite, using a single protein as an unvalidated loading control. This analysis of westerns from the literature highlights some better display and reporting practices
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Vast heterogeneity in cytoplasmic diffusion rates revealed by nanorheology and Doppelgänger simulations
This article's authorsAnnotation by AvasthiReading
Stunning result of immense heterogeneity possible in cytoplasmic viscosity affecting diffusive cellular processes
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Liquid-like VASP condensates drive actin polymerization and dynamic bundling
This article's authorsAnnotation by AvasthiReading
I have no idea whether this actually happens in a cell but what an interesting model! I think I audibly gasped at figure 3A.
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Ultrastructure Expansion Microscopy reveals the nanoscale cellular architecture of budding and fission yeast
This article's authorsAnnotation by AvasthiReading
Spectacular expansion microscopy in yeast! I went straight for the cell wall digestion protocol so we can try it in a number of different algae
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Condensation of the fusion focus by the intrinsically disordered region of the formin Fus1 is essential for cell-cell fusion
This article's authorsAnnotation by AvasthiReading
This is so cool! The intrinsically disordered region in a pombe formin is needed to generate the foci needed for actin mediated cell fusion. Both replacement and rescue generate the expected behaviors associated with condensation state. Lots of interesting papers recently on condensates mediating behaviors of actin regulators and polymerization!
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Protrusion growth driven by myosin-generated force
This article's authorsAnnotation by AvasthiReading
Interesting study showing that membrane-anchored myosin can generate the force necessary for membrane protrusion beyond actin polymerization based mechanisms
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Centriole growth is not limited by a finite pool of components, but is limited by the Cdk1/Cyclin-dependent phosphorylation of Ana2/STIL
This article's authorsAnnotation by AvasthiReading
Limiting component models in organelle size control are difficult to pin down as the limiting components themselves are hard to identify. This study identifies an alternate kinase-dependent model for centriole size control.
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Ultrasensitivity of Microtubule severing rate on the concentration of free Tubulin
This article's authorsAnnotation by AvasthiReading
Nice! I haven’t played with this yet but it’s a new image analysis web app, Image Data Explorer.
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Lithium-induced ciliary lengthening sparks Arp2/3 complex-dependent endocytosis
This article's authorsAnnotation by AvasthiReading
This week, I get to highlight our own preprint proposing a putative mechanism of lithium-mediated cilium extension through stimulating endocytosis to supply membrane to the cilium from the plasma membrane. We proposed this rapid route for ciliary material in our last preprint
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Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Fornicata
This article's authorsAnnotation by AvasthiReading
Incredibly interesting study of endocytic adaptation in an obligate parasite in which a structural analog of clathrin light chain is predicted/identified.
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Kinesin-1 transports morphologically distinct intracellular virions during vaccinia infection
This article's authorsAnnotation by AvasthiReading
Do yourself a favor and check out this exquisite characterization of vaccinia viron transport towards microtubule plus ends. In-cell deletion and microscopy, in vitro reconstitution, quantification of motor complexes associated with virons and viron speed … really beautiful work!
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A quantitative and spatial analysis of cell cycle regulators during the fission yeast cycle
This article's authorsAnnotation by AvasthiReading
I love resources like this. A comprehensive look at expression levels and localization data for all 38 mitotic regulators in fission yeast during division.
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Reconstructing physical cell interaction networks from single-cell data using Neighbor-seq
This article's authorsAnnotation by AvasthiReading
Huh clever. Leveraging incomplete dissociation in scRNA-seq experiments to infer tissue architecture/cell-cell interactions.
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Conserved degronome features governing quality control associated proteolysis
This article's authorsAnnotation by AvasthiReading
How interesting! A peptide screen to identify degrons with E3 ubiquitin ligase specificity in yeast followed by extraction of conserved properties identifies transmembrane domain containing peptides as likely degrons.
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Integrating bulk and single cell RNA-seq refines transcriptomic profiles of specific C. elegans neurons
This article's authorsAnnotation by AvasthiReading
Recognizing the utility of not using exclusively cutting edge methods for everything, this important study combines bulk RNAseq (high sensitivity due to capturing more low expression and noncoding RNAs) and single cell RNAseq (high specificity) for a more comprehensive transcriptomic picture.
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Bio-hybrid micro-swimmers propelled by flagella isolated from C. reinhardtii †
This article's authorsAnnotation by AvasthiReading
This is wild! Chlamydomonas flagella were isolated, demembranated, attached to polystyrene beads and reactivated to reconstitute flagellar movement. This yields some interesting insights through experimentation and simulation: Increased calcium concentration affects flagellar waveform and trajectory of bead movement and surprisingly, larger beads have increased rather than decreased velocity.
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ARP2/3 complex associates with peroxisomes to participate in pexophagy in plants
This article's authorsAnnotation by AvasthiReading
Interesting role for ARP2/3 complex in peroxisome autophagy in plants!
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Rapid Rerouting of Myosin Traffic at the T Cell Immunological Synapse
This article's authorsAnnotation by AvasthiReading
This polarity of actin networks and myosin traffic is interesting in light of the parallels between cilium biogenesis and the immune synapse function.
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Structural basis of actin filament assembly and aging
This article's authorsAnnotation by AvasthiReading
A 2.2 angstrom resolution structures of muscle actin filaments in ATP, ADP-Pi and ADP states. Many new insights here about the surprising stability of ADP actin, mechanism of ATP hydrolysis, cofilin binding and more.
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Genome-wide functional analysis reveals key roles for kinesins in the mammalian and mosquito stages of the malaria parasite life cycle
This article's authorsAnnotation by AvasthiReading
Truly exquisite characterization of kinesins in the malaria parasite. Check out the spectacular expansion microscopy images in figure 7A!