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  • Liquid-like VASP condensates drive actin polymerization and dynamic bundling

    1. Kristin Graham
    2. Aravind Chandrasekaran
    3. Liping Wang
    4. Aly Ladak
    5. Eileen M. Lafer
    6. Padmini Rangamani
    7. Jeanne C. Stachowiak
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    I have no idea whether this actually happens in a cell but what an interesting model! I think I audibly gasped at figure 3A.

  • Ultrastructure Expansion Microscopy reveals the nanoscale cellular architecture of budding and fission yeast

    1. Kerstin Hinterndorfer
    2. Marine. H. Laporte
    3. Felix Mikus
    4. Lucas Tafur Petrozzi
    5. Clélia Bourgoint
    6. Manoel Prouteau
    7. Gautam Dey
    8. Robbie Loewith
    9. Paul Guichard
    10. Virginie Hamel
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    Spectacular expansion microscopy in yeast! I went straight for the cell wall digestion protocol so we can try it in a number of different algae

  • Condensation of the fusion focus by the intrinsically disordered region of the formin Fus1 is essential for cell-cell fusion

    1. Ingrid Billault-Chaumartin
    2. Olivia Muriel
    3. Laetitia Michon
    4. Sophie G Martin
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    This is so cool! The intrinsically disordered region in a pombe formin is needed to generate the foci needed for actin mediated cell fusion. Both replacement and rescue generate the expected behaviors associated with condensation state. Lots of interesting papers recently on condensates mediating behaviors of actin regulators and polymerization!

  • Protrusion growth driven by myosin-generated force

    1. Gillian N. Fitz
    2. Meredith L. Weck
    3. Caroline Bodnya
    4. Olivia L. Perkins
    5. Matthew J. Tyska
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    Interesting study showing that membrane-anchored myosin can generate the force necessary for membrane protrusion beyond actin polymerization based mechanisms

  • Centriole growth is not limited by a finite pool of components, but is limited by the Cdk1/Cyclin-dependent phosphorylation of Ana2/STIL

    1. Thomas L. Steinacker
    2. Siu-Shing Wong
    3. Zsofia A. Novak
    4. Saroj Saurya
    5. Lisa Gartenmann
    6. Eline J.H. van Houtum
    7. Judith R. Sayers
    8. B. Christoffer Lagerholm
    9. Jordan W. Raff
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    Limiting component models in organelle size control are difficult to pin down as the limiting components themselves are hard to identify. This study identifies an alternate kinase-dependent model for centriole size control.

  • Lithium-induced ciliary lengthening sparks Arp2/3 complex-dependent endocytosis

    1. Brae M Bigge
    2. Prachee Avasthi
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    This week, I get to highlight our own preprint proposing a putative mechanism of lithium-mediated cilium extension through stimulating endocytosis to supply membrane to the cilium from the plasma membrane. We proposed this rapid route for ciliary material in our last preprint

  • Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Fornicata

    1. Rui Santos
    2. Ásgeir Ástvaldsson
    3. Shweta V. Pipaliya
    4. Jon Paulin Zumthor
    5. Joel B. Dacks
    6. Staffan Svärd
    7. Adrian B. Hehl
    8. Carmen Faso
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    Incredibly interesting study of endocytic adaptation in an obligate parasite in which a structural analog of clathrin light chain is predicted/identified.

  • Kinesin-1 transports morphologically distinct intracellular virions during vaccinia infection

    1. Amadeus Xu
    2. Angika Basant
    3. Sibylle Schleich
    4. Timothy P Newsome
    5. Michael Way
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    Do yourself a favor and check out this exquisite characterization of vaccinia viron transport towards microtubule plus ends. In-cell deletion and microscopy, in vitro reconstitution, quantification of motor complexes associated with virons and viron speed … really beautiful work!

  • Conserved degronome features governing quality control associated proteolysis

    1. Bayan Mashahreh
    2. Shir Armony
    3. Kristoffer Enøe Johansson
    4. Alon Chappleboim
    5. Nir Friedman
    6. Richard G. Gardner
    7. Rasmus Hartmann-Petersen
    8. Kresten Lindorff-Larsen
    9. Tommer Ravid
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    How interesting! A peptide screen to identify degrons with E3 ubiquitin ligase specificity in yeast followed by extraction of conserved properties identifies transmembrane domain containing peptides as likely degrons.

  • Integrating bulk and single cell RNA-seq refines transcriptomic profiles of specific C. elegans neurons

    1. Alec Barrett
    2. Erdem Varol
    3. Alexis Weinreb
    4. Seth R. Taylor
    5. Rebecca M. McWhirter
    6. Cyril Cros
    7. Manasa Basaravaju
    8. Abigail Poff
    9. John A. Tipps
    10. Maryam Majeed
    11. Berta Vidal
    12. Chen Wang
    13. Eviatar Yemini
    14. Emily A. Bayer
    15. HaoSheng Sun
    16. Oliver Hobert
    17. David M. Miller
    18. Marc Hammarlund
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    Recognizing the utility of not using exclusively cutting edge methods for everything, this important study combines bulk RNAseq (high sensitivity due to capturing more low expression and noncoding RNAs) and single cell RNAseq (high specificity) for a more comprehensive transcriptomic picture.

  • Bio-hybrid micro-swimmers propelled by flagella isolated from C. reinhardtii

    1. Raheel Ahmad
    2. Albert J Bae
    3. Yu-Jung Su
    4. Samira Goli Pozveh
    5. Eberhard Bodenschatz
    6. Alain Pumir
    7. Azam Gholami
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    This is wild! Chlamydomonas flagella were isolated, demembranated, attached to polystyrene beads and reactivated to reconstitute flagellar movement. This yields some interesting insights through experimentation and simulation: Increased calcium concentration affects flagellar waveform and trajectory of bead movement and surprisingly, larger beads have increased rather than decreased velocity.

  • ARP2/3 complex associates with peroxisomes to participate in pexophagy in plants

    1. Jan Martinek
    2. Petra Cifrová
    3. Stanislav Vosolsobě
    4. Jana Krtková
    5. Lenka Sikorová
    6. Kateřina Malínská
    7. Zdeňka Mauerová
    8. Ian Leaves
    9. Imogen Sparkes
    10. Kateřina Schwarzerová
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    Interesting role for ARP2/3 complex in peroxisome autophagy in plants!

  • Rapid Rerouting of Myosin Traffic at the T Cell Immunological Synapse

    1. Robert Mąka
    2. Natalia Plewa
    3. Urszula Cichoń
    4. Katarzyna Krysztofiak
    5. Jagoda J. Rokicka
    6. Ronald S. Rock
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    This polarity of actin networks and myosin traffic is interesting in light of the parallels between cilium biogenesis and the immune synapse function.

  • Structural basis of actin filament assembly and aging

    1. Wout Oosterheert
    2. Björn U Klink
    3. Alexander Belyy
    4. Sabrina Pospich
    5. Stefan Raunser
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    A 2.2 angstrom resolution structures of muscle actin filaments in ATP, ADP-Pi and ADP states. Many new insights here about the surprising stability of ADP actin, mechanism of ATP hydrolysis, cofilin binding and more.

  • Genome-wide functional analysis reveals key roles for kinesins in the mammalian and mosquito stages of the malaria parasite life cycle

    1. Mohammad Zeeshan
    2. Ravish Rashpa
    3. David J Ferguson
    4. Steven Abel
    5. Zeinab Chahine
    6. Declan Brady
    7. Sue Vaughan
    8. Carolyn A. Moores
    9. Karine G. Le Roch
    10. Mathieu Brochet
    11. Anthony A. Holder
    12. Rita Tewari
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    Truly exquisite characterization of kinesins in the malaria parasite. Check out the spectacular expansion microscopy images in figure 7A!

  • The molecular organization of flat and curved caveolae indicates bendable structural units at the plasma membrane

    1. Claudia Matthaeus
    2. Kem A. Sochacki
    3. Andrea Dickey
    4. Dmytro Puchkov
    5. Volker Haucke
    6. Martin Lehmann
    7. Justin W. Taraska
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    This is a beautiful characterization of caveolae. In addition to platinum replica EM of these plasma membrane invaginations in various cell types, this work includes molecular identification of proteins associated with caveolae of different curvatures by STED-CLEM (very high-resolution correlative light electron microscopy).

  • Early Endosome Capture Proteomics and its Application to Amyloid Precursor Protein Intramembrane Processing by β and γ-Secretases

    1. Hankum Park
    2. Qing Yu
    3. Frances V. Hundley
    4. Katherine Overmyer
    5. Lia Serrano
    6. Joao A. Paulo
    7. Julia C. Paoli
    8. Sharan Swarup
    9. Joshua J. Coon
    10. Steven P. Gygi
    11. J. Wade Harper
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    Whoa! Successful ID of early endosomal cargoes via pull-down of an early endosomal marker followed by proteomics.