@AvasthiReading's saved articles
A list by Prachee Avasthi Recommended Reading
See what researchers at Prachee Avasthi’s lab are reading to discover some interesting new work.
Showing page 2 of 10 pages of list content
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Granger-causal inference of the lamellipodial actin regulator hierarchy by live cell imaging without perturbation
This article has 5 authors:Prachee Avasthi Recommended Reading
Super interesting study on inferring causal relationships between molecular pathways and cell behavior, something I’m very interested in given molecular detail isn’t accessible for so many cells/species.
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Targeted protein degradation using deGradFP in Trypanosoma brucei
This article has 2 authors: -
TTBK2 controls cilium stability through actin and the centrosomal compartment
This article has 2 authors:Reviewed by Review Commons
Prachee Avasthi Recommended Reading
Some interesting interactions here consistent with actin’s role in restricting mammalian ciliogenesis: decreased ciliation and increased breakage caused by inducible disruption of Tau Tubulin Kinase 2 is partially rescued by inhibitors of actin polymerization and myosin VI.
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Improved Methods for Bulk Cultivation and Fixation of Loxodes Ciliates for Fluorescence Microscopy
This article has 4 authors:Prachee Avasthi Recommended Reading
Quite impressive morphology preservation and immunofluorescence results with a new fixative containing zinc sulfate, formaldehyde, acetic acid, & ethanol for Loxodes (a ciliate genus)
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Cell walls are dynamically O-acetylated in the green seaweed, Ulva compressa
This article has 9 authors:Prachee Avasthi Recommended Reading
An interesting paper identifying stress-induced modification (O-acetylation) in the cell wall of the green seaweed, Ulva compressa. It contains a protocol for chlorophyte cell wall fractionation.
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Signal Peptide Efficiency: From High-Throughput Data to Prediction and Explanation
This article has 10 authors:Prachee Avasthi Recommended Reading
Whoa nice, a systematic analyses of thousands of signal peptide sequences and effects on properties affecting secretion
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ERK pathway activation inhibits ciliogenesis and causes defects in motor behavior, ciliary gating, and cytoskeletal rearrangement
This article has 3 authors:Reviewed by Review Commons, ASAPbio crowd review
Prachee Avasthi Recommended Reading
Again I get to highlight one of our new preprints! This one tackles the intricate interplay between cell division and ciliogenesis. Using genetics, biochemistry, four different imaging modalities, and two organisms, we identified cytoskeletal, motor, and trafficking defects causing impaired cilium formation when activating a major cell proliferation and cancer pathway.
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Blind spots on western blots: Assessment of common problems in western blot figures and methods reporting with recommendations to improve them
This article has 17 authors:Prachee Avasthi Recommended Reading
There may not be a technique as over-interpreted relative to the information that it can provide as a western blot but this is made worse by ignoring best practices and my favorite, using a single protein as an unvalidated loading control. This analysis of westerns from the literature highlights some better display and reporting practices
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Vast heterogeneity in cytoplasmic diffusion rates revealed by nanorheology and Doppelgänger simulations
This article has 4 authors:Prachee Avasthi Recommended Reading
Stunning result of immense heterogeneity possible in cytoplasmic viscosity affecting diffusive cellular processes
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Liquid-like VASP condensates drive actin polymerization and dynamic bundling
This article has 7 authors:Prachee Avasthi Recommended Reading
I have no idea whether this actually happens in a cell but what an interesting model! I think I audibly gasped at figure 3A.
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Ultrastructure Expansion Microscopy reveals the nanoscale cellular architecture of budding and fission yeast
This article has 10 authors:Prachee Avasthi Recommended Reading
Spectacular expansion microscopy in yeast! I went straight for the cell wall digestion protocol so we can try it in a number of different algae
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Condensation of the fusion focus by the intrinsically disordered region of the formin Fus1 is essential for cell-cell fusion
This article has 4 authors:Prachee Avasthi Recommended Reading
This is so cool! The intrinsically disordered region in a pombe formin is needed to generate the foci needed for actin mediated cell fusion. Both replacement and rescue generate the expected behaviors associated with condensation state. Lots of interesting papers recently on condensates mediating behaviors of actin regulators and polymerization!
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Protrusion growth driven by myosin-generated force
This article has 5 authors:Prachee Avasthi Recommended Reading
Interesting study showing that membrane-anchored myosin can generate the force necessary for membrane protrusion beyond actin polymerization based mechanisms
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Centriole growth is limited by the Cdk/Cyclin-dependent phosphorylation of Ana2/STIL
This article has 9 authors:Reviewed by Review Commons
Prachee Avasthi Recommended Reading
Limiting component models in organelle size control are difficult to pin down as the limiting components themselves are hard to identify. This study identifies an alternate kinase-dependent model for centriole size control.
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Ultrasensitivity of Microtubule severing rate on the concentration of free Tubulin
This article has 3 authors:Prachee Avasthi Recommended Reading
Nice! I haven’t played with this yet but it’s a new image analysis web app, Image Data Explorer.
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Lithium-induced ciliary lengthening sparks Arp2/3 complex–dependent endocytosis
This article has 3 authors:Reviewed by Review Commons
Prachee Avasthi Recommended Reading
This week, I get to highlight our own preprint proposing a putative mechanism of lithium-mediated cilium extension through stimulating endocytosis to supply membrane to the cilium from the plasma membrane. We proposed this rapid route for ciliary material in our last preprint
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Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Fornicata
This article has 8 authors:Prachee Avasthi Recommended Reading
Incredibly interesting study of endocytic adaptation in an obligate parasite in which a structural analog of clathrin light chain is predicted/identified.
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Kinesin-1 transports morphologically distinct intracellular virions during vaccinia infection
This article has 5 authors:Prachee Avasthi Recommended Reading
Do yourself a favor and check out this exquisite characterization of vaccinia viron transport towards microtubule plus ends. In-cell deletion and microscopy, in vitro reconstitution, quantification of motor complexes associated with virons and viron speed … really beautiful work!
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A quantitative and spatial analysis of cell cycle regulators during the fission yeast cycle
This article has 4 authors:Prachee Avasthi Recommended Reading
I love resources like this. A comprehensive look at expression levels and localization data for all 38 mitotic regulators in fission yeast during division.
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Reconstructing physical cell interaction networks from single-cell data using Neighbor-seq
This article has 2 authors:Prachee Avasthi Recommended Reading
Huh clever. Leveraging incomplete dissociation in scRNA-seq experiments to infer tissue architecture/cell-cell interactions.