eLife

Latest preprint reviews

1. Dual targeting of salt inducible kinases and CSF1R uncouples bone formation and bone resorption

   This article has 26 authors:

   1. Cheng-Chia Tang
   2. Christian D Castro Andrade
   3. Maureen J O'Meara
   4. Sung-Hee Yoon
   5. Tadatoshi Sato
   6. Daniel J Brooks
   7. Mary L Bouxsein
   8. Janaina da Silva Martins
   9. Jinhua Wang
   10. Nathanael S Gray
   11. Barbara Misof
   12. Paul Roschger
   13. Stephane Blouin
   14. Klaus Klaushofer
   15. Annegreet Velduis-Vlug
   16. Yosta Vegting
   17. Clifford J Rosen
   18. Daniel O'Connell
   19. Thomas B Sundberg
   20. Ramnik J Xavier
   21. Peter Ung
   22. Avner Schlessinger
   23. Henry M Kronenberg
   24. Rebecca Berdeaux
   25. Marc Foretz
   26. Marc N Wein

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is a very interesting, novel and informative study. The effects of the inhibitor on CSF1R inhibition are convincing and provide a compelling explanation for the net effects of the compound on the skeleton. The study opens the way for another possible oral therapeutic for osteoporosis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

2. GSK3 inhibition rescues growth and telomere dysfunction in dyskeratosis congenita iPSC-derived type II alveolar epithelial cells

   This article has 18 authors:

   1. Rafael Jesus Fernandez
   2. Zachary JG Gardner
   3. Katherine J Slovik
   4. Derek C Liberti
   5. Katrina N Estep
   6. Wenli Yang
   7. Qijun Chen
   8. Garrett T Santini
   9. Javier V Perez
   10. Sarah Root
   11. Ranvir Bhatia
   12. John W Tobias
   13. Apoorva Babu
   14. Michael P Morley
   15. David B Frank
   16. Edward E Morrisey
   17. Christopher J Lengner
   18. F Brad Johnson

   This article has been curated by 1 group:

   • Curated by eLife

     Summary: The investigators' study of human AT2 cells-derived from induced pluripotent stem cells (iAT2 cells) in the presence and absence of a known dyskeratosis congenita (DC) pathogenic variant provides an exceptional model for understanding pathogenesis of pulmonary fibrosis in dyskeratosis congenita (DC) and related telomere biology disorders (TBDs). They provide convincing data demonstrating altered WNT signaling in iAT2 cells with short, dysfunctional telomeres and improved growth of iAT2 cells by GSK3 inhibition but fall short of convincingly showing the latter is due restored telomere end protection. The work should be of interest to those in the fields of telomere biology and the TBDs, lung physiology, WNT signaling and stem cell biology.

   Reviewed by eLife

3. Global epistasis emerges from a generic model of a complex trait

   This article has 2 authors:

   1. Gautam Reddy
   2. Michael M Desai

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors consider Darwinian evolution for large systems, with a main focus on how adaptation changes over time. Frequently observed patterns of declining adaptability for a population in a new environment are discussed, i.e., that fitness tends to increase fast initially and then at a slower rate. Another topic is historical contingency in adaptation. A condition for minimal contingency is provided, and a new model (the connectedness model, or CN model) is introduced accordingly. The manuscript is innovative, conceptually interesting, and provides quantitative precision beyond most related studies in the field. However, the presentation currently does not work well for a general audience.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewers #1, #2, and #3 agreed to share their names with the authors.)

   Reviewed by eLife

4. Competitive coordination of the dual roles of the Hedgehog co-receptor in homophilic adhesion and signal reception

   This article has 10 authors:

   1. Shu Yang
   2. Ya Zhang
   3. Chuxuan Yang
   4. Xuefeng Wu
   5. Sarah Maria El Oud
   6. Rongfang Chen
   7. Xudong Cai
   8. Xufeng S Wu
   9. Ganhui Lan
   10. Xiaoyan Zheng

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is a well-conceived and well-presented study that sheds light on two critical questions related to Hedgehog signaling, namely, the dual function of the Hedgehog co-receptor Ihog in Hh signal transduction and homophilic adhesion, and the regulation of cytoneme structure.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis

   This article has 15 authors:

   1. Jia Li
   2. Xuan Qin
   3. Jie Shi
   4. Xiaoshuang Wang
   5. Tong Li
   6. Mengyao Xu
   7. Xiaosu Chen
   8. Yujia Zhao
   9. Jiahao Han
   10. Yongjun Piao
   11. Wenwen Zhang
   12. Pengpeng Qu
   13. Longlong Wang
   14. Rong Xiang
   15. Yi Shi

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors studied ovarian carcinoma and identified a potential role of interleukin 20 receptor subunit alpha (IL20RA) and of IL-20 in regulating the transcoelomic metastasis of ovarian carcinoma, where IL-20 signaling in tumors is protective. This leads to the production of IL-18 and an M1 macrophage phenotype, with reduction of metastasis. The study is of interest to investigators in the area of cancer and cytokines and has potential therapeutic ramifications.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. Misic, a general deep learning-based method for the high-throughput cell segmentation of complex bacterial communities

   This article has 10 authors:

   1. Swapnesh Panigrahi
   2. Dorothée Murat
   3. Antoine Le Gall
   4. Eugénie Martineau
   5. Kelly Goldlust
   6. Jean-Bernard Fiche
   7. Sara Rombouts
   8. Marcelo Nöllmann
   9. Leon Espinosa
   10. Tâm Mignot

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this work, Panigrahi et. al. develop a powerful deep-learning-based cell segmentation platform (MiSiC) capable of accurately segmenting brightfield, fluorescence, and phase contrast images of bacteria cells densely packed within both homogenous and heterogeneous cell populations. This algorithm, if further optimized and disseminated to the community, will have a large impact in microbial studies in that it will allow for automated analyses of essential every aspect of bacterial cell biology, including cell morphology, cell cycle, cell-cell communications, protein localization dynamics and a variety of cellular processes using time-lapse imaging.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

7. Parallel evolution between genomic segments of seasonal human influenza viruses reveals RNA-RNA relationships

   This article has 6 authors:

   1. Jennifer E Jones
   2. Valerie Le Sage
   3. Gabriella H Padovani
   4. Michael Calderon
   5. Erik S Wright
   6. Seema S Lakdawala

   Reviewed by eLife

8. Expansion of CD10neg neutrophils and CD14+HLA-DRneg/low monocytes driving proinflammatory responses in patients with acute myocardial infarction

   This article has 6 authors:

   1. Daniela Fraccarollo
   2. Jonas Neuser
   3. Julian Möller
   4. Christian Riehle
   5. Paolo Galuppo
   6. Johann Bauersachs

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of broad interest to cardiologist and scientists studying acute myocardial infarction (AMI), especially to those focussing on the immune responses during AMI. Using combination of in vivo and in vitro model, as well as tissue from patients, the authors reveal new insights regarding the immune mechanisms during AMI, highlighting the importance of neutrophils and monocytes during the early days of its process. The findings in this paper add to the understanding of how immune mechanisms may contribute to subsequent adverse events after AMI.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors)

   Reviewed by eLife

9. T-cell receptor (TCR) signaling promotes the assembly of RanBP2/RanGAP1-SUMO1/Ubc9 nuclear pore subcomplex via PKC-θ-mediated phosphorylation of RanGAP1

   This article has 11 authors:

   1. Yujiao He
   2. Zhiguo Yang
   3. Chen-si Zhao
   4. Zhihui Xiao
   5. Yu Gong
   6. Yun-Yi Li
   7. Yiqi Chen
   8. Yunting Du
   9. Dianying Feng
   10. Amnon Altman
   11. Yingqiu Li

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     PKC-theta is known to regulate T cell activation, and this manuscript reveals a novel function of PKC-theta in the regulation of the nuclear pore complexes. The work by He and colleagues reveals that PKC-theta is recruited to the nuclear pore complex wherein it serves to regulate the assembly of key components of the RanBP2 subcomplex of the NPC, which in turn enables the translocation of AP1, NFkB and NFAT into the nucleus. However, these results need to be substantiated by additional experiments or by limiting the breath of the conclusions.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. Allosteric communication in class A β-lactamases occurs via cooperative coupling of loop dynamics

    This article has 13 authors:

    1. Ioannis Galdadas
    2. Shen Qu
    3. Ana Sofia F Oliveira
    4. Edgar Olehnovics
    5. Andrew R Mack
    6. Maria F Mojica
    7. Pratul K Agarwal
    8. Catherine L Tooke
    9. Francesco Luigi Gervasio
    10. James Spencer
    11. Robert A Bonomo
    12. Adrian J Mulholland
    13. Shozeb Haider

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This manuscript presents a computational study aiming to understand the allosteric signaling propagation pathway in two class-A beta-lactamases. The results of this study will be of interest to the readers in the fields of beta-lactamase, antibiotic resistance, and enzyme allostery.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewers #1, #2, and #3 agreed to share their names with the authors.)

    Reviewed by eLife