eLife

Latest preprint reviews

1. Asymptomatic Bordetella pertussis infections in a longitudinal cohort of young African infants and their mothers

   This article has 9 authors:

   1. Christopher J Gill
   2. Christian E Gunning
   3. William B MacLeod
   4. Lawrence Mwananyanda
   5. Donald M Thea
   6. Rachel C Pieciak
   7. Geoffrey Kwenda
   8. Zacharia Mupila
   9. Pejman Rohani

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Overall the reviewers were positive about this manuscript and the importance of this analysis being in identifying the role of asymptomatic transmitters in populations. There are some revisions that will be required and a number of areas for additional analyses and clarifications that would help the reader better put this manuscript in context.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

2. Enhanced functional detection of synaptic calcium-permeable AMPA receptors using intracellular NASPM

   This article has 6 authors:

   1. Ian Coombs
   2. Cécile Bats
   3. Craig A Sexton
   4. Dorota Studniarczyk
   5. Stuart G Cull-Candy
   6. Mark Farrant

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Here the authors identify that inclusion of intracellular NASPM can fully block Ca-permeable AMPA receptors regardless of association with auxiliary subunits. The distinction between Ca-permeable and Ca-impermeable AMPA receptors is critical to synaptic physiology, and thus these results will be of interest within the field of excitatory synaptic transmission. The data is of high quality and the experimental analysis is rigorous, but the key claim that the approach provides an unambiguous functional measure of CP-AMPAR prevalence has not been fully supported.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

3. Genetic integration of behavioural and endocrine components of the stress response

   This article has 9 authors:

   1. Thomas M Houslay
   2. Ryan L Earley
   3. Stephen J White
   4. Wiebke Lammers
   5. Andrew J Grimmer
   6. Laura M Travers
   7. Elizabeth L Johnson
   8. Andrew J Young
   9. Alastair Wilson

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is a timely paper on the genetic integration of behavioral and physiological components of the stress response in guppies. Using evolutionary quantitative genetic approaches, the authors show that genetic variation in the cortisol stress response is associated with genetic variation in stress-related behaviors. This result suggests that physiological and behavioral responses to stress should show correlated evolution in response to natural selection, which is of interest to evolutionary biologists and for animal welfare. The reviewers pointed out several conceptual and methodological issues with the definition of the phenotypes under study and and with the definition strong genetic integration.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

4. Whole-organism eQTL mapping at cellular resolution with single-cell sequencing

   This article has 6 authors:

   1. Eyal Ben-David
   2. James Boocock
   3. Longhua Guo
   4. Stefan Zdraljevic
   5. Joshua S. Bloom
   6. Leonid Kruglyak

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors use a pooled single cell sequencing approach to simultaneously genotype and phenotype C. elegans. This allows them to begin to query the genetic architecture of cell specific eQTLs in a multi-cellular organism.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. An agnostic study of associations between ABO and RhD blood group and phenome-wide disease risk

   This article has 5 authors:

   1. Torsten Dahlén
   2. Mark Clements
   3. Jingcheng Zhao
   4. Martin L Olsson
   5. Gustaf Edgren

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this manuscript, Dahlen et al. agnostically survey a large transfusion database in Sweden to investigate the association between ABO and RhD blood group and disease occurrence for a large number of clinical phenotypes. The data reported are purely epidemiological associations, with no direct insight into biological mechanism. Nonetheless, these data are a valuable resource for the research community, and offer the potential for a number of important biologic hypotheses and insights for investigation in the future.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

6. CEM500K, a large-scale heterogeneous unlabeled cellular electron microscopy image dataset for deep learning

   This article has 2 authors:

   1. Ryan Conrad
   2. Kedar Narayan

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes the curation of a training dataset that will be an important resource for developers of new segmentation and deep-learning algorithms for electron microscopy data. The small size of the dataset makes it easy to use, and its broad range of image modalities ensure that the model will be applicable in many situations, making it very useful for the community.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

7. Stress resets ancestral heritable small RNA responses

   This article has 4 authors:

   1. Leah Houri-Zeevi
   2. Guy Teichman
   3. Hila Gingold
   4. Oded Rechavi

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In humans, extreme stresses, such as famine, can trigger multi-generational physiological responses through altered metabolism. In C. elegans, environmental stresses, such as heat shock, can similarly promote changes in gene expression and physiology. Here, the authors convincingly show that environmental stress can alter small RNA populations in such a manner that can alter gene expression over multiple generations. The work is beginning to tease out some of the mechanisms by which non-genetic information can regulate descendants biology.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife, preLights

8. Impaired spatial learning and suppression of sharp wave ripples by cholinergic activation at the goal location

   This article has 4 authors:

   1. Przemyslaw Jarzebowski
   2. Clara S Tang
   3. Ole Paulsen
   4. Y Audrey Hay

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest for those interested in the roles of cholinergic projections from the medial septum and sharp wave-ripples on reward learning. The work provides compelling evidence showing that activation of septal cholinergic cells at reward zones suppresses sharp wave-ripples and impairs memory performance in freely behaving animals. The work extends our knowledge of the effect of medial septum cholinergic inputs on hippocampal dependent spatial memory formation.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

9. StormGraph: A graph-based algorithm for quantitative clustering analysis of diverse single-molecule localization microscopy data

   This article has 7 authors:

   1. Joshua M. Scurll
   2. Libin Abraham
   3. Da Wei Zheng
   4. Reza Tafteh
   5. Keng C. Chou
   6. Michael R. Gold
   7. Daniel Coombs

   Reviewed by eLife

10. Systemic inflammation recruits fast-acting anti-inflammatory innate myeloid progenitors from BM into lymphatics

    This article has 16 authors:

    1. Juana Serrano-Lopez
    2. Shailaja Hegde
    3. Sachin Kumar
    4. Josefina Serrano
    5. Jing Fang
    6. Ashley M. Wellendorf
    7. Paul A. Roche
    8. Yamileth Rangel
    9. Léolène J. Carrington
    10. Hartmut Geiger
    11. H. Leighton Grimes
    12. Sanjiv Luther
    13. Ivan Maillard
    14. Joaquin Sanchez-Garcia
    15. Daniel T. Starczynowski
    16. Jose A. Cancelas

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      The authors demonstrate that acute systemic inflammation induces a new system of rapid migration of granulocyte-macrophage progenitors and committed macrophage-dendritic progenitors but not other progenitors or stem cells from BM to lymphatic capillaries. The cells appear in the lymphatics earlier than in peripheral blood. This type of trafficking is triggered by LPS administration and is anti-inflammatory.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife