eLife

Latest preprint reviews

1. Activation of mTORC1 and c-Jun by Prohibitin1 loss in Schwann cells may link mitochondrial dysfunction to demyelination

   This article has 8 authors:

   1. Gustavo Della-Flora Nunes
   2. Emma R Wilson
   3. Edward Hurley
   4. Bin He
   5. Bert W O'Malley
   6. Yannick Poitelon
   7. Lawrence Wrabetz
   8. M Laura Feltri

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript builds upon the recent observation that Schwann cell (SC)-specific loss of the mitochondrial protein Prohibitin-1 results in a rapid, progressive demyelinating peripheral neuropathy in mice associated with mitochondrial dysfunction. They establish pathways as downstream effectors of mitochondrial dysfunction in Schwann cells. The authors provide a comprehensive evaluation of these pathways following the loss of Prophibitin-1 and identify JUN and mTORC1 as potential mediators of myelin disruption.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

2. Gjd2b-mediated gap junctions promote glutamatergic synapse formation and dendritic elaboration in Purkinje neurons

   This article has 7 authors:

   1. Sahana Sitaraman
   2. Gnaneshwar Yadav
   3. Vandana Agarwal
   4. Shaista Jabeen
   5. Shivangi Verma
   6. Meha Jadhav
   7. Vatsala Thirumalai

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Sitaraman and colleagues address the fundamental question of whether gap junctions facilitate the formation of chemical synapses. To do so they test the function of the gap junction protein, Gjd2b, in early stages of synaptogenesis in larval Zebrafish cerebellar Purkinje neurons. They provide convincing evidence that Gjd2b is necessary for the development of glutamatergic synapses and dendritic arbor growth Purkinje neurons in vivo and that CaMKII plays a role in regulating arbor development. This study will be an important contribution to our understanding of molecular and cellular mechanisms underlying brain development.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

3. Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner

   This article has 4 authors:

   1. Sun-Hee Hwang
   2. Bandarigoda N Somatilaka
   3. Kevin White
   4. Saikat Mukhopadhyay

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to scientists interested in the biology of the G-protein-coupled receptor Gpr161 and its ciliary regulation of Hedgehog (Hh) signaling. The phenotypes observed in a new GPR161 mutant mouse carrying a hypomorphic allele provide additional information showing which developing tissues are more sensitive to Gpr161 function. However, the data at this stage are insufficient to support the main novel conclusion: the ciliary function of Gpr161 is to regulate Gli3 repressor while the extra-ciliary function is to regulate Gli activator.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

4. Subcellular proteomics of dopamine neurons in the mouse brain

   This article has 6 authors:

   1. Benjamin D Hobson
   2. Se Joon Choi
   3. Eugene V Mosharov
   4. Rajesh K Soni
   5. David Sulzer
   6. Peter A Sims

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this work, the authors provide a useful compendium of proteins labeled within SNpc dopaminergic cells using a novel approach. AAV virus was used to conditional express the APEX2 enzyme in dopaminergic neurons (based on DAT-1 Cre genetic technology) to rapidly biotinylate nearby proteins in oriented sections of brain whereby the striatonigral circuit can be spatially parsed for proteomic dissection. In addition to providing a useful new database of proteins for investigators interested in this circuit, the results also provide a more general approach to examining a compartment proteome in neurons and what might be expected in that analysis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. Circadian regulation of vertebrate cone photoreceptor function

   This article has 6 authors:

   1. Jingjing Zang
   2. Matthias Gesemann
   3. Jennifer Keim
   4. Marijana Samardzija
   5. Christian Grimm
   6. Stephan CF Neuhauss

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Light and light perception are important factors that modulate several aspects of behavior and physiology in all animals, including humans. More specifically, the paper examines circadian cycling of phototransduction regulators in diurnal zebrafish and nocturnal mice, and links them to function at the level of ERGs. Interestingly, the transcriptional cycling is shifted between zebrafish and mice. This work is of relevance to vision researchers, but also of interest to a broader audience of behavioral (neuro)scientists and chronobiologists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

6. Loss of N1-methylation of G37 in tRNA induces ribosome stalling and reprograms gene expression

   This article has 8 authors:

   1. Isao Masuda
   2. Jae-Yeon Hwang
   3. Thomas Christian
   4. Sunita Maharjan
   5. Fuad Mohammad
   6. Howard Gamper
   7. Allen R Buskirk
   8. Ya-Ming Hou

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript provides evidence that loss of N1-methylation of G37 of tRNAs in bacteria on depletion of TrmD results in defects in aminoacylation and peptidyl-transfer, leading to ribosome stalling and phenotypes indicating activation of the stringent response.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Aorta smooth muscle-on-a-chip reveals impaired mitochondrial dynamics as a therapeutic target for aortic aneurysm in bicuspid aortic valve disease

   This article has 18 authors:

   1. Mieradilijiang Abudupataer
   2. Shichao Zhu
   3. Shiqiang Yan
   4. Kehua Xu
   5. Jingjing Zhang
   6. Shaman Luo
   7. Wenrui Ma
   8. Md Fazle Alam
   9. Yuyi Tang
   10. Hui Huang
   11. Nan Chen
   12. Li Wang
   13. Guoquan Yan
   14. Jun Li
   15. Hao Lai
   16. Chunsheng Wang
   17. Kai Zhu
   18. Weijia Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors developed an aorta-on-a-chip system to investigate the potential mechanism of Bicuspid Aortic Valve-Thoracic Aortic Aneurysm, BAV-TAA, and to test candidate pharmacologic agents. This study is of broad interest to readers in the field of lab-on-a-chip, biomedicine, biomaterials, etc. However, some important details regarding the development of the model are missing, as are some control data. The authors need to discuss the limitations of this model, such as the inability of their on-a-chip model to recapitulate aortic changes associated with complex pathologic processes such as ECM degradation, inflammation, etc, and discuss the importance of follow-up studies in in vivo models.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

8. Fibrinogen αC-subregions critically contribute blood clot fibre growth, mechanical stability, and resistance to fibrinolysis

   This article has 13 authors:

   1. Helen R McPherson
   2. Cedric Duval
   3. Stephen R Baker
   4. Matthew S Hindle
   5. Lih T Cheah
   6. Nathan L Asquith
   7. Marco M Domingues
   8. Victoria C Ridger
   9. Simon DA Connell
   10. Khalid M Naseem
   11. Helen Philippou
   12. Ramzi A Ajjan
   13. Robert AS Ariëns

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to a broad audience in the blood coagulation and fibrinolysis field. Previously undescribed roles in a range of blood clot properties are attributed to a region of the clotting protein fibrinogen, using state-of-the-art methodology. The data support the main conclusions of the paper, open new avenues of investigation for understanding clot properties, and have clinical implications.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. Functional independence of endogenous μ- and δ-opioid receptors co-expressed in cholinergic interneurons

   This article has 4 authors:

   1. Seksiri Arttamangkul
   2. Emily J Platt
   3. James Carroll
   4. David Farrens

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study is significant because it addresses a debated question in the field about whether different opioid receptor subtypes expressed in the same cells must function as a unit or function independently. Here the authors show that while mu and delta opioid receptors each signals in a similar manner in response to specific treatments, their interactions are largely independent of one another in modulating the firing and regulation by desensitization and internalization mechanisms in striatal cholinergic interneurons.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

10. Principles of RNA recruitment to viral ribonucleoprotein condensates in a segmented dsRNA virus

    This article has 7 authors:

    1. Sebastian Strauss
    2. Julia Acker
    3. Guido Papa
    4. Daniel Desirò
    5. Florian Schueder
    6. Alexander Borodavka
    7. Ralf Jungmann

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This study will be of interest to virologists and those working on RNA-protein particles. Strauss and colleagues studied the mechanism of RNA recruitment to ribonucleoprotein condensates using rotavirus. Using multiplexed DNA-barcorded smFISH and DNA-PAINT for direct visualization of the RNP condensates in cells, they observe the early onset of viral transcript oligomerization before the formation of viroplasms and the process of enrichment in RNP condensates. They were able to image all eleven transcripts in an RNP condensate and to quantify the amounts of these transcripts. Based on these findings, the authors suggest a selective RNA enrichment mechanism of rotavirus. The experiments are nicely executed, with good controls.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife