eLife

Latest preprint reviews

1. GFPT2/GFAT2 and AMDHD2 act in tandem to control the hexosamine pathway

   This article has 11 authors:

   1. Virginia Kroef
   2. Sabine Ruegenberg
   3. Moritz Horn
   4. Kira Allmeroth
   5. Lena Ebert
   6. Seyma Bozkus
   7. Stephan Miethe
   8. Ulrich Elling
   9. Bernhard Schermer
   10. Ulrich Baumann
   11. Martin Sebastian Denzel

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes an interesting regulation of the hexosamine biosynthetic pathway (HBP) that is relative specific to the mouse embryonic stem cells (mESC). HBP produces UDP-N-acetylglucosamine, which is used in various protein glycosylation events, thus regulating many biological pathways. Understanding this pathway and its regulation is thus of fundamental significance.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

2. Neural effects of continuous theta-burst stimulation in macaque parietal neurons

   This article has 4 authors:

   1. Maria C Romero
   2. Lara Merken
   3. Peter Janssen
   4. Marco Davare

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The experiments reported here directly assess the impact of theta burst transcranial magnetic stimulation on neuronal activity. It will be of high potential interest to researchers using non-invasive brain stimulation. Although the results are highly intriguing, several methodological issues limit the inferences that can be drawn from the data as currently presented.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

3. An evidence-based 3D reconstruction of Asteroxylon mackiei, the most complex plant preserved from the Rhynie chert

   This article has 6 authors:

   1. Alexander J Hetherington
   2. Siobhán L Bridson
   3. Anna Lee Jones
   4. Hagen Hass
   5. Hans Kerp
   6. Liam Dolan

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to readers in the field of plant evolutionary biology and paleontology, paleobotany in particular. Using novel 3D reconstruction techniques, the authors provide the first evidence-based reconstruction of Asteroxylon mackiei, the Early Devonian Rhynie chert plant known for a century, and demonstrate that it possessed an extinct pattern of root development transitional to the evolution of true roots in modern club-mosses. The use of multiple lines of evidence and 3D reconstructions based on serial sections of petrified materials provides compelling support for the key conclusions of the paper.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewers #1, #2, and #3 agreed to share their names with the authors.)

   Reviewed by eLife

4. The mechanism of MICU-dependent gating of the mitochondrial Ca2+uniporter

   This article has 8 authors:

   1. Vivek Garg
   2. Junji Suzuki
   3. Ishan Paranjpe
   4. Tiffany Unsulangi
   5. Liron Boyman
   6. Lorin S Milescu
   7. W Jonathan Lederer
   8. Yuriy Kirichok

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper examines the roles and mechanisms of how subunits of the mitochondrial calcium uniporter complex (MCUcx) regulate calcium uptake by mitochondria, a process that serves to match the rate of ATP generation to cellular metabolic needs. Based on direct electrophysiological recordings of MCUcx, the authors find that the MICU1 subunit potentiates channel activity in a calcium-dependent manner but does not block the channel at low calcium levels, challenging current models of MCU regulation. This work will be of significant interest to biophysicists and cell biologists interested in mitochondrial biology, bioenergetics, and ion channel and calcium signaling mechanisms.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. Patterns of within-host genetic diversity in SARS-CoV-2

   This article has 41 authors:

   1. Gerry Tonkin-Hill
   2. Inigo Martincorena
   3. Roberto Amato
   4. Andrew RJ Lawson
   5. Moritz Gerstung
   6. Ian Johnston
   7. David K Jackson
   8. Naomi Park
   9. Stefanie V Lensing
   10. Michael A Quail
   11. Sónia Gonçalves
   12. Cristina Ariani
   13. Michael Spencer Chapman
   14. William L Hamilton
   15. Luke W Meredith
   16. Grant Hall
   17. Aminu S Jahun
   18. Yasmin Chaudhry
   19. Myra Hosmillo
   20. Malte L Pinckert
   21. Iliana Georgana
   22. Anna Yakovleva
   23. Laura G Caller
   24. Sarah L Caddy
   25. Theresa Feltwell
   26. Fahad A Khokhar
   27. Charlotte J Houldcroft
   28. Martin D Curran
   29. Surendra Parmar
   30. The COVID-19 Genomics UK (COG-UK) Consortium
   31. Alex Alderton
   32. Rachel Nelson
   33. Ewan M Harrison
   34. John Sillitoe
   35. Stephen D Bentley
   36. Jeffrey C Barrett
   37. M Estee Torok
   38. Ian G Goodfellow
   39. Cordelia Langford
   40. Dominic Kwiatkowski
   41. Wellcome Sanger Institute COVID-19 Surveillance Team

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Tonkin-Hill and colleagues present a large set of deep sequencing data from acute SARS-CoV-2 infections with each sample sequenced in duplicate. They use these data to characterize the within-host mutational patterns and diversity and relate them to SARS-CoV-2 diversity in consensus sequences sampled around the globe. It further allows understanding how this variation can or cannot be used to understand transmission dynamics and other applications in genomic epidemiology.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife, ScreenIT

6. The role of ventromedial prefrontal cortex in reward valuation and future thinking during intertemporal choice

   This article has 10 authors:

   1. Elisa Ciaramelli
   2. Flavia De Luca
   3. Donna Kwan
   4. Jenkin Mok
   5. Francesca Bianconi
   6. Violetta Knyagnytska
   7. Carl Craver
   8. Leonard Green
   9. Joel Myerson
   10. R Shayna Rosenbaum

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper provides important data on the contribution of the vmPFC to temporal discounting, by showing differential modulation of two well-known effects in inter-temporal choice (magnitude effect, episodic future thinking). VMPFC damage abolished the magnitude effect, but not a modulation of discounting via future thinking, a finding of interest to cognitive neuroscientists working on prospection, decision-making and executive control.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

7. Pathogen invasion-dependent tissue reservoirs and plasmid-encoded antibiotic degradation boost plasmid spread in the gut

   This article has 12 authors:

   1. Erik Bakkeren
   2. Joana Anuschka Herter
   3. Jana Sanne Huisman
   4. Yves Steiger
   5. Ersin Gül
   6. Joshua Patrick Mark Newson
   7. Alexander Oliver Brachmann
   8. Jörn Piel
   9. Roland Regoes
   10. Sebastian Bonhoeffer
   11. Médéric Diard
   12. Wolf-Dietrich Hardt

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work describes an important feature of within-host acquisition of antibiotic resistance. This is a follow-up to their recent publication (Bakkeren et al. 2019), and complements their finding of persister cells in the tissues, to show that also chronic, tissue residing bacteria can provide plasmid tissue reservoirs. The experiments the authors performed are elegant and timely. This manuscript will be of interest to readers in the fields of infection biology, plasmid ecology, gut microbiomes, and antimicrobial resistance.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

8. A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite

   This article has 17 authors:

   1. Meret Huber
   2. Thomas Roder
   3. Sandra Irmisch
   4. Alexander Riedel
   5. Saskia Gablenz
   6. Julia Fricke
   7. Peter Rahfeld
   8. Michael Reichelt
   9. Christian Paetz
   10. Nicole Liechti
   11. Lingfei Hu
   12. Zoe Bont
   13. Ye Meng
   14. Wei Huang
   15. Christelle AM Robert
   16. Jonathan Gershenzon
   17. Matthias Erb

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to a broad audience interested in the coevolutionary arms race between plants and their herbivores. In a thoroughly investigated case study, the detoxification strategy of cockchafer larvae towards the major defensive compound of one of their preferred host plants, dandelion, is revealed and effects on the behavior of the larvae are described.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

9. Cell-to-cell heterogeneity in Sox2 and Bra expression guides progenitor motility and destiny

   This article has 10 authors:

   1. Michèle Romanos
   2. Guillaume Allio
   3. Myriam Roussigné
   4. Léa Combres
   5. Nathalie Escalas
   6. Cathy Soula
   7. François Médevielle
   8. Benjamin Steventon
   9. Ariane Trescases
   10. Bertrand Bénazéraf

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study is of potential interest to biologists interested in developmental patterning and the link between cellular identity and behavior. The authors perform experiments in the progenitor zone of avian embryos to propose that heterogeneity of cellular behaviors may drive morphogenesis and underlie cell fate choices. The work is nicely done but might need some additional experimental validation of the proposed hypothesis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

10. A proteome-wide genetic investigation identifies several SARS-CoV-2-exploited host targets of clinical relevance

    This article has 18 authors:

    1. Mohd Anisul
    2. Jarrod Shilts
    3. Jeremy Schwartzentruber
    4. James Hayhurst
    5. Annalisa Buniello
    6. Elmutaz Shaikho Elhaj Mohammed
    7. Jie Zheng
    8. Michael Holmes
    9. David Ochoa
    10. Miguel Carmona
    11. Joseph Maranville
    12. Tom R Gaunt
    13. Valur Emilsson
    14. Vilmundur Gudnason
    15. Ellen M McDonagh
    16. Gavin J Wright
    17. Maya Ghoussaini
    18. Ian Dunham

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This study analyzes publicly available genomic and proteomic data to identify host proteins that may be involved in regulation of COVID-19 susceptibility and severity. Several known and new targets are identified, further revealing the complexity of host genetic variation in COVID-19 disease.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife, ScreenIT