eLife

Latest preprint reviews

1. SoxB1 family members inhibit Wnt signaling to promote maturation and deposition of stable neuromasts by the zebrafish Posterior Lateral Line primordium

   This article has 7 authors:

   1. Greg Palardy
   2. Kyeong-won Yoo
   3. Sana Fatma
   4. Abhishek Mukherjee
   5. Chongmin Wang
   6. Priyanka Ravi
   7. Ajay B Chitnis

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study explores the regulation of collective cell migration and tissue patterning in the zebrafish posterior lateral line primordium by SoxB1 transcription factors. The authors provide evidence that SoxB1 genes interact with Wnt and Fgf signaling pathways to control neuromast deposition and spacing, a process central to sensory organ development. The work offers mechanistic insight into the self-organization of migrating tissues and adds to the understanding of how transcriptional networks integrate with signaling pathways during morphogenesis. However, the strength of the evidence supporting several key conclusions is incomplete due to insufficient validation of mutant and knockdown tools, lack of quantitative analysis, and unclear experimental design details; additional quantification and more rigorous verification of gene knockdown or loss-of-function tools are needed to support the proposed model.

   Reviewed by eLife

2. Correlates of protection against African swine fever virus identified by a systems immunology approach

   This article has 12 authors:

   1. Kirill Lotonin
   2. Francisco Brito
   3. Kemal Mehinagic
   4. Obdulio García-Nicolás
   5. Matthias Liniger
   6. Noelle Donzé
   7. Sylvie Python
   8. Stephanie Talker
   9. Tosca Ploegaert
   10. Nicolas Ruggli
   11. Charaf Benarafa
   12. Artur Summerfield

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable findings regarding potential correlates of protection against the African swine fever virus. The evidence supporting the claims is solid, and the results are highly relevant to the field. Further analysis using larger number of animals and other virus strains will help validate the importance of these findings and assess the relevance of the immune parameters associated with protection. The work will be of broad interest to veterinary immunologists, and particularly those working on African swine fever.

   Reviewed by eLife

3. Probing relaxed myosin states in hypertrophic cardiomyopathy by second harmonic-generation microscopy

   This article has 17 authors:

   1. Giulia Arecchi
   2. Marica Dente
   3. Weikang Ma
   4. Beatrice Scellini
   5. Nicoletta Piroddi
   6. Marina Scardigli
   7. Jingyuan Yu
   8. Jing Zhao
   9. Riccardo Cicchi
   10. Ryo Kinegawa
   11. Caroline Muellenbroich
   12. Corrado Poggesi
   13. Cecilia Ferrantini
   14. Thomas C Irving
   15. Michael Regnier
   16. Leonardo Sacconi
   17. Chiara Tesi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Arecchi et al. demonstrate that polarized second-harmonic generation microscopy can be used to probe the ON/OFF states of myosin in both permeabilized and intact muscle, making this key measurement accessible to a greater number of labs. This has the potential to help with the study of disease-causing mutations and our understanding of drug function. The methodology is well defined, and the results are important; however, whilst this is overall a convincing study, there are some limitations to the interpretation of the data.

   Reviewed by eLife

4. Phenylhydrazone-based endoplasmic reticulum proteostasis regulator compounds with enhanced biological activity

   This article has 11 authors:

   1. Gabriel M Kline
   2. Lisa Boinon
   3. Adrian Guerrero
   4. Sergei Kutseikin
   5. Gabrielle Cruz
   6. Marnie P Williams
   7. Ryan J Paxman
   8. William E Balch
   9. Jeffery W Kelly
   10. Tingwei Mu
   11. R Luke Wiseman

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study reports the important development and characterization of next-generation analogs of the molecule AA263, which was previously identified for its ability to promote adaptive ER proteostasis remodeling. The evidence supporting the conclusions is convincing, with rigorous assays used to benchmark the changes in potency and efficacy of the AA263 analogs as well as AA263 targets. The ability of AA263 analogs to restore the loss of function associated with disease-associated proteins prone to misfolding will be of interest to pharmacologists, chemical biologists, and cell biologists, as well as those working on protein misfolding disorders.

   Reviewed by eLife

5. Convergent Cellular Adaptation to Freeze-Thaw Stress via a Quiescence-like State in Yeast

   This article has 9 authors:

   1. Charuhansini Tvishamayi
   2. Farhan Ali
   3. Nandita Chaturvedi
   4. Nithila Madhu-Kumar
   5. Zeenat Rashida
   6. Chandan Muni Reddy
   7. Ankita Ray
   8. Stephan Herminghaus
   9. Shashi Thutupalli

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insight into stress biology by showing that yeast populations can rapidly evolve a trehalose producing resting state that substantially improves survival and rapid regrowth after freeze-thaw. This finding is consistent with the role of trehalose metabolism as a biophysical adaptation that is broadly relevant to the community working on environmental resilience and dormancy. The evidence is convincing: the authors integrate experimental evolution, cell-level biophysical measurements, and modelling in a mutually reinforcing manner.

   Reviewed by eLife

6. SMC complex unidirectionally translocates DNA by coupling segment capture with an asymmetric kleisin path

   This article has 4 authors:

   1. Masataka Yamauchi
   2. Giovanni Bruno Brandani
   3. Tsuyoshi Terakawa
   4. Shoji Takada

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study presents a well-constructed multiscale simulation framework to investigate ATP-driven DNA translocation by prokaryotic SMC complexes, supporting a segment-capture mechanism. The strength of evidence is convincing, highlighting the necessity of a precise balance between electrostatic interactions and hydrogen bonding, as well as the critical role of kleisin asymmetry in ensuring unidirectional movement.

   Reviewed by eLife

7. Striatal Crosstalk Between Dopamine and Serotonin Systems

   This article has 3 authors:

   1. Yu Liu
   2. Juan Enriquez Traba
   3. Christian Lüscher

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful study employs optogenetics, genetically-encoded dopamine and serotonin sensors, and patch-clamp electrophysiology to investigate modulations of neurotransmitter release between striatal dopamine and serotonin neurons - a topic of interest to neuroscientists studying the basal ganglia. The results suggest that the dopamine and serotonin systems operate largely in parallel, with the activation of serotonin neurons resulting in a small, transient dopamine release. The authors suggest that this interaction occurs via glutamate release in the ventral tegmental area, findings that are closely related to previous work. Some conclusions are incomplete requiring larger samples-sizes and controls.

   Reviewed by eLife

8. Profiling presynaptic scaffolds using split-GFP reconstitution reveals cell-type-specific spatial configurations in the fly brain

   This article has 6 authors:

   1. Hongyang Wu
   2. Yoh Maekawa
   3. Sayaka Eno
   4. Shu Kondo
   5. Nobuhiro Yamagata
   6. Hiromu Tanimoto

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important work introduces a splitGFP-based labeling tool with an analysis pipeline for the synaptic scaffold protein bruchpilot, with tests in the adult Drosophila mushroom bodies, a learning center in the Drosophila brain. The evidence supporting the conclusions is convincing.

   Reviewed by eLife

9. Developmental constraints mediate the reversal of temperature effects on the autumn phenology of European beech after the summer solstice

   This article has 9 authors:

   1. Dominic Rebindaine
   2. Thomas W Crowther
   3. Susanne S Renner
   4. Zhaofei Wu
   5. Yibiao Zou
   6. Lidong Mo
   7. Haozhi Ma
   8. Raymo Bucher
   9. Constantin M Zohner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This article presents valuable findings on how the timing of cooling affects autumn bud set in European beech saplings. The study leverages extensive experimental data and provides an interesting conceptual framework for the various ways in which warming can affect bud set timing. The statistical analysis is very well considered, while indicating some factors that may temper the authors' claims. The factorial experiments offer solid support.

   Reviewed by eLife

10. Astrocytic modulation of population encoding in mouse visual cortex via GABA transporter 3 revealed by multiplexed CRISPR/Cas9 gene editing

    This article has 12 authors:

    1. Jiho Park
    2. Grayson O Sipe
    3. Xin Tang
    4. Prachi Ojha
    5. Giselle Fernandes
    6. Yi Ning Leow
    7. Caroline Zhang
    8. Yuma Osako
    9. Arundhati Natesan
    10. Gabrielle T Drummond
    11. Rudolf Jaenisch
    12. Mriganka Sur

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      In this manuscript, Park et al. developed a multiplexed CRISPR construct to genetically ablate the GABA transporter GAT3 in the mouse visual cortex, with effects on population-level neuronal activity. This work is important, as it sheds light on how GAT3 controls the processing of visual information. The findings are compelling, leveraging state-of-the-art gene CRISPR/Cas9, in vivo two-photon laser scanning microscopy, and advanced statistical modeling.

    Reviewed by eLife