eLife

Latest preprint reviews

1. Low-affinity integrin states have faster ligand-binding kinetics than the high-affinity state

   This article has 3 authors:

   1. Jing Li
   2. Jiabin Yan
   3. Timothy A Springer

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Li, Yan and Springer report ligand binding on- and off-rates for three different conformations of α4β1 as well as α5β1 integrin. This is the first report that provides these numbers, which are important to understand the 'mode of integrin activation'. The study is - from a technical stand point - flawlessly performed and the calculated data is in perfect agreement with the previously published data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

2. A test of the pioneer factor hypothesis using ectopic liver gene activation

   This article has 3 authors:

   1. Jeffrey L Hansen
   2. Kaiser J Loell
   3. Barak A Cohen

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     How transcription factors access their DNA binding motifs in chromatin and cooperate with other transcription factors in DNA binding remains a contentious question. It is clear that some transcription factors ("pioneer transcription factors") play a dominant role in opening chromatin during development and reprogramming, but it has also been clear that the ability of transcription factors to do so lies on a spectrum, that pioneer transcription factors may mutually interact with other transcription factors in their pioneering activity and that their mode of binding is still poorly understood. This manuscript's presentation attempts to refute an overly simplified pioneer factor hypothesis. Overall, this is an important topic and the authors use a good experimental approach, but the analyses are limited and the interpretation too simplified.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

3. Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans

   This article has 5 authors:

   1. Ian F Price
   2. Hannah L Hertz
   3. Benjamin Pastore
   4. Jillian Wagner
   5. Wen Tang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors use proximity labeling and genetic experiments to identify and functionally characterize new components of C. elegans P granules. The conclusions of the paper are well-supported by the data. This work will be of broad interest to developmental biologists, particularly those interested in the formation and function of germ cells.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

4. Effects of arousal and movement on secondary somatosensory and visual thalamus

   This article has 4 authors:

   1. Gordon H Petty
   2. Amanda K Kinnischtzke
   3. Y Kate Hong
   4. Randy M Bruno

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors show that secondary thalamic region POm provides information on slow as opposed to rapid changes in whisker angular position; these appear to be secondary to changes in the animal's behavioral state. The authors find similar state dependent activity in LP, a higher level visual thalamic nucleus. This is a timely study in that many labs have observed state-dependent activity throughout the cortex and thalamus, but the mechanisms of this activity are incompletely understood. This study brings us closer to revealing the source of this signal by ruling out some of the likely candidates, such as reafferent signals and cortical feedback.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. Specialized neurons in the right habenula mediate response to aversive olfactory cues

   This article has 5 authors:

   1. Jung-Hwa Choi
   2. Erik R Duboue
   3. Michelle Macurak
   4. Jean-Michel Chanchu
   5. Marnie E Halpern

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The three reviewers have appreciated the novelty and originality of the study, but note that improved visualization, quantifications and statistical analyses will be necessary to fully support the conclusions of the manuscript. Without performing these quantifications and statistical tests for all figures as detailed below, the magnitude and significance of reported effects are not clear, nor do they take into account the variability of the measures and the dependence of some of the measures.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

6. We couldn't get details of this article at this time:
   10.1101/2021.06.28.450168

7. Metabolic requirement for GOT2 in pancreatic cancer depends on environmental context

   This article has 31 authors:

   1. Samuel A Kerk
   2. Lin Lin
   3. Amy L Myers
   4. Damien J Sutton
   5. Anthony Andren
   6. Peter Sajjakulnukit
   7. Li Zhang
   8. Yaqing Zhang
   9. Jennifer A Jiménez
   10. Barbara S Nelson
   11. Brandon Chen
   12. Anthony Robinson
   13. Galloway Thurston
   14. Samantha B Kemp
   15. Nina G Steele
   16. Megan T Hoffman
   17. Hui-Ju Wen
   18. Daniel Long
   19. Sarah E Ackenhusen
   20. Johanna Ramos
   21. Xiaohua Gao
   22. Zeribe C Nwosu
   23. Stefanie Galban
   24. Christopher J Halbrook
   25. David B Lombard
   26. David R Piwnica-Worms
   27. Haoqiang Ying
   28. Marina Pasca di Magliano
   29. Howard C Crawford
   30. Yatrik M Shah
   31. Costas A Lyssiotis

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper investigating specific metabolic dependencies of pancreatic cancer cells growing in vitro and in vivo will be of interest to scientists in the field of cancer metabolism. The data reveal that cancer-associated stromal cells can play an important role supporting the redox state of cancer cells cultured in vitro, but at present the data do not support the conclusion that this mechanism controls the metabolic resilience of cancer cells growing in vivo and alternate hypotheses remain to be addressed.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

8. An Algorithmic Barrier to Neural Circuit Understanding

   This article has 1 author:

   1. Venkatakrishnan Ramaswamy

   Reviewed by eLife

9. A mechano-osmotic feedback couples cell volume to the rate of cell deformation

   This article has 16 authors:

   1. Larisa Venkova
   2. Amit Singh Vishen
   3. Sergio Lembo
   4. Nishit Srivastava
   5. Baptiste Duchamp
   6. Artur Ruppel
   7. Alice Williart
   8. Stéphane Vassilopoulos
   9. Alexandre Deslys
   10. Juan Manuel Garcia Arcos
   11. Alba Diz-Muñoz
   12. Martial Balland
   13. Jean-François Joanny
   14. Damien Cuvelier
   15. Pierre Sens
   16. Matthieu Piel

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The paper by Venkova et al. is a comprehensive study of mammalian cell volume dynamics during the common cellular process of adhesion and spreading on a flat substrate, osmotic changes, and mechanical confinement. The paper reveals a complex interplay between cell water/ion regulation, cytoskeletal processes, and mechanical deformation of the cell. The topic is important in cell physiology and should be of considerable interest to cell biologists, mechanobiologists and biophysicists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. Adverse childhood experiences and resilience among adult women: A population-based study

    This article has 16 authors:

    1. Hilda Björk Daníelsdóttir
    2. Thor Aspelund
    3. Edda Bjork Thordardottir
    4. Katja Fall
    5. Fang Fang
    6. Gunnar Tómasson
    7. Harpa Rúnarsdóttir
    8. Qian Yang
    9. Karmel W Choi
    10. Beatrice Kennedy
    11. Thorhildur Halldorsdottir
    12. Donghao Lu
    13. Huan Song
    14. Jóhanna Jakobsdóttir
    15. Arna Hauksdóttir
    16. Unnur Anna Valdimarsdóttir

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This is one of the larger studies investigating the impact of adverse experiences during childhood on adult psychological and psychiatric resilience. Using data from an ongoing cohort study on Icelandic women, Daníelsdóttir and colleagues reported that in the face of accumulated adverse childhood events the prevalence of resilience declines, which supports earlier studies suggesting that resilience is not invincibility. Although the data are limited to women within gender binarism and the operationalization of resilience concept could be improved, the quality of the data, (e.g., sample size), justifies the authors' conclusion that the one way to improve resilience in adulthood lies in facilitating the quality of life in childhood. This paper has the potential to make an important contribution to raising awareness of the adverse childhood experiences and their impact on resiliency, which is of interest to those working on childhood adversity and resilience.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife