eLife

Latest preprint reviews

1. Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives

   This article has 3 authors:

   1. Ezra Lencer
   2. Rytis Prekeris
   3. Kristin Bruk Artinger

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript is of interest to researchers interested in specification and differentiation of the neural crest. This work presents a small single-cell RNAseq dataset from zebrafish trunk neural crest cells during the early stages of migration that identifies the subpopulations of trunk neural crest cells, new genetic markers and a subset of Rohon-Beard neurons. The paper confirms and extends previous work and reports expression of differentiated pigment cell types in the pre-migratory neural crest populations.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

2. The use of non-functional clonotypes as a natural calibrator for quantitative bias correction in adaptive immune receptor repertoire profiling

   This article has 8 authors:

   1. Anastasia O Smirnova
   2. Anna M Miroshnichenkova
   3. Yulia V Olshanskaya
   4. Michael A Maschan
   5. Yuri B Lebedev
   6. Dmitriy M Chudakov
   7. Ilgar Z Mamedov
   8. Alexander Komkov

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper describes a newly developed, publicly available algorithm (iROAR) that was tested on pre-exisiting datasets and is of interest to T and B cell immunologists who perform repertoire analysis via multiplex PCR based techniques. iROAR utilises naturally occurring non-functional sequences to improve and partially correct the amplification bias inherent in multiplex PCR based sequencing technologies.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

3. Tracking the relation between gist and item memory over the course of long-term memory consolidation

   This article has 4 authors:

   1. Tima Zeng
   2. Alexa Tompary
   3. Anna C Schapiro
   4. Sharon L Thompson-Schill

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to psychologists and neuroscientist investigating systems memory consolidation. It describes an experimental protocol that allows precise, quantitative behavioural measurements to assess the development and interactions of item and gist memory traces over extended time periods. The study design and hypotheses are elegant and bring together ideas from several other fields of cognitive psychology (working memory, category learning). However, additional analyses, and in particular, comparison of some simple computational models, are needed before the conclusions are justified.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

4. Natural variation in the consequences of gene overexpression and its implications for evolutionary trajectories

   This article has 5 authors:

   1. DeElegant Robinson
   2. Michael Place
   3. James Hose
   4. Adam Jochem
   5. Audrey P Gasch

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study investigates the effect of copy number variants across all genes in Saccharomyces cerevisiae, and the variation across different genetic backgrounds. Interestingly, apart from universal effects common to most of the genetic backgrounds, the authors also report strain-specific effects related to gene copy number variants.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. Decoding locomotion from population neural activity in moving C. elegans

   This article has 9 authors:

   1. Kelsey M Hallinen
   2. Ross Dempsey
   3. Monika Scholz
   4. Xinwei Yu
   5. Ashley Linder
   6. Francesco Randi
   7. Anuj K Sharma
   8. Joshua W Shaevitz
   9. Andrew M Leifer

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to a wide range of systems neuroscientists seeking to understanding the relationship between neuronal activity and behavior. Building on previous technical advances in brain-wide imaging of neuronal activity (Ca signals) in freely moving animals (Caenorhabditis elegans), it demonstrates that a linear regression model is sufficient reconstruct key parameters of locomotion - velocity and body curvature - from the imaging data and documents differences in activity between freely moving and immobilized worms.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. Cortical magnification in human visual cortex parallels task performance around the visual field

   This article has 5 authors:

   1. Noah C Benson
   2. Eline R Kupers
   3. Antoine Barbot
   4. Marisa Carrasco
   5. Jonathan Winawer

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript is of broad interest to readers in the field of human vision and its cortical topography as well as behavioral and genetic links. The investigation of the neurobiological basis of visual task performance asymmetries represents an important contribution to our understanding of how visual system architecture shapes perception. The key claims of the manuscript are well supported by the data, and the approaches used are thoughtful and rigorous.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewers #1, #2, and #3 agreed to share their names with the authors.)

   Reviewed by eLife

7. GFPT2/GFAT2 and AMDHD2 act in tandem to control the hexosamine pathway

   This article has 11 authors:

   1. Virginia Kroef
   2. Sabine Ruegenberg
   3. Moritz Horn
   4. Kira Allmeroth
   5. Lena Ebert
   6. Seyma Bozkus
   7. Stephan Miethe
   8. Ulrich Elling
   9. Bernhard Schermer
   10. Ulrich Baumann
   11. Martin Sebastian Denzel

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes an interesting regulation of the hexosamine biosynthetic pathway (HBP) that is relative specific to the mouse embryonic stem cells (mESC). HBP produces UDP-N-acetylglucosamine, which is used in various protein glycosylation events, thus regulating many biological pathways. Understanding this pathway and its regulation is thus of fundamental significance.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Neural effects of continuous theta-burst stimulation in macaque parietal neurons

   This article has 4 authors:

   1. Maria C Romero
   2. Lara Merken
   3. Peter Janssen
   4. Marco Davare

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The experiments reported here directly assess the impact of theta burst transcranial magnetic stimulation on neuronal activity. It will be of high potential interest to researchers using non-invasive brain stimulation. Although the results are highly intriguing, several methodological issues limit the inferences that can be drawn from the data as currently presented.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. An evidence-based 3D reconstruction of Asteroxylon mackiei, the most complex plant preserved from the Rhynie chert

   This article has 6 authors:

   1. Alexander J Hetherington
   2. Siobhán L Bridson
   3. Anna Lee Jones
   4. Hagen Hass
   5. Hans Kerp
   6. Liam Dolan

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to readers in the field of plant evolutionary biology and paleontology, paleobotany in particular. Using novel 3D reconstruction techniques, the authors provide the first evidence-based reconstruction of Asteroxylon mackiei, the Early Devonian Rhynie chert plant known for a century, and demonstrate that it possessed an extinct pattern of root development transitional to the evolution of true roots in modern club-mosses. The use of multiple lines of evidence and 3D reconstructions based on serial sections of petrified materials provides compelling support for the key conclusions of the paper.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewers #1, #2, and #3 agreed to share their names with the authors.)

   Reviewed by eLife

10. The mechanism of MICU-dependent gating of the mitochondrial Ca2+uniporter

    This article has 8 authors:

    1. Vivek Garg
    2. Junji Suzuki
    3. Ishan Paranjpe
    4. Tiffany Unsulangi
    5. Liron Boyman
    6. Lorin S Milescu
    7. W Jonathan Lederer
    8. Yuriy Kirichok

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This paper examines the roles and mechanisms of how subunits of the mitochondrial calcium uniporter complex (MCUcx) regulate calcium uptake by mitochondria, a process that serves to match the rate of ATP generation to cellular metabolic needs. Based on direct electrophysiological recordings of MCUcx, the authors find that the MICU1 subunit potentiates channel activity in a calcium-dependent manner but does not block the channel at low calcium levels, challenging current models of MCU regulation. This work will be of significant interest to biophysicists and cell biologists interested in mitochondrial biology, bioenergetics, and ion channel and calcium signaling mechanisms.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

    Reviewed by eLife