eLife

Latest preprint reviews

1. Patterns of within-host genetic diversity in SARS-CoV-2

   This article has 41 authors:

   1. Gerry Tonkin-Hill
   2. Inigo Martincorena
   3. Roberto Amato
   4. Andrew RJ Lawson
   5. Moritz Gerstung
   6. Ian Johnston
   7. David K Jackson
   8. Naomi Park
   9. Stefanie V Lensing
   10. Michael A Quail
   11. Sónia Gonçalves
   12. Cristina Ariani
   13. Michael Spencer Chapman
   14. William L Hamilton
   15. Luke W Meredith
   16. Grant Hall
   17. Aminu S Jahun
   18. Yasmin Chaudhry
   19. Myra Hosmillo
   20. Malte L Pinckert
   21. Iliana Georgana
   22. Anna Yakovleva
   23. Laura G Caller
   24. Sarah L Caddy
   25. Theresa Feltwell
   26. Fahad A Khokhar
   27. Charlotte J Houldcroft
   28. Martin D Curran
   29. Surendra Parmar
   30. The COVID-19 Genomics UK (COG-UK) Consortium
   31. Alex Alderton
   32. Rachel Nelson
   33. Ewan M Harrison
   34. John Sillitoe
   35. Stephen D Bentley
   36. Jeffrey C Barrett
   37. M Estee Torok
   38. Ian G Goodfellow
   39. Cordelia Langford
   40. Dominic Kwiatkowski
   41. Wellcome Sanger Institute COVID-19 Surveillance Team

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Tonkin-Hill and colleagues present a large set of deep sequencing data from acute SARS-CoV-2 infections with each sample sequenced in duplicate. They use these data to characterize the within-host mutational patterns and diversity and relate them to SARS-CoV-2 diversity in consensus sequences sampled around the globe. It further allows understanding how this variation can or cannot be used to understand transmission dynamics and other applications in genomic epidemiology.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife, ScreenIT

2. The role of ventromedial prefrontal cortex in reward valuation and future thinking during intertemporal choice

   This article has 10 authors:

   1. Elisa Ciaramelli
   2. Flavia De Luca
   3. Donna Kwan
   4. Jenkin Mok
   5. Francesca Bianconi
   6. Violetta Knyagnytska
   7. Carl Craver
   8. Leonard Green
   9. Joel Myerson
   10. R Shayna Rosenbaum

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper provides important data on the contribution of the vmPFC to temporal discounting, by showing differential modulation of two well-known effects in inter-temporal choice (magnitude effect, episodic future thinking). VMPFC damage abolished the magnitude effect, but not a modulation of discounting via future thinking, a finding of interest to cognitive neuroscientists working on prospection, decision-making and executive control.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

3. Pathogen invasion-dependent tissue reservoirs and plasmid-encoded antibiotic degradation boost plasmid spread in the gut

   This article has 12 authors:

   1. Erik Bakkeren
   2. Joana Anuschka Herter
   3. Jana Sanne Huisman
   4. Yves Steiger
   5. Ersin Gül
   6. Joshua Patrick Mark Newson
   7. Alexander Oliver Brachmann
   8. Jörn Piel
   9. Roland Regoes
   10. Sebastian Bonhoeffer
   11. Médéric Diard
   12. Wolf-Dietrich Hardt

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work describes an important feature of within-host acquisition of antibiotic resistance. This is a follow-up to their recent publication (Bakkeren et al. 2019), and complements their finding of persister cells in the tissues, to show that also chronic, tissue residing bacteria can provide plasmid tissue reservoirs. The experiments the authors performed are elegant and timely. This manuscript will be of interest to readers in the fields of infection biology, plasmid ecology, gut microbiomes, and antimicrobial resistance.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite

   This article has 17 authors:

   1. Meret Huber
   2. Thomas Roder
   3. Sandra Irmisch
   4. Alexander Riedel
   5. Saskia Gablenz
   6. Julia Fricke
   7. Peter Rahfeld
   8. Michael Reichelt
   9. Christian Paetz
   10. Nicole Liechti
   11. Lingfei Hu
   12. Zoe Bont
   13. Ye Meng
   14. Wei Huang
   15. Christelle AM Robert
   16. Jonathan Gershenzon
   17. Matthias Erb

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to a broad audience interested in the coevolutionary arms race between plants and their herbivores. In a thoroughly investigated case study, the detoxification strategy of cockchafer larvae towards the major defensive compound of one of their preferred host plants, dandelion, is revealed and effects on the behavior of the larvae are described.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

5. Cell-to-cell heterogeneity in Sox2 and Bra expression guides progenitor motility and destiny

   This article has 10 authors:

   1. Michèle Romanos
   2. Guillaume Allio
   3. Myriam Roussigné
   4. Léa Combres
   5. Nathalie Escalas
   6. Cathy Soula
   7. François Médevielle
   8. Benjamin Steventon
   9. Ariane Trescases
   10. Bertrand Bénazéraf

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study is of potential interest to biologists interested in developmental patterning and the link between cellular identity and behavior. The authors perform experiments in the progenitor zone of avian embryos to propose that heterogeneity of cellular behaviors may drive morphogenesis and underlie cell fate choices. The work is nicely done but might need some additional experimental validation of the proposed hypothesis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

6. A proteome-wide genetic investigation identifies several SARS-CoV-2-exploited host targets of clinical relevance

   This article has 18 authors:

   1. Mohd Anisul
   2. Jarrod Shilts
   3. Jeremy Schwartzentruber
   4. James Hayhurst
   5. Annalisa Buniello
   6. Elmutaz Shaikho Elhaj Mohammed
   7. Jie Zheng
   8. Michael Holmes
   9. David Ochoa
   10. Miguel Carmona
   11. Joseph Maranville
   12. Tom R Gaunt
   13. Valur Emilsson
   14. Vilmundur Gudnason
   15. Ellen M McDonagh
   16. Gavin J Wright
   17. Maya Ghoussaini
   18. Ian Dunham

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study analyzes publicly available genomic and proteomic data to identify host proteins that may be involved in regulation of COVID-19 susceptibility and severity. Several known and new targets are identified, further revealing the complexity of host genetic variation in COVID-19 disease.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife, ScreenIT

7. Activation of mTORC1 and c-Jun by Prohibitin1 loss in Schwann cells may link mitochondrial dysfunction to demyelination

   This article has 8 authors:

   1. Gustavo Della-Flora Nunes
   2. Emma R Wilson
   3. Edward Hurley
   4. Bin He
   5. Bert W O'Malley
   6. Yannick Poitelon
   7. Lawrence Wrabetz
   8. M Laura Feltri

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript builds upon the recent observation that Schwann cell (SC)-specific loss of the mitochondrial protein Prohibitin-1 results in a rapid, progressive demyelinating peripheral neuropathy in mice associated with mitochondrial dysfunction. They establish pathways as downstream effectors of mitochondrial dysfunction in Schwann cells. The authors provide a comprehensive evaluation of these pathways following the loss of Prophibitin-1 and identify JUN and mTORC1 as potential mediators of myelin disruption.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

8. Gjd2b-mediated gap junctions promote glutamatergic synapse formation and dendritic elaboration in Purkinje neurons

   This article has 7 authors:

   1. Sahana Sitaraman
   2. Gnaneshwar Yadav
   3. Vandana Agarwal
   4. Shaista Jabeen
   5. Shivangi Verma
   6. Meha Jadhav
   7. Vatsala Thirumalai

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Sitaraman and colleagues address the fundamental question of whether gap junctions facilitate the formation of chemical synapses. To do so they test the function of the gap junction protein, Gjd2b, in early stages of synaptogenesis in larval Zebrafish cerebellar Purkinje neurons. They provide convincing evidence that Gjd2b is necessary for the development of glutamatergic synapses and dendritic arbor growth Purkinje neurons in vivo and that CaMKII plays a role in regulating arbor development. This study will be an important contribution to our understanding of molecular and cellular mechanisms underlying brain development.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

9. Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner

   This article has 4 authors:

   1. Sun-Hee Hwang
   2. Bandarigoda N Somatilaka
   3. Kevin White
   4. Saikat Mukhopadhyay

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to scientists interested in the biology of the G-protein-coupled receptor Gpr161 and its ciliary regulation of Hedgehog (Hh) signaling. The phenotypes observed in a new GPR161 mutant mouse carrying a hypomorphic allele provide additional information showing which developing tissues are more sensitive to Gpr161 function. However, the data at this stage are insufficient to support the main novel conclusion: the ciliary function of Gpr161 is to regulate Gli3 repressor while the extra-ciliary function is to regulate Gli activator.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

10. Subcellular proteomics of dopamine neurons in the mouse brain

    This article has 6 authors:

    1. Benjamin D Hobson
    2. Se Joon Choi
    3. Eugene V Mosharov
    4. Rajesh K Soni
    5. David Sulzer
    6. Peter A Sims

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      In this work, the authors provide a useful compendium of proteins labeled within SNpc dopaminergic cells using a novel approach. AAV virus was used to conditional express the APEX2 enzyme in dopaminergic neurons (based on DAT-1 Cre genetic technology) to rapidly biotinylate nearby proteins in oriented sections of brain whereby the striatonigral circuit can be spatially parsed for proteomic dissection. In addition to providing a useful new database of proteins for investigators interested in this circuit, the results also provide a more general approach to examining a compartment proteome in neurons and what might be expected in that analysis.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife