eLife

Latest preprint reviews

1. Intrinsic mechanisms in the gating of resurgent Na+ currents

   This article has 5 authors:

   1. Joseph L Ransdell
   2. Jonathan D Moreno
   3. Druv Bhagavan
   4. Jonathan R Silva
   5. Jeanne M Nerbonne

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     After more than 20 years of intensive research the molecular machinery of Resurgent Currents (INaR), a non-canonical identity of currents mediated by voltage-activated sodium channels is still a mystery. In this paper, Ransdell and colleagues advance the conceptual framework with new experimental insight and a new kinetic model of INaR.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

2. Transcriptomic and genetic analyses identify the Krüppel-like factor dar1 as a master regulator of tube-shaped long tendon development

   This article has 4 authors:

   1. Laurichesse Quentin
   2. Moucaud Blandine
   3. Jagla Krzysztof
   4. Soler Cédric

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The paper is of interest for those studying tendon development. Starting from a transcriptomic analysis of Drosophila leg tendon cells it identifies a transcription factor, Dar1, required for normal tendon morphogenesis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. HspB8 prevents aberrant phase transitions of FUS by chaperoning its folded RNA-binding domain

   This article has 19 authors:

   1. Edgar E Boczek
   2. Julius Fürsch
   3. Marie Laura Niedermeier
   4. Louise Jawerth
   5. Marcus Jahnel
   6. Martine Ruer-Gruß
   7. Kai-Michael Kammer
   8. Peter Heid
   9. Laura Mediani
   10. Jie Wang
   11. Xiao Yan
   12. Andrej Pozniakovski
   13. Ina Poser
   14. Daniel Mateju
   15. Lars Hubatsch
   16. Serena Carra
   17. Simon Alberti
   18. Anthony A Hyman
   19. Florian Stengel

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work traces at the protein domain level the associations made within droplets containing RNA-binding protein FUS and how they change as a function of time (and maturity), in the presence and absence of the small heat-shock protein HSPB8, by chemical cross-linking coupled to mass spectrometry. This work is an important step forward in our general understanding of the macromolecular interactions within liquid-liquid phase-separated protein droplets, and how they are regulated by small heat-shock protein molecular chaperones.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, #2, and #3 agreed to shares their name with the authors.)

   Reviewed by eLife

4. TMEM120A contains a specific coenzyme A-binding site and might not mediate poking- or stretch-induced channel activities in cells

   This article has 10 authors:

   1. Yao Rong
   2. Jinghui Jiang
   3. Yiwei Gao
   4. Jianli Guo
   5. Danfeng Song
   6. Wenhao Liu
   7. Mingmin Zhang
   8. Yan Zhao
   9. Bailong Xiao
   10. Zhenfeng Liu

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The paper describes novel structures of a protein recently reported to function as a mechanosensitive ion channel. Surprisingly, the structures and functional data rather support the formerly suggested role of this protein in lipid metabolism. The paper is of relevance for ion channel field and for those interested in fatty acid metabolism.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

5. Experimental evidence for delayed post-conflict management behaviour in wild dwarf mongooses

   This article has 3 authors:

   1. Amy Morris-Drake
   2. Julie M Kern
   3. Andrew N Radford

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This article will be of interest to behavioural ecologists studying aggression, within-group conflict, communication, and the use of social information. The study elegantly combines well-designed experiments with field observations to investigate the effects of within-group conflict on social behaviour. Specifically, it expands our understanding of social dynamics in group-living species by providing evidence that bystanders of within-group conflict may play a role in maintaining group cohesion. The findings provide a valuable contribution, and contrast, to existing work in this field.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

6. Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosis

   This article has 29 authors:

   1. Christopher J Brereton
   2. Liudi Yao
   3. Elizabeth R Davies
   4. Yilu Zhou
   5. Milica Vukmirovic
   6. Joseph A Bell
   7. Siyuan Wang
   8. Robert A Ridley
   9. Lareb SN Dean
   10. Orestis G Andriotis
   11. Franco Conforti
   12. Lennart Brewitz
   13. Soran Mohammed
   14. Timothy Wallis
   15. Ali Tavassoli
   16. Rob M Ewing
   17. Aiman Alzetani
   18. Benjamin G Marshall
   19. Sophie V Fletcher
   20. Philipp J Thurner
   21. Aurelie Fabre
   22. Naftali Kaminski
   23. Luca Richeldi
   24. Atul Bhaskar
   25. Christopher J Schofield
   26. Matthew Loxham
   27. Donna E Davies
   28. Yihua Wang
   29. Mark G Jones

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study builds upon the previous original work of the authors by identifying a pathway that regulates collagen nanostructure and stiffness in lung fibrosis and demonstrating that this pathway it is independent of pathways regulating collagen synthesis. The analysis performed to validate the specificity of experimental findings and the demonstration that HIF activation is required for the increased tissue stiffness associated with fibrosis are elegant and convincing. With some additional clarifications and controls, this manuscript will be of broad interest to researchers interested in lung biology and especially to those focused on the pathogenesis and therapy of lung fibrosis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

7. Role of BRCA2 DNA-binding and C-terminal domain in its mobility and conformation in DNA repair

   This article has 8 authors:

   1. Maarten W Paul
   2. Arshdeep Sidhu
   3. Yongxin Liang
   4. Sarah E van Rossum-Fikkert
   5. Hanny Odijk
   6. Alex N Zelensky
   7. Roland Kanaar
   8. Claire Wyman

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work is of interest to readers in the field of genome stability, DNA repair and associated human diseases. The manuscript describes systematic analyses of the crucial DNA repair mediator BRCA2 and its variants lacking the DNA binding domain or RAD51 interacting C-terminal domain, and the conclusions present a conceptual advance as to how BRCA2 promotes DNA repair. The work is a technical tour de force that includes evaluation of the DNA damage response, gene targeting and single particle tracking in mouse embryonic stem cells, as well as biophysical analyses of the human counterparts. The key claims of the manuscript are largely supported by the data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

8. Ectocytosis prevents accumulation of ciliary cargo in C. elegans sensory neurons

   This article has 2 authors:

   1. Adria Razzauti
   2. Patrick Laurent

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Razzauti and Laurent investigate the formation of extracellular vesicles (EVs) by the cilia of C. elegans sensory neurons and the potential functions of this process. Consistent with previous findings, they show that EVs can be released from two distinct sites of the cilium; further, they show that several different classes of sensory neurons can produce EVs, that these can be taken up by a neighboring glial cell, and that this process may be important for the morphology and function of ciliated sensory neurons. However, it remains unclear whether these phenomena may be a consequence of the experimental system (ciliary protein overexpression); additionally, the link between EV uptake by glia and maintenance of neuronal structure and function is not convincingly established.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

9. Reversal of the adipostat control of torpor during migration in hummingbirds

   This article has 3 authors:

   1. Erich R Eberts
   2. Christopher G Guglielmo
   3. Kenneth C Welch

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study substantially advances our understanding of why, when, and how wild birds use daily torpor as an energetic strategy. A technically challenging combination of carefully controlled experiments on temporarily captive wild birds along with frequent respiratory measurements and whole-body composition measurements was key to making this advance.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. Processing of the ribosomal ubiquitin-like fusion protein FUBI-eS30/FAU is required for 40S maturation and depends on USP36

    This article has 7 authors:

    1. Jasmin van den Heuvel
    2. Caroline Ashiono
    3. Ludovic C Gillet
    4. Kerstin Dörner
    5. Emanuel Wyler
    6. Ivo Zemp
    7. Ulrike Kutay

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This study has discovered deubiquitinase USP36 as the enzyme that processes FAU, a ribosomal protein precursor comprised of a fusion between ubiquitin-like protein FUBI and the ribosomal protein eS30. This is an important advance because correct processing is crucial for biogenesis of the 40S ribosomal subunit. Knowing the identity of the processing enzyme now opens this step in ribosome biogenesis to molecular and mechanistic analysis.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife