eLife

Latest preprint reviews

1. RAB14-dependent tubulovesicular recycling directs MET to invadopodia, promoting TNBC cell invasion

   This article has 6 authors:

   1. Amrita Khamari
   2. Atreyee Guria
   3. Kiran Tak
   4. Rajiv Sharma
   5. Yannis Kalaidzidis
   6. Sunando Datta

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This revised paper provides solid evidence for HGF-induced trafficking of the HGF receptor, MET, together with metalloprotease MT1-MMP into invadopodia and the role of this trafficking in triple-negative breast cancer (TNBC) cell invasion in vitro. The evidence could be improved with additional experimental controls and analysis, but the findings will be valuable for cancer cell biologists investigating TNBC.

   Reviewed by eLife

2. We couldn't get details of this article at this time:
   10.1101/2025.08.24.672027

3. Efficient transduction of pancreas tissue slices with genetically encoded calcium integrators

   This article has 9 authors:

   1. Charles S Lazimi
   2. Austin E Stis
   3. Julia K Panzer
   4. Helmut Hiller
   5. Maria L Beery
   6. Amelia K Linnemann
   7. Cherie L Stabler
   8. Clayton E Mathews
   9. Edward A Phelps

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a valuable advance by enabling functional mapping of Ca²⁺ responses in live human pancreatic tissue slices, providing new opportunities to study islet heterogeneity and diabetes-related dysfunction in an intact tissue context. The evidence supporting the main conclusions is solid, based on reproducible methodology and functional validation across multiple human donor samples. Key revisions needed include clearer quantification of transduction efficiency and tissue viability, and improved clarification of how CaMPARI2 signals should be interpreted.

   Reviewed by eLife

4. Rapid evolution of fine-scale recombination during domestication: a perspective from population genomics

   This article has 17 authors:

   1. Zheng-Xi Liu
   2. Ming Li
   3. Xue-Hai Ge
   4. Kun Wang
   5. Si Si
   6. Chang-Rong Ge
   7. Jian-Hai Chen
   8. Li-Rong Hu
   9. Min-Sheng Peng
   10. Ting-Ting Yin
   11. Ali Esmailizadeh
   12. Chang Zhang
   13. Lu-Jiang Qu
   14. Xue-Mei Lu
   15. Jian-Lin Han
   16. Ya-Ping Zhang
   17. Ming-Shan Wang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This paper addresses valuable questions about the evolution of recombination landscape under domestication by examining recombination maps in domesticated chickens and their wild ancestor. However, despite employing a state-of-the-art deep learning method for recombination map inference, the lack of systematic benchmarking and presence of some unexpected patterns raise concerns about the reliability of the inferred maps, thus providing incomplete support for rapid evolution of recombination landscapes. Additionally, due to methodological limitations in testing for intra-genome correlations between evolutionary processes, the current evidence is inadequate to support the associations of recombination with selection and/or introgression in domesticated chickens.

   Reviewed by eLife

5. We couldn't get details of this article at this time:
   10.1101/2025.08.28.672825

6. Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching

   This article has 3 authors:

   1. Kexin Zhang
   2. Timothy Grant
   3. Nikolaus Grigorieff

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study builds on previous work from the same authors to present a conceptually distinct workflow for cryo-EM reconstruction that uses 2D template matching to enable high-resolution structure determination of small (sub-50 kDa) protein targets. The paper provides convincing evidence that the density for small-molecule ligands bound to such targets can be reconstructed without these ligands being present in the template, and it provides an insightful quantification of the level of model bias that remains in the resulting reconstructions. The convenient visualisation of small molecules bound to protein targets of a known structure will be relevant for the pharmaceutical industry.

   Reviewed by eLife

7. Reproducibility of Scientific Claims in Drosophila Immunity: A Retrospective Analysis of 400 Publications

   This article has 30 authors:

   1. Hannah Westlake
   2. Fabrice David
   3. Yao Tian
   4. Kenan Krakovic
   5. Asya Dolgikh
   6. Liza Juravlev
   7. Thomas Esmangart de Bournonville
   8. Alexia Carboni
   9. Claudia Melcarne
   10. Tisheng Shan
   11. Yang Wang
   12. Yizhu Mu
   13. Akshata Kotwal
   14. Nadia Pirko
   15. Jean Philippe Boquete
   16. Fanny Schüpfer
   17. Samuel Rommelaere
   18. Mickael Poidevin
   19. Zhonggeng Liu
   20. Shu Kondo
   21. Girish S Ratnaparkhi
   22. Sveta Chakrabarti
   23. Guiqing Liu
   24. Florent Masson
   25. Li Xiaoxue
   26. Mark A Hanson
   27. Haobo Jiang
   28. Francesca Di Cara
   29. Estee Kurant
   30. Bruno Lemaitre

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This fundamental study is part of an impressive, large-scale effort to assess the reproducibility of published findings in the field of Drosophila immunity. In a companion article, the authors analyze 400 papers published between 1959 and 2011, and assess how many of the claims in these papers have been tested in subsequent publications. In this article, the authors report the results of validation experiments to assess a subset of the claims that, according to the literature, have not been corroborated. While the evidence reported for some of these validation studies is convincing, it remains incomplete for others.

   Reviewed by eLife

8. We couldn't get details of this article at this time:
   10.1101/2025.08.11.669642

9. Dual-feature selectivity enables bidirectional coding in visual cortical neurons

   This article has 19 authors:

   1. Nikos Karantzas
   2. Katrin Franke
   3. Konstantin Willeke
   4. Maria Diamantaki
   5. Kandan Ramakrishnan
   6. Hasan Atakan Bedel
   7. Pavithra Elumalai
   8. Kelli Restivo
   9. Paul Fahey
   10. Cate Nealley
   11. Tori Shinn
   12. Gabrielle Garcia
   13. Saumil Patel
   14. Alexander Ecker
   15. Edgar Y Walker
   16. Emmanouil Froudarakis
   17. Sophia Sanborn
   18. Fabian H Sinz
   19. Andreas Tolias

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors combine a modeling approach, using a digital twin, with electrophysiological evidence in two species to assess the role of inhibition in shaping selectivity in the visual cortex. The results provide a fundamental advance beyond the classic view of sensory coding by proving compelling evidence that many neurons in visual areas exhibit dual-feature selectivity. Overall, the work compellingly showcases how in silico experiments can generate concrete hypotheses about neuronal coding that are difficult to discover experimentally.

   Reviewed by eLife

10. A novel high-throughput single-cell DNA sequencing method reveals hidden genomic heterogeneity in the unicellular eukaryote Leishmania

    This article has 4 authors:

    1. Gabriel H Negreira
    2. Pieter Monsieurs
    3. Jean-Claude Dujardin
    4. Malgorzata A Domagalska

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This important study, which tackles the challenge of analyzing genome integrity and instability in unicellular pathogens by introducing a novel single-cell genomics approach, presents compelling evidence that this new tool outperforms standard whole-genome amplification techniques. While thorough and rigorous, the work's impact would increase by providing scripts and data, as well as a description of the biological relevance that would make this method more appealing to the broad community studying genetic heterogeneity in diverse organisms.

    Reviewed by eLife