eLife

Latest preprint reviews

1. Uev1A counteracts oncogenic Ras stimuli in both polyploid and diploid cells

   This article has 13 authors:

   1. Qi Zhang
   2. Yunfeng Wang
   3. Xueli Fu
   4. Ziguang Wang
   5. Yang Zhang
   6. Lizhong Yan
   7. Yuejia Wang
   8. Muhan Yang
   9. Dongze Song
   10. Ruixing Zhang
   11. Hongru Zhang
   12. Shian Wu
   13. Shaowei Zhao

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study examines the role of E2 ubiquitin enzyme, Uev1a in tissue resistance to oncogenic RasV12 in Drosophila melanogaster polyploid germline cells and human cancer cell lines. The incomplete evidence suggests that Uev1a works with the E3 ligase APC/C to degrade Cyclin A, and the strength of evidence could be increased by addressing the expression of CycA in the ovaries and the uev1a loss of function in human cancer cells. This work would be of interest to researchers in germline biology and cancer.

   Reviewed by eLife

2. Structure of an oxygen-induced tubular nanocompartment in Pyrococcus furiosus

   This article has 11 authors:

   1. Wenfei Song
   2. Jan Fiala
   3. Ioannis Skalidis
   4. Pascal Albanese
   5. Constantinos Patinios
   6. Marten L Chaillet
   7. Servé WM Kengen
   8. Richard A Scheltema
   9. Stuart C Howes
   10. Albert JR Heck
   11. Friedrich Förster

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Using advanced CryoEM and mass spectrometry, the authors provide compelling evidence of how tubule formation occurs in an oxygen-dependent manner. These fundamental findings offer a novel mechanism by which rubrerythrin tubules encapsulate encapsulin to prevent oxidative stress in Pyrococcus furiosus. However, there are a few reasonable concerns about biochemical validations and the lack of adequate description of results and methodology.

   Reviewed by eLife

3. The denitrosylase SCoR2 controls cardioprotective metabolic reprogramming

   This article has 12 authors:

   1. Zachary W Grimmett
   2. Rongli Zhang
   3. Hua-Lin Zhou
   4. Qiuying Chen
   5. Dawson Miller
   6. Zhaoxia Qian
   7. Justin Lin
   8. Riti Kalra
   9. Steven S Gross
   10. Walter J Koch
   11. Richard T Premont
   12. Jonathan S Stamler

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides new and interesting findings that SCoR2 acts as a denitrosylase to control cardioprotective metabolic reprogramming and prevent injury following ischemia/reperfusion. The compelling evidence is supported by a novel multi-omics approach, but questions remain regarding the stability and human relevance of BDH1 as well as the sufficiency of SCoR2. Overall, the work will be of interest to cardiovascular researchers and provides valuable information to the field, though some mechanistic aspects require further clarification.

   Reviewed by eLife

4. SVEP1 enables efficient binding of Angiopoietin-2 to the TIE1 receptor, allowing receptor phosphorylation and downstream signaling

   This article has 9 authors:

   1. Katharina Uphoff
   2. Melina Hußmann
   3. Dörte Schulte-Ostermann
   4. Yvonne Huisman
   5. Matthias Mörgelin
   6. Fabian Metzen
   7. J Fernando Bazan
   8. Manuel Koch
   9. Stefan Schulte-Merker

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript focuses on developing a structural model of how the multidomain ECM protein SVEP1 enables Angiopoietin (ANG) binding to the orphan receptor TIE1, resulting in downstream receptor phosphorylation and signaling. This is a potentially important study, however, it currently lacks key controls and is therefore incomplete. The data will be of interest to scientists working in vascular biology and RTK signaling.

   Reviewed by eLife

5. High-throughput neutralization measurements correlate strongly with evolutionary success of human influenza strains

   This article has 13 authors:

   1. Caroline Kikawa
   2. Andrea N Loes
   3. John Huddleston
   4. Marlin D Figgins
   5. Philippa Steinberg
   6. Tachianna Griffiths
   7. Elizabeth M Drapeau
   8. Heidi Peck
   9. Ian G Barr
   10. Janet A Englund
   11. Scott E Hensley
   12. Trevor Bedford
   13. Jesse D Bloom

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This fundamental study advances our understanding of population-level immune responses to influenza in both children and adults. The strength of the evidence supporting the conclusions is compelling, with high-throughput profiling assays and mathematical modeling. The work will be of interest to immunologists, virologists, vaccine developers, and those working on mathematical modeling of infectious diseases.

   Reviewed by eLife

6. Effects of Arginine Vasopressin on Islet Cells in Pancreatic Tissue Slices: Glucose-Dependent Modulation of IP3 Receptor-Specific Responses

   This article has 14 authors:

   1. Jasmina Kerčmar
   2. Nastja Murko
   3. Lidija Križančić Bombek
   4. Eva Paradiž Leitgeb
   5. Johannes Pfabe
   6. Sandra Postić
   7. Ya-Chi Huang
   8. Andraž Stožer
   9. Dean Korošak
   10. Xaver Kozisek
   11. Monika Perisic
   12. Markus Muttenthaler
   13. Christian W Gruber
   14. Marjan Slak Rupnik

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a useful finding on the effects of arginine vasopressin (AVP) on islet cells in pancreatic tissue slices, using technically sophisticated spatio-temporal calcium recordings to confirm that AVP influences α and β cells differently depending on glucose concentrations. While the study’s methods – particularly the calcium imaging techniques and peptide ligand design targeting V1b receptors – are strong, the reviewers were concerned about several aspects of the experimental design. The results on β-cell responses are incomplete and insufficient to support the manuscript’s claims, especially due to the high variability of islet responses and lack of mechanistic and functional (hormone release) data. There are also concerns about the possibility of off-target effects and suboptimal receptor specificity: the study would be significantly strengthened by inclusion of signaling pathway interrogation, hormone output assays, genetic validation (e.g., β cell-specific deletion of V1br), and receptor localization. The work will still be of interest to researchers studying islet physiology in the context of health and diabetes.

   Reviewed by eLife

7. Identifying a novel mechanism of L-leucine uptake in Mycobacterium tuberculosis using a chemical genomic approach

   This article has 8 authors:

   1. Nisheeth Agarwal
   2. Himanshu Gogoi
   3. Eeba
   4. Linus Augustin
   5. Md. Younus Khan
   6. Yashwant Kumar
   7. Sayan Kumar Bhowmick
   8. Bappaditya Dey

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     By performing a chemical screen of an FDA-approved library of small molecules against a leucine-dependent Mtb strain, this work discovered that semapimod inhibits Mtb growth by impairing leucine import. The work is useful because it connects leucine uptake with the cell wall lipids in Mtb; however, it remains incomplete as the evidence supporting semapimod's ability to target leucine uptake needs more substantial proof. The work requires significant experimental evidence to identify leucine transporter(s) and determine how PDIM participates in leucine uptake.

   Reviewed by eLife

8. Temperature-based MHC class-I multimer peptide exchange for human HLA-A, B and C

   This article has 13 authors:

   1. Cilia R Pothast
   2. Ian Derksen
   3. Anneloes van der Plas - van Duijn
   4. Angela el Hebieshy
   5. Wesley Huisman
   6. Kees LMC Franken
   7. Jacques Neefjes
   8. Jolien J Luimstra
   9. Marieke Griffioen
   10. Michel Kester
   11. Maarten H Vermeer
   12. Mirjam HM Heemskerk
   13. Ferenc A Scheeren

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents an alternative to conventional and UV-based tetramers, which are easy to use and reliable for the identification of antigen-specific CD8 T cells. The authors demonstrate that tetramers for HLA alleles A0301, A1101, B0702, and C0702 can be subjected to specific temperatures that facilitate peptide exchange, whilst maintaining structural integrity. Whilst the strength of the evidence is currently incomplete, further development and validation of this approach is likely to provide a useful alternative to generating reagents for examining T cell specificities.

   Reviewed by eLife

9. Type I interferon responses contribute to immune protection against mycobacterial infection

   This article has 14 authors:

   1. Andrea Szydlo-Shein
   2. Blanca Sanz-Magallón Duque de Estrada
   3. Joshua Rosenheim
   4. Carolin T Turner
   5. Aneesh Chandran
   6. Evdokia Tsaliki
   7. Marc CI Lipman
   8. Heinke Kunst
   9. Gabriele Pollara
   10. Philip M Elks
   11. Jean-Pierre Levraud
   12. Elspeth M Payne
   13. Mahdad Noursadeghi
   14. Gillian S Tomlinson

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful study shows a protective role of type 1 IFN during Mycobacterium tuberculosis infection. It shows that the type 1 IFN response in human skin TST inversely correlates with TB severity, suggesting its protective role. Considering that type I IFN is usually shown to be pro-pathogenic, the higher vulnerability of zebrafish larvae lacking stat2 to M marinum infection is a strong result. However, the conclusion that IFN-I is protective during mycobacterial infection remains indirect and incomplete; the study requires additional mechanistic insights and validation.

   Reviewed by eLife

10. Synonymous mutations in AAV Rep enhance genome packaging in a library selection

    This article has 3 authors:

    1. Tasfia Azim
    2. Dru Myerscough
    3. Jonathan J Silberg

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      Engineering of adeno-associated virus (AAV) replication proteins may provide new insights into Parvoviral replication. The authors created a useful collection of Rep protein variants with changes that alter the amino acid sequence, but these did not lead to clear improvements in how the virus worked. Instead, their screen showed that changes that do not alter the protein ("synonymous" mutations) and changes to the promoter were more common. As it stands the results are incomplete due to potential issues with the screening design. We encourage a more complete characterization, which may enhance the translational potential of the approach.

    Reviewed by eLife