eLife

Latest preprint reviews

1. Genetic variation in ALDH4A1 is associated with muscle health over the lifespan and across species

   This article has 6 authors:

   1. Osvaldo Villa
   2. Nicole L Stuhr
   3. Chia-an Yen
   4. Eileen M Crimmins
   5. Thalida Em Arpawong
   6. Sean P Curran

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to researchers studying muscle function in the nematode C. elegans and to researchers interested in muscle aging in humans. The work documents the importance of C. elegans alh-6, which encodes a proline catabolic gene, in limiting muscle stress and maintaining locomotory function in aging nematodes. Further work implicates GWAS SNPs in the human homolog ALDH4A1 as potential determinants of specific muscle decline indicators. The suggestion of a conserved role for ALH-6 in aging humans could underlie establishment of a useful biomarker for older age muscle-associated health. However, while the work expands on and reinforces findings in C. elegans, it does not yet rigorously demonstrate a role for ALDH4A1 in muscle function in aging humans.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

2. Diversification of multipotential postmitotic mouse retinal ganglion cell precursors into discrete types

   This article has 5 authors:

   1. Karthik Shekhar
   2. Irene E Whitney
   3. Salwan Butrus
   4. Yi-Rong Peng
   5. Joshua R Sanes

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study using single cell RNA Seq to profile developing retinal ganglion cells from embryonic and postnatal mouse retina explores the diversification of this class of neurons into specific subtypes. The computational approaches developed identify groups of RGC precursors with largely non-overlapping fates, distinguished by selectively expressed transcription factors that could act as fate determinants. The aim is to show that over time, clusters of cells become "decoupled" as they split into subclusters, and this process is associated with changes in the expression of specific transcription factors. This leads to the proposal that subtype diversification arises as a gradual, asynchronous fate restriction of postmitotic multipotential precursors. These findings enable the prediction of lineage relationships among RGC subtypes and the developmental time when these specification events occur, and should be of great interest to the developmental neurobiology community.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. Early life infection and proinflammatory, atherogenic metabolomic and lipidomic profiles in infancy: a population-based cohort study

   This article has 16 authors:

   1. Toby Mansell
   2. Richard Saffery
   3. Satvika Burugupalli
   4. Anne-Louise Ponsonby
   5. Mimi LK Tang
   6. Martin O'Hely
   7. Siroon Bekkering
   8. Adam Alexander T Smith
   9. Rebecca Rowland
   10. Sarath Ranganathan
   11. Peter D Sly
   12. Peter Vuillermin
   13. Fiona Collier
   14. Peter Meikle
   15. David Burgner
   16. Barwon Infant Study Investigator Group

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to a broad audience of epidemiologists and early childhood researchers as it provides data on the relationship between infection burden in infancy and metabolomic/lipidomic profiles at 12 months of age from a unique cohort of 555 mother-infant dyads. The paper also examines potential biologic pathways that may inform prevention of cardiovascular disease. The series of analyses presented support the preliminary associations outlined and require validation and interventional studies to support the causal relationship between infection, cumulative inflammation burden, and atherosclerosis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

4. Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections

   This article has 8 authors:

   1. Marissa Jeme Andersen
   2. ChunKi Fong
   3. Alyssa Ann La Bella
   4. Jonathan Jesus Molina
   5. Alex Molesan
   6. Matthew M Champion
   7. Caitlin Howell
   8. Ana L Flores-Mireles

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In a set of in vitro and in vivo experiments the investigators demonstrated that coating of urinary tract catheters with fibrinogen-degrading substances reduced adhesion and colonization with a broad range of bacteria relevant in the pathogenesis of CAUTI. This approach might, therefore, be interesting for prevention of CAUTI as an alternative to catheters coated with antibiotics.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

5. Investigating phenotypes of pulmonary COVID-19 recovery: A longitudinal observational prospective multicenter trial

   This article has 20 authors:

   1. Thomas Sonnweber
   2. Piotr Tymoszuk
   3. Sabina Sahanic
   4. Anna Boehm
   5. Alex Pizzini
   6. Anna Luger
   7. Christoph Schwabl
   8. Manfred Nairz
   9. Philipp Grubwieser
   10. Katharina Kurz
   11. Sabine Koppelstätter
   12. Magdalena Aichner
   13. Bernhard Puchner
   14. Alexander Egger
   15. Gregor Hoermann
   16. Ewald Wöll
   17. Günter Weiss
   18. Gerlig Widmann
   19. Ivan Tancevski
   20. Judith Löffler-Ragg

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript which links early markers of inflammation with residual abnormalities on chest CT following SARS-CoV-2 infection. Surprisingly, early surveyed symptoms do not predict long term radiologic outcomes (6 months after infection) while inflammatory markers have stronger predictive value. Residual symptoms are common at the 6 month time point.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife, ScreenIT

6. Progressive enhancement of kinetic proofreading in T cell antigen discrimination from receptor activation to DAG generation

   This article has 3 authors:

   1. Derek M Britain
   2. Jason P Town
   3. Orion David Weiner

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this manuscript, a light-gated receptor system (LOV2) linked to T cell receptor signaling machinery is enhanced by addition of an adhesion system enabling robust operation over a larger range of kinetic parameters. This system enables an exploration of how kinetic proofreading processes executed in seconds relate to T cell activation program involving reactions taking minutes to hours.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Deep-sequence phylogenetics to quantify patterns of HIV transmission in the context of a universal testing and treatment trial – BCPP/Ya Tsie trial

   This article has 18 authors:

   1. Lerato E Magosi
   2. Yinfeng Zhang
   3. Tanya Golubchik
   4. Victor DeGruttola
   5. Eric Tchetgen Tchetgen
   6. Vladimir Novitsky
   7. Janet Moore
   8. Pam Bachanas
   9. Tebogo Segolodi
   10. Refeletswe Lebelonyane
   11. Molly Pretorius Holme
   12. Sikhulile Moyo
   13. Joseph Makhema
   14. Shahin Lockman
   15. Christophe Fraser
   16. Myron Max Essex
   17. Marc Lipsitch
   18. On behalf of The Botswana Combination Prevention Project and PANGEA consortium

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     One hypothesis to explain recent worse-than-expected outcomes of universal test-and-treat HIV prevention trials is population mobility. The authors show phylogenetically that mobility could play a role in transmission events in a large trial in Botswana. This study is of public health interest, has a large sample size for a phylogenetic study in this setting, and overall precise analysis. A few methodological clarifications are still needed to ensure that the data supports the study's claims.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. First-order visual interneurons distribute distinct contrast and luminance information across ON and OFF pathways to achieve stable behavior

   This article has 6 authors:

   1. Madhura D Ketkar
   2. Burak Gür
   3. Sebastian Molina-Obando
   4. Maria Ioannidou
   5. Carlotta Martelli
   6. Marion Silies

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to neuroscientists studying visual processing and is also more broadly relevant to understanding how sensory systems process information. The paper reveals several new insights into how first-order interneurons in the fly visual system encode visual features that help guide behavior.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

9. Exploring the expression patterns of palmitoylating and de-palmitoylating enzymes in the mouse brain using the curated RNA-seq database BrainPalmSeq

   This article has 8 authors:

   1. Angela R Wild
   2. Peter W Hogg
   3. Stephane Flibotte
   4. Glory G Nasseri
   5. Rocio B Hollman
   6. Danya Abazari
   7. Kurt Haas
   8. Shernaz X Bamji

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of broad interest to neuroscientists, providing a rich resource for future research. Using available RNAseq data the authors build an easy-to-work-with web platform which will enable researchers to survey the expression patterns of palmitoylating and de-palmitoylating enzymes and their potential co-expressed substrates within the mouse nervous system. Using this map, the authors test hypotheses about the relationship between these enzymes and neurological diseases and generate hypotheses about enzyme/substrate relationships based on expression correlations.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. A discrete parasubthalamic nucleus subpopulation plays a critical role in appetite suppression

    This article has 14 authors:

    1. Jessica H Kim
    2. Grace H Kromm
    3. Olivia K Barnhill
    4. Jacob Sperber
    5. Lauren B Heuer
    6. Sierra Loomis
    7. Matthew C Newman
    8. Kenneth Han
    9. Faris F Gulamali
    10. Theresa B Legan
    11. Katharine E Jensen
    12. Samuel C Funderburk
    13. Michael J Krashes
    14. Matthew E Carter

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This paper will be of interest to neuroscientists studying ingestive behavior and control of body weight. It reveals two distinct subsets of neurons within a little-studied brain area that are both activated by feeding, but only of them contributes to hormone-mediated suppression of feeding. The combination of molecular profiling and functional modulation of the neurons compellingly support the claims of the paper.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

    Reviewed by eLife