eLife

Latest preprint reviews

1. Sulfation affects apical extracellular matrix organization during development of the Drosophila embryonic salivary gland tube

   This article has 6 authors:

   1. J Luke Woodward
   2. Jeffrey Matthew
   3. Rutuparna Joshi
   4. Vishakha Vishwakarma
   5. Ying Xiao
   6. SeYeon Chung

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This paper is important in demonstrating a requirement for sulfation in organizing apical extracellular matrix (aECM) during tubulogenesis in Drosophila melanogaster. The authors identify and characterize the organization of some of the first known components of the non-chitinous aECM in the Drosophila salivary gland tube, and these findings are supported by convincing data. This study would be of interest to developmental and cell biologists.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife, Review Commons

2. Evidence of off-target probe binding affecting 10x Genomics Xenium gene panels compromise accuracy of spatial transcriptomic profiling

   This article has 5 authors:

   1. Caleb Hallinan
   2. Hyun Joo Ji
   3. Edmund Tsou
   4. Steven L Salzberg
   5. Jean Fan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study identifies and characterizes probe binding errors in a widely used commercial platform for spatial transcriptomics, discovering that at least 21 out of 280 genes in a human breast cancer panel are not accurately detected. The authors provide convincing evidence for their findings through validation against multiple independent sequencing technologies and reference datasets, and they introduce a computational tool to help predict potential off-target probe binding. Given the broad adoption of this platform in biomedical research, this work provides an essential quality control resource that will improve data interpretation across numerous studies.

   Reviewed by eLife

3. Single-cell transcriptome sequencing for opening the blood-brain barrier through specific mode electroacupuncture stimulation

   This article has 11 authors:

   1. Congcong Ma
   2. Zhaoxing Jia
   3. Tianxiang Jiang
   4. Qian Cai
   5. Jinding Yang
   6. Lin Gan
   7. Kecheng Qian
   8. Zixin Pan
   9. Qinyu Ye
   10. Mengyuan Dai
   11. Xianming Lin

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a valuable finding that the blood-brain barrier (BBB) may be modulated through specific modes of electroacupuncture stimulation. The data were collected and analyzed using a solid and validated methodology, and can be used as a starting point for functional studies of the BBB for drug delivery across healthy and diseased states. The work will be of broad interest to scientists working in the field of drug delivery and drug development.

   Reviewed by eLife

4. The basolateral amygdala complex and perirhinal cortex represent focal and peripheral states of information processing in rats

   This article has 4 authors:

   1. Francesca S Wong
   2. Simon Killcross
   3. R Fred Westbrook
   4. Nathan M Holmes

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important Research Advance builds on the authors' previous work delineating the roles of the rodent perirhinal cortex and the basolateral amygdala in first- and second-order learning. The convincing results show that serial exposure of non-motivationally relevant stimuli influences how those stimuli are encoded within the perirhinal cortex and basolateral amygdala when paired with a shock. This manuscript will be interesting for researchers in cognitive and behavioral neuroscience.

   Reviewed by eLife

5. Peripheral anatomy and central connectivity of proprioceptive sensory neurons in the Drosophila wing

   This article has 3 authors:

   1. Ellen Lesser
   2. Anthony J Moussa
   3. John C Tuthill

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important work describes wing mechanosensory neurons in detail, extending our understanding of sensorimotor processing in the fruit fly. The evidence presented convincingly supports the authors' identification of these neurons and leverages state-of-the-art methods to generate a near-complete map of wing mechanosensory circuitry. Overall, this study provides new hypotheses and invaluable tools for investigating proprioceptive motor control of the wing in Drosophila.

   Reviewed by eLife

6. The hypoxic response extends lifespan through a bioaminergic and peptidergic neural circuit

   This article has 13 authors:

   1. Elizabeth S Kitto
   2. Shijiao Huang
   3. Mira Bhandari
   4. Cassie Tian
   5. Rebecca L Cox
   6. Safa Beydoun
   7. Emily Wang
   8. Danielle Shave
   9. Hillary A Miller
   10. Sarah A Easow
   11. Ella Henry
   12. Megan L Schaller
   13. Scott F Leiser

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This fundamental study identifies specific neural mechanisms through which HIF-1 signaling in ADF serotonergic neurons extends lifespan in C. elegans, revealing that downstream signaling in multiple types of neurons, as well as other neuromodulators like GABA, tyramine, and NLP-17, is required for this effect. The strength of the evidence is largely convincing, as the authors establish the necessity and causality of key neuronal components using multiple genetic tools and functional dissection in a well-validated model organism.

   Reviewed by eLife

7. Comprehensive Neural Representations of Naturalistic Stimuli through Multimodal Deep Learning

   This article has 5 authors:

   1. Mingxue Fu
   2. Guoqiu Chen
   3. Yijie Zhang
   4. Mingzhe Zhang
   5. Yin Wang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a valuable application of a video-text alignment deep neural network model to improve neural encoding of naturalistic stimuli in fMRI. The authors provide convincing evidence that models based on multimodal and dynamic embedding features of audiovisual movies predicted brain responses better than models based on unimodal or static features. The work will be of interest to researchers in cognitive neuroscience and AI-based brain modeling.

   Reviewed by eLife

8. Transcriptional responses to chronic oxidative stress require cholinergic activation of G-protein-coupled receptor signaling

   This article has 11 authors:

   1. Kasturi Biswas
   2. Caroline Moore
   3. Hannah Rogers
   4. Khursheed A Wani
   5. Arjamand Mushtaq
   6. Read Pukkila-Worley
   7. Daniel P Higgins
   8. Amy K Walker
   9. Gregory P Mullen
   10. James B Rand
   11. Michael M Francis

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study advances our understanding of how organisms respond to chronic oxidative stress. Using the nematode C. elegans, the authors identified key neuronal signaling molecules and their receptors that are required for stress signaling and survival. The evidence supporting the conclusions is solid, including rigorous genetics, stress response analysis, and transcriptional profiling. This research will be of broad interest to neuroscientists and researchers working in the field of oxidative stress regulation.

   Reviewed by eLife

9. Trpv4 links environmental temperature to testicular differentiation in hermaphroditic ricefield eel

   This article has 4 authors:

   1. Yang Zhi
   2. Luo Tingting
   3. Zhang Yimin
   4. Sun Yuhua

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents useful findings on the molecular mechanisms driving female-to-male sex reversal in the ricefield eel (Monopterus albus) during aging, which would be of interest to biologists studying sex determination. The manuscript describes an interesting mechanism potentially underlying sex differentiation in M. albus. However, the current data are incomplete and would benefit from more rigorous experimental approaches.

   Reviewed by eLife

10. An expanded palette of bright and photostable organellar Ca2+ sensors

    This article has 12 authors:

    1. Agathe Moret
    2. Helen Farrants
    3. Ruolin Fan
    4. Kelsey G Zingg
    5. Bryon Silva
    6. Camilla Roselli
    7. Thomas G Oertner
    8. Christine E Gee
    9. Dafni Hadjieconomou
    10. Vidhya Rangaraju
    11. Eric R Schreiter
    12. Jaime de Juan-Sanz

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This important study introduces a new class of spectrally tunable, dye-based calcium sensors optimized for imaging in organelles with high calcium concentrations, such as the endoplasmic reticulum and mitochondria. The experimental evidence supporting the applicability of these sensors is convincing, with thorough validation in cultured cells and neurons. The work will be of high interest to researchers studying calcium signaling dynamics in subcellular compartments.

    Reviewed by eLife