eLife

Latest preprint reviews

1. Mechanism of the cadherin–catenin F-actin catch bond interaction

   This article has 3 authors:

   1. Amy Wang
   2. Alexander R Dunn
   3. William I Weis

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work investigated the mechanism of the cadherin-catenin F-actin catch bond interaction, a fundamental cell-cell adhesive structure that can be both dynamic and force-activated. Force measurements with purified protein components demonstrate that the catch bond results from a force-dependent switch of the actin-binding domain of αE-catenin between a five-helix bundle and a four-helix bundle bound on F-actin. The findings are interesting and well supported by experimental data, and will be interesting to the broader field of cytoskeleton function and functional structural biology.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

2. Proteogenomic analysis of cancer aneuploidy and normal tissues reveals divergent modes of gene regulation across cellular pathways

   This article has 13 authors:

   1. Pan Cheng
   2. Xin Zhao
   3. Lizabeth Katsnelson
   4. Elaine M Camacho-Hernandez
   5. Angela Mermerian
   6. Joseph C Mays
   7. Scott M Lippman
   8. Reyna Edith Rosales-Alvarez
   9. Raquel Moya
   10. Jasmine Shwetar
   11. Dominic Grun
   12. David Fenyo
   13. Teresa Davoli

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work by Cheng et al evaluates the contribution of regulation of gene expression at the RNA and protein level by leveraging copy number variations in a large cohort of cancer samples. Importantly they find that there is rarely compensatory regulation at the RNA and protein level together, but depending on the gene, expression is either compensated at one or the other. The paper is very intriguing and the findings are of interest to a broad readership.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

3. Multiple UBX proteins reduce the ubiquitin threshold of the mammalian p97-UFD1-NPL4 unfoldase

   This article has 3 authors:

   1. Ryo Fujisawa
   2. Cristian Polo Rivera
   3. Karim PM Labib

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper describes a biochemical analysis of the roles of Ub chain length on Ub-dependent segregase activity of yeast and human p97 and the role of UBX proteins on the disassembly of the CMG replicative helicase complex. The human p97 complex does not segregate substrates with shorter ubiquitin chains as efficiently as does the yeast complex but the human complex can be enhanced in vitro by 3 UBX proteins - FAF1, FAF2, and UBXN7. Cellular studies indicate a partial role for FAF1 and UBXN7 in cells. The paper would be strengthened by additional mechanistic understanding of how the UBX domain functions in activation of segregase activity and the contribution of this pathway in cells.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. Low and high frequency intracranial neural signals match in the human associative cortex

   This article has 5 authors:

   1. Corentin Jacques
   2. Jacques Jonas
   3. Sophie Colnat-Coulbois
   4. Louis Maillard
   5. Bruno Rossion

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper can provide novel basic science insights into how brain responses at different frequencies signal sensory information. An analysis of intracranial recordings in 121 human participants shows that low-frequency local field potentials and high-frequency (>30 Hz) broadband activity in response to face stimuli have largely similar spatial, functional, and timing properties. However, it is essential to show that the two response types can be reliably disambiguated and to critically discuss the generalizability of the findings.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

5. Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons

   This article has 10 authors:

   1. Jennifer M Lin
   2. Tyler A Mitchell
   3. Megan Rothstein
   4. Alison Pehl
   5. Ed Zandro M Taroc
   6. Raghu R Katreddi
   7. Katherine E Parra
   8. Damian G Zuloaga
   9. Marcos Simoes-Costa
   10. Paolo Emanuele Forni

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper studies how pheromone-sensing neurons of the vomeronasal organ establish and maintain cell identity. A role for a particular transcription factor, AP-2e, is investigated using targeted gene knockout and rescue, and observations reveal an unexpected plasticity in the cell identity of mature sensory neurons. This paper should be of broad interest to neuroscientists and cell biologists studying mechanisms of cell development and differentiation.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. Modeling and predicting the overlap of B- and T-cell receptor repertoires in healthy and SARS-CoV-2 infected individuals

   This article has 4 authors:

   1. Maria Ruiz Ortega
   2. Natanael Spisak
   3. Thierry Mora
   4. Aleksandra M. Walczak

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work is potentially very important to dissect the immune repertoire of T/B cells, which is one of the most critical/difficult parts for the adaptive immune system to achieve antigen specificity. These conclusions and proposed methods will require additional experimental support and further validation in different disease conditions.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife, ScreenIT

7. A tripartite structure, the complex nuclear receptor element (cNRE), is a c is-regulatory module of viral origin required for atrial chamber preferential gene expression

   This article has 18 authors:

   1. Luana Nunes Santos
   2. Ângela Maria da Souza Costa
   3. Martin Nikolov
   4. Allysson Coelho Sampaio
   5. Frank E. Stockdale
   6. Gang F Wangø
   7. Hozana Andrade Castillo
   8. Mariana Bortoletto Grizante
   9. Stefanie Dudczig
   10. Michelle Vasconcelos
   11. Nadia Rosenthal
   12. Patricia Regina Jusuf
   13. Paulo de Oliveira
   14. Tatiana Guimarães de Freitas Matos
   15. William Nikovits
   16. Michael Schubert
   17. Mirana Ramialison
   18. José Xavier-Neto

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this manuscript Nunes Santos et al. use a combination of computation and experimental methods to identify and characterize a cis-regulatory element that mediates expression of the quail Slow Myosin Heavy Chain III (SMyHC III) gene in the heart. The study contributes to our understanding of how genes can be expressed differentially in the atrial and ventricular chambers of the heart. The evidence for the newly-identified gene regulatory sequence, and its origin, in exclusively directing these gene expression differences could be stronger. This study is of potential interest to readers in the fields of developmental biology, evolution, gene regulation, and biology of repeats.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

8. HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages

   This article has 12 authors:

   1. Wei-Jia Luo
   2. Sung-Liang Yu
   3. Chia-Ching Chang
   4. Min-Hui Chien
   5. Ya-Ling Chang
   6. Keng-Mao Liao
   7. Pei-Chun Lin
   8. Kuei-Pin Chung
   9. Ya-Hui Chuang
   10. Jeremy JW Chen
   11. Pan-Chyr Yang
   12. Kang-Yi Su

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Wei- Jai et al., demonstrate the protein Human liver DnaJ-like protein (HLJ1) converts misfolded IL-12p35 homodimers to monomers in sepsis. This contributes to increased IL-12 activity, in macrophages which in turn leads to an increased production of IFN-gamma production and lethality in mice. This study suggests that HLJ1 plays an role in regulating IFN dependent mortality and future studies may determine a therapeutic role in blocking this protein.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. Selective endocytosis controls slit diaphragm maintenance and dynamics in Drosophila nephrocytes

   This article has 13 authors:

   1. Konrad Lang
   2. Julian Milosavljevic
   3. Helena Heinkele
   4. Mengmeng Chen
   5. Lea Gerstner
   6. Dominik Spitz
   7. Severine Kayser
   8. Martin Helmstädter
   9. Gerd Walz
   10. Michael Köttgen
   11. Andrew Spracklen
   12. John Poulton
   13. Tobias Hermle

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper would be of interest to all researchers who work in understanding the mechanisms involved in podocyte slit diaphragm homeostasis and maintenance of the glomerular filtration barrier. The work provides substantial new insights into nephrin dynamics and the mechanisms of slit diaphragm maintenance. A series of compelling experiments depicted that dynamin-mediated endocytosis was involved in ectopic nephrin turnover and that flotillin-mediated turnover of nephrin occurred within the slit diaphragm was needed to maintain filter permeability in-vivo.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

10. Box C/D small nucleolar ribonucleoproteins regulate mitochondrial surveillance and innate immunity

    This article has 3 authors:

    1. Elissa Tjahjono
    2. Alexey V. Revtovich
    3. Natalia V. Kirienko

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This manuscript addresses a potential role for the box C/D Small Nucleolar Ribonucleoprotein complex at the intersection of mitochondrial homeostasis and cellular responses to infection and environmental stress in the context of the C. elegans system. The presented data can be explained in multiple ways and can serve as starting point for further research in this interesting and important area.

      “(This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife