eLife

Latest preprint reviews

1. Selective endocytosis controls slit diaphragm maintenance and dynamics in Drosophila nephrocytes

   This article has 13 authors:

   1. Konrad Lang
   2. Julian Milosavljevic
   3. Helena Heinkele
   4. Mengmeng Chen
   5. Lea Gerstner
   6. Dominik Spitz
   7. Severine Kayser
   8. Martin Helmstädter
   9. Gerd Walz
   10. Michael Köttgen
   11. Andrew Spracklen
   12. John Poulton
   13. Tobias Hermle

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper would be of interest to all researchers who work in understanding the mechanisms involved in podocyte slit diaphragm homeostasis and maintenance of the glomerular filtration barrier. The work provides substantial new insights into nephrin dynamics and the mechanisms of slit diaphragm maintenance. A series of compelling experiments depicted that dynamin-mediated endocytosis was involved in ectopic nephrin turnover and that flotillin-mediated turnover of nephrin occurred within the slit diaphragm was needed to maintain filter permeability in-vivo.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

2. Box C/D small nucleolar ribonucleoproteins regulate mitochondrial surveillance and innate immunity

   This article has 3 authors:

   1. Elissa Tjahjono
   2. Alexey V. Revtovich
   3. Natalia V. Kirienko

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript addresses a potential role for the box C/D Small Nucleolar Ribonucleoprotein complex at the intersection of mitochondrial homeostasis and cellular responses to infection and environmental stress in the context of the C. elegans system. The presented data can be explained in multiple ways and can serve as starting point for further research in this interesting and important area.

     “(This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

3. Computational exploration of treadmilling and protrusion growth observed in fire ant rafts

   This article has 2 authors:

   1. Robert J. Wagner
   2. Franck J. Vernerey

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this study, the authors present a numerical model of ant raft shape dynamics. It is an interesting topic, the experimental movies are exciting, and the idea that ant rafts make protrusions is new. The goal seems to be to explain how local interactions can lead to the perpetual protrusions of the raft. Since the biological significance of the results has not been clarified, the paper is likely to be primarily interesting to engineers and experts on robotics.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. Toward a Universal Model for Spatially Structured Populations

   This article has 3 authors:

   1. Loïc Marrec
   2. Irene Lamberti
   3. Anne-Florence Bitbol

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This very clear paper, which will be of interest to scientists in the field of evolutionary biology, investigates the effect of population structure on the efficacy of selection on a single locus. The results are based on analytical computations and numerical simulations, conducted in a scientifically rigorous manner. Although the conclusions are currently limited, the paper could serve as a solid basis for a more ambitious investigation.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

5. TRF2-mediated ORC recruitment underlies telomere stability upon DNA replication stress

   This article has 6 authors:

   1. Mitsunori Higa
   2. Yukihiro Matsuda
   3. Jumpei Fujii
   4. Nozomi Sugimoto
   5. Kazumasa Yoshida
   6. Masatoshi Fujita

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to biochemists studying DNA replication and genome maintenance in eukaryotic cells. The work details a structure-function analysis of an interaction between two proteins that are critical for genome stability. A mutation that disrupts this interaction may have no adverse effects under unperturbed conditions but causes telomeric DNA damage when cells experience replication stress. However, the structural nature of the damage and cellular consequences are not sufficiently explored.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. Suppression of endothelial miR-22 mediates non-small cell lung cancer cell-induced angiogenesis

   This article has 13 authors:

   1. Yuan Gu
   2. Gianni Pais
   3. Vivien Becker
   4. Christina Körbel
   5. Emmanuel Ampofo
   6. Elke Ebert
   7. Johannes Hohneck
   8. Nicole Ludwig
   9. Eckart Meese
   10. Rainer M. Bohle
   11. Yingjun Zhao
   12. Michael D. Menger
   13. Matthias W. Laschke

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study reports a novel function of non-coding RNA miR-22 in the regulation of tumor-associated angiogenesis. The presented data suggest a possible link between microRNA and endothelial cell function. If the underlying mechanisms were further explored, this work would be interesting for people working on microRNA function in endothelial cells. Furthermore, considering the increasing interest of combination of anti-angiogenesis agents with anti-PD1 immunotherapy, this work may attract readers interested in immunology.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. CRISPR interference identifies vulnerable cellular pathways with bactericidal phenotypes in Mycobacterium tuberculosis

   This article has 5 authors:

   1. Matthew B. McNeil
   2. Laura M. Keighley
   3. Josephine R. Cook
   4. Chen‐Yi Cheung
   5. Gregory M. Cook

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     High-throughput approaches that accurately assess drug target vulberbility in Mycobacterium tuberculosis, the causative agent of tuberculosis, are urgently needed to develop new treatment options for this dreaded disease. This paper applies a CRISPRi based approach to investigate gene essentiality and vulnerability on a diverse set of 96 genes. While the key observations of the study support previous findings, the approach reported here is useful for identification and characterization of novel drug targets. The study will be of interest to microbiologists and those interested in diverse aspects of bacterial metabolism.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Physically interacting beta-delta pairs in the regenerating pancreas revealed by single-cell sequencing

   This article has 11 authors:

   1. Eran Yanowski
   2. Nancy S. Yacovzada
   3. Eyal David
   4. Amir Giladi
   5. Diego Jaitin
   6. Lydia Farack
   7. Adi Egozi
   8. Danny Ben-Zvi
   9. Shalev Itzkovitz
   10. Ido Amit
   11. Eran Hornstein

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper provides an attempt to understand the crosstalk between to islet cell types during beta cell regeneration following partial pancreatectomy. It combines lineage tracing, single cell sequencing and light microscopy to describe islet cell heterogeneity and interactions in the regenerating mouse pancreas. The concept of protective signaling resulting from the direct interactions between beta and delta cells is compelling and would be of interest to scientists in the field of endocrine pancreas development and regeneration. However, the conclusions derived from the sequencing data require additional experimental support.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis

   This article has 5 authors:

   1. Alexander J. Stemm-Wolf
   2. Eileen T. O’Toole
   3. Ryan M. Sheridan
   4. Jacob T. Morgan
   5. Chad G. Pearson

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study investigates how deficiency in the RNA splicing factor SON impairs centriole assembly, which may underlie ciliopathy-like phenotypes in humans with SON mutations and is thus of interest to both cell biologists and clinicians. Using RNA-sequencing analysis and advanced imaging techniques the authors discover a large number of known and new SON splicing targets and attempt to identify those crucial for SON knockdown defects. However, knockdown of a subset of targets did not fully recapitulate SON depletion phenotypes and only led to the relatively vague conclusion that the observed centriole assembly defects were caused by impaired protein trafficking around the centrosome.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. The Nse5/6-like SIMC1-SLF2 complex localizes SMC5/6 to viral replication centers

    This article has 10 authors:

    1. Martina Oravcová
    2. Minghua Nie
    3. Nicola Zilio
    4. Shintaro Maeda
    5. Yasaman Jami-Alahmadi
    6. Eros Lazzerini-Denchi
    7. James A Wohlschlegel
    8. Helle D Ulrich
    9. Takanori Otomo
    10. Michael N Boddy

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This paper will be of interest to the chromosome biology field and SMC researchers in particular. The study provides cell biological, biochemical, and structural modeling evidence that a new Nse5-like protein named SIMC1 is a paralog of SLF1, and that the two compete for SLF2-Smc5/6 binding. The authors also show that SIMC1 targets SMC5/6 to polyomavirus replication centers through its SUMO binding motifs (SIMs), supporting a role for SIMC1 in Smc5/6 recruitment for viral restriction.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife