eLife

Latest preprint reviews

1. Highly efficient generation of isogenic pluripotent stem cell models using prime editing

   This article has 11 authors:

   1. Hanqin Li
   2. Oriol Busquets
   3. Yogendra Verma
   4. Khaja Mohieddin Syed
   5. Nitzan Kutnowski
   6. Gabriella R Pangilinan
   7. Luke A Gilbert
   8. Helen S Bateup
   9. Donald C Rio
   10. Dirk Hockemeyer
   11. Frank Soldner

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this manuscript, Li et al directly compare different editing strategies for human pluripotent stem cells. They demonstrate that prime editing is more efficient and precise, compared with double-strand break-based methods. They also confirm the suitability of prime editing for the introduction of different mutations related to Parkinson's disease as a model.

     (This preprint has been reviewed by eLife. We include the joint public review from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

2. Mandrill mothers associate with infants who look like their own offspring using phenotype matching

   This article has 8 authors:

   1. Marie JE Charpentier
   2. Clémence Poirotte
   3. Berta Roura-Torres
   4. Paul Amblard-Rambert
   5. Eric Willaume
   6. Peter M Kappeler
   7. François Rousset
   8. Julien P Renoult

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This article is of potential interest to researchers working on primate social behaviour, as it presents a novel mechanism for how an association with non-relatives can be favoured under kin selection. In a wild mandrill population, mothers are observed to preferentially lead offspring to associate with paternal half-sibs, a potential mechanism for encouraging nepotistic interactions between their offspring and other members of their group. The authors' explanation for their results was considered to be only partially supported by the data and a more measured and nuanced presentation would be appropriate.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

3. β-cell deletion of the PKm1 and PKm2 isoforms of pyruvate kinase in mice reveals their essential role as nutrient sensors for the KATP channel

   This article has 12 authors:

   1. Hannah R Foster
   2. Thuong Ho
   3. Evgeniy Potapenko
   4. Sophia M Sdao
   5. Shih Ming Huang
   6. Sophie L Lewandowski
   7. Halena R VanDeusen
   8. Shawn M Davidson
   9. Rebecca L Cardone
   10. Marc Prentki
   11. Richard G Kibbey
   12. Matthew J Merrins

   This article has been curated by 1 group:

   • Curated by eLife

     **Evaluation Summary:
     **
     This manuscript employs in vitro studies and elegant mouse models to detail how specific pyruvate kinase isoforms impact pancreatic beta-cell ATP/ADP levels, ATP-sensitive K+ channel (KATP channel) activity, calcium handling, and insulin secretion. This is an important study that challenges the current paradigms of KATP-channel regulation, the major signaling mechanism through which pancreatic beta cells couple blood glucose levels to insulin release. Future studies will be necessary to determine whether similar mechanisms are used in human pancreatic beta cells.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

4. Nuclear fascin regulates cancer cell survival

   This article has 11 authors:

   1. Campbell D Lawson
   2. Samantha Peel
   3. Asier Jayo
   4. Adam Corrigan
   5. Preeti Iyer
   6. Mabel Baxter Dalrymple
   7. Richard J Marsh
   8. Susan Cox
   9. Isabel Van Audenhove
   10. Jan Gettemans
   11. Maddy Parsons

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work explores a topic of high interest to cell and cancer biologists - the role of actin polymerization, and here specifically the role of fascin, in the nucleus. The authors show that fascin regulates nuclear actin, chromatin organization, response to DNA damage, and demonstrate the need for control of steady-state nuclear levels to avoid cell death. Studying nuclear actin is technically challenging, and the authors deploy some novel technologies towards this goal. There are some very elegant experiments in this paper that suggest fascin has an important role in regulating nuclear actin and other important aspects of cancer cell behaviour. The work could be enhanced by the authors considering adding some additional experiments and providing clarifications and some further details or discussion.

     (This preprint has been reviewed by eLife. We include the joint public review from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. All three Reviewers agreed to share their names with the authors.)

   Reviewed by eLife

5. How should COVID-19 vaccines be distributed between the Global North and South: a discrete choice experiment in six European countries

   This article has 4 authors:

   1. Janina I Steinert
   2. Henrike Sternberg
   3. Giuseppe A Veltri
   4. Tim Büthe

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper provides evidence on public opinion from six European countries on key attributes according to which they believe COVID-19 vaccines should be prioritized. The paper presents significant and valuable findings supported by solid evidence.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewer remained anonymous to the authors)

   Reviewed by eLife, ScreenIT

6. Early life stressful experiences escalate aggressive behavior in adulthood via changes in transthyretin expression and function

   This article has 7 authors:

   1. Rohit Singh Rawat
   2. Aksheev Bhambri
   3. Muneesh Pal
   4. Avishek Roy
   5. Suman Jain
   6. Beena Pillai
   7. Arpita Konar

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work will be of interest to biological and clinical specialists interested in the fields of behavioural neuroscience, biological psychiatry, neuroendocrinology, and developmental psychology for its focus on the origins of adult aggressive behavior in early life stress. The authors used an unbiased transcriptomic analysis and identified the thyroid hormone system as a potential mediator of the enduring impact of early stress and aberrant aggressive behavior in adulthood.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

7. Multi-step recognition of potential 5' splice sites by the Saccharomyces cerevisiae U1 snRNP

   This article has 6 authors:

   1. Sarah R Hansen
   2. David S White
   3. Mark Scalf
   4. Ivan R Corrêa
   5. Lloyd M Smith
   6. Aaron A Hoskins

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study extends previous work from the same group on the mechanism of 5' splice site recognition using co-localization single-molecule spectroscopy. There are three important conclusions: 1) the association of the U1 snRNP with the 5' splice site is largely determined by the snRNP itself and does not require other splicing factors; 2) sequence features of the 5' splice site determine whether a short-lived complex with U1 dissociates or transitions into a longer-lived, "productive" complex, potentially mediated by stabilized contacts with U1 associated proteins; and 3) the ability to form the longer-lived complex cannot be accurately predicted by base-pairing potential alone, as presumed by many predictive algorithms. Currently, a test for the role of specific protein-RNA contacts is lacking; additionally, a comparison with other nucleic acid recognition events is missing, particularly those also showing a two-step binding mechanism. This work will be of interest to colleagues in the splicing field as well as to others in fields where nucleic acid recognition by snRNPs plays a major role.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

8. Interoperability of RTN1A in dendrite dynamics and immune functions in human Langerhans cells

   This article has 7 authors:

   1. Małgorzata Anna Cichoń
   2. Karin Pfisterer
   3. Judith Leitner
   4. Lena Wagner
   5. Clement Staud
   6. Peter Steinberger
   7. Adelheid Elbe-Bürger

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is an excellent manuscript that addresses the role of the molecule (RETICULON 1A / RTN1A) in the biology of human Langerhans cells (the epidermal resident dendritic cell). The study shows that RTN1A critically regulates the retention within the epidermis versus the emigration from the epidermis of these cells. Since Langerhans cells are central in the induction of immune responses (e.g. in vaccinations, allergic hypersensitivities) as well as in the maintenance of immunological tolerance (e.g. in autoimmune diseases of the skin) this manuscript will be of wide interest to the scientific community working in the fields of immunology/vaccinology, dermatology, cell biology and beyond.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

9. RNA localization mechanisms transcend cell morphology

   This article has 4 authors:

   1. Raeann Goering
   2. Ankita Arora
   3. Megan C Pockalny
   4. J Matthew Taliaferro

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Goering and colleagues investigate subcellular RNA localization across different cell types and species. The major insight is that there may be general mechanisms and specifically conserved proteins that regulate RNA localization in diverse cell types and morphologies. This manuscript will be of interest to those studying gene expression and how its regulation occurs within the cell.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. The proportion of randomized controlled trials that inform clinical practice

    This article has 4 authors:

    1. Nora Hutchinson
    2. Hannah Moyer
    3. Deborah A Zarin
    4. Jonathan Kimmelman

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This article constructs a four-step assessment of the informativeness of a clinical trial that measures its feasibility, reporting, importance, and risk of bias. This is a potentially highly relevant methodology for the class of trials for which it is defined, namely 'clinically directed randomized controlled trials'. It could also be translated and validated in other areas, using data from a wider set of sources beyond the trial registry clinicaltrials.gov. However, the extended longitudinal nature of the assessment and its potential subjectivity limit this tool's utility to being a retrospective diagnostic rather than as a prospective diagnostic and/or fix for at-risk designs.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. All three Reviewers agreed to share their names with the authors.)

    Reviewed by eLife