eLife

Latest preprint reviews

1. Tyrosine phosphorylation tunes chemical and thermal sensitivity of TRPV2 ion channel

   This article has 13 authors:

   1. Xiaoyi Mo
   2. Peiyuan Pang
   3. Yulin Wang
   4. Dexiang Jiang
   5. Mengyu Zhang
   6. Yang Li
   7. Peiyu Wang
   8. Qizhi Geng
   9. Chang Xie
   10. Hai-Ning Du
   11. Bo Zhong
   12. Dongdong Li
   13. Jing Yao

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The TRPV2 channel plays essential roles in many cell types in the body, including macrophages and cardiomyocytes, but its physiological mechanisms of activation and regulation remain largely unknown. Mo and collaborators describe a novel regulatory mechanism of TRPV2 channels in which phosphorylation at three different tyrosine residues by JAK1 sensitizes channels to activation by 2-APB and heat, whereas de-phosphorylation by PTPN1 reverses sensitization. Changes in the dynamics of TRPV2 channel phosphorylation could have important physiological consequences in many cell types expressing these channels. The data are of significant importance for the scientific community interested in function und relevance of transient receptor potential ion channels.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

2. Prefrontal cortex supports speech perception in listeners with cochlear implants

   This article has 8 authors:

   1. Arefeh Sherafati
   2. Noel Dwyer
   3. Aahana Bajracharya
   4. Mahlega Samira Hassanpour
   5. Adam T Eggebrecht
   6. Jill B Firszt
   7. Joseph P Culver
   8. Jonathan E Peelle

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work generates helpful new data on the network of brain areas employed for listening to speech in cochlear implant users. This is a difficult undertaking because functional MR is not possible in this group. The work uses a new type of near infrared spectroscopy to obtain measures related to brain activity in superficial areas and shows increased activity in frontal areas during speech perception.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

3. Regional opportunities for tundra conservation in the next 1000 years

   This article has 2 authors:

   1. Stefan Kruse
   2. Ulrike Herzschuh

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Kruse and Herzschuh apply LAVESI, a machine-intensive and spatially-explicit simulation of individual Siberian trees at the tundra-forest boundary, to call attention to the rapid reduction in the tundra biome as climate warming pushes forests toward the Arctic Ocean. This detailed modelling study predicts dramatic losses of tundra area by the middle of the millenium even under an ambitious climate mitigation scenario and highlights considerable risks of extinction.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

4. Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells

   This article has 11 authors:

   1. Le Xiong
   2. Erik A Tolen
   3. Jinmi Choi
   4. Sergiy Velychko
   5. Livia Caizzi
   6. Taras Velychko
   7. Kenjiro Adachi
   8. Caitlin M MacCarthy
   9. Michael Lidschreiber
   10. Patrick Cramer
   11. Hans R Schöler

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript by Xiong et al provides high resolution kinetic information on transcriptional events and enhancer activity after loss of Oct4. The authors conclude that Oct4 mainly acts as an activator whereas Sox2 maintains chromatin accessibility. These results are of interest for stem cell biologists and developmental biologists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.

   Reviewed by eLife

5. Concerted modification of nucleotides at functional centers of the ribosome revealed by single-molecule RNA modification profiling

   This article has 8 authors:

   1. Andrew D Bailey
   2. Jason Talkish
   3. Hongxu Ding
   4. Haller Igel
   5. Alejandra Duran
   6. Shreya Mantripragada
   7. Benedict Paten
   8. Manuel Ares

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this manuscript Bailey et al use single molecule RNA sequencing to dissect the functional relationships between distinct rRNA modification sites. Their method allows for the deconvolution of distinct subpopulations of rRNA and provides new insights in the installment of rRNA modifications, ribosome heterogeneity and ribosome biogenesis. The paper presents a major technological advance in mapping nucleoside modifications across single RNA molecules and identifying factors that influence these modifications.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

6. A dysmorphic mouse model reveals developmental interactions of chondrocranium and dermatocranium

   This article has 8 authors:

   1. Susan M Motch Perrine
   2. M Kathleen Pitirri
   3. Emily L Durham
   4. Mizuho Kawasaki
   5. Hao Zheng
   6. Danny Z Chen
   7. Kazuhiko Kawasaki
   8. Joan T Richtsmeier

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work combines imaging and quantitative analyses to address the conclusions that the chondrocranium and dermatocranium form an integrated unit of development and alterations in the chondrocranium drive changes in the dermatocranium. However, the manuscript in the current form suffers from data uncertainty because raw data was not provided, and the title and the conclusions go too much beyond the results they have gathered.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

7. Metabolic basis for the evolution of a common pathogenic Pseudomonas aeruginosa variant

   This article has 5 authors:

   1. Dallas L Mould
   2. Mirjana Stevanovic
   3. Alix Ashare
   4. Daniel Schultz
   5. Deborah A Hogan

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study aimed to identify the genetic foundation favoring selection of lasR mutants in laboratory and clinical isolates from persons with CF. They selected these mutants using a predictable and quantitative framework of evolution experiments and then identified their genetic underpinnings by a a suppressor screen. The role of cbrAB as a key intermediate is important and ties together several reports of nutrient-dependent advantages of lasR like phenylalanine.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

8. Plexins promote Hedgehog signaling through their cytoplasmic GAP activity

   This article has 8 authors:

   1. Justine M Pinskey
   2. Tyler M Hoard
   3. Xiao-Feng Zhao
   4. Nicole E Franks
   5. Zoë C Frank
   6. Alexandra N McMellen
   7. Roman J Giger
   8. Benjamin L Allen

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Prompted by previous findings that Neuropilins can stimulate activity of the Hh signalling pathway, the authors have investigated the possible involvement of Plexins in this process. Using NIH3T3 cells as an assay system, they present evidence that multiple Plexins are able to enhance the transcriptional response to SHH ligand and that this requires activity of the C-terminal intracellular domain of the Plexin protein. They present in vivo evidence that Plexins can stimulate and are required for HH-dependent processes in the CNS. The data are compelling and add further insights into the workings of this important signalling pathway.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

9. Integrated analyses of growth differentiation factor-15 concentration and cardiometabolic diseases in humans

   This article has 19 authors:

   1. Susanna Lemmelä
   2. Eleanor M Wigmore
   3. Christian Benner
   4. Aki S Havulinna
   5. Rachel MY Ong
   6. Tibor Kempf
   7. Kai C Wollert
   8. Stefan Blankenberg
   9. Tanja Zeller
   10. James E Peters
   11. Veikko Salomaa
   12. Maria Fritsch
   13. Ruth March
   14. Aarno Palotie
   15. Mark Daly
   16. Adam S Butterworth
   17. Mervi Kinnunen
   18. Dirk S Paul
   19. Athena Matakidou

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This integrated observational and genetic analysis using data from large biobanks comprehensively investigated the role of Growth Differentiation Factor-15 in a wide range of human diseases and will be of interest to cardiometabolic disorder researchers. The study showed that elevated blood GDF15 level in humans is not a causal factor in human cardiometabolic disease but a biomarker of metabolic stress, and BMI is a causal factor for GDF15 blood levels.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. Cd59 and inflammation regulate Schwann cell development

    This article has 5 authors:

    1. Ashtyn T Wiltbank
    2. Emma R Steinson
    3. Stacey J Criswell
    4. Melanie Piller
    5. Sarah Kucenas

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This paper by Wiltbank et al. "Cd59 and inflammation orchestrate Schwann cell development" investigates the function of the small GPI-anchored protein Cd59, a protein known to suppress complement mediated inflammation, in Schwann cell development and myelination, using zebrafish as a model system. This paper will be of broad interest to developmental biologists, glial biologists and immunologists, as it suggests the interesting and novel findings that Cd59 regulates Schwann cell development, mainly by modulating Schwann cell proliferation.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife