eLife

Latest preprint reviews

1. Dynamics of allosteric regulation of the phospholipase C-γ isozymes upon recruitment to membranes

   This article has 9 authors:

   1. Edhriz Siraliev-Perez
   2. Jordan TB Stariha
   3. Reece M Hoffmann
   4. Brenda RS Temple
   5. Qisheng Zhang
   6. Nicole Hajicek
   7. Meredith L Jenkins
   8. John E Burke
   9. John Sondek

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work provides insight into how phospholipase C gamma (PLCγ1) becomes activated upon binding to phosphorylated receptor tyrosine kinase, with an analysis of PLC γ1 bound to the soluble kinase domain of FGFR1 (FGFR1K) and/or liposomes containing PIP2. The most interesting finding is that regions of the protein far from the FGFR1K binding site increase in exchange upon binding. This is new information for a large protein that is arguably difficult to study, but it conforms to what has been observed in many other autoinhibited systems with similar SH2 and SH3 domains such as kinases. The results will be of interest to structural biologists and cell biologists with interest in the mechanisms leading to the regulation of phospholipase C activity on membranes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

2. Structural differences in adolescent brains can predict alcohol misuse

   This article has 32 authors:

   1. Roshan Prakash Rane
   2. Evert Ferdinand de Man
   3. JiHoon Kim
   4. Kai Görgen
   5. Mira Tschorn
   6. Michael A Rapp
   7. Tobias Banaschewski
   8. Arun LW Bokde
   9. Sylvane Desrivieres
   10. Herta Flor
   11. Antoine Grigis
   12. Hugh Garavan
   13. Penny A Gowland
   14. Rüdiger Brühl
   15. Jean-Luc Martinot
   16. Marie-Laure Paillere Martinot
   17. Eric Artiges
   18. Frauke Nees
   19. Dimitri Papadopoulos Orfanos
   20. Herve Lemaitre
   21. Tomas Paus
   22. Luise Poustka
   23. Juliane Fröhner
   24. Lauren Robinson
   25. Michael N Smolka
   26. Jeanne Winterer
   27. Robert Whelan
   28. Gunter Schumann
   29. Henrik Walter
   30. Andreas Heinz
   31. Kerstin Ritter
   32. IMAGEN consortium

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study uses a large dataset on alcohol misuse in adolescents that have been followed up for several years. MRI data are used to test whether the structure and connectivity of the brains of adolescents can predict their alcohol misuse later in their early twenties. The results show that binge drinking can be predicted out of multiple brain phenotypes with a good accuracy, even after control for many confound variables. This study can be impactful as it suggests a reevaluation of studies of the effect of alcohol on the adolescent brain.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

3. Glutathione in the nucleus accumbens regulates motivation to exert reward-incentivized effort

   This article has 12 authors:

   1. Ioannis Zalachoras
   2. Eva Ramos-Fernández
   3. Fiona Hollis
   4. Laura Trovo
   5. João Rodrigues
   6. Alina Strasser
   7. Olivia Zanoletti
   8. Pascal Steiner
   9. Nicolas Preitner
   10. Lijing Xin
   11. Simone Astori
   12. Carmen Sandi

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study uses both humans and rats to demonstrate that the level of glutathione in the nucleus accumbens correlates with effortful behaviors. The authors provide causal evidence for glutathione in rats by manipulating enzymes involved in the synthesis of glutathione. Although how exactly glutathione regulates effort-related behavior remains to be clarified, overall, the study gives convincing evidence for an important role of glutathione in nucleus accumbens in regulating the willingness to invest effort to obtain reward or escape an aversive situation.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

4. Hypertrophic cardiomyopathy mutations in the pliant and light chain-binding regions of the lever arm of human β-cardiac myosin have divergent effects on myosin function

   This article has 6 authors:

   1. Makenna M Morck
   2. Debanjan Bhowmik
   3. Divya Pathak
   4. Aminah Dawood
   5. James Spudich
   6. Kathleen M Ruppel

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work is of broad interest to readers in the fields of cytoskeletal research, muscle biology and heart disease. By utilizing a combination of quantitative biochemical and biophysical experimental approaches, this work provides critical new insights into the molecular mechanisms of understudied mutations in myosin that cause heart disease. The data are rigorously controlled and analyzed and support the claims of the work.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. Endocytic trafficking promotes vacuolar enlargements for fast cell expansion rates in plants

   This article has 11 authors:

   1. Kai Dünser
   2. Maria Schöller
   3. Ann-Kathrin Rößling
   4. Christian Löfke
   5. Nannan Xiao
   6. Barbora Pařízková
   7. Stanislav Melnik
   8. Marta Rodriguez-Franco
   9. Eva Stöger
   10. Ondřej Novák
   11. Jürgen Kleine-Vehn

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Plant cells can grow to extraordinarily large volumes; Arabidopsis root cells, for example, can expand beyond 50um long. Vacuole expansion is correlated with cell elongation, presumably to "fill up" the volume of the cell without requiring a tremendous volume of cytoplasm. Here, the authors carefully characterize a new small molecule inhibitor of endocytic trafficking to the vacuole. This new tool will be valuable to researchers studying endocytic trafficking and vacuole biogenesis in plants.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

6. Uncovering natural variation in root system architecture and growth dynamics using a robotics-assisted phenomics platform

   This article has 6 authors:

   1. Therese LaRue
   2. Heike Lindner
   3. Ankit Srinivas
   4. Moises Exposito-Alonso
   5. Guillaume Lobet
   6. José R Dinneny

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors present an automated system for phenotyping root system architecture based on bioluminescent roots resulting from a constitutively expressed luciferase transgene (GLO-Root). They have developed a robotics-assisted phenotyping platform and an automated image analysis pipeline for high throughput analysis. An impressive array of 93 luciferase expressing Arabidopsis thaliana accessions provides a major resource for understanding the genetic basis for root system architecture variation in response to a range of environmental conditions. The work will be of interest to plant biologists and all those studying genetic variation in plants.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

7. Fat2 polarizes the WAVE complex in trans to align cell protrusions for collective migration

   This article has 4 authors:

   1. Audrey Miller Williams
   2. Seth Donoughe
   3. Edwin Munro
   4. Sally Horne-Badovinac

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of broad interest to scientists who study collective cell migration and cytoskeletal dynamics. The findings of this paper connect proteins involved in planar polarity to the actin protrusive machinery which establishes an axes for polarized collective cell migration. The data presented largely supports the claims of the authors who take advantage of quantitative imaging techniques and Drosophila genetics to establish this connection.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Binary and analog variation of synapses between cortical pyramidal neurons

   This article has 37 authors:

   1. Sven Dorkenwald
   2. Nicholas L Turner
   3. Thomas Macrina
   4. Kisuk Lee
   5. Ran Lu
   6. Jingpeng Wu
   7. Agnes L Bodor
   8. Adam A Bleckert
   9. Derrick Brittain
   10. Nico Kemnitz
   11. William M Silversmith
   12. Dodam Ih
   13. Jonathan Zung
   14. Aleksandar Zlateski
   15. Ignacio Tartavull
   16. Szi-Chieh Yu
   17. Sergiy Popovych
   18. William Wong
   19. Manuel Castro
   20. Chris S Jordan
   21. Alyssa M Wilson
   22. Emmanouil Froudarakis
   23. JoAnn Buchanan
   24. Marc M Takeno
   25. Russel Torres
   26. Gayathri Mahalingam
   27. Forrest Collman
   28. Casey M Schneider-Mizell
   29. Daniel J Bumbarger
   30. Yang Li
   31. Lynne Becker
   32. Shelby Suckow
   33. Jacob Reimer
   34. Andreas S Tolias
   35. Nuno Macarico da Costa
   36. R Clay Reid
   37. H Sebastian Seung

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Synaptic connections are crucial for determining neural circuit function and for storing adaptive changes in the brain. This study performs a highly detailed quantitative analysis of certain excitatory connections in mouse neocortex, and finds that the physical size of these connections has a bimodal distribution. This is an important finding that has implications for our understanding of synaptic plasticity and neural circuit dynamics.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

9. Decoding the IGF1 signaling gene regulatory network behind alveologenesis from a mouse model of bronchopulmonary dysplasia

   This article has 10 authors:

   1. Feng Gao
   2. Changgong Li
   3. Susan M Smith
   4. Neil Peinado
   5. Golenaz Kohbodi
   6. Evelyn Tran
   7. Yong-Hwee Eddie Loh
   8. Wei Li
   9. Zea Borok
   10. Parviz Minoo

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to lung biologists, developmental biologists, and neonatologists interested in lung injury. In this manuscript, the authors used gene expression signatures to construct a gene regulatory network to identify genes associated with alveologenesis. While reviewers were impressed with the novelty of the approach, questions were raised about the robustness of the results in mice and the validation in human samples.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. Non-invasive classification of macrophage polarisation by 2P-FLIM and machine learning

    This article has 6 authors:

    1. Nuno GB Neto
    2. Sinead A O'Rourke
    3. Mimi Zhang
    4. Hannah K Fitzgerald
    5. Aisling Dunne
    6. Michael G Monaghan

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      Neto et al set out to use Two-Photon FLIM and machine learning to classify macrophages that are polarised along the M1/M2 axis and then subjected to different metabolic stresses classically used to distinguish metabolic strategies of different cell states. Additional information is sought regarding the photophysics of the measurements and if there are an adequate number of photons to fairly compare the three conditions. The work will be of interest to immunologists, physiologists interested in metabolism and engineers looking to translate the findings.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

    Reviewed by eLife