eLife

Latest preprint reviews

1. A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation

   This article has 6 authors:

   1. Andrew Loffer
   2. Jasleen Singh
   3. Akihito Fukudome
   4. Vibhor Mishra
   5. Feng Wang
   6. Craig S Pikaard

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The paper is of interest to RNA biologists, especially to those who study small RNAs. The findings deepen our understanding of the rules of DCL3 dicing and explain how 23-nt and 24-nt siRNAs in the RNA-directed DNA methylation pathway are produced. Overall, the data are of high quality and support the paper's conclusions.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

2. An estimate of the deepest branches of the tree of life from ancient vertically evolving genes

   This article has 9 authors:

   1. Edmund RR Moody
   2. Tara A Mahendrarajah
   3. Nina Dombrowski
   4. James W Clark
   5. Celine Petitjean
   6. Pierre Offre
   7. Gergely J Szöllősi
   8. Anja Spang
   9. Tom A Williams

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This contribution is of interest to molecular phylogeny scientists in particular and to a broad public interested in early evolution in general, as it confirms the long-standing (but recently challenged) assumption that bacteria and archaea are separated by a long branch. It elegantly rebuts a recent study claiming that one of the common markers used for molecular evolution, ribosomal proteins, are actually ill-suited for deep phylogenies and that archaea and bacteria are much closer to each other than previously thought.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

3. The anterior paired lateral neuron normalizes odour-evoked activity in the Drosophila mushroom body calyx

   This article has 5 authors:

   1. Luigi Prisco
   2. Stephan Hubertus Deimel
   3. Hanna Yeliseyeva
   4. André Fiala
   5. Gaia Tavosanis

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors show that a widely arborizing inhibitory interneuron AL reduces the variability of olfactory responses in at Drosophila Kenyon postsynapses in the mushroom body calyx, which receive inputs from the projection neurons (PNs) in the antennal lobe. The data support a model in which APL neurons, which also receive inputs from PNs, mediate scaled GABAergic feedback to normalize of postsynaptic responses in the calyx. The conclusions, based on detailed analysis of one odorant are mostly well supported by the data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. Dedicated chaperones coordinate co-translational regulation of ribosomal protein production with ribosome assembly to preserve proteostasis

   This article has 7 authors:

   1. Benjamin Pillet
   2. Alfonso Méndez-Godoy
   3. Guillaume Murat
   4. Sébastien Favre
   5. Michael Stumpe
   6. Laurent Falquet
   7. Dieter Kressler

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Ribosomal proteins are prone to aggregation. This work provides strong experimental support for a novel mechanism by which the level of the mRNA encoding two different ribosomal proteins is fine-tuned by variation in the abundance or availability of their chaperones. This regulation is proposed to operate through mRNA degradation at the translating ribosome. The data are extensive - from genetic observations that hint at the existence of a feedback loop, through interaction mechanisms, to biological significance. The logical links between the different steps in the analysis are clear, sound and well set out. The conclusions are unanticipated, but convincingly supported and very likely relevant and important for other systems beyond yeast.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. Cannabinoid signaling modulation through JZL184 restores key phenotypes of a mouse model for Williams–Beuren syndrome

   This article has 17 authors:

   1. Alba Navarro-Romero
   2. Lorena Galera-López
   3. Paula Ortiz-Romero
   4. Alberto Llorente-Ovejero
   5. Lucía de los Reyes-Ramírez
   6. Iker Bengoetxea de Tena
   7. Anna Garcia-Elias
   8. Aleksandra Mas-Stachurska
   9. Marina Reixachs-Solé
   10. Antoni Pastor
   11. Rafael de la Torre
   12. Rafael Maldonado
   13. Begoña Benito
   14. Eduardo Eyras
   15. Rafael Rodríguez-Puertas
   16. Victoria Campuzano
   17. Andres Ozaita

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study gives us information about the potential of the endocannabinoid system to become a novel target for the treatment of Williams-Beuren syndrome. The authors found there is an alteration of brain cannabinoid type-1 receptor (CB1R) in a mouse model of Williams-Beuren syndrome (CD mice). Modulation of CB1R by JZL184 treatment improved social and cognitive phenotypes and also cardiac function of CD mice. This study will be of great interest to researchers and clinicians in the field of genetic diseases.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

6. Urinary metabolic biomarkers of diet quality in European children are associated with metabolic health

   This article has 35 authors:

   1. Nikos Stratakis
   2. Alexandros P Siskos
   3. Eleni Papadopoulou
   4. Anh N Nguyen
   5. Yinqi Zhao
   6. Katerina Margetaki
   7. Chung-Ho E Lau
   8. Muireann Coen
   9. Lea Maitre
   10. Silvia Fernández-Barrés
   11. Lydiane Agier
   12. Sandra Andrusaityte
   13. Xavier Basagaña
   14. Anne Lise Brantsaeter
   15. Maribel Casas
   16. Serena Fossati
   17. Regina Grazuleviciene
   18. Barbara Heude
   19. Rosemary RC McEachan
   20. Helle Margrete Meltzer
   21. Christopher Millett
   22. Fernanda Rauber
   23. Oliver Robinson
   24. Theano Roumeliotaki
   25. Eva Borras
   26. Eduard Sabidó
   27. Jose Urquiza
   28. Marina Vafeiadi
   29. Paolo Vineis
   30. Trudy Voortman
   31. John Wright
   32. David V Conti
   33. Martine Vrijheid
   34. Hector C Keun
   35. Leda Chatzi

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This well-executed study looks at the association of urinary metabolites to the types of diets consumed by European children. Using NMR they find four metabolites that are predictive of a Mediterranean diet. This presents both an approach additional to traditional dietary questionnaire methods and potential insights into biological pathways and will be of interest to nutritionists and epidemiologists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewer remained anonymous to the authors.)

   Reviewed by eLife

7. A molecular mechanism for the generation of ligand-dependent differential outputs by the epidermal growth factor receptor

   This article has 7 authors:

   1. Yongjian Huang
   2. Jana Ognjenovic
   3. Deepti Karandur
   4. Kate Miller
   5. Alan Merk
   6. Sriram Subramaniam
   7. John Kuriyan

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     By revealing different conformational landscapes of EGFR when responding to different types of ligands, this study significantly advances our understanding the structural basis for how EGFR generates distinct downstream signaling in response to different types of ligands. This study represents an important advance in the field and paves the way for the comprehensive understanding of structural mechanisms underlying biased agonism in EGFR and other RTKs.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

8. Thymic macrophages consist of two populations with distinct localization and origin

   This article has 11 authors:

   1. Tyng-An Zhou
   2. Hsuan-Po Hsu
   3. Yueh-Hua Tu
   4. Hui-Kuei Cheng
   5. Chih-Yu Lin
   6. Nien-Jung Chen
   7. Jin-Wu Tsai
   8. Ellen A Robey
   9. Hsuan-Cheng Huang
   10. Chia-Lin Hsu
   11. Ivan L Dzhagalov

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors comprehensively dissected the ontogeny and characteristics of thymic macrophages. These findings are helpful for better understanding of the function of macrophages in thymic tissue environment.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

9. Cross-reactive antibodies after SARS-CoV-2 infection and vaccination

   This article has 30 authors:

   1. Marloes Grobben
   2. Karlijn van der Straten
   3. Philip JM Brouwer
   4. Mitch Brinkkemper
   5. Pauline Maisonnasse
   6. Nathalie Dereuddre-Bosquet
   7. Brent Appelman
   8. AH Ayesha Lavell
   9. Lonneke A van Vught
   10. Judith A Burger
   11. Meliawati Poniman
   12. Melissa Oomen
   13. Dirk Eggink
   14. Tom PL Bijl
   15. Hugo DG van Willigen
   16. Elke Wynberg
   17. Bas J Verkaik
   18. Orlane JA Figaroa
   19. Peter J de Vries
   20. Tessel M Boertien
   21. Amsterdam UMC COVID-19 S3/HCW study group
   22. Marije K Bomers
   23. Jonne J Sikkens
   24. Roger Le Grand
   25. Menno D de Jong
   26. Maria Prins
   27. Amy W Chung
   28. Godelieve J de Bree
   29. Rogier W Sanders
   30. Marit J van Gils

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study examines whether binding antibodies that cross-react with the spikes of diverse coronaviruses are elicited by SARS-CoV-2 infection. The manuscript is well written, and the figures are laid out in an easy to interpret manner. This study will be of interest to those who are interested in developing pan coronavirus vaccines.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife, ScreenIT

10. Chromatin topology defines estradiol-primed progesterone receptor and PAX2 binding in endometrial cancer cells

    This article has 16 authors:

    1. Alejandro La Greca
    2. Nicolás Bellora
    3. François Le Dily
    4. Rodrigo Jara
    5. Ana Silvina Nacht
    6. Javier Quilez Oliete
    7. José Luis Villanueva
    8. Enrique Vidal
    9. Gabriela Merino
    10. Cristóbal Fresno
    11. Inti Tarifa Reischle
    12. Griselda Vallejo
    13. Guillermo Vicent
    14. Elmer Fernández
    15. Miguel Beato
    16. Patricia Saragüeta

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      All three reviewers are in agreement that the study is of potential interest in the field of ER/PR signaling and endometrial cancer and that it contains significant amount of genomic data. However, functional data linking PAX2 to the PR/ER pathway are lacking, and the study is limited to a single model cell line and thus has a relatively narrow scope. There is also a concern that ChIP-seq data appear to be from a single, unreplicated experiment.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife